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# Path Configuration
from tools.preprocess import *
# Processing context
trait = "Rectal_Cancer"
cohort = "GSE145037"
# Input paths
in_trait_dir = "../DATA/GEO/Rectal_Cancer"
in_cohort_dir = "../DATA/GEO/Rectal_Cancer/GSE145037"
# Output paths
out_data_file = "./output/preprocess/3/Rectal_Cancer/GSE145037.csv"
out_gene_data_file = "./output/preprocess/3/Rectal_Cancer/gene_data/GSE145037.csv"
out_clinical_data_file = "./output/preprocess/3/Rectal_Cancer/clinical_data/GSE145037.csv"
json_path = "./output/preprocess/3/Rectal_Cancer/cohort_info.json"
# Get file paths
soft_file_path, matrix_file_path = geo_get_relevant_filepaths(in_cohort_dir)
# Get background info and clinical data
background_info, clinical_data = get_background_and_clinical_data(matrix_file_path)
print("Background Information:")
print(background_info)
print("\nSample Characteristics:")
# Get dictionary of unique values per row
unique_values_dict = get_unique_values_by_row(clinical_data)
for row, values in unique_values_dict.items():
print(f"\n{row}:")
print(values)
# 1. Gene Expression Data Availability
# From background info, this dataset contains gene expression data from rectal cancer patients
is_gene_available = True
# 2. Variable Availability and Data Type Conversion
# 2.1 Row identifiers
trait_row = 3 # response to CRT in row 3
age_row = 2 # age in row 2
gender_row = 1 # gender in row 1
# 2.2 Conversion functions
def convert_trait(x):
if not x:
return None
value = x.split(': ')[1].lower()
return 0 if 'non-response' in value else 1
def convert_age(x):
if not x:
return None
try:
return int(x.split(': ')[1])
except:
return None
def convert_gender(x):
if not x:
return None
value = x.split(': ')[1].lower()
return 1 if 'male' in value else 0
# 3. Save metadata for initial filtering
is_trait_avail = trait_row is not None
validate_and_save_cohort_info(is_final=False,
cohort=cohort,
info_path=json_path,
is_gene_available=is_gene_available,
is_trait_available=is_trait_avail)
# 4. Extract clinical features
selected_clinical = geo_select_clinical_features(clinical_data,
trait="Response",
trait_row=trait_row,
convert_trait=convert_trait,
age_row=age_row,
convert_age=convert_age,
gender_row=gender_row,
convert_gender=convert_gender)
# Preview the extracted data
preview_dict = preview_df(selected_clinical)
print("Preview of extracted clinical data:")
print(preview_dict)
# Save clinical data
selected_clinical.to_csv(out_clinical_data_file)
# Get gene expression data from matrix file
genetic_data = get_genetic_data(matrix_file_path)
# Examine data structure
print("Data structure and head:")
print(genetic_data.head())
print("\nShape:", genetic_data.shape)
print("\nFirst 20 row IDs (gene/probe identifiers):")
print(list(genetic_data.index)[:20])
# Get a few column names to verify sample IDs
print("\nFirst 5 column names:")
print(list(genetic_data.columns)[:5])
# The IDs are human gene symbols (e.g. A1BG, A1CF, A2M) and do not need mapping
requires_gene_mapping = False
# Reload clinical data that was processed earlier
selected_clinical_df = pd.read_csv(out_clinical_data_file, index_col=0)
# 1. Normalize gene symbols
genetic_data = normalize_gene_symbols_in_index(genetic_data)
genetic_data.to_csv(out_gene_data_file)
# 2. Link clinical and genetic data
linked_data = geo_link_clinical_genetic_data(selected_clinical_df, genetic_data)
# 3. Handle missing values systematically
linked_data = handle_missing_values(linked_data, "Response")
# 4. Check for bias in trait and demographic features
trait_biased, linked_data = judge_and_remove_biased_features(linked_data, "Response")
# 5. Final validation and information saving
note = "Dataset contains gene expression data from rectal cancer patients examining chemoradiotherapy response."
is_usable = validate_and_save_cohort_info(
is_final=True,
cohort=cohort,
info_path=json_path,
is_gene_available=True,
is_trait_available=True,
is_biased=trait_biased,
df=linked_data,
note=note
)
# 6. Save linked data only if usable
if is_usable:
os.makedirs(os.path.dirname(out_data_file), exist_ok=True)
linked_data.to_csv(out_data_file) |