p3/preprocess/Thyroid_Cancer/code/GSE82208.py

# Path Configuration
from tools.preprocess import *

# Processing context
trait = "Thyroid_Cancer"
cohort = "GSE82208"

# Input paths
in_trait_dir = "../DATA/GEO/Thyroid_Cancer"
in_cohort_dir = "../DATA/GEO/Thyroid_Cancer/GSE82208"

# Output paths
out_data_file = "./output/preprocess/3/Thyroid_Cancer/GSE82208.csv"
out_gene_data_file = "./output/preprocess/3/Thyroid_Cancer/gene_data/GSE82208.csv"
out_clinical_data_file = "./output/preprocess/3/Thyroid_Cancer/clinical_data/GSE82208.csv"
json_path = "./output/preprocess/3/Thyroid_Cancer/cohort_info.json"

# Get file paths for SOFT and matrix files
soft_file_path, matrix_file_path = geo_get_relevant_filepaths(in_cohort_dir)

# Get background info and clinical data from the matrix file
background_info, clinical_data = get_background_and_clinical_data(matrix_file_path)

# Create dictionary of unique values for each feature
unique_values_dict = get_unique_values_by_row(clinical_data)

# Print the information
print("Dataset Background Information:")
print(background_info)
print("\nSample Characteristics:")
for feature, values in unique_values_dict.items():
    print(f"\n{feature}:")
    print(values)
# 1. Gene Expression Data Availability
# Series title and summary indicate gene expression data 
is_gene_available = True

# 2. Variable Availability and Data Type Conversion
# 2.1 Row Identification
trait_row = 2  # 'class' indicates FTC vs FTA
age_row = 1    # age in years available
gender_row = 0  # sex information available

# 2.2 Data Type Conversion Functions
def convert_trait(x: str) -> int:
    """Convert FTC/FTA to binary (FTC=1, FTA=0)"""
    if not isinstance(x, str):
        return None
    x = x.split(': ')[-1]
    if x == 'FTC':
        return 1
    elif x == 'FTA':
        return 0
    return None

def convert_age(x: str) -> float:
    """Convert age string to float"""
    if not isinstance(x, str):
        return None
    x = x.split(': ')[-1]
    try:
        return float(x)
    except:
        return None

def convert_gender(x: str) -> int:
    """Convert gender to binary (Female=0, Male=1)"""
    if not isinstance(x, str):
        return None
    x = x.split(': ')[-1]
    if x == 'Female':
        return 0
    elif x == 'Male':
        return 1
    return None

# 3. Save Metadata
validate_and_save_cohort_info(
    is_final=False,
    cohort=cohort,
    info_path=json_path,
    is_gene_available=is_gene_available,
    is_trait_available=trait_row is not None
)

# 4. Clinical Feature Extraction
if trait_row is not None:
    clinical_features = geo_select_clinical_features(
        clinical_df=clinical_data,
        trait=trait,
        trait_row=trait_row,
        convert_trait=convert_trait,
        age_row=age_row,
        convert_age=convert_age,
        gender_row=gender_row,
        convert_gender=convert_gender
    )
    
    # Preview the extracted features
    preview = preview_df(clinical_features)
    print("Clinical features preview:", preview)
    
    # Save to CSV
    clinical_features.to_csv(out_clinical_data_file)
# Extract genetic data matrix
genetic_data = get_genetic_data(matrix_file_path)

# Print first few rows with column names to examine data structure
print("Data preview:")
print("\nColumn names:")
print(list(genetic_data.columns)[:5])
print("\nFirst 5 rows:")
print(genetic_data.head())
print("\nShape:", genetic_data.shape)

# Verify this is gene expression data and check identifiers
is_gene_available = True

# Save updated metadata
validate_and_save_cohort_info(
    is_final=False,
    cohort=cohort,
    info_path=json_path, 
    is_gene_available=is_gene_available,
    is_trait_available=(trait_row is not None)
)

# Save gene expression data 
genetic_data.to_csv(out_gene_data_file)
requires_gene_mapping = True
# These appear to be Affymetrix probe IDs like "1007_s_at" rather than gene symbols,
# so they will need to be mapped to human gene symbols for analysis
# Extract gene annotation data
gene_metadata = get_gene_annotation(soft_file_path) 

# Preview column names and first few values
preview = preview_df(gene_metadata)
print("\nGene annotation columns and sample values:")
print(preview)
# Extract gene mapping from annotation data
prob_col = 'ID'  # Column containing probe IDs (matches gene expression data indices)
gene_col = 'Gene Symbol'  # Column containing gene symbols
mapping_data = get_gene_mapping(gene_metadata, prob_col, gene_col)

# Convert probe expressions to gene expressions by applying the mapping
gene_data = apply_gene_mapping(genetic_data, mapping_data)

# Preview the mapped data
print("Gene expression data preview:")
print("\nShape:", gene_data.shape)
print("\nFirst few genes and their expression values:")
print(gene_data.head())
# 1. Normalize gene symbols and save gene data
normalized_gene_data = normalize_gene_symbols_in_index(gene_data)
os.makedirs(os.path.dirname(out_gene_data_file), exist_ok=True)
normalized_gene_data.to_csv(out_gene_data_file)

# 2. Link clinical and genetic data  
clinical_features = pd.read_csv(out_clinical_data_file, index_col=0)
linked_data = geo_link_clinical_genetic_data(clinical_features, normalized_gene_data)

# 3. Handle missing values
linked_data = handle_missing_values(linked_data, trait)

# 4. Judge bias in features and remove biased ones
trait_biased, linked_data = judge_and_remove_biased_features(linked_data, trait)

# 5. Final validation and save metadata
is_usable = validate_and_save_cohort_info(
    is_final=True,
    cohort=cohort,
    info_path=json_path,
    is_gene_available=is_gene_available, 
    is_trait_available=True,
    is_biased=trait_biased,
    df=linked_data,
    note="Dataset contains gene expression data comparing 27 follicular thyroid cancers with 25 follicular thyroid adenomas."
)

# 6. Save linked data if usable
if is_usable:
    os.makedirs(os.path.dirname(out_data_file), exist_ok=True)
    linked_data.to_csv(out_data_file)