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diff --git a/p1/preprocess/Lung_Cancer/gene_data/GSE249568.csv b/p1/preprocess/Lung_Cancer/gene_data/GSE249568.csv new file mode 100644 index 0000000000000000000000000000000000000000..5e354574b8210872d0f62c3d5b64b53099c63947 --- /dev/null +++ b/p1/preprocess/Lung_Cancer/gene_data/GSE249568.csv @@ -0,0 +1 @@ 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diff --git a/p1/preprocess/Lung_Cancer/gene_data/TCGA.csv b/p1/preprocess/Lung_Cancer/gene_data/TCGA.csv new file mode 100644 index 0000000000000000000000000000000000000000..5244d7559b4eb4762c1af02715681132ff2961ae --- /dev/null +++ b/p1/preprocess/Lung_Cancer/gene_data/TCGA.csv @@ -0,0 +1,9 @@ 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diff --git a/p1/preprocess/Melanoma/clinical_data/GSE148319.csv b/p1/preprocess/Melanoma/clinical_data/GSE148319.csv new file mode 100644 index 0000000000000000000000000000000000000000..8bdf9cb7e60c8cc0c7dda1b8ee2672fae1952f50 --- /dev/null +++ b/p1/preprocess/Melanoma/clinical_data/GSE148319.csv @@ -0,0 +1,2 @@ +GSM4460266,GSM4460267,GSM4460268,GSM4460269,GSM4460270,GSM4460271,GSM4460272,GSM4460273,GSM4460274,GSM4460275,GSM4460276,GSM4460277,GSM4460278,GSM4460279,GSM4460280,GSM4460281,GSM4460282,GSM4460283,GSM4460284,GSM4460285,GSM4460286,GSM4460287,GSM4460288,GSM4460289,GSM4460290,GSM4460291,GSM4460292,GSM4460293,GSM4460294,GSM4460295,GSM4460296,GSM4460297,GSM4460298,GSM4460299,GSM4460300,GSM4460301,GSM4460302,GSM4460303,GSM4460304,GSM4460305,GSM4460306,GSM4460307,GSM4460308,GSM4460309,GSM4460310,GSM4460311,GSM4460312,GSM4460313,GSM4460314,GSM4460315,GSM4460316,GSM4460317,GSM4460318,GSM4460319,GSM4460320,GSM4460321,GSM4460322,GSM4460323,GSM4460324,GSM4460325,GSM4460326,GSM4460327,GSM4460328,GSM4460329,GSM4460330,GSM4460331,GSM4460332,GSM4460333,GSM4460334,GSM4460335,GSM4460336,GSM4460337,GSM4460338,GSM4460339,GSM4460340,GSM4460341,GSM4460342,GSM4460343,GSM4460344,GSM4460345,GSM4460346,GSM4460347,GSM4460348 +0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,1.0,1.0,1.0,1.0,1.0,1.0,1.0,1.0,1.0,1.0,1.0,1.0,1.0,1.0,1.0,1.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0 diff --git a/p1/preprocess/Melanoma/code/GSE144296.py b/p1/preprocess/Melanoma/code/GSE144296.py new file mode 100644 index 0000000000000000000000000000000000000000..0a20af37758799ca6d2dd431d8514d0922c21c42 --- /dev/null +++ b/p1/preprocess/Melanoma/code/GSE144296.py @@ -0,0 +1,117 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Melanoma" +cohort = "GSE144296" + +# Input paths +in_trait_dir = "../DATA/GEO/Melanoma" +in_cohort_dir = "../DATA/GEO/Melanoma/GSE144296" + +# Output paths +out_data_file = "./output/preprocess/1/Melanoma/GSE144296.csv" +out_gene_data_file = "./output/preprocess/1/Melanoma/gene_data/GSE144296.csv" +out_clinical_data_file = "./output/preprocess/1/Melanoma/clinical_data/GSE144296.csv" +json_path = "./output/preprocess/1/Melanoma/cohort_info.json" + +# STEP1 +from tools.preprocess import * + +# 1. Attempt to identify the paths to the SOFT file and the matrix file +try: + soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) +except AssertionError: + print("[WARNING] Could not find the expected '.soft' or '.matrix' files in the directory.") + soft_file, matrix_file = None, None + +if soft_file is None or matrix_file is None: + print("[ERROR] Required GEO files are missing. Please check file names in the cohort directory.") +else: + # 2. Read the matrix file to obtain background information and sample characteristics data + background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] + clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] + background_info, clinical_data = get_background_and_clinical_data(matrix_file, + background_prefixes, + clinical_prefixes) + + # 3. Obtain the sample characteristics dictionary from the clinical dataframe + sample_characteristics_dict = get_unique_values_by_row(clinical_data) + + # 4. Explicitly print out all the background information and the sample characteristics dictionary + print("Background Information:") + print(background_info) + print("\nSample Characteristics Dictionary:") + print(sample_characteristics_dict) +# 1. Gene Expression Data Availability +is_gene_available = True # Based on DNTR-seq description providing mRNA expression + +# 2. Variable Availability and Data Type Conversion +trait_row = 1 # "cell type: malignant melanoma" or "cell type: colorectal carcinoma" +age_row = None # No age information detected +gender_row = None # No gender information detected + +def convert_trait(value: str): + parts = value.split(":", 1) + if len(parts) < 2: + return None + val = parts[1].strip().lower() + if "malignant melanoma" in val: + return 1 + elif "colorectal carcinoma" in val: + return 0 + return None + +def convert_age(value: str): + # Not used here, but defined for completeness + parts = value.split(":", 1) + if len(parts) < 2: + return None + val = parts[1].strip() + try: + return float(val) + except ValueError: + return None + +def convert_gender(value: str): + # Not used here, but defined for completeness + parts = value.split(":", 1) + if len(parts) < 2: + return None + val = parts[1].strip().lower() + if val in ["male", "m"]: + return 1 + elif val in ["female", "f"]: + return 0 + return None + +# 3. Save Metadata (Initial Filtering) +is_trait_available = (trait_row is not None) +is_usable = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4. Clinical Feature Extraction +if trait_row is not None: + selected_clinical_df = geo_select_clinical_features( + clinical_data, + trait=trait, + trait_row=trait_row, + convert_trait=convert_trait, + age_row=age_row, + convert_age=convert_age, + gender_row=gender_row, + convert_gender=convert_gender + ) + previewed_data = preview_df(selected_clinical_df, n=5) + selected_clinical_df.to_csv(out_clinical_data_file) +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) \ No newline at end of file diff --git a/p1/preprocess/Melanoma/code/GSE146264.py b/p1/preprocess/Melanoma/code/GSE146264.py new file mode 100644 index 0000000000000000000000000000000000000000..c59216c632e67a232eee3ce13825c05108fff31e --- /dev/null +++ b/p1/preprocess/Melanoma/code/GSE146264.py @@ -0,0 +1,124 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Melanoma" +cohort = "GSE146264" + +# Input paths +in_trait_dir = "../DATA/GEO/Melanoma" +in_cohort_dir = "../DATA/GEO/Melanoma/GSE146264" + +# Output paths +out_data_file = "./output/preprocess/1/Melanoma/GSE146264.csv" +out_gene_data_file = "./output/preprocess/1/Melanoma/gene_data/GSE146264.csv" +out_clinical_data_file = "./output/preprocess/1/Melanoma/clinical_data/GSE146264.csv" +json_path = "./output/preprocess/1/Melanoma/cohort_info.json" + +# STEP1 +from tools.preprocess import * + +# 1. Attempt to identify the paths to the SOFT file and the matrix file +try: + soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) +except AssertionError: + print("[WARNING] Could not find the expected '.soft' or '.matrix' files in the directory.") + soft_file, matrix_file = None, None + +if soft_file is None or matrix_file is None: + print("[ERROR] Required GEO files are missing. Please check file names in the cohort directory.") +else: + # 2. Read the matrix file to obtain background information and sample characteristics data + background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] + clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] + background_info, clinical_data = get_background_and_clinical_data(matrix_file, + background_prefixes, + clinical_prefixes) + + # 3. Obtain the sample characteristics dictionary from the clinical dataframe + sample_characteristics_dict = get_unique_values_by_row(clinical_data) + + # 4. Explicitly print out all the background information and the sample characteristics dictionary + print("Background Information:") + print(background_info) + print("\nSample Characteristics Dictionary:") + print(sample_characteristics_dict) +# 1. Gene Expression Data Availability +# Based on the background info indicating single-cell RNA-seq, we set is_gene_available to True. +is_gene_available = True + +# 2. Variable Availability and Data Type Conversion +# From the sample characteristics dictionary, we see no mention of "Melanoma," age, or gender. +# Therefore, we conclude these variables are not available (None). +trait_row = None +age_row = None +gender_row = None + +# Nonetheless, define the required converter functions (they will not be used because rows are None). + +def convert_trait(x: str) -> int: + # Placeholder: no real conversion as trait data is unavailable + return None + +def convert_age(x: str) -> float: + # Placeholder: no real conversion as age data is unavailable + return None + +def convert_gender(x: str) -> int: + # Placeholder: no real conversion as gender data is unavailable + return None + +# 3. Save Metadata with initial filtering +# Trait data is considered unavailable (trait_row is None). +is_trait_available = (trait_row is not None) + +validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4. Clinical Feature Extraction +# Since trait_row is None, we skip this step (no clinical data extraction). +# STEP3 +import gzip +import io + +def my_get_genetic_data(file_path: str) -> pd.DataFrame: + """ + Reads a GEO series matrix file (whether gzipped or plain) by ignoring lines starting with '!' + and then parsing the remainder as tab-delimited data. + Renames 'ID_REF' to 'ID' if present and sets it as the index. + """ + # Determine if the file is gzipped by checking the first two bytes + with open(file_path, 'rb') as f: + magic = f.read(2) + is_gzipped = (magic == b'\x1f\x8b') + + # Open the file accordingly + if is_gzipped: + fhandle = gzip.open(file_path, 'rt') + else: + fhandle = open(file_path, 'r') + + # Read all lines except those starting with '!' + with fhandle: + content = [line for line in fhandle if not line.startswith('!')] + + # Parse the remaining content as tab-delimited + df = pd.read_csv(io.StringIO(''.join(content)), sep='\t', comment='!', on_bad_lines='skip') + + # Rename 'ID_REF' to 'ID' if present and set as index + if 'ID_REF' in df.columns: + df = df.rename(columns={'ID_REF': 'ID'}).astype({'ID': 'str'}) + df.set_index('ID', inplace=True) + + return df + +# Use the custom function to attempt reading the gene expression data +gene_data = my_get_genetic_data(matrix_file) + +# Print the first 20 row IDs to check if we obtained any data +print(gene_data.index[:20]) \ No newline at end of file diff --git a/p1/preprocess/Melanoma/code/GSE148319.py b/p1/preprocess/Melanoma/code/GSE148319.py new file mode 100644 index 0000000000000000000000000000000000000000..228d38290a6f3e60007dc40517c63b3a8e211899 --- /dev/null +++ b/p1/preprocess/Melanoma/code/GSE148319.py @@ -0,0 +1,160 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Melanoma" +cohort = "GSE148319" + +# Input paths +in_trait_dir = "../DATA/GEO/Melanoma" +in_cohort_dir = "../DATA/GEO/Melanoma/GSE148319" + +# Output paths +out_data_file = "./output/preprocess/1/Melanoma/GSE148319.csv" +out_gene_data_file = "./output/preprocess/1/Melanoma/gene_data/GSE148319.csv" +out_clinical_data_file = "./output/preprocess/1/Melanoma/clinical_data/GSE148319.csv" +json_path = "./output/preprocess/1/Melanoma/cohort_info.json" + +# STEP1 +from tools.preprocess import * + +# 1. Attempt to identify the paths to the SOFT file and the matrix file +try: + soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) +except AssertionError: + print("[WARNING] Could not find the expected '.soft' or '.matrix' files in the directory.") + soft_file, matrix_file = None, None + +if soft_file is None or matrix_file is None: + print("[ERROR] Required GEO files are missing. Please check file names in the cohort directory.") +else: + # 2. Read the matrix file to obtain background information and sample characteristics data + background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] + clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] + background_info, clinical_data = get_background_and_clinical_data(matrix_file, + background_prefixes, + clinical_prefixes) + + # 3. Obtain the sample characteristics dictionary from the clinical dataframe + sample_characteristics_dict = get_unique_values_by_row(clinical_data) + + # 4. Explicitly print out all the background information and the sample characteristics dictionary + print("Background Information:") + print(background_info) + print("\nSample Characteristics Dictionary:") + print(sample_characteristics_dict) +# 1) Gene Expression Data Availability +is_gene_available = True # Based on the overview, this dataset appears to measure gene expression data. + +# 2) Variable Availability and Data Type Conversion +# From the sample characteristics, row 3 contains "biological replicate" info, including "hs.Melanoma.biolrep.x". +# We can interpret it as indicating whether a sample is melanoma (1) or not (0). + +trait_row = 3 +age_row = None # No 'age' info found +gender_row = None # No 'gender' info found + +def convert_trait(value: str) -> Optional[int]: + if not isinstance(value, str): + return None + # Split at the colon, take the second part + parts = value.split(":") + if len(parts) < 2: + return None + val = parts[1].strip().lower() + # Mark as 1 if 'melanoma' is in the string, else 0 + return 1 if "melanoma" in val else 0 + +# No conversion needed for age and gender as they are not available +convert_age = None +convert_gender = None + +# 3) Save Metadata: initial filtering +is_trait_available = trait_row is not None +validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4) Clinical Feature Extraction +if trait_row is not None: + selected_clinical_df = geo_select_clinical_features( + clinical_data, # assume 'clinical_data' DataFrame is provided + trait=trait, # "Melanoma" + trait_row=trait_row, + convert_trait=convert_trait, + age_row=age_row, + convert_age=convert_age, + gender_row=gender_row, + convert_gender=convert_gender + ) + preview = preview_df(selected_clinical_df) + print("Preview of selected clinical features:", preview) + selected_clinical_df.to_csv(out_clinical_data_file, index=False) +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# The provided identifiers (e.g., "11715100_at") appear to be Affymetrix probe set IDs. +# They are not human gene symbols and require mapping to gene symbols. +print("requires_gene_mapping = True") +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP: Gene Identifier Mapping + +# 1) Identify which columns store the probe identifiers and the gene symbols: +# From the annotation preview, "ID" matches the probe identifiers in gene_data.index, +# and "Gene Symbol" holds the actual human gene symbols. + +# 2) Extract the "ID" and "Gene Symbol" columns to create a mapping DataFrame +mapping_df = get_gene_mapping(gene_annotation, prob_col="ID", gene_col="Gene Symbol") + +# 3) Apply the gene mapping to convert probe-level data into gene-level data +gene_data = apply_gene_mapping(gene_data, mapping_df) +# STEP7 + +import pandas as pd + +# 1) Normalize the gene symbols and save the result +normalized_gene_data = normalize_gene_symbols_in_index(gene_data) +normalized_gene_data.to_csv(out_gene_data_file) + +# 2) Read the single-row clinical data saved in step 2, then set its row index to the trait name +# so that geo_link_clinical_genetic_data can correctly place "Melanoma" as a column. +selected_clinical_df = pd.read_csv(out_clinical_data_file, header=0) +selected_clinical_df.index = [trait] + +# 3) Link the clinical and genetic data +linked_data = geo_link_clinical_genetic_data(selected_clinical_df, normalized_gene_data) + +# 4) Handle missing values +processed_data = handle_missing_values(df=linked_data, trait_col=trait) + +# 5) Check for trait bias (and remove age/gender if present and biased) +is_trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + +# 6) Final validation and metadata saving +is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=is_trait_biased, + df=processed_data, + note="Preprocessing completed. Trait data present and gene data mapped." +) + +# 7) Save the final linked data if usable +if is_usable: + processed_data.to_csv(out_data_file, index=False) \ No newline at end of file diff --git a/p1/preprocess/Melanoma/code/GSE148949.py b/p1/preprocess/Melanoma/code/GSE148949.py new file mode 100644 index 0000000000000000000000000000000000000000..add05837e601050eaf417a48e301f260fe28be5f --- /dev/null +++ b/p1/preprocess/Melanoma/code/GSE148949.py @@ -0,0 +1,144 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Melanoma" +cohort = "GSE148949" + +# Input paths +in_trait_dir = "../DATA/GEO/Melanoma" +in_cohort_dir = "../DATA/GEO/Melanoma/GSE148949" + +# Output paths +out_data_file = "./output/preprocess/1/Melanoma/GSE148949.csv" +out_gene_data_file = "./output/preprocess/1/Melanoma/gene_data/GSE148949.csv" +out_clinical_data_file = "./output/preprocess/1/Melanoma/clinical_data/GSE148949.csv" +json_path = "./output/preprocess/1/Melanoma/cohort_info.json" + +# STEP1 +from tools.preprocess import * + +# 1. Attempt to identify the paths to the SOFT file and the matrix file +try: + soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) +except AssertionError: + print("[WARNING] Could not find the expected '.soft' or '.matrix' files in the directory.") + soft_file, matrix_file = None, None + +if soft_file is None or matrix_file is None: + print("[ERROR] Required GEO files are missing. Please check file names in the cohort directory.") +else: + # 2. Read the matrix file to obtain background information and sample characteristics data + background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] + clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] + background_info, clinical_data = get_background_and_clinical_data(matrix_file, + background_prefixes, + clinical_prefixes) + + # 3. Obtain the sample characteristics dictionary from the clinical dataframe + sample_characteristics_dict = get_unique_values_by_row(clinical_data) + + # 4. Explicitly print out all the background information and the sample characteristics dictionary + print("Background Information:") + print(background_info) + print("\nSample Characteristics Dictionary:") + print(sample_characteristics_dict) +# 1) Decide if this dataset contains gene expression data +is_gene_available = True # Based on "whole human genome Agilent arrays" info + +# 2) Determine availability for trait, age, and gender +trait_row = None # No row with distinct "Melanoma" variation found +age_row = None # No row indicating age +gender_row = None # No row indicating gender + +# 2.2) Define data conversion functions +def convert_trait(value: str) -> Optional[int]: + """ + Convert the trait value to our chosen data type. + Since trait data is unavailable, we'll return None. + """ + return None + +def convert_age(value: str) -> Optional[float]: + """ + Convert the age value to a continuous type. + Since age data is unavailable, we'll return None. + """ + return None + +def convert_gender(value: str) -> Optional[int]: + """ + Convert the gender value to binary (female=0, male=1). + Since gender data is unavailable, we'll return None. + """ + return None + +# 3) Conduct initial filtering and save metadata +is_trait_available = (trait_row is not None) +validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4) Since trait_row is None, we skip clinical feature extraction +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +print("requires_gene_mapping = True") +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP: Gene Identifier Mapping + +# 1. From the preview, the 'ID' column in gene_annotation matches the identifier type +# seen in gene_data, and the 'ORF' column stores the gene symbols. + +# 2. Build the mapping dataframe, using 'ID' as the probe column and 'ORF' as the gene symbol column. +mapping_df = get_gene_mapping(gene_annotation, prob_col='ID', gene_col='ORF') + +# 3. Convert the probe-level data in 'gene_data' to gene-level data by applying the mapping. +gene_data = apply_gene_mapping(gene_data, mapping_df) + +# (Optional) Print some information about the resulting gene data +print("Mapped gene_data shape:", gene_data.shape) +print("First 10 genes after mapping:", gene_data.index[:10].tolist()) +# STEP5 + +# 1) Normalize the gene symbols +normalized_gene_data = normalize_gene_symbols_in_index(gene_data) +normalized_gene_data.to_csv(out_gene_data_file) + +# 2) Because we found no trait data (trait_row=None), there is no clinical dataframe to link. +# We skip linking clinical data and related steps. + +# 3) We also skip handling missing trait/covariate values, since no trait is present. + +# 4) We skip bias checking on the trait for the same reason (no trait). + +# 5) Perform final quality validation and save metadata. +# Although we have gene data, the trait is unavailable, so this dataset is not usable for trait-gene association. +is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, # No trait data + is_biased=False, # Arbitrarily False; lack of trait data makes is_usable=False anyway + df=normalized_gene_data, + note="No trait data available for cohort GSE200904; gene data alone cannot support association analysis." +) + +# 6) Since the dataset is not usable (no trait), we do not save a final linked data file. +if is_usable: + # This block won't run because is_usable is False. + linked_data_final.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Melanoma/code/GSE157738.py b/p1/preprocess/Melanoma/code/GSE157738.py new file mode 100644 index 0000000000000000000000000000000000000000..f2ca755e375e73b17fda5e868dd139dbf8edff7a --- /dev/null +++ b/p1/preprocess/Melanoma/code/GSE157738.py @@ -0,0 +1,166 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Melanoma" +cohort = "GSE157738" + +# Input paths +in_trait_dir = "../DATA/GEO/Melanoma" +in_cohort_dir = "../DATA/GEO/Melanoma/GSE157738" + +# Output paths +out_data_file = "./output/preprocess/1/Melanoma/GSE157738.csv" +out_gene_data_file = "./output/preprocess/1/Melanoma/gene_data/GSE157738.csv" +out_clinical_data_file = "./output/preprocess/1/Melanoma/clinical_data/GSE157738.csv" +json_path = "./output/preprocess/1/Melanoma/cohort_info.json" + +# STEP1 +from tools.preprocess import * + +# 1. Attempt to identify the paths to the SOFT file and the matrix file +try: + soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) +except AssertionError: + print("[WARNING] Could not find the expected '.soft' or '.matrix' files in the directory.") + soft_file, matrix_file = None, None + +if soft_file is None or matrix_file is None: + print("[ERROR] Required GEO files are missing. Please check file names in the cohort directory.") +else: + # 2. Read the matrix file to obtain background information and sample characteristics data + background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] + clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] + background_info, clinical_data = get_background_and_clinical_data(matrix_file, + background_prefixes, + clinical_prefixes) + + # 3. Obtain the sample characteristics dictionary from the clinical dataframe + sample_characteristics_dict = get_unique_values_by_row(clinical_data) + + # 4. Explicitly print out all the background information and the sample characteristics dictionary + print("Background Information:") + print(background_info) + print("\nSample Characteristics Dictionary:") + print(sample_characteristics_dict) +# 1. Determine if gene expression data is available +# From the background info, data is from "Affymetrix Human Gene 2.0 ST Array," indicating gene expression data is present. +is_gene_available = True + +# 2. Check availability for 'trait' (Melanoma), 'age', and 'gender' +# According to the sample characteristics, 'patient diagnosis: melanoma' is the same for all patients => no variation. +# There's no information for age or gender. Therefore, we set them to None. +trait_row = None +age_row = None +gender_row = None + +# 2.2 Define conversion functions. +def convert_trait(value: str) -> int: + """ + For demonstration, returns None because trait data row is not available in this dataset. + """ + return None + +def convert_age(value: str) -> float: + """ + Returns None because age info is not available in this dataset. + """ + return None + +def convert_gender(value: str) -> int: + """ + Returns None because gender info is not available in this dataset. + """ + return None + +# 3. Save metadata (initial filtering) +# Trait availability is False because trait_row = None. +is_trait_available = (trait_row is not None) + +is_usable = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4. Clinical Feature Extraction +# Since trait_row is None, we do not extract or save clinical data. +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# According to the provided indices (e.g., '16650001', '16650003', etc.), they do not look like standard human gene symbols. +# They appear to be probe/array IDs that would require mapping to gene symbols. +print("requires_gene_mapping = True") +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP 6: Gene Identifier Mapping (Corrected) + +# Explanation: +# We attempted to map probes to gene symbols using the annotation dataframe, but there is no overlap +# between the row IDs in 'gene_data' and the 'ID' column in 'gene_annotation'. Consequently, the mapped +# DataFrame ended up empty. Per the reviewer's suggestion, we will detect this mismatch and, if no +# overlap is found, we will keep the original probe-level expression data in 'gene_data' (skipping the +# apply_gene_mapping step). This retains the numeric probe IDs as final identifiers. + +common_ids = gene_data.index.intersection(gene_annotation['ID'].astype(str).unique()) +if len(common_ids) == 0: + # No overlap found; use probe IDs as final identifiers + print("No overlap found between 'gene_data' row IDs and gene_annotation 'ID'.") + print("Retaining probe-level data as the final gene expression DataFrame.") + # Rename the index from "ID" to "Gene" for consistency + gene_data.index.rename("Gene", inplace=True) +else: + # If partial or full overlap is detected, proceed with actual mapping + mapping_df = get_gene_mapping( + annotation=gene_annotation, + prob_col='ID', + gene_col='GB_ACC' + ) + gene_data = apply_gene_mapping(gene_data, mapping_df) + print("Applied gene mapping. Mapped gene expression data shape:", gene_data.shape) + +# Print a quick summary +print("Final gene_data shape:", gene_data.shape) +print("Final gene_data index name:", gene_data.index.name) +print("First 5 rows:") +print(gene_data.head(5)) +# STEP5 + +# 1) Normalize the gene symbols +normalized_gene_data = normalize_gene_symbols_in_index(gene_data) +normalized_gene_data.to_csv(out_gene_data_file) + +# 2) Because we found no trait data (trait_row=None), there is no clinical dataframe to link. +# We skip linking clinical data and related steps. + +# 3) We also skip handling missing trait/covariate values, since no trait is present. + +# 4) We skip bias checking on the trait for the same reason (no trait). + +# 5) Perform final quality validation and save metadata. +# Although we have gene data, the trait is unavailable, so this dataset is not usable for trait-gene association. +is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, # No trait data + is_biased=False, # Arbitrarily False; lack of trait data makes is_usable=False anyway + df=normalized_gene_data, + note="No trait data available for cohort GSE200904; gene data alone cannot support association analysis." +) + +# 6) Since the dataset is not usable (no trait), we do not save a final linked data file. +if is_usable: + # This block won't run because is_usable is False. + linked_data_final.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Melanoma/code/GSE189631.py b/p1/preprocess/Melanoma/code/GSE189631.py new file mode 100644 index 0000000000000000000000000000000000000000..267ebef7b7ac59adbd1670a320e2ebff09c429f8 --- /dev/null +++ b/p1/preprocess/Melanoma/code/GSE189631.py @@ -0,0 +1,45 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Melanoma" +cohort = "GSE189631" + +# Input paths +in_trait_dir = "../DATA/GEO/Melanoma" +in_cohort_dir = "../DATA/GEO/Melanoma/GSE189631" + +# Output paths +out_data_file = "./output/preprocess/1/Melanoma/GSE189631.csv" +out_gene_data_file = "./output/preprocess/1/Melanoma/gene_data/GSE189631.csv" +out_clinical_data_file = "./output/preprocess/1/Melanoma/clinical_data/GSE189631.csv" +json_path = "./output/preprocess/1/Melanoma/cohort_info.json" + +# STEP1 +from tools.preprocess import * + +# 1. Attempt to identify the paths to the SOFT file and the matrix file +try: + soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) +except AssertionError: + print("[WARNING] Could not find the expected '.soft' or '.matrix' files in the directory.") + soft_file, matrix_file = None, None + +if soft_file is None or matrix_file is None: + print("[ERROR] Required GEO files are missing. Please check file names in the cohort directory.") +else: + # 2. Read the matrix file to obtain background information and sample characteristics data + background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] + clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] + background_info, clinical_data = get_background_and_clinical_data(matrix_file, + background_prefixes, + clinical_prefixes) + + # 3. Obtain the sample characteristics dictionary from the clinical dataframe + sample_characteristics_dict = get_unique_values_by_row(clinical_data) + + # 4. Explicitly print out all the background information and the sample characteristics dictionary + print("Background Information:") + print(background_info) + print("\nSample Characteristics Dictionary:") + print(sample_characteristics_dict) \ No newline at end of file diff --git a/p1/preprocess/Melanoma/code/GSE200904.py b/p1/preprocess/Melanoma/code/GSE200904.py new file mode 100644 index 0000000000000000000000000000000000000000..5067a4dd1677969c19d2bfdb3eb5682a7ec4b7d6 --- /dev/null +++ b/p1/preprocess/Melanoma/code/GSE200904.py @@ -0,0 +1,106 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Melanoma" +cohort = "GSE200904" + +# Input paths +in_trait_dir = "../DATA/GEO/Melanoma" +in_cohort_dir = "../DATA/GEO/Melanoma/GSE200904" + +# Output paths +out_data_file = "./output/preprocess/1/Melanoma/GSE200904.csv" +out_gene_data_file = "./output/preprocess/1/Melanoma/gene_data/GSE200904.csv" +out_clinical_data_file = "./output/preprocess/1/Melanoma/clinical_data/GSE200904.csv" +json_path = "./output/preprocess/1/Melanoma/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1. Gene Expression Data Availability +is_gene_available = True # This dataset measures gene expression (73 endogenous genes). + +# 2. Variable Availability +trait_row = None # No column providing melanoma vs. other states in the sample characteristics. +age_row = None # No age-related information detected. +gender_row = None # No gender-related information detected. + +# 2.2 Data Type Conversion + +def convert_trait(value: str): + # Since trait data is unavailable, return None + return None + +def convert_age(value: str): + # Since age data is unavailable, return None + return None + +def convert_gender(value: str): + # Since gender data is unavailable, return None + return None + +# 3. Initial Filtering and Metadata Saving +is_usable = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=(trait_row is not None) +) + +# 4. Clinical Feature Extraction (Skipped because 'trait_row' is None) +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# Based on the gene identifiers observed, they are standard human gene symbols. +# Therefore, gene mapping is not required. +print("requires_gene_mapping = False") +# STEP5 + +# 1) Normalize the gene symbols +normalized_gene_data = normalize_gene_symbols_in_index(gene_data) +normalized_gene_data.to_csv(out_gene_data_file) + +# 2) Because we found no trait data (trait_row=None), there is no clinical dataframe to link. +# We skip linking clinical data and related steps. + +# 3) We also skip handling missing trait/covariate values, since no trait is present. + +# 4) We skip bias checking on the trait for the same reason (no trait). + +# 5) Perform final quality validation and save metadata. +# Although we have gene data, the trait is unavailable, so this dataset is not usable for trait-gene association. +is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, # No trait data + is_biased=False, # Arbitrarily False; lack of trait data makes is_usable=False anyway + df=normalized_gene_data, + note="No trait data available for cohort GSE200904; gene data alone cannot support association analysis." +) + +# 6) Since the dataset is not usable (no trait), we do not save a final linked data file. +if is_usable: + # This block won't run because is_usable is False. + linked_data_final.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Melanoma/code/GSE202806.py b/p1/preprocess/Melanoma/code/GSE202806.py new file mode 100644 index 0000000000000000000000000000000000000000..288ed5debb5560486f6cf2bbfc2a28e1f35e0a75 --- /dev/null +++ b/p1/preprocess/Melanoma/code/GSE202806.py @@ -0,0 +1,110 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Melanoma" +cohort = "GSE202806" + +# Input paths +in_trait_dir = "../DATA/GEO/Melanoma" +in_cohort_dir = "../DATA/GEO/Melanoma/GSE202806" + +# Output paths +out_data_file = "./output/preprocess/1/Melanoma/GSE202806.csv" +out_gene_data_file = "./output/preprocess/1/Melanoma/gene_data/GSE202806.csv" +out_clinical_data_file = "./output/preprocess/1/Melanoma/clinical_data/GSE202806.csv" +json_path = "./output/preprocess/1/Melanoma/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1) Gene Expression Data Availability +is_gene_available = True # Based on the "RNA analysis of 770 genes" description + +# 2) Variable Availability and Data Type Conversion +# From the sample characteristics: +# 0: ['tissue: Melanoma'] -> single value, no variation -> treat as not available +# 1: ['nf1 status: WT', 'nf1 status: MUT'] -> not the trait "Melanoma", nor age/gender +trait_row = None # Entire dataset is Melanoma; no variation for trait +age_row = None # No age data found +gender_row = None # No gender data found + +def convert_trait(value: str) -> Optional[int]: + # This function won't be used because trait_row is None; + # but we define it as required by the instructions. + return None + +def convert_age(value: str) -> Optional[float]: + # This function won't be used because age_row is None; + # but we define it as required by the instructions. + return None + +def convert_gender(value: str) -> Optional[int]: + # This function won't be used because gender_row is None; + # but we define it as required by the instructions. + return None + +# 3) Save Metadata (initial filtering) +# Trait data is unavailable: is_trait_available=False +is_usable = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=False +) + +# 4) Clinical Feature Extraction +# Skip because trait_row is None +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +print("The listed identifiers match known human gene symbols.\nrequires_gene_mapping = False") +# STEP5 + +# 1) Normalize the gene symbols +normalized_gene_data = normalize_gene_symbols_in_index(gene_data) +normalized_gene_data.to_csv(out_gene_data_file) + +# 2) Because we found no trait data (trait_row=None), there is no clinical dataframe to link. +# We skip linking clinical data and related steps. + +# 3) We also skip handling missing trait/covariate values, since no trait is present. + +# 4) We skip bias checking on the trait for the same reason (no trait). + +# 5) Perform final quality validation and save metadata. +# Although we have gene data, the trait is unavailable, so this dataset is not usable for trait-gene association. +is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, # No trait data + is_biased=False, # Arbitrarily False; lack of trait data makes is_usable=False anyway + df=normalized_gene_data, + note="No trait data available for cohort GSE202806; gene data alone cannot support association analysis." +) + +# 6) Since the dataset is not usable (no trait), we do not save a final linked data file. +if is_usable: + # This block won't run because is_usable is False. + linked_data_final.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Melanoma/code/GSE215868.py b/p1/preprocess/Melanoma/code/GSE215868.py new file mode 100644 index 0000000000000000000000000000000000000000..795643fe8da2461c3240db40e391dc32a184609d --- /dev/null +++ b/p1/preprocess/Melanoma/code/GSE215868.py @@ -0,0 +1,119 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Melanoma" +cohort = "GSE215868" + +# Input paths +in_trait_dir = "../DATA/GEO/Melanoma" +in_cohort_dir = "../DATA/GEO/Melanoma/GSE215868" + +# Output paths +out_data_file = "./output/preprocess/1/Melanoma/GSE215868.csv" +out_gene_data_file = "./output/preprocess/1/Melanoma/gene_data/GSE215868.csv" +out_clinical_data_file = "./output/preprocess/1/Melanoma/clinical_data/GSE215868.csv" +json_path = "./output/preprocess/1/Melanoma/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# Step 1: Determine gene expression data availability +is_gene_available = True # Based on "Baseline gene expression profiling..." in the background + +# Step 2.1: Determine data availability +# The entire dataset is advanced melanoma patients, so there's no variation for the "trait". +# Hence, trait_row is None. +trait_row = None + +# Age information is found at key 0 +age_row = 0 + +# No information about gender is found +gender_row = None + +# Step 2.2: Define conversion functions +def convert_trait(x: str) -> int: + # Not used in this dataset since trait_row is None, but provided for completeness + # If it were used, parse the value after the colon and map to 0/1 or None for unknown + val = x.split(':')[-1].strip().lower() + # Example: if val == "melanoma": return 1 + return None + +def convert_age(x: str) -> float: + # Parse out the integer after "age:" + parts = x.split(':') + if len(parts) < 2: + return None + value = parts[1].strip() + if value.isdigit(): + return float(value) + return None + +def convert_gender(x: str) -> int: + # Not used (gender_row is None), but provided for completeness + val = x.split(':')[-1].strip().lower() + if val in ["male", "m"]: + return 1 + elif val in ["female", "f"]: + return 0 + return None + +# Step 3: Save metadata with initial filtering +# trait_row is None => trait not available +is_trait_available = (trait_row is not None) +is_usable = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# Step 4: Skip clinical feature extraction because trait_row is None +# (No code for geo_select_clinical_features is needed here since trait data is not available). +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# After observing the provided gene identifiers (A2M, ABCF1, ACVR1C, etc.), they match known official human gene symbols. +# Therefore, no additional gene symbol mapping is required. + +print("requires_gene_mapping = False") +# STEP 5 + +# 1. Normalize the obtained gene expression data using the NCBI Gene synonym database +normalized_gene_data = normalize_gene_symbols_in_index(gene_data) +normalized_gene_data.to_csv(out_gene_data_file) + +# The dataset lacks trait data (trait_row is None), so we cannot proceed with linking or final validation. +# Instead, we perform an initial-style validation to record the dataset as not usable for association analysis. + +is_usable = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=True, # Gene data is indeed available + is_trait_available=False, # Trait is absent + note="Trait data is not available. Gene expression data alone is not enough for analysis." +) + +# Since trait data is unavailable, we skip linking clinical data, missing value handling, or final validation. +# No further action is needed, as the dataset is determined unusable for trait-based studies. \ No newline at end of file diff --git a/p1/preprocess/Melanoma/code/GSE244984.py b/p1/preprocess/Melanoma/code/GSE244984.py new file mode 100644 index 0000000000000000000000000000000000000000..59e8d266fcd48754db47937e1821079c5fde37ea --- /dev/null +++ b/p1/preprocess/Melanoma/code/GSE244984.py @@ -0,0 +1,164 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Melanoma" +cohort = "GSE244984" + +# Input paths +in_trait_dir = "../DATA/GEO/Melanoma" +in_cohort_dir = "../DATA/GEO/Melanoma/GSE244984" + +# Output paths +out_data_file = "./output/preprocess/1/Melanoma/GSE244984.csv" +out_gene_data_file = "./output/preprocess/1/Melanoma/gene_data/GSE244984.csv" +out_clinical_data_file = "./output/preprocess/1/Melanoma/clinical_data/GSE244984.csv" +json_path = "./output/preprocess/1/Melanoma/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1) Gene Expression Data Availability +is_gene_available = True # Based on background ("Molecular patterns ..."), we assume there's gene expression data. + +# 2) Variable Availability and Data Type Conversion + +# After checking the sample characteristics dictionary, +# we see only: +# 0 => ["patient: Pat..."] +# 1 => ["resistance: CTLA4res", "resistance: PD1res"] +# There's no mention of "Melanoma" (the trait), age, or gender. +# Also, the entire cohort is already known to be melanoma, so it's effectively a constant in this dataset. +# Therefore, these variables are considered "not available" for analysis. +trait_row = None +age_row = None +gender_row = None + +# Define the conversion functions. Though we have no data rows for them, +# we must still define them to fulfill the instructions. + +def convert_trait(x: str): + """ + Convert trait data to a desired format (if it existed). + Extract the substring after the colon, handle unknowns as None. + """ + parts = x.split(':') + if len(parts) < 2: + return None + val = parts[1].strip().lower() + # Hypothetical conversion if available + # But in this case, there's no trait data row anyway + return None # Because we don't have actual trait info + +def convert_age(x: str): + """ + Convert age data to continuous numeric (if it existed). + Extract the substring after the colon, handle unknowns as None. + """ + parts = x.split(':') + if len(parts) < 2: + return None + val = parts[1].strip() + # We don't have an age row. Return None for demonstration. + return None + +def convert_gender(x: str): + """ + Convert gender data to binary (0=female, 1=male) if it existed. + Extract the substring after the colon, handle unknowns as None. + """ + parts = x.split(':') + if len(parts) < 2: + return None + val = parts[1].strip().lower() + # We don't have a gender row. Return None for demonstration. + return None + +# 3) Save Metadata with initial filtering +# Trait data availability is determined by whether trait_row is None. +is_trait_available = (trait_row is not None) + +# Record dataset usability info +is_usable_initial = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4) Clinical Feature Extraction +# Because trait_row is None, we skip feature extraction entirely. +# (No additional steps are needed.) +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# The observed identifiers are recognized human gene symbols. +print("requires_gene_mapping = False") +# STEP 5 + +# 1) Normalize the obtained gene data using the NCBI Gene synonym database +normalized_gene_data = normalize_gene_symbols_in_index(gene_data) +normalized_gene_data.to_csv(out_gene_data_file) + +# 2) Check whether trait data is available (set in a previous step). +# If trait data is not available, we skip linking and subsequent steps +# because there's no clinical data to merge with gene data for association analysis. +if not is_trait_available: + # Provide a dummy DataFrame and a placeholder boolean (e.g., False for is_biased) + # to satisfy the function signature for final validation. + import pandas as pd + dummy_df = pd.DataFrame() + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=False, + df=dummy_df, + note="Trait not available, skipping linking step." + ) +else: + # If the trait is available, link clinical and genetic data + linked_data = geo_link_clinical_genetic_data(selected_clinical_df, normalized_gene_data) + + # 3) Handle missing values using trait as the key + linked_data_processed = handle_missing_values(linked_data, trait_col=trait) + + # 4) Assess bias and remove biased demographic features + trait_biased, linked_data_final = judge_and_remove_biased_features(linked_data_processed, trait) + + # 5) Final validation and metadata saving + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=linked_data_final, + note="Dataset processed with GEO pipeline. Checked for missing values and bias." + ) + + # 6) Save final linked data if usable + if is_usable: + linked_data_final.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Melanoma/code/GSE261347.py b/p1/preprocess/Melanoma/code/GSE261347.py new file mode 100644 index 0000000000000000000000000000000000000000..8b52c2f9b25aaef1bdd78f71a0fb124041a6c43a --- /dev/null +++ b/p1/preprocess/Melanoma/code/GSE261347.py @@ -0,0 +1,114 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Melanoma" +cohort = "GSE261347" + +# Input paths +in_trait_dir = "../DATA/GEO/Melanoma" +in_cohort_dir = "../DATA/GEO/Melanoma/GSE261347" + +# Output paths +out_data_file = "./output/preprocess/1/Melanoma/GSE261347.csv" +out_gene_data_file = "./output/preprocess/1/Melanoma/gene_data/GSE261347.csv" +out_clinical_data_file = "./output/preprocess/1/Melanoma/clinical_data/GSE261347.csv" +json_path = "./output/preprocess/1/Melanoma/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1. Determine gene expression availability +is_gene_available = True # The dataset contains transcriptomic profiling data. + +# 2. Identify data availability and define conversion functions +# No trait/age/gender keys found; set their row to None. +trait_row = None +age_row = None +gender_row = None + +# Define data type conversion functions. +# Even though data is not available, we still provide these for code completeness. + +def convert_trait(value: str): + # Example parse: get substring after colon + val = value.split(':')[-1].strip().lower() + # If data were available, map recognized trait values to 1, else None + if "melanoma" in val: + return 1 + return None + +def convert_age(value: str): + # Example parse: get substring after colon + val = value.split(':')[-1].strip().lower() + # Convert to float if possible, else None + try: + return float(val) + except ValueError: + return None + +def convert_gender(value: str): + # Example parse: get substring after colon + val = value.split(':')[-1].strip().lower() + if val in ["male", "m"]: + return 1 + elif val in ["female", "f"]: + return 0 + return None + +# 3. Perform initial filtering and save metadata +# Trait availability is false because trait_row is None. +is_trait_available = (trait_row is not None) + +validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4. Clinical feature extraction +# Skip because trait_row is None (trait not available). +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# After reviewing the gene identifiers, they appear to be standard human gene symbols. +print("requires_gene_mapping = False") +# STEP5 +# Since trait data is not available, we cannot perform a final validation (is_final=True) because +# 'df' and 'is_biased' are required. Instead, we will perform a partial validation (is_final=False) +# to record that the dataset lacks trait data. + +# 1. Normalize the obtained gene data using the NCBI Gene synonym database. +normalized_gene_data = normalize_gene_symbols_in_index(gene_data) +normalized_gene_data.to_csv(out_gene_data_file) + +# 2. Perform partial validation and save metadata that trait data is not available. +validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=True, # We do have transcriptomic data + is_trait_available=False # Trait data remains unavailable +) + +# 3. Since trait data is not available, we cannot link or bias-check, so we stop here. \ No newline at end of file diff --git a/p1/preprocess/Melanoma/code/TCGA.py b/p1/preprocess/Melanoma/code/TCGA.py new file mode 100644 index 0000000000000000000000000000000000000000..aa2e07fb6f9d5cd64e8e32932bf8b2d878f4212e --- /dev/null +++ b/p1/preprocess/Melanoma/code/TCGA.py @@ -0,0 +1,116 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Melanoma" + +# Input paths +tcga_root_dir = "../DATA/TCGA" + +# Output paths +out_data_file = "./output/preprocess/1/Melanoma/TCGA.csv" +out_gene_data_file = "./output/preprocess/1/Melanoma/gene_data/TCGA.csv" +out_clinical_data_file = "./output/preprocess/1/Melanoma/clinical_data/TCGA.csv" +json_path = "./output/preprocess/1/Melanoma/cohort_info.json" + +import os +import pandas as pd + +# Step 1: Search directories in tcga_root_dir for "most specific" matches to "Melanoma". +# We define a simple priority-based approach: "skcm" is highest priority, then "melanoma", then "skin". +search_terms_priority = { + "skcm": 0, + "melanoma": 1, + "skin": 2 +} + +dir_list = os.listdir(tcga_root_dir) +best_dir = None +best_rank = None + +for d in dir_list: + d_lower = d.lower() + # Determine if this directory name contains any search term + matched_terms = [term for term in search_terms_priority if term in d_lower] + if matched_terms: + # Pick the highest priority (lowest numerical value) + rank = min(search_terms_priority[t] for t in matched_terms) + if best_rank is None or rank < best_rank: + best_rank = rank + best_dir = d + +if best_dir is None: + # No matching directory found. Mark the dataset as skipped. + validate_and_save_cohort_info( + is_final=False, + cohort="TCGA", + info_path=json_path, + is_gene_available=False, + is_trait_available=False + ) +else: + # 2. Identify the clinicalMatrix and PANCAN files + cohort_dir = os.path.join(tcga_root_dir, best_dir) + clinical_file_path, genetic_file_path = tcga_get_relevant_filepaths(cohort_dir) + + # 3. Load both data files + clinical_df = pd.read_csv(clinical_file_path, index_col=0, sep='\t') + genetic_df = pd.read_csv(genetic_file_path, index_col=0, sep='\t') + + # 4. Print the column names of the clinical data + print("Clinical Data Columns:") + print(clinical_df.columns.tolist()) +candidate_age_cols = ["age_at_initial_pathologic_diagnosis", "days_to_birth"] +candidate_gender_cols = ["gender"] + +extracted_columns = candidate_age_cols + candidate_gender_cols +extracted_data = clinical_df[extracted_columns] +print("candidate_age_cols =", candidate_age_cols) +print("candidate_gender_cols =", candidate_gender_cols) +print(preview_df(extracted_data, n=5)) +age_col = "age_at_initial_pathologic_diagnosis" +gender_col = "gender" + +print("The selected age_col is:", age_col) +print("The selected gender_col is:", gender_col) +# 1) Extract and standardize clinical features (trait, age, gender) from the TCGA data +selected_clinical_df = tcga_select_clinical_features( + clinical_df=clinical_df, + trait=trait, + age_col=age_col, + gender_col=gender_col +) + +# 2) Normalize gene symbols in the gene expression data +genetic_df_normalized = normalize_gene_symbols_in_index(genetic_df) +genetic_df_normalized.to_csv(out_gene_data_file) + +# 3) Link clinical and genetic data on sample IDs +gene_expr_t = genetic_df_normalized.T +linked_data = selected_clinical_df.join(gene_expr_t, how='inner') + +# 4) Handle missing values in the linked data +linked_data = handle_missing_values(linked_data, trait) + +# 5) Determine whether the trait and some demographic features are severely biased +trait_biased, linked_data = judge_and_remove_biased_features(linked_data, trait) + +# 6) Validate and save cohort information +is_usable = validate_and_save_cohort_info( + is_final=True, + cohort="TCGA", + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=linked_data, + note="Prostate Cancer data from TCGA." +) + +# 7) If usable, save the final linked data, including clinical and genetic features +if is_usable: + linked_data.to_csv(out_data_file) + # Save clinical subset if present + clinical_cols = [col for col in [trait, "Age", "Gender"] if col in linked_data.columns] + if clinical_cols: + linked_data[clinical_cols].to_csv(out_clinical_data_file) \ No newline at end of file diff --git a/p1/preprocess/Melanoma/gene_data/GSE148319.csv b/p1/preprocess/Melanoma/gene_data/GSE148319.csv new file mode 100644 index 0000000000000000000000000000000000000000..1c8b142463001f3f2372f796f1754103413c88d6 --- /dev/null +++ b/p1/preprocess/Melanoma/gene_data/GSE148319.csv @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:c5c3b4180c7aa66e8d770c8ee26682757cbfbf074099dab8eb980a72b0c013cf +size 22245412 diff --git a/p1/preprocess/Melanoma/gene_data/GSE148949.csv b/p1/preprocess/Melanoma/gene_data/GSE148949.csv new file mode 100644 index 0000000000000000000000000000000000000000..94084002e74e391d3a1d8fb35a9ea2a443192ec7 --- /dev/null +++ b/p1/preprocess/Melanoma/gene_data/GSE148949.csv @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:2b3b053d323f55d881c67cded230cd46ecbed8115f2109c1f264fd33992ccfe8 +size 12311824 diff --git a/p1/preprocess/Melanoma/gene_data/GSE157738.csv b/p1/preprocess/Melanoma/gene_data/GSE157738.csv new file mode 100644 index 0000000000000000000000000000000000000000..b1b3171dcd541107fd6a645bc54f6cb1836e7ed3 --- /dev/null +++ b/p1/preprocess/Melanoma/gene_data/GSE157738.csv @@ -0,0 +1 @@ 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diff --git a/p1/preprocess/Mesothelioma/code/GSE107754.py b/p1/preprocess/Mesothelioma/code/GSE107754.py new file mode 100644 index 0000000000000000000000000000000000000000..18c6d1e7975e2ee3c4b5ffb192237eb5a16c6ac9 --- /dev/null +++ b/p1/preprocess/Mesothelioma/code/GSE107754.py @@ -0,0 +1,196 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Mesothelioma" +cohort = "GSE107754" + +# Input paths +in_trait_dir = "../DATA/GEO/Mesothelioma" +in_cohort_dir = "../DATA/GEO/Mesothelioma/GSE107754" + +# Output paths +out_data_file = "./output/preprocess/1/Mesothelioma/GSE107754.csv" +out_gene_data_file = "./output/preprocess/1/Mesothelioma/gene_data/GSE107754.csv" +out_clinical_data_file = "./output/preprocess/1/Mesothelioma/clinical_data/GSE107754.csv" +json_path = "./output/preprocess/1/Mesothelioma/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1) Gene Expression Data Availability +# According to the series summary, this dataset uses whole human genome microarrays, so it likely contains gene expression data. +is_gene_available = True + +# 2) Variable Availability +# From the sample characteristics dictionary: +# - trait (Mesothelioma) can be identified in row 2 via "tissue: Malignant Mesothelioma" among multiple tissue types. +# - age data is not provided in any row. +# - gender data is provided in row 0 ("gender: Male"/"gender: Female"). +trait_row = 2 +age_row = None +gender_row = 0 + +# 2.2 Data Type Conversion Functions + +def convert_trait(x: str): + """ + Convert the tissue type into a binary indicator for Mesothelioma vs non-Mesothelioma. + """ + parts = x.split(':', 1) + if len(parts) < 2: + return None + value = parts[1].strip().lower() + return 1 if value == 'malignant mesothelioma' else 0 + +def convert_age(x: str): + """ + Age is not actually available (age_row=None), but define a placeholder if needed. + """ + return None + +def convert_gender(x: str): + """ + Convert gender strings to binary: Female -> 0, Male -> 1. + """ + parts = x.split(':', 1) + if len(parts) < 2: + return None + value = parts[1].strip().lower() + if value == 'female': + return 0 + elif value == 'male': + return 1 + return None + +# 3) Save Metadata (initial filtering) +is_trait_available = (trait_row is not None) +_ = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4) Clinical Feature Extraction +# Only proceed if trait_row is not None. +if trait_row is not None: + selected_clinical_df = geo_select_clinical_features( + clinical_data, + trait=trait, + trait_row=trait_row, + convert_trait=convert_trait, + age_row=age_row, + convert_age=convert_age, + gender_row=gender_row, + convert_gender=convert_gender + ) + # Preview and save + print(preview_df(selected_clinical_df)) + selected_clinical_df.to_csv(out_clinical_data_file, index=False) +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# Based on common GEO microarray conventions, these IDs (e.g., A_23_P100001) appear to be probe identifiers rather than human gene symbols. +# Therefore, they require mapping to gene symbols. + +print("requires_gene_mapping = True") +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP 6: Gene Identifier Mapping +# 1) Identify the matching columns in the annotation DataFrame: +# - 'ID' holds the same probe identifiers as gene_data.index +# - 'GENE_SYMBOL' stores the gene symbols +mapping_df = get_gene_mapping(gene_annotation, prob_col='ID', gene_col='GENE_SYMBOL') + +# 2) Convert probe-level measurements to gene-level data +gene_data = apply_gene_mapping(gene_data, mapping_df) + +# Optional preview of the resulting gene_data +print("Mapped gene expression data preview:") +print(preview_df(gene_data)) + +# Save the mapped gene data +gene_data.to_csv(out_gene_data_file, index=True) +import os +import pandas as pd + +# STEP 7: Data Normalization and Linking + +# First, check if the clinical CSV file exists. If it does not, we cannot proceed with trait-based linking. +if not os.path.exists(out_clinical_data_file): + # No trait data file => dataset is not usable for trait analysis + df_null = pd.DataFrame() + is_biased = True # Arbitrary boolean to satisfy function requirement + validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=is_biased, + df=df_null, + note="No trait data file found; dataset not usable for trait analysis." + ) + +else: + # 1. Normalize the mapped gene expression data using known gene symbol synonyms, then save. + normalized_gene_data = normalize_gene_symbols_in_index(gene_data) + normalized_gene_data.to_csv(out_gene_data_file) + + # 2. Load the previously extracted clinical CSV. + selected_clinical_df = pd.read_csv(out_clinical_data_file) + # If we had a single-row trait, rename row 0 to the trait name (example usage). + selected_clinical_df = selected_clinical_df.rename(index={0: trait}) + + # Combine these as our final clinical data; in this dataset, we only have trait info (if any). + combined_clinical_df = selected_clinical_df + + # Link the clinical and genetic data by matching sample IDs in columns. + linked_data = geo_link_clinical_genetic_data(combined_clinical_df, normalized_gene_data) + + # 3. Handle missing values in the linked data (drop incomplete rows/columns, then impute). + processed_data = handle_missing_values(linked_data, trait) + + # 4. Check trait bias and remove any biased demographic features (if any). + trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + + # 5. Final validation and metadata saving. + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=processed_data, + note="Completed trait-based preprocessing." + ) + + # 6. If final dataset is usable, save. Otherwise, skip. + if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Mesothelioma/code/GSE112154.py b/p1/preprocess/Mesothelioma/code/GSE112154.py new file mode 100644 index 0000000000000000000000000000000000000000..f7ff78602a2afadab3b84aeba350a8a8f023cefd --- /dev/null +++ b/p1/preprocess/Mesothelioma/code/GSE112154.py @@ -0,0 +1,183 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Mesothelioma" +cohort = "GSE112154" + +# Input paths +in_trait_dir = "../DATA/GEO/Mesothelioma" +in_cohort_dir = "../DATA/GEO/Mesothelioma/GSE112154" + +# Output paths +out_data_file = "./output/preprocess/1/Mesothelioma/GSE112154.csv" +out_gene_data_file = "./output/preprocess/1/Mesothelioma/gene_data/GSE112154.csv" +out_clinical_data_file = "./output/preprocess/1/Mesothelioma/clinical_data/GSE112154.csv" +json_path = "./output/preprocess/1/Mesothelioma/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1) Decide if gene expression data is available +is_gene_available = True # Based on the series summary stating "Gene expression profiling..." + +# 2) Identify availability and rows for trait, age, gender +# From the sample characteristics dictionary, we only have key 0 with multiple values: +# ['sample type: normal peritoneum', 'sample type: DMPM cell line', 'sample type: DMPM frozen tumor specimen'] +# We will use this key to derive the disease trait, which can be mapped to binary Mesothelioma vs. normal. +trait_row = 0 +age_row = None # No age info +gender_row = None # No gender info + +# 2) Data type conversion functions +def convert_trait(value: str): + # Extract substring after the colon + if ':' in value: + value = value.split(':', 1)[1].strip().lower() + else: + value = value.strip().lower() + # Binary encoding: normal -> 0, DMPM (mesothelioma) -> 1 + if 'normal' in value: + return 0 + elif 'dmpm' in value: + return 1 + return None + +def convert_age(value: str): + # No data, just return None + return None + +def convert_gender(value: str): + # No data, just return None + return None + +# 3) Conduct initial filtering and save metadata +is_trait_available = (trait_row is not None) +is_usable = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4) Extract clinical features if trait_row is available +if trait_row is not None: + selected_clinical_df = geo_select_clinical_features( + clinical_df=clinical_data, + trait=trait, + trait_row=trait_row, + convert_trait=convert_trait, + age_row=age_row, + convert_age=convert_age, + gender_row=gender_row, + convert_gender=convert_gender + ) + # Preview the extracted clinical features + preview_dict = preview_df(selected_clinical_df, n=5, max_items=200) + print("Preview of selected clinical features:", preview_dict) + + # Save clinical data to CSV + selected_clinical_df.to_csv(out_clinical_data_file, index=False) +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# Based on the observed identifiers (e.g., ILMN_1343291), they are Illumina probe IDs, not standard human gene symbols. +print("requires_gene_mapping = True") +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP: Gene Identifier Mapping + +# 1) Identify columns in gene_annotation that match probe IDs in gene_data and gene symbols: +# From the annotation preview, "ID" corresponds to probe IDs like "ILMN_...", and "Symbol" corresponds to gene symbols. +prob_col = "ID" +gene_col = "Symbol" + +# 2) Get a gene mapping dataframe +mapping_df = get_gene_mapping(gene_annotation, prob_col=prob_col, gene_col=gene_col) + +# 3) Convert probe-level measurements to gene expression data +gene_data = apply_gene_mapping(expression_df=gene_data, mapping_df=mapping_df) + +# (gene_data is now the mapped gene expression dataframe.) +import os +import pandas as pd + +# STEP 7: Data Normalization and Linking + +# First, check if the clinical CSV file exists. If it does not, we cannot proceed with trait-based linking. +if not os.path.exists(out_clinical_data_file): + # No trait data file => dataset is not usable for trait analysis + df_null = pd.DataFrame() + is_biased = True # Arbitrary boolean to satisfy function requirement + validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=is_biased, + df=df_null, + note="No trait data file found; dataset not usable for trait analysis." + ) + +else: + # 1. Normalize the mapped gene expression data using known gene symbol synonyms, then save. + normalized_gene_data = normalize_gene_symbols_in_index(gene_data) + normalized_gene_data.to_csv(out_gene_data_file) + + # 2. Load the previously extracted clinical CSV. + selected_clinical_df = pd.read_csv(out_clinical_data_file) + # If we had a single-row trait, rename row 0 to the trait name (example usage). + selected_clinical_df = selected_clinical_df.rename(index={0: trait}) + + # Combine these as our final clinical data; in this dataset, we only have trait info (if any). + combined_clinical_df = selected_clinical_df + + # Link the clinical and genetic data by matching sample IDs in columns. + linked_data = geo_link_clinical_genetic_data(combined_clinical_df, normalized_gene_data) + + # 3. Handle missing values in the linked data (drop incomplete rows/columns, then impute). + processed_data = handle_missing_values(linked_data, trait) + + # 4. Check trait bias and remove any biased demographic features (if any). + trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + + # 5. Final validation and metadata saving. + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=processed_data, + note="Completed trait-based preprocessing." + ) + + # 6. If final dataset is usable, save. Otherwise, skip. + if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Mesothelioma/code/GSE117668.py b/p1/preprocess/Mesothelioma/code/GSE117668.py new file mode 100644 index 0000000000000000000000000000000000000000..785fdac3477bf21b392862a4c7b98b3877c1a5ac --- /dev/null +++ b/p1/preprocess/Mesothelioma/code/GSE117668.py @@ -0,0 +1,182 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Mesothelioma" +cohort = "GSE117668" + +# Input paths +in_trait_dir = "../DATA/GEO/Mesothelioma" +in_cohort_dir = "../DATA/GEO/Mesothelioma/GSE117668" + +# Output paths +out_data_file = "./output/preprocess/1/Mesothelioma/GSE117668.csv" +out_gene_data_file = "./output/preprocess/1/Mesothelioma/gene_data/GSE117668.csv" +out_clinical_data_file = "./output/preprocess/1/Mesothelioma/clinical_data/GSE117668.csv" +json_path = "./output/preprocess/1/Mesothelioma/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1. Determine if the dataset likely contains gene expression data +is_gene_available = True # Based on the description, it is microarray gene expression data. + +# 2. Variable Availability and Data Type Conversion + +# From the sample characteristics: +# Row 1 => "diagnosis: healthy" or "diagnosis: malignant pleural mesothelioma" +# That can serve as the trait variable with two unique values. +trait_row = 1 +age_row = None +gender_row = None + +# Converters +def convert_trait(value: str): + """ + Convert the trait data after the colon into a binary format: + - healthy -> 0 + - malignant pleural mesothelioma -> 1 + - otherwise -> None + """ + parts = value.split(':') + val = parts[-1].strip() if len(parts) > 1 else value.strip() + val_lower = val.lower() + if 'healthy' in val_lower: + return 0 + elif 'mesothelioma' in val_lower: + return 1 + else: + return None + +# Since age and gender are not available, we'll set them to None +convert_age = None +convert_gender = None + +# 3. Initial filtering and save metadata +is_trait_available = (trait_row is not None) +validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4. Clinical Feature Extraction if trait is available +if trait_row is not None: + selected_clinical_df = geo_select_clinical_features( + clinical_df=clinical_data, + trait=trait, + trait_row=trait_row, + convert_trait=convert_trait, + age_row=age_row, + convert_age=convert_age, + gender_row=gender_row, + convert_gender=convert_gender + ) + + # Preview the resulting dataframe + preview_result = preview_df(selected_clinical_df) + print("Preview of selected clinical features:\n", preview_result) + + # Save the clinical features to CSV + selected_clinical_df.to_csv(out_clinical_data_file) +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# These identifiers (e.g., '100009613_at', '100009676_at') are Affymetrix probe IDs, not human gene symbols. +# Therefore, we need to map them to gene symbols. + +print("requires_gene_mapping = True") +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP: Gene Identifier Mapping + +# 1 & 2. Decide which columns correspond to the probe IDs and the gene symbols for mapping +# Based on the preview, "ID" holds the probe identifier (matches gene_data.index), +# and "Description" contains text describing the gene (to extract gene symbols). +mapping_df = get_gene_mapping(gene_annotation, prob_col="ID", gene_col="Description") + +# 3. Apply the mapping to convert probe-level measurements into gene-level expression data +gene_data = apply_gene_mapping(gene_data, mapping_df) +import os +import pandas as pd + +# STEP 7: Data Normalization and Linking + +# First, check if the clinical CSV file exists. If it does not, we cannot proceed with trait-based linking. +if not os.path.exists(out_clinical_data_file): + # No trait data file => dataset is not usable for trait analysis + df_null = pd.DataFrame() + is_biased = True # Arbitrary boolean to satisfy function requirement + validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=is_biased, + df=df_null, + note="No trait data file found; dataset not usable for trait analysis." + ) + +else: + # 1. Normalize the mapped gene expression data using known gene symbol synonyms, then save. + normalized_gene_data = normalize_gene_symbols_in_index(gene_data) + normalized_gene_data.to_csv(out_gene_data_file) + + # 2. Load the previously extracted clinical CSV. + selected_clinical_df = pd.read_csv(out_clinical_data_file) + # If we had a single-row trait, rename row 0 to the trait name (example usage). + selected_clinical_df = selected_clinical_df.rename(index={0: trait}) + + # Combine these as our final clinical data; in this dataset, we only have trait info (if any). + combined_clinical_df = selected_clinical_df + + # Link the clinical and genetic data by matching sample IDs in columns. + linked_data = geo_link_clinical_genetic_data(combined_clinical_df, normalized_gene_data) + + # 3. Handle missing values in the linked data (drop incomplete rows/columns, then impute). + processed_data = handle_missing_values(linked_data, trait) + + # 4. Check trait bias and remove any biased demographic features (if any). + trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + + # 5. Final validation and metadata saving. + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=processed_data, + note="Completed trait-based preprocessing." + ) + + # 6. If final dataset is usable, save. Otherwise, skip. + if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Mesothelioma/code/GSE131027.py b/p1/preprocess/Mesothelioma/code/GSE131027.py new file mode 100644 index 0000000000000000000000000000000000000000..4dbb41c4738f151edd3ba1bade65ae4f9985a66a --- /dev/null +++ b/p1/preprocess/Mesothelioma/code/GSE131027.py @@ -0,0 +1,166 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Mesothelioma" +cohort = "GSE131027" + +# Input paths +in_trait_dir = "../DATA/GEO/Mesothelioma" +in_cohort_dir = "../DATA/GEO/Mesothelioma/GSE131027" + +# Output paths +out_data_file = "./output/preprocess/1/Mesothelioma/GSE131027.csv" +out_gene_data_file = "./output/preprocess/1/Mesothelioma/gene_data/GSE131027.csv" +out_clinical_data_file = "./output/preprocess/1/Mesothelioma/clinical_data/GSE131027.csv" +json_path = "./output/preprocess/1/Mesothelioma/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1. Gene Expression Data Availability +is_gene_available = True # Based on the "investigation of expression features" context + +# 2. Variable Availability and Data Type Conversion +# We see "Mesothelioma" among multiple cancer types in key=1, so: +trait_row = 1 +age_row = None +gender_row = None + +# Define converters. For rows that are None, we will not use the converter. +def convert_trait(raw_value: str): + parts = raw_value.split(":", 1) + if len(parts) < 2: + return None + val = parts[1].strip().lower() + # Convert Mesothelioma to 1, others to 0 + return 1 if val == "mesothelioma" else 0 + +convert_age = None +convert_gender = None + +# 3. Save Metadata (Initial Filtering) +is_trait_available = (trait_row is not None) +is_usable = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4. Clinical Feature Extraction (only if trait_row is not None) +if trait_row is not None: + selected_clinical_data = geo_select_clinical_features( + clinical_df=clinical_data, + trait=trait, + trait_row=trait_row, + convert_trait=convert_trait, + age_row=age_row, + convert_age=convert_age, + gender_row=gender_row, + convert_gender=convert_gender + ) + preview_info = preview_df(selected_clinical_data) + print("Clinical data preview:", preview_info) + selected_clinical_data.to_csv(out_clinical_data_file) +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# These identifiers appear to be Affymetrix probe set IDs, not standard human gene symbols. +# Hence, gene symbol mapping will be required. + +print("requires_gene_mapping = True") +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP6 - Gene Identifier Mapping + +# 1. The key in 'gene_annotation' that corresponds to the gene expression data's identifiers is "ID". +# The key that holds the gene symbols is "Gene Symbol". + +# 2. Create a gene mapping dataframe by extracting those two columns. +mapping_df = get_gene_mapping(gene_annotation, prob_col="ID", gene_col="Gene Symbol") + +# 3. Convert probe-level measurements to gene expression data. +gene_data = apply_gene_mapping(gene_data, mapping_df) +import os +import pandas as pd + +# STEP 7: Data Normalization and Linking + +# First, check if the clinical CSV file exists. If it does not, we cannot proceed with trait-based linking. +if not os.path.exists(out_clinical_data_file): + # No trait data file => dataset is not usable for trait analysis + df_null = pd.DataFrame() + is_biased = True # Arbitrary boolean to satisfy function requirement + validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=is_biased, + df=df_null, + note="No trait data file found; dataset not usable for trait analysis." + ) + +else: + # 1. Normalize the mapped gene expression data using known gene symbol synonyms, then save. + normalized_gene_data = normalize_gene_symbols_in_index(gene_data) + normalized_gene_data.to_csv(out_gene_data_file) + + # 2. Load the previously extracted clinical CSV. + selected_clinical_df = pd.read_csv(out_clinical_data_file) + # If we had a single-row trait, rename row 0 to the trait name (example usage). + selected_clinical_df = selected_clinical_df.rename(index={0: trait}) + + # Combine these as our final clinical data; in this dataset, we only have trait info (if any). + combined_clinical_df = selected_clinical_df + + # Link the clinical and genetic data by matching sample IDs in columns. + linked_data = geo_link_clinical_genetic_data(combined_clinical_df, normalized_gene_data) + + # 3. Handle missing values in the linked data (drop incomplete rows/columns, then impute). + processed_data = handle_missing_values(linked_data, trait) + + # 4. Check trait bias and remove any biased demographic features (if any). + trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + + # 5. Final validation and metadata saving. + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=processed_data, + note="Completed trait-based preprocessing." + ) + + # 6. If final dataset is usable, save. Otherwise, skip. + if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Mesothelioma/code/GSE163720.py b/p1/preprocess/Mesothelioma/code/GSE163720.py new file mode 100644 index 0000000000000000000000000000000000000000..193ab0706aab38e8c1620117b4290c3463820357 --- /dev/null +++ b/p1/preprocess/Mesothelioma/code/GSE163720.py @@ -0,0 +1,168 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Mesothelioma" +cohort = "GSE163720" + +# Input paths +in_trait_dir = "../DATA/GEO/Mesothelioma" +in_cohort_dir = "../DATA/GEO/Mesothelioma/GSE163720" + +# Output paths +out_data_file = "./output/preprocess/1/Mesothelioma/GSE163720.csv" +out_gene_data_file = "./output/preprocess/1/Mesothelioma/gene_data/GSE163720.csv" +out_clinical_data_file = "./output/preprocess/1/Mesothelioma/clinical_data/GSE163720.csv" +json_path = "./output/preprocess/1/Mesothelioma/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1) Decide whether gene expression data is available +is_gene_available = True # Based on the background info mentioning microarray gene expression analysis + +# 2) Determine data availability and define row indices +# Check the sample characteristics dictionary: +# {0: ['organ: Tumor'], 1: ['compartment: Tissue'], 2: ['Sex: F', 'Sex: M']} +# There is no row for the trait (Mesothelioma) or age information, but there is one for 'Sex'. +trait_row = None # No row indicates "Mesothelioma" +age_row = None # No age info found +gender_row = 2 # 'Sex: F' or 'Sex: M' + +# 2) Data Type Conversion Functions +def convert_trait(value: str): + # No trait data is available + return None + +def convert_age(value: str): + # No age data is available + return None + +def convert_gender(value: str): + # Parse "Sex: F" or "Sex: M" -> F -> 0, M -> 1 + val = value.split(':')[-1].strip().lower() + if val == 'f': + return 0 + elif val == 'm': + return 1 + return None + +# 3) Save Metadata (Initial filtering) +is_trait_available = (trait_row is not None) # If we don't have a trait row, then no trait data +is_usable = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4) Clinical Feature Extraction (skip because trait_row is None) +# (No action needed as trait is not available) +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# These identifiers appear to be numeric probe IDs rather than standard human gene symbols. +# Therefore, they require mapping to gene symbols. +print("requires_gene_mapping = True") +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP6: Gene Identifier Mapping + +# 1. Decide which columns store gene IDs and gene symbols. +# From the preview, "ID" in the annotation matches the numeric probe IDs. +# The "gene_assignment" column contains text referencing gene symbols. + +prob_col = "ID" +gene_col = "gene_assignment" + +# 2. Get a gene mapping dataframe from the annotation df. +mapping_df = get_gene_mapping(gene_annotation, prob_col, gene_col) + +# 3. Convert probe-level measurements to gene-level expression data. +gene_data = apply_gene_mapping(gene_data, mapping_df) + +# For verification, print the shape of the resulting dataframe and the first few gene symbols in the index. +print("Gene data shape:", gene_data.shape) +print("First 10 gene symbols in the mapped dataframe index:", list(gene_data.index[:10])) +import os +import pandas as pd + +# STEP 7: Data Normalization and Linking + +# First, check if the clinical CSV file exists. If it does not, we cannot proceed with trait-based linking. +if not os.path.exists(out_clinical_data_file): + # No trait data file => dataset is not usable for trait analysis + df_null = pd.DataFrame() + is_biased = True # Arbitrary boolean to satisfy function requirement + validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=is_biased, + df=df_null, + note="No trait data file found; dataset not usable for trait analysis." + ) + +else: + # 1. Normalize the mapped gene expression data using known gene symbol synonyms, then save. + normalized_gene_data = normalize_gene_symbols_in_index(gene_data) + normalized_gene_data.to_csv(out_gene_data_file) + + # 2. Load the previously extracted clinical CSV. + selected_clinical_df = pd.read_csv(out_clinical_data_file) + # If we had a single-row trait, rename row 0 to the trait name (example usage). + selected_clinical_df = selected_clinical_df.rename(index={0: trait}) + + # Combine these as our final clinical data; in this dataset, we only have trait info (if any). + combined_clinical_df = selected_clinical_df + + # Link the clinical and genetic data by matching sample IDs in columns. + linked_data = geo_link_clinical_genetic_data(combined_clinical_df, normalized_gene_data) + + # 3. Handle missing values in the linked data (drop incomplete rows/columns, then impute). + processed_data = handle_missing_values(linked_data, trait) + + # 4. Check trait bias and remove any biased demographic features (if any). + trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + + # 5. Final validation and metadata saving. + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=processed_data, + note="Completed trait-based preprocessing." + ) + + # 6. If final dataset is usable, save. Otherwise, skip. + if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Mesothelioma/code/GSE163721.py b/p1/preprocess/Mesothelioma/code/GSE163721.py new file mode 100644 index 0000000000000000000000000000000000000000..076a1ecbbb320593f9abff208e587935ce888f81 --- /dev/null +++ b/p1/preprocess/Mesothelioma/code/GSE163721.py @@ -0,0 +1,154 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Mesothelioma" +cohort = "GSE163721" + +# Input paths +in_trait_dir = "../DATA/GEO/Mesothelioma" +in_cohort_dir = "../DATA/GEO/Mesothelioma/GSE163721" + +# Output paths +out_data_file = "./output/preprocess/1/Mesothelioma/GSE163721.csv" +out_gene_data_file = "./output/preprocess/1/Mesothelioma/gene_data/GSE163721.csv" +out_clinical_data_file = "./output/preprocess/1/Mesothelioma/clinical_data/GSE163721.csv" +json_path = "./output/preprocess/1/Mesothelioma/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1. Gene expression data availability +is_gene_available = True # Based on the background info, this is a microarray dataset for gene expression. + +# 2. Variable Availability +# The sample characteristics contain only one key (0) with a single unique value: "tissue type: Tumor". +# This is constant and does not provide useful variation for trait, age, or gender. Thus, they are not available. +trait_row = None +age_row = None +gender_row = None + +# 2.2 Data Type Conversion +# Even though these variables are not available, we define the required converter functions as placeholders. +def convert_trait(x: str): + return None + +def convert_age(x: str): + return None + +def convert_gender(x: str): + return None + +# 3. Save Metadata (initial filtering) +# We treat the dataset as if trait data is unavailable because 'trait_row' is None (no variation). +# This will mark the dataset as not usable at this stage but still record the metadata. +is_trait_available = False +_ = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4. Clinical Feature Extraction +# Since 'trait_row' is None, we skip clinical data extraction. +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# Based on the given index ['1','2','3', ...], these appear to be numeric IDs rather than standard human gene symbols +# Therefore, they likely need to be mapped to actual gene symbols +print("requires_gene_mapping = True") +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP: Gene Identifier Mapping + +# 1. Identify the columns in 'gene_annotation' that correspond to the gene expression IDs and the gene symbols. +# From the preview, 'ID' matches the probe IDs (1,2,3...) in our gene data, and 'Gene Symbol' contains the gene symbols. + +# 2. Get the gene mapping dataframe. +mapping_df = get_gene_mapping(annotation=gene_annotation, prob_col='ID', gene_col='Gene Symbol') + +# 3. Convert probe-level measurements to gene-level expression. +gene_data = apply_gene_mapping(expression_df=gene_data, mapping_df=mapping_df) +import os +import pandas as pd + +# STEP 7: Data Normalization and Linking + +# First, check if the clinical CSV file exists. If it does not, we cannot proceed with trait-based linking. +if not os.path.exists(out_clinical_data_file): + # No trait data file => dataset is not usable for trait analysis + df_null = pd.DataFrame() + is_biased = True # Arbitrary boolean to satisfy function requirement + validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=is_biased, + df=df_null, + note="No trait data file found; dataset not usable for trait analysis." + ) + +else: + # 1. Normalize the mapped gene expression data using known gene symbol synonyms, then save. + normalized_gene_data = normalize_gene_symbols_in_index(gene_data) + normalized_gene_data.to_csv(out_gene_data_file) + + # 2. Load the previously extracted clinical CSV. + selected_clinical_df = pd.read_csv(out_clinical_data_file) + # If we had a single-row trait, rename row 0 to the trait name (example usage). + selected_clinical_df = selected_clinical_df.rename(index={0: trait}) + + # Combine these as our final clinical data; in this dataset, we only have trait info (if any). + combined_clinical_df = selected_clinical_df + + # Link the clinical and genetic data by matching sample IDs in columns. + linked_data = geo_link_clinical_genetic_data(combined_clinical_df, normalized_gene_data) + + # 3. Handle missing values in the linked data (drop incomplete rows/columns, then impute). + processed_data = handle_missing_values(linked_data, trait) + + # 4. Check trait bias and remove any biased demographic features (if any). + trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + + # 5. Final validation and metadata saving. + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=processed_data, + note="Completed trait-based preprocessing." + ) + + # 6. If final dataset is usable, save. Otherwise, skip. + if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Mesothelioma/code/GSE163722.py b/p1/preprocess/Mesothelioma/code/GSE163722.py new file mode 100644 index 0000000000000000000000000000000000000000..3f489b7d0f55466b0803d46aa9d2023096eb3b7f --- /dev/null +++ b/p1/preprocess/Mesothelioma/code/GSE163722.py @@ -0,0 +1,86 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Mesothelioma" +cohort = "GSE163722" + +# Input paths +in_trait_dir = "../DATA/GEO/Mesothelioma" +in_cohort_dir = "../DATA/GEO/Mesothelioma/GSE163722" + +# Output paths +out_data_file = "./output/preprocess/1/Mesothelioma/GSE163722.csv" +out_gene_data_file = "./output/preprocess/1/Mesothelioma/gene_data/GSE163722.csv" +out_clinical_data_file = "./output/preprocess/1/Mesothelioma/clinical_data/GSE163722.csv" +json_path = "./output/preprocess/1/Mesothelioma/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1. Gene Expression Data Availability +# The study is titled "Association of RERG Expression to Female Survival Advantage..." +# This suggests that gene-level expression was measured. Therefore: +is_gene_available = True + +# 2. Variable Availability and Data Type Conversion +# From the sample characteristics dictionary {0: ['tissue type: Tumor']}, +# we see no row providing diverse values for 'Mesothelioma' status, age, or gender. +# Thus, all three are unavailable (constant or missing). + +trait_row = None +age_row = None +gender_row = None + +# Define the conversion functions. Although no data is available, we still write them. + +def convert_trait(value: str): + """ + Convert the trait value to the chosen data type. + Since 'trait' is unavailable in this dataset, we will simply return None. + """ + return None + +def convert_age(value: str): + """ + Convert the age value to the chosen data type (normally continuous). + Since 'age' is unavailable, we return None. + """ + return None + +def convert_gender(value: str): + """ + Convert the gender value to the chosen data type (binary). + Since 'gender' is unavailable, we return None. + """ + return None + +# 3. Save Metadata (Initial filtering) +# If 'trait_row' is None, set is_trait_available=False +is_trait_available = (trait_row is not None) + +is_usable = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4. Clinical Feature Extraction +# Since 'trait_row' is None, skip extracting clinical features. \ No newline at end of file diff --git a/p1/preprocess/Mesothelioma/code/GSE248514.py b/p1/preprocess/Mesothelioma/code/GSE248514.py new file mode 100644 index 0000000000000000000000000000000000000000..a97373779a77de611fe6d0557f6123393b6c3248 --- /dev/null +++ b/p1/preprocess/Mesothelioma/code/GSE248514.py @@ -0,0 +1,167 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Mesothelioma" +cohort = "GSE248514" + +# Input paths +in_trait_dir = "../DATA/GEO/Mesothelioma" +in_cohort_dir = "../DATA/GEO/Mesothelioma/GSE248514" + +# Output paths +out_data_file = "./output/preprocess/1/Mesothelioma/GSE248514.csv" +out_gene_data_file = "./output/preprocess/1/Mesothelioma/gene_data/GSE248514.csv" +out_clinical_data_file = "./output/preprocess/1/Mesothelioma/clinical_data/GSE248514.csv" +json_path = "./output/preprocess/1/Mesothelioma/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1. Gene Expression Data Availability +# Based on the background information that mRNA gene expression profiling was performed, +# we set is_gene_available to True. +is_gene_available = True + +# -------------------------------------------------------------------------------- +# 2. Variable Availability + +# For our trait "Mesothelioma", the dictionary solely shows "tissue: mesothelioma" at row 2, +# which has only one unique value (no variability). Hence, treat it as not available (None). +trait_row = None + +# No row in the dictionary indicates age. Hence, age is not available. +age_row = None + +# Row 3 has two distinct values: "gender: Male" and "gender: Female". So gender is available. +gender_row = 3 + +# -------------------------------------------------------------------------------- +# 2.2 Data Type Conversion + +def convert_trait(x: str): + """ + Convert trait values to a chosen data type. + Since trait is not available, we keep a placeholder that always returns None. + """ + return None + +def convert_age(x: str): + """ + Convert age values to continuous type if available. + Since age is not available, this is a placeholder returning None. + """ + return None + +def convert_gender(x: str): + """ + Convert gender values into binary values: + - Female -> 0 + - Male -> 1 + Any unmapped or unknown value -> None + """ + val = x.split(":", 1)[-1].strip().lower() + if val == "female": + return 0 + elif val == "male": + return 1 + else: + return None + +# -------------------------------------------------------------------------------- +# 3. Save Metadata (Initial Filtering) +is_trait_available = (trait_row is not None) + +is_usable = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# -------------------------------------------------------------------------------- +# 4. Clinical Feature Extraction +# Skip this step because trait_row is None (trait data is not available). +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# After reviewing the provided gene identifiers (A2M, ABCF1, ACVR1C, etc.), +# they appear to be standard human gene symbols that do not require mapping. +print("\nrequires_gene_mapping = False") +import os +import pandas as pd + +# STEP 7: Data Normalization and Linking + +# First, check if the clinical CSV file exists. If it does not, we cannot proceed with trait-based linking. +if not os.path.exists(out_clinical_data_file): + # No trait data file => dataset is not usable for trait analysis + df_null = pd.DataFrame() + is_biased = True # Arbitrary boolean to satisfy function requirement + validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=is_biased, + df=df_null, + note="No trait data file found; dataset not usable for trait analysis." + ) + +else: + # 1. Normalize the mapped gene expression data using known gene symbol synonyms, then save. + normalized_gene_data = normalize_gene_symbols_in_index(gene_data) + normalized_gene_data.to_csv(out_gene_data_file) + + # 2. Load the previously extracted clinical CSV. + selected_clinical_df = pd.read_csv(out_clinical_data_file) + # If we had a single-row trait, rename row 0 to the trait name (example usage). + selected_clinical_df = selected_clinical_df.rename(index={0: trait}) + + # Combine these as our final clinical data; in this dataset, we only have trait info (if any). + combined_clinical_df = selected_clinical_df + + # Link the clinical and genetic data by matching sample IDs in columns. + linked_data = geo_link_clinical_genetic_data(combined_clinical_df, normalized_gene_data) + + # 3. Handle missing values in the linked data (drop incomplete rows/columns, then impute). + processed_data = handle_missing_values(linked_data, trait) + + # 4. Check trait bias and remove any biased demographic features (if any). + trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + + # 5. Final validation and metadata saving. + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=processed_data, + note="Completed trait-based preprocessing." + ) + + # 6. If final dataset is usable, save. Otherwise, skip. + if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Mesothelioma/code/GSE64738.py b/p1/preprocess/Mesothelioma/code/GSE64738.py new file mode 100644 index 0000000000000000000000000000000000000000..d9f03822134b01b259c4f15cbc53b2eddd9046e5 --- /dev/null +++ b/p1/preprocess/Mesothelioma/code/GSE64738.py @@ -0,0 +1,157 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Mesothelioma" +cohort = "GSE64738" + +# Input paths +in_trait_dir = "../DATA/GEO/Mesothelioma" +in_cohort_dir = "../DATA/GEO/Mesothelioma/GSE64738" + +# Output paths +out_data_file = "./output/preprocess/1/Mesothelioma/GSE64738.csv" +out_gene_data_file = "./output/preprocess/1/Mesothelioma/gene_data/GSE64738.csv" +out_clinical_data_file = "./output/preprocess/1/Mesothelioma/clinical_data/GSE64738.csv" +json_path = "./output/preprocess/1/Mesothelioma/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1. Gene Expression Data Availability +is_gene_available = True # Based on microarray description, it likely contains gene expression data. + +# 2. Variable Availability +# From the sample characteristics, all samples are mesothelioma (no variation in disease status). +# There's no mention of age or gender. Therefore, set all rows to None. +trait_row = None +age_row = None +gender_row = None + +# 2.2 Data Type Conversion +# Define the conversion functions (they won't be used here, but we must still define them). +def convert_trait(value: str): + return None + +def convert_age(value: str): + return None + +def convert_gender(value: str): + return None + +# 3. Save Metadata +is_trait_available = (trait_row is not None) +is_usable = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4. Clinical Feature Extraction +# Since trait_row is None, we skip the clinical extraction step. +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# Based on the given identifiers (e.g., "1007_s_at", "1053_at", etc.), +# these appear to be Affymetrix probe set IDs, not standard human gene symbols. +# Therefore, we conclude that gene mapping to official gene symbols is required. + +print("requires_gene_mapping = True") +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP: Gene Identifier Mapping + +# 1. Decide which columns to use for probe IDs and gene symbols +# From the annotation preview, "ID" matches the probe identifiers +# and "Gene Symbol" contains the gene symbols. +probe_col = "ID" +gene_symbol_col = "Gene Symbol" + +# 2. Get a gene mapping dataframe +mapping_df = get_gene_mapping(gene_annotation, prob_col=probe_col, gene_col=gene_symbol_col) + +# 3. Convert probe-level measurements to gene expression data +gene_data = apply_gene_mapping(gene_data, mapping_df) +import os +import pandas as pd + +# STEP 7: Data Normalization and Linking + +# First, check if the clinical CSV file exists. If it does not, we cannot proceed with trait-based linking. +if not os.path.exists(out_clinical_data_file): + # No trait data file => dataset is not usable for trait analysis + df_null = pd.DataFrame() + is_biased = True # Arbitrary boolean to satisfy function requirement + validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=is_biased, + df=df_null, + note="No trait data file found; dataset not usable for trait analysis." + ) + +else: + # 1. Normalize the mapped gene expression data using known gene symbol synonyms, then save. + normalized_gene_data = normalize_gene_symbols_in_index(gene_data) + normalized_gene_data.to_csv(out_gene_data_file) + + # 2. Load the previously extracted clinical CSV. + selected_clinical_df = pd.read_csv(out_clinical_data_file) + # If we had a single-row trait, rename row 0 to the trait name (example usage). + selected_clinical_df = selected_clinical_df.rename(index={0: trait}) + + # Combine these as our final clinical data; in this dataset, we only have trait info (if any). + combined_clinical_df = selected_clinical_df + + # Link the clinical and genetic data by matching sample IDs in columns. + linked_data = geo_link_clinical_genetic_data(combined_clinical_df, normalized_gene_data) + + # 3. Handle missing values in the linked data (drop incomplete rows/columns, then impute). + processed_data = handle_missing_values(linked_data, trait) + + # 4. Check trait bias and remove any biased demographic features (if any). + trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + + # 5. Final validation and metadata saving. + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=processed_data, + note="Completed trait-based preprocessing." + ) + + # 6. If final dataset is usable, save. Otherwise, skip. + if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Mesothelioma/code/GSE68950.py b/p1/preprocess/Mesothelioma/code/GSE68950.py new file mode 100644 index 0000000000000000000000000000000000000000..627413300e3ad688daeeb113dd8e01154f9d7c20 --- /dev/null +++ b/p1/preprocess/Mesothelioma/code/GSE68950.py @@ -0,0 +1,151 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Mesothelioma" +cohort = "GSE68950" + +# Input paths +in_trait_dir = "../DATA/GEO/Mesothelioma" +in_cohort_dir = "../DATA/GEO/Mesothelioma/GSE68950" + +# Output paths +out_data_file = "./output/preprocess/1/Mesothelioma/GSE68950.csv" +out_gene_data_file = "./output/preprocess/1/Mesothelioma/gene_data/GSE68950.csv" +out_clinical_data_file = "./output/preprocess/1/Mesothelioma/clinical_data/GSE68950.csv" +json_path = "./output/preprocess/1/Mesothelioma/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1. Gene Expression Data Availability +is_gene_available = True # "Assay Type: Gene Expression" is indicated in the background + +# 2. Variable Availability and Data Type Conversion + +# 2.1 Data Availability +# After checking row #1's values, we see none of the samples actually mention "mesothelioma". +# So the trait would be constant (all 0). Thus we treat it as unavailable. +trait_row = None +age_row = None +gender_row = None + +# 2.2 Data Type Conversion +def convert_trait(x: str) -> Optional[int]: + # No usable trait data. Always return None. + return None + +def convert_age(x: str) -> Optional[float]: + # No age data, return None. + return None + +def convert_gender(x: str) -> Optional[int]: + # No gender data, return None. + return None + +# 3. Save Metadata (Initial Filtering) +is_trait_available = (trait_row is not None) +is_usable = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4. Clinical Feature Extraction +# Since trait_row is None, we skip this step. +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +print("requires_gene_mapping = True") +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP6: Gene Identifier Mapping + +# 1 & 2. Determine the correct columns in gene_annotation and extract the mapping dataframe. +mapping_df = get_gene_mapping(gene_annotation, prob_col='ID', gene_col='Gene Symbol') + +# 3. Convert probe-level measurements to gene-level expression data. +gene_data = apply_gene_mapping(gene_data, mapping_df) +import os +import pandas as pd + +# STEP 7: Data Normalization and Linking + +# First, check if the clinical CSV file exists. If it does not, we cannot proceed with trait-based linking. +if not os.path.exists(out_clinical_data_file): + # No trait data file => dataset is not usable for trait analysis + df_null = pd.DataFrame() + is_biased = True # Arbitrary boolean to satisfy function requirement + validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=is_biased, + df=df_null, + note="No trait data file found; dataset not usable for trait analysis." + ) + +else: + # 1. Normalize the mapped gene expression data using known gene symbol synonyms, then save. + normalized_gene_data = normalize_gene_symbols_in_index(gene_data) + normalized_gene_data.to_csv(out_gene_data_file) + + # 2. Load the previously extracted clinical CSV. + selected_clinical_df = pd.read_csv(out_clinical_data_file) + # If we had a single-row trait, rename row 0 to the trait name (example usage). + selected_clinical_df = selected_clinical_df.rename(index={0: trait}) + + # Combine these as our final clinical data; in this dataset, we only have trait info (if any). + combined_clinical_df = selected_clinical_df + + # Link the clinical and genetic data by matching sample IDs in columns. + linked_data = geo_link_clinical_genetic_data(combined_clinical_df, normalized_gene_data) + + # 3. Handle missing values in the linked data (drop incomplete rows/columns, then impute). + processed_data = handle_missing_values(linked_data, trait) + + # 4. Check trait bias and remove any biased demographic features (if any). + trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + + # 5. Final validation and metadata saving. + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=processed_data, + note="Completed trait-based preprocessing." + ) + + # 6. If final dataset is usable, save. Otherwise, skip. + if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Mesothelioma/code/TCGA.py b/p1/preprocess/Mesothelioma/code/TCGA.py new file mode 100644 index 0000000000000000000000000000000000000000..ee6257444ed01c4ff2e05095ab7e500e544675b0 --- /dev/null +++ b/p1/preprocess/Mesothelioma/code/TCGA.py @@ -0,0 +1,132 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Mesothelioma" + +# Input paths +tcga_root_dir = "../DATA/TCGA" + +# Output paths +out_data_file = "./output/preprocess/1/Mesothelioma/TCGA.csv" +out_gene_data_file = "./output/preprocess/1/Mesothelioma/gene_data/TCGA.csv" +out_clinical_data_file = "./output/preprocess/1/Mesothelioma/clinical_data/TCGA.csv" +json_path = "./output/preprocess/1/Mesothelioma/cohort_info.json" + +import os +import pandas as pd + +# List of subdirectories provided in the instructions: +subdirectories = [ + 'CrawlData.ipynb', '.DS_Store', 'TCGA_lower_grade_glioma_and_glioblastoma_(GBMLGG)', + 'TCGA_Uterine_Carcinosarcoma_(UCS)', 'TCGA_Thyroid_Cancer_(THCA)', 'TCGA_Thymoma_(THYM)', + 'TCGA_Testicular_Cancer_(TGCT)', 'TCGA_Stomach_Cancer_(STAD)', 'TCGA_Sarcoma_(SARC)', + 'TCGA_Rectal_Cancer_(READ)', 'TCGA_Prostate_Cancer_(PRAD)', 'TCGA_Pheochromocytoma_Paraganglioma_(PCPG)', + 'TCGA_Pancreatic_Cancer_(PAAD)', 'TCGA_Ovarian_Cancer_(OV)', 'TCGA_Ocular_melanomas_(UVM)', + 'TCGA_Mesothelioma_(MESO)', 'TCGA_Melanoma_(SKCM)', 'TCGA_Lung_Squamous_Cell_Carcinoma_(LUSC)', + 'TCGA_Lung_Cancer_(LUNG)', 'TCGA_Lung_Adenocarcinoma_(LUAD)', 'TCGA_Lower_Grade_Glioma_(LGG)', + 'TCGA_Liver_Cancer_(LIHC)', 'TCGA_Large_Bcell_Lymphoma_(DLBC)', 'TCGA_Kidney_Papillary_Cell_Carcinoma_(KIRP)', + 'TCGA_Kidney_Clear_Cell_Carcinoma_(KIRC)', 'TCGA_Kidney_Chromophobe_(KICH)', 'TCGA_Head_and_Neck_Cancer_(HNSC)', + 'TCGA_Glioblastoma_(GBM)', 'TCGA_Esophageal_Cancer_(ESCA)', 'TCGA_Endometrioid_Cancer_(UCEC)', + 'TCGA_Colon_and_Rectal_Cancer_(COADREAD)', 'TCGA_Colon_Cancer_(COAD)', 'TCGA_Cervical_Cancer_(CESC)', + 'TCGA_Breast_Cancer_(BRCA)', 'TCGA_Bladder_Cancer_(BLCA)', 'TCGA_Bile_Duct_Cancer_(CHOL)', + 'TCGA_Adrenocortical_Cancer_(ACC)', 'TCGA_Acute_Myeloid_Leukemia_(LAML)' +] + +# Synonyms or keywords related to Mesothelioma +meso_synonyms = ["mesothelioma", "meso"] + +selected_subdirectory = None +for subdir in subdirectories: + subdir_lower = subdir.lower() + if any(syn in subdir_lower for syn in meso_synonyms): + selected_subdirectory = subdir + break + +if not selected_subdirectory: + # If no matching directory is found, mark dataset as unavailable + is_final = False + is_gene_available = False + is_trait_available = False + _ = validate_and_save_cohort_info( + is_final=is_final, + cohort="TCGA", + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available + ) + print(f"No suitable directory found for '{trait}'. Skipped this trait.") +else: + # Step 2: Identify clinicalMatrix file and PANCAN file + cohort_dir = os.path.join(tcga_root_dir, selected_subdirectory) + clinical_file_path, genetic_file_path = tcga_get_relevant_filepaths(cohort_dir) + + # Step 3: Load both files as dataframes + clinical_df = pd.read_csv(clinical_file_path, index_col=0, sep='\t') + genetic_df = pd.read_csv(genetic_file_path, index_col=0, sep='\t') + + # Step 4: Print the column names of the clinical data + print("Clinical data columns:") + print(list(clinical_df.columns)) +# Step 1: Identify candidate columns +candidate_age_cols = ["age_at_initial_pathologic_diagnosis", "asbestos_exposure_age", "asbestos_exposure_age_last", "days_to_birth"] +candidate_gender_cols = ["gender"] + +# Print them in the required format +print(f"candidate_age_cols = {candidate_age_cols}") +print(f"candidate_gender_cols = {candidate_gender_cols}") + +# Step 2: Extract and preview data +if candidate_age_cols: + age_df = clinical_df[candidate_age_cols] + print("Age columns preview:", preview_df(age_df, n=5)) + +if candidate_gender_cols: + gender_df = clinical_df[candidate_gender_cols] + print("Gender columns preview:", preview_df(gender_df, n=5)) +age_col = "age_at_initial_pathologic_diagnosis" +gender_col = "gender" + +print("Chosen age_col:", age_col) +print("Chosen gender_col:", gender_col) +# 1. Extract and standardize the clinical features +selected_clinical_df = tcga_select_clinical_features( + clinical_df=clinical_df, + trait=trait, + age_col=age_col, + gender_col=gender_col +) + +# 2. Normalize gene symbols in the expression data and save +gene_df = normalize_gene_symbols_in_index(genetic_df) +gene_df.to_csv(out_gene_data_file) + +# 3. Link clinical and genetic data +# The genetic data has samples as columns, so transpose before joining +linked_data = selected_clinical_df.join(gene_df.T, how='inner') + +# 4. Handle missing values +processed_data = handle_missing_values(linked_data, trait_col=trait) + +# 5. Determine and remove biased features +biased_trait, processed_data = judge_and_remove_biased_features(processed_data, trait) + +# 6. Final quality validation +gene_cols = [col for col in processed_data.columns if col not in [trait, "Age", "Gender"]] +is_gene_available = len(gene_cols) > 0 +is_trait_available = trait in processed_data.columns + +is_usable = validate_and_save_cohort_info( + is_final=True, + cohort="TCGA", + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available, + is_biased=biased_trait, + df=processed_data, + note="Final data processing for Kidney_Chromophobe" +) + +# 7. Save linked data if usable +if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Mesothelioma/cohort_info.json b/p1/preprocess/Mesothelioma/cohort_info.json new file mode 100644 index 0000000000000000000000000000000000000000..7cccf9790f0bef695cba542fc7b962b793247071 --- /dev/null +++ b/p1/preprocess/Mesothelioma/cohort_info.json @@ -0,0 +1 @@ +{"GSE68950": {"is_usable": true, "is_gene_available": true, "is_trait_available": true, "is_available": true, "is_biased": false, "has_age": false, "has_gender": false, "sample_size": 798, "note": "Completed trait-based preprocessing."}, "GSE64738": {"is_usable": false, "is_gene_available": false, "is_trait_available": false, "is_available": false, "is_biased": null, "has_age": null, "has_gender": null, "sample_size": null, "note": "No trait data file found; dataset not usable for trait analysis."}, "GSE248514": {"is_usable": false, "is_gene_available": false, "is_trait_available": false, "is_available": false, "is_biased": null, "has_age": null, "has_gender": null, "sample_size": null, "note": "No trait data file found; dataset not usable for trait analysis."}, "GSE163722": {"is_usable": false, "is_gene_available": true, "is_trait_available": false, "is_available": false, "is_biased": null, "has_age": null, "has_gender": null, "sample_size": null, "note": null}, "GSE163721": {"is_usable": false, "is_gene_available": false, "is_trait_available": false, "is_available": false, "is_biased": null, "has_age": null, "has_gender": null, "sample_size": null, "note": "No trait data file found; dataset not usable for trait analysis."}, "GSE163720": {"is_usable": false, "is_gene_available": false, "is_trait_available": false, "is_available": false, "is_biased": null, "has_age": null, "has_gender": null, "sample_size": null, "note": "No trait data file found; dataset not usable for trait analysis."}, "GSE131027": {"is_usable": true, "is_gene_available": true, "is_trait_available": true, "is_available": true, "is_biased": false, "has_age": false, "has_gender": false, "sample_size": 92, "note": "Completed trait-based preprocessing."}, "GSE117668": {"is_usable": true, "is_gene_available": true, "is_trait_available": true, "is_available": true, "is_biased": false, "has_age": false, "has_gender": false, "sample_size": 48, "note": "Completed trait-based preprocessing."}, "GSE112154": {"is_usable": false, "is_gene_available": true, "is_trait_available": true, "is_available": true, "is_biased": true, "has_age": false, "has_gender": false, "sample_size": 50, "note": "Completed trait-based preprocessing."}, "GSE107754": {"is_usable": false, "is_gene_available": true, "is_trait_available": true, "is_available": true, "is_biased": true, "has_age": false, "has_gender": false, "sample_size": 84, "note": "Completed trait-based preprocessing."}, "TCGA": {"is_usable": false, "is_gene_available": true, "is_trait_available": true, "is_available": true, "is_biased": true, 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a/p1/preprocess/Metabolic_Rate/code/GSE101492.py b/p1/preprocess/Metabolic_Rate/code/GSE101492.py new file mode 100644 index 0000000000000000000000000000000000000000..019f393e1472621ccb0a817c8be6c9c3586039dd --- /dev/null +++ b/p1/preprocess/Metabolic_Rate/code/GSE101492.py @@ -0,0 +1,188 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Metabolic_Rate" +cohort = "GSE101492" + +# Input paths +in_trait_dir = "../DATA/GEO/Metabolic_Rate" +in_cohort_dir = "../DATA/GEO/Metabolic_Rate/GSE101492" + +# Output paths +out_data_file = "./output/preprocess/1/Metabolic_Rate/GSE101492.csv" +out_gene_data_file = "./output/preprocess/1/Metabolic_Rate/gene_data/GSE101492.csv" +out_clinical_data_file = "./output/preprocess/1/Metabolic_Rate/clinical_data/GSE101492.csv" +json_path = "./output/preprocess/1/Metabolic_Rate/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1) Determine if the dataset likely contains gene expression data. +is_gene_available = True # Based on the presence of lncRNA expression data (not purely miRNA or methylation). + +# 2) Assess the availability of variables (trait, age, gender) and define conversion functions. + +# Based on the reviewers' suggestion and the sample characteristics, +# we infer "insulin sensitivity" can serve as our Metabolic_Rate measure. +trait_row = 3 + +# The age data is in key=2, containing multiple distinct values. +age_row = 2 + +# The gender data is at key=1, but it's always "female" (only one unique value), so it's excluded. +gender_row = None + +# 2.2 Define the conversion functions. +def convert_trait(value: str): + # Expecting a format like "insulin sensitivity: insulin sensitive" + parts = value.split(':') + if len(parts) > 1: + val_str = parts[-1].strip().lower() + if 'sensitive' in val_str: + return 0 + elif 'resistant' in val_str: + return 1 + return None + +def convert_age(value: str): + # Expecting a format like "age: 39" + parts = value.split(':') + if len(parts) > 1: + num_str = parts[-1].strip() + try: + return int(num_str) + except ValueError: + return None + return None + +def convert_gender(value: str): + # Not used since gender_row is None, but define it for completeness. + return None + +# 3) Save metadata via initial filtering. +is_trait_available = (trait_row is not None) +is_usable = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4) If trait_row is not None, extract clinical features. +if trait_row is not None: + selected_clinical_data = geo_select_clinical_features( + clinical_data, + trait=trait, + trait_row=trait_row, + convert_trait=convert_trait, + age_row=age_row, + convert_age=convert_age, + gender_row=gender_row, + convert_gender=convert_gender + ) + preview_dict = preview_df(selected_clinical_data) + print("Preview of clinical data:", preview_dict) + selected_clinical_data.to_csv(out_clinical_data_file, index=False) +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# Based on observation, the IDs are numeric and do not resemble standard human gene symbols. +# They likely need to be mapped to gene symbols. + +print("requires_gene_mapping = True") +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP: Gene Identifier Mapping + +# 1) Decide which columns correspond to the same probe IDs as the gene expression data and which likely hold gene symbols. +# From the annotation preview, the 'ID' column in gene_annotation matches the probe IDs in gene_data, +# and 'gene_assignment' seems to store the gene-related text (though it may look incomplete). + +# 2) Obtain gene mapping (probe ID to gene symbol or related identifier). +mapping_df = get_gene_mapping(gene_annotation, prob_col='ID', gene_col='gene_assignment') + +# 3) Convert probe-level measurements to gene-level measurements. +gene_data = apply_gene_mapping(gene_data, mapping_df) +import os +import pandas as pd + +# STEP 7: Data Normalization and Linking + +# First, check if the clinical CSV file exists. If it does not, we cannot proceed with trait-based linking. +if not os.path.exists(out_clinical_data_file): + # No trait data file => dataset is not usable for trait analysis + df_null = pd.DataFrame() + is_biased = True # Arbitrary boolean to satisfy function requirement + validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=is_biased, + df=df_null, + note="No trait data file found; dataset not usable for trait analysis." + ) + +else: + # 1. Normalize the mapped gene expression data using known gene symbol synonyms, then save. + normalized_gene_data = normalize_gene_symbols_in_index(gene_data) + normalized_gene_data.to_csv(out_gene_data_file) + + # 2. Load the previously extracted clinical CSV. + selected_clinical_df = pd.read_csv(out_clinical_data_file) + # If we had a single-row trait, rename row 0 to the trait name (example usage). + selected_clinical_df = selected_clinical_df.rename(index={0: trait}) + + # Combine these as our final clinical data; in this dataset, we only have trait info (if any). + combined_clinical_df = selected_clinical_df + + # Link the clinical and genetic data by matching sample IDs in columns. + linked_data = geo_link_clinical_genetic_data(combined_clinical_df, normalized_gene_data) + + # 3. Handle missing values in the linked data (drop incomplete rows/columns, then impute). + processed_data = handle_missing_values(linked_data, trait) + + # 4. Check trait bias and remove any biased demographic features (if any). + trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + + # 5. Final validation and metadata saving. + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=processed_data, + note="Completed trait-based preprocessing." + ) + + # 6. If final dataset is usable, save. Otherwise, skip. + if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Metabolic_Rate/code/GSE106800.py b/p1/preprocess/Metabolic_Rate/code/GSE106800.py new file mode 100644 index 0000000000000000000000000000000000000000..110aeb20ca2947c5426aa6c9ed71568151cc005b --- /dev/null +++ b/p1/preprocess/Metabolic_Rate/code/GSE106800.py @@ -0,0 +1,196 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Metabolic_Rate" +cohort = "GSE106800" + +# Input paths +in_trait_dir = "../DATA/GEO/Metabolic_Rate" +in_cohort_dir = "../DATA/GEO/Metabolic_Rate/GSE106800" + +# Output paths +out_data_file = "./output/preprocess/1/Metabolic_Rate/GSE106800.csv" +out_gene_data_file = "./output/preprocess/1/Metabolic_Rate/gene_data/GSE106800.csv" +out_clinical_data_file = "./output/preprocess/1/Metabolic_Rate/clinical_data/GSE106800.csv" +json_path = "./output/preprocess/1/Metabolic_Rate/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1. Determine if the dataset contains gene expression data +# Based on the series summary and mention of microarray analysis, we conclude it is likely gene expression data. +is_gene_available = True + +# 2. Variable Availability and Data Type Conversion + +# 2.1 Identify the keys and availability for each variable + +# For the trait "Metabolic_Rate", there is no direct or inferred key in the dictionary with multiple distinct values, +# so we consider it unavailable. +trait_row = None + +# For age data, we see "age (yrs): ..." in key 2 with multiple distinct values. Hence it's available. +age_row = 2 + +# For gender, we have key 0 stating "gender: male" only. It is a constant and thus not useful for association analysis. +gender_row = None + +# 2.2 Define conversion functions + +# convert_trait (not used in practice because trait_row is None, but required by the library function signature) +def convert_trait(value: str) -> Optional[float]: + """ + Convert the 'Metabolic_Rate' trait value to a continuous or binary representation. + Not applicable here, returns None. + """ + return None + +# convert_age +def convert_age(value: str) -> Optional[float]: + """ + Extract the numeric part of 'age (yrs): XX' and convert to a float. + Unknown or invalid values are converted to None. + """ + parts = value.split(':') + if len(parts) < 2: + return None + try: + return float(parts[1].strip()) + except ValueError: + return None + +# convert_gender (not used here because gender_row is None, but defined for completeness) +def convert_gender(value: str) -> Optional[int]: + """ + Convert 'gender: male' to 1 and 'gender: female' to 0, returns None if unknown. + """ + parts = value.split(':') + if len(parts) < 2: + return None + val = parts[1].strip().lower() + if val == 'male': + return 1 + elif val == 'female': + return 0 + return None + +# 3. Save metadata for initial filtering +# Trait data is not available if trait_row is None. +is_trait_available = (trait_row is not None) + +validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4. Clinical feature extraction +# Since trait_row is None (no trait data), we do NOT perform clinical feature extraction. +# No further steps for clinical data extraction are needed here. +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# Based on the numeric IDs observed, these are not typical human gene symbols and likely require mapping. +print("requires_gene_mapping = True") +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP: Gene Identifier Mapping + +# 1. Decide which columns in the annotation data correspond to the probe identifiers +# (matching the gene expression DataFrame index) and which contain gene symbols. +# From the preview, 'ID' matches probe IDs, and 'gene_assignment' describes gene symbols. + +# 2. Build the gene mapping dataframe +mapping_df = get_gene_mapping(gene_annotation, prob_col='ID', gene_col='gene_assignment') + +# 3. Convert probe-level measurements to gene-level expression +gene_data = apply_gene_mapping(gene_data, mapping_df) + +# (Optional) Print shape and a small preview to verify +print("Mapped gene_data shape:", gene_data.shape) +print("Mapped gene_data preview:") +print(gene_data.head()) +import os +import pandas as pd + +# STEP 7: Data Normalization and Linking + +# First, check if the clinical CSV file exists. If it does not, we cannot proceed with trait-based linking. +if not os.path.exists(out_clinical_data_file): + # No trait data file => dataset is not usable for trait analysis + df_null = pd.DataFrame() + is_biased = True # Arbitrary boolean to satisfy function requirement + validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=is_biased, + df=df_null, + note="No trait data file found; dataset not usable for trait analysis." + ) + +else: + # 1. Normalize the mapped gene expression data using known gene symbol synonyms, then save. + normalized_gene_data = normalize_gene_symbols_in_index(gene_data) + normalized_gene_data.to_csv(out_gene_data_file) + + # 2. Load the previously extracted clinical CSV. + selected_clinical_df = pd.read_csv(out_clinical_data_file) + # If we had a single-row trait, rename row 0 to the trait name (example usage). + selected_clinical_df = selected_clinical_df.rename(index={0: trait}) + + # Combine these as our final clinical data; in this dataset, we only have trait info (if any). + combined_clinical_df = selected_clinical_df + + # Link the clinical and genetic data by matching sample IDs in columns. + linked_data = geo_link_clinical_genetic_data(combined_clinical_df, normalized_gene_data) + + # 3. Handle missing values in the linked data (drop incomplete rows/columns, then impute). + processed_data = handle_missing_values(linked_data, trait) + + # 4. Check trait bias and remove any biased demographic features (if any). + trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + + # 5. Final validation and metadata saving. + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=processed_data, + note="Completed trait-based preprocessing." + ) + + # 6. If final dataset is usable, save. Otherwise, skip. + if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Metabolic_Rate/code/GSE151683.py b/p1/preprocess/Metabolic_Rate/code/GSE151683.py new file mode 100644 index 0000000000000000000000000000000000000000..ef6237c97b3762f6201ac777fd7a98c6e3afdaec --- /dev/null +++ b/p1/preprocess/Metabolic_Rate/code/GSE151683.py @@ -0,0 +1,162 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Metabolic_Rate" +cohort = "GSE151683" + +# Input paths +in_trait_dir = "../DATA/GEO/Metabolic_Rate" +in_cohort_dir = "../DATA/GEO/Metabolic_Rate/GSE151683" + +# Output paths +out_data_file = "./output/preprocess/1/Metabolic_Rate/GSE151683.csv" +out_gene_data_file = "./output/preprocess/1/Metabolic_Rate/gene_data/GSE151683.csv" +out_clinical_data_file = "./output/preprocess/1/Metabolic_Rate/clinical_data/GSE151683.csv" +json_path = "./output/preprocess/1/Metabolic_Rate/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# Step 1: Determine if gene expression data is available +is_gene_available = True + +# Step 2: Assess data availability for trait, age, and gender +trait_row = None # No row associated with "Metabolic_Rate" +age_row = 2 # Row 2 contains "age (years): ..." +gender_row = None # All samples appear to be male; no variation + +# Step 2.2: Define conversion functions +def convert_trait(value: str): + # Trait data is not available; return None + return None + +def convert_age(value: str): + # Example format: 'age (years): 42' + # Extract the numeric portion after the colon + val = value.split(':')[-1].strip() + try: + return float(val) + except ValueError: + return None + +def convert_gender(value: str): + # Not used here because gender data is not available + # but provided for completeness + val = value.split(':')[-1].strip().lower() + if val == 'male': + return 1 + elif val == 'female': + return 0 + return None + +# Step 3: Perform initial filtering and save metadata +is_usable = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=(trait_row is not None) +) + +# Step 4: Since trait_row is None, we skip clinical feature extraction. +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# These identifiers (e.g., '1007_s_at', '1053_at') appear to be Affymetrix probe IDs rather than human gene symbols. +# Therefore, gene mapping to standard gene symbols is required. +print("requires_gene_mapping = True") +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP: Gene Identifier Mapping + +# 1. Determine the appropriate columns for probe IDs and gene symbols +probe_col = "ID" +symbol_col = "Gene Symbol" + +# 2. Get the gene mapping dataframe +gene_mapping_df = get_gene_mapping(gene_annotation, prob_col=probe_col, gene_col=symbol_col) + +# 3. Apply the gene mapping to convert from probe-level to gene-level expression data +gene_data = apply_gene_mapping(gene_data, gene_mapping_df) +import os +import pandas as pd + +# STEP 7: Data Normalization and Linking + +# First, check if the clinical CSV file exists. If it does not, we cannot proceed with trait-based linking. +if not os.path.exists(out_clinical_data_file): + # No trait data file => dataset is not usable for trait analysis + df_null = pd.DataFrame() + is_biased = True # Arbitrary boolean to satisfy function requirement + validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=is_biased, + df=df_null, + note="No trait data file found; dataset not usable for trait analysis." + ) + +else: + # 1. Normalize the mapped gene expression data using known gene symbol synonyms, then save. + normalized_gene_data = normalize_gene_symbols_in_index(gene_data) + normalized_gene_data.to_csv(out_gene_data_file) + + # 2. Load the previously extracted clinical CSV. + selected_clinical_df = pd.read_csv(out_clinical_data_file) + # If we had a single-row trait, rename row 0 to the trait name (example usage). + selected_clinical_df = selected_clinical_df.rename(index={0: trait}) + + # Combine these as our final clinical data; in this dataset, we only have trait info (if any). + combined_clinical_df = selected_clinical_df + + # Link the clinical and genetic data by matching sample IDs in columns. + linked_data = geo_link_clinical_genetic_data(combined_clinical_df, normalized_gene_data) + + # 3. Handle missing values in the linked data (drop incomplete rows/columns, then impute). + processed_data = handle_missing_values(linked_data, trait) + + # 4. Check trait bias and remove any biased demographic features (if any). + trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + + # 5. Final validation and metadata saving. + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=processed_data, + note="Completed trait-based preprocessing." + ) + + # 6. If final dataset is usable, save. Otherwise, skip. + if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Metabolic_Rate/code/GSE23025.py b/p1/preprocess/Metabolic_Rate/code/GSE23025.py new file mode 100644 index 0000000000000000000000000000000000000000..92fa6f2d79779c60d8fc10e9c361583449a7d74f --- /dev/null +++ b/p1/preprocess/Metabolic_Rate/code/GSE23025.py @@ -0,0 +1,156 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Metabolic_Rate" +cohort = "GSE23025" + +# Input paths +in_trait_dir = "../DATA/GEO/Metabolic_Rate" +in_cohort_dir = "../DATA/GEO/Metabolic_Rate/GSE23025" + +# Output paths +out_data_file = "./output/preprocess/1/Metabolic_Rate/GSE23025.csv" +out_gene_data_file = "./output/preprocess/1/Metabolic_Rate/gene_data/GSE23025.csv" +out_clinical_data_file = "./output/preprocess/1/Metabolic_Rate/clinical_data/GSE23025.csv" +json_path = "./output/preprocess/1/Metabolic_Rate/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1. Determine if the dataset likely contains gene expression data +is_gene_available = True # Based on the background, it's Affymetrix microarray gene expression + +# 2. Variable Availability +# No rows in the sample characteristics dictionary correspond to Metabolic_Rate, age, or gender. +trait_row = None +age_row = None +gender_row = None + +# 2.2 Data Type Conversion Functions +def convert_trait(value: str): + # No trait data available in this dataset; always return None + return None + +def convert_age(value: str): + # No age data available; always return None + return None + +def convert_gender(value: str): + # No gender data available; always return None + return None + +# 3. Save initial filtering metadata +is_trait_available = (trait_row is not None) +validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4. Clinical Feature Extraction +# Since trait_row is None, we skip clinical data extraction. +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# Based on the provided identifiers (e.g., "1007_s_at", "1053_at"), these look like Affymetrix probe IDs, +# not direct human gene symbols. Therefore, gene mapping is required. + +requires_gene_mapping = True +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP: Gene Identifier Mapping + +# 1. Identify columns that store the probe identifier and the gene symbol in 'gene_annotation'. +# From the preview, the "ID" column matches the probe identifiers in `gene_data`, +# and the "Gene Symbol" column stores the actual gene symbols. +mapping_df = get_gene_mapping(gene_annotation, prob_col="ID", gene_col="Gene Symbol") + +# 2. Convert probe-level measurements to gene-level measurements using the mapping dataframe. +gene_data = apply_gene_mapping(gene_data, mapping_df) + +# (Optional) Print the shape of the resulting gene_data for confirmation. +print("Gene expression data shape after mapping:", gene_data.shape) +import os +import pandas as pd + +# STEP 7: Data Normalization and Linking + +# First, check if the clinical CSV file exists. If it does not, we cannot proceed with trait-based linking. +if not os.path.exists(out_clinical_data_file): + # No trait data file => dataset is not usable for trait analysis + df_null = pd.DataFrame() + is_biased = True # Arbitrary boolean to satisfy function requirement + validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=is_biased, + df=df_null, + note="No trait data file found; dataset not usable for trait analysis." + ) + +else: + # 1. Normalize the mapped gene expression data using known gene symbol synonyms, then save. + normalized_gene_data = normalize_gene_symbols_in_index(gene_data) + normalized_gene_data.to_csv(out_gene_data_file) + + # 2. Load the previously extracted clinical CSV. + selected_clinical_df = pd.read_csv(out_clinical_data_file) + # If we had a single-row trait, rename row 0 to the trait name (example usage). + selected_clinical_df = selected_clinical_df.rename(index={0: trait}) + + # Combine these as our final clinical data; in this dataset, we only have trait info (if any). + combined_clinical_df = selected_clinical_df + + # Link the clinical and genetic data by matching sample IDs in columns. + linked_data = geo_link_clinical_genetic_data(combined_clinical_df, normalized_gene_data) + + # 3. Handle missing values in the linked data (drop incomplete rows/columns, then impute). + processed_data = handle_missing_values(linked_data, trait) + + # 4. Check trait bias and remove any biased demographic features (if any). + trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + + # 5. Final validation and metadata saving. + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=processed_data, + note="Completed trait-based preprocessing." + ) + + # 6. If final dataset is usable, save. Otherwise, skip. + if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Metabolic_Rate/code/GSE26440.py b/p1/preprocess/Metabolic_Rate/code/GSE26440.py new file mode 100644 index 0000000000000000000000000000000000000000..07487f736ee439b7e276f90d83ef084e90cf773c --- /dev/null +++ b/p1/preprocess/Metabolic_Rate/code/GSE26440.py @@ -0,0 +1,189 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Metabolic_Rate" +cohort = "GSE26440" + +# Input paths +in_trait_dir = "../DATA/GEO/Metabolic_Rate" +in_cohort_dir = "../DATA/GEO/Metabolic_Rate/GSE26440" + +# Output paths +out_data_file = "./output/preprocess/1/Metabolic_Rate/GSE26440.csv" +out_gene_data_file = "./output/preprocess/1/Metabolic_Rate/gene_data/GSE26440.csv" +out_clinical_data_file = "./output/preprocess/1/Metabolic_Rate/clinical_data/GSE26440.csv" +json_path = "./output/preprocess/1/Metabolic_Rate/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1) Gene Expression Data Availability +# Based on the provided background, this dataset includes gene expression profiling (not purely miRNA or methylation). +is_gene_available = True + +# 2) Variable Availability and Data Type Conversion + +# After reviewing the sample characteristics dictionary, we see: +# - The 'trait' ("Metabolic_Rate") is not listed or inferable => trait_row = None +# - 'age' is available at row=2, with multiple distinct values => age_row = 2 +# - 'gender' is not found => gender_row = None + +trait_row = None +age_row = 2 +gender_row = None + +# Data conversion functions: +def convert_trait(value: str): + """ + For demonstration only; this trait is not found in the data (trait_row=None). + We define this function to comply with naming requirements. + """ + # If this were used, parse and convert to numeric/binary as appropriate. + return None + +def convert_age(value: str): + """ + Convert age (e.g. 'age (years): 9.4') to a float. + Unknown or invalid entries return None. + """ + # Split on colon and strip + parts = value.split(':') + if len(parts) < 2: + return None + val_str = parts[1].strip() + try: + return float(val_str) + except ValueError: + return None + +def convert_gender(value: str): + """ + Convert gender string to 0 (female) or 1 (male). Otherwise None. + Not used in this dataset because gender_row=None. + """ + # Split on colon and strip + parts = value.split(':') + if len(parts) < 2: + return None + val_str = parts[1].strip().lower() + if val_str in ['female', 'f']: + return 0 + elif val_str in ['male', 'm']: + return 1 + else: + return None + +# 3) Save Metadata with initial filtering +is_trait_available = (trait_row is not None) +validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4) Since trait_row is None, skip clinical feature extraction. +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# The gene identifiers appear to be Affymetrix probe IDs, not human gene symbols. +# Therefore, gene mapping is required. +print("\nrequires_gene_mapping = True") +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP: Gene Identifier Mapping + +# 1. Identify the relevant columns for mapping: +# From the preview, 'ID' corresponds to the Affymetrix probe ID that matches the gene expression data index, +# and 'Gene Symbol' stores the gene symbols. + +mapping_df = get_gene_mapping(gene_annotation, prob_col='ID', gene_col='Gene Symbol') + +# 2. Convert probe-level expression data to gene-level expression data +gene_data = apply_gene_mapping(gene_data, mapping_df) + +# Print shape to verify the conversion result +print(f"Gene expression dataframe now has {gene_data.shape[0]} genes and {gene_data.shape[1]} samples.") +import os +import pandas as pd + +# STEP 7: Data Normalization and Linking + +# First, check if the clinical CSV file exists. If it does not, we cannot proceed with trait-based linking. +if not os.path.exists(out_clinical_data_file): + # No trait data file => dataset is not usable for trait analysis + df_null = pd.DataFrame() + is_biased = True # Arbitrary boolean to satisfy function requirement + validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=is_biased, + df=df_null, + note="No trait data file found; dataset not usable for trait analysis." + ) + +else: + # 1. Normalize the mapped gene expression data using known gene symbol synonyms, then save. + normalized_gene_data = normalize_gene_symbols_in_index(gene_data) + normalized_gene_data.to_csv(out_gene_data_file) + + # 2. Load the previously extracted clinical CSV. + selected_clinical_df = pd.read_csv(out_clinical_data_file) + # If we had a single-row trait, rename row 0 to the trait name (example usage). + selected_clinical_df = selected_clinical_df.rename(index={0: trait}) + + # Combine these as our final clinical data; in this dataset, we only have trait info (if any). + combined_clinical_df = selected_clinical_df + + # Link the clinical and genetic data by matching sample IDs in columns. + linked_data = geo_link_clinical_genetic_data(combined_clinical_df, normalized_gene_data) + + # 3. Handle missing values in the linked data (drop incomplete rows/columns, then impute). + processed_data = handle_missing_values(linked_data, trait) + + # 4. Check trait bias and remove any biased demographic features (if any). + trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + + # 5. Final validation and metadata saving. + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=processed_data, + note="Completed trait-based preprocessing." + ) + + # 6. If final dataset is usable, save. Otherwise, skip. + if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Metabolic_Rate/code/GSE40589.py b/p1/preprocess/Metabolic_Rate/code/GSE40589.py new file mode 100644 index 0000000000000000000000000000000000000000..79cb282ec606ce75b7718df44d3a247d4a162026 --- /dev/null +++ b/p1/preprocess/Metabolic_Rate/code/GSE40589.py @@ -0,0 +1,168 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Metabolic_Rate" +cohort = "GSE40589" + +# Input paths +in_trait_dir = "../DATA/GEO/Metabolic_Rate" +in_cohort_dir = "../DATA/GEO/Metabolic_Rate/GSE40589" + +# Output paths +out_data_file = "./output/preprocess/1/Metabolic_Rate/GSE40589.csv" +out_gene_data_file = "./output/preprocess/1/Metabolic_Rate/gene_data/GSE40589.csv" +out_clinical_data_file = "./output/preprocess/1/Metabolic_Rate/clinical_data/GSE40589.csv" +json_path = "./output/preprocess/1/Metabolic_Rate/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1. Gene Expression Data Availability +# Based on the series description, it investigates gene expression in subcutaneous adipose tissue, +# so we consider gene expression data to be available. +is_gene_available = True + +# 2. Variable Availability and Data Type Conversion +# From the provided sample characteristics dictionary, we only have the entry: +# 0: ['tissue: subcutaneous adipose tissue'] +# There is no row containing the actual trait, age, or gender information. +# Therefore, we set all row indices to None. +trait_row = None +age_row = None +gender_row = None + +# Since we have no data for these variables, to comply with the requirement to name these functions: +def convert_trait(value: str): + # Data not available; return None + return None + +def convert_age(value: str): + # Data not available; return None + return None + +def convert_gender(value: str): + # Data not available; return None + return None + +# 3. Save Metadata +# The trait data availability depends on whether trait_row is None. +is_trait_available = (trait_row is not None) + +# Conduct initial filtering on the dataset. +# Because trait_row is None, is_trait_available is False. +validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4. Clinical Feature Extraction +# Since `trait_row` is None, clinical data is not considered available. Skip extraction. +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# These identifiers (e.g., A_23_P100001) are microarray probe IDs rather than standard human gene symbols. +# Hence, they require mapping to gene symbols. + +requires_gene_mapping = True +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP: Gene Identifier Mapping + +# 1. Identify the columns in the annotation dataframe corresponding to the probe IDs and gene symbols. +# From the preview, the "ID" column matches the probe IDs in gene_data, +# and the "GENE_SYMBOL" column contains the gene symbols. + +# 2. Create a gene mapping dataframe. +mapping_df = get_gene_mapping(gene_annotation, prob_col='ID', gene_col='GENE_SYMBOL') + +# 3. Convert the probe-level gene expression data to gene-level data using the mapping. +gene_data = apply_gene_mapping(gene_data, mapping_df) + +# Print some basic info to confirm the transformation +print("Gene expression data shape:", gene_data.shape) +print("First 5 gene symbols after mapping:", gene_data.index[:5]) +import os +import pandas as pd + +# STEP 7: Data Normalization and Linking + +# First, check if the clinical CSV file exists. If it does not, we cannot proceed with trait-based linking. +if not os.path.exists(out_clinical_data_file): + # No trait data file => dataset is not usable for trait analysis + df_null = pd.DataFrame() + is_biased = True # Arbitrary boolean to satisfy function requirement + validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=is_biased, + df=df_null, + note="No trait data file found; dataset not usable for trait analysis." + ) + +else: + # 1. Normalize the mapped gene expression data using known gene symbol synonyms, then save. + normalized_gene_data = normalize_gene_symbols_in_index(gene_data) + normalized_gene_data.to_csv(out_gene_data_file) + + # 2. Load the previously extracted clinical CSV. + selected_clinical_df = pd.read_csv(out_clinical_data_file) + # If we had a single-row trait, rename row 0 to the trait name (example usage). + selected_clinical_df = selected_clinical_df.rename(index={0: trait}) + + # Combine these as our final clinical data; in this dataset, we only have trait info (if any). + combined_clinical_df = selected_clinical_df + + # Link the clinical and genetic data by matching sample IDs in columns. + linked_data = geo_link_clinical_genetic_data(combined_clinical_df, normalized_gene_data) + + # 3. Handle missing values in the linked data (drop incomplete rows/columns, then impute). + processed_data = handle_missing_values(linked_data, trait) + + # 4. Check trait bias and remove any biased demographic features (if any). + trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + + # 5. Final validation and metadata saving. + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=processed_data, + note="Completed trait-based preprocessing." + ) + + # 6. If final dataset is usable, save. Otherwise, skip. + if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Metabolic_Rate/code/GSE40873.py b/p1/preprocess/Metabolic_Rate/code/GSE40873.py new file mode 100644 index 0000000000000000000000000000000000000000..9e841025731f9af95cbacf1134e955d47c7c3cf0 --- /dev/null +++ b/p1/preprocess/Metabolic_Rate/code/GSE40873.py @@ -0,0 +1,166 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Metabolic_Rate" +cohort = "GSE40873" + +# Input paths +in_trait_dir = "../DATA/GEO/Metabolic_Rate" +in_cohort_dir = "../DATA/GEO/Metabolic_Rate/GSE40873" + +# Output paths +out_data_file = "./output/preprocess/1/Metabolic_Rate/GSE40873.csv" +out_gene_data_file = "./output/preprocess/1/Metabolic_Rate/gene_data/GSE40873.csv" +out_clinical_data_file = "./output/preprocess/1/Metabolic_Rate/clinical_data/GSE40873.csv" +json_path = "./output/preprocess/1/Metabolic_Rate/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1. Gene Expression Data Availability +# Based on the series information referencing "genome-wide gene expression analysis", +# we infer that the dataset likely contains actual gene expression data. +is_gene_available = True + +# 2. Variable Availability and Data Type Conversion +# From the provided sample characteristics dictionary, we see no key describing "Metabolic_Rate", +# age, or gender. Hence all are unavailable and have only one option: None. + +trait_row = None +age_row = None +gender_row = None + +# Although no data exists for these variables, create the required conversion functions. +# Here, unknown values or absent data simply return None. + +def convert_trait(value: str): + # Typically, we'd parse something after the colon or apply heuristic rules, + # but since data is unavailable, we return None. + return None + +def convert_age(value: str): + return None + +def convert_gender(value: str): + return None + +# 3. Save Metadata Using Initial Filtering +# Trait data availability depends on trait_row. Since trait_row is None, is_trait_available=False. +is_trait_available = (trait_row is not None) + +validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4. Clinical Feature Extraction (Skip if trait_row is None) +# Since trait_row is None, we do not extract clinical features. +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# Based on the identifiers (e.g., 1007_s_at, 1053_at, etc.), these appear to be microarray probe IDs (Affymetrix), +# not standard human gene symbols. Therefore, mapping to gene symbols is required. +print("requires_gene_mapping = True") +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP: Gene Identifier Mapping + +# 1. Identify the probe column ("ID") and the gene symbol column ("Gene Symbol") in the gene annotation dataframe +probe_col = "ID" +gene_symbol_col = "Gene Symbol" + +# 2. Get the gene mapping dataframe +mapping_df = get_gene_mapping(gene_annotation, prob_col=probe_col, gene_col=gene_symbol_col) + +# 3. Apply the mapping to convert probe-level expression data into gene-level expression data +gene_data = apply_gene_mapping(gene_data, mapping_df) + +# Optional: Print a small subset to confirm +print("First 20 genes in the mapped gene_data index:") +print(gene_data.index[:20]) +print("Gene expression data shape:", gene_data.shape) +import os +import pandas as pd + +# STEP 7: Data Normalization and Linking + +# First, check if the clinical CSV file exists. If it does not, we cannot proceed with trait-based linking. +if not os.path.exists(out_clinical_data_file): + # No trait data file => dataset is not usable for trait analysis + df_null = pd.DataFrame() + is_biased = True # Arbitrary boolean to satisfy function requirement + validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=is_biased, + df=df_null, + note="No trait data file found; dataset not usable for trait analysis." + ) + +else: + # 1. Normalize the mapped gene expression data using known gene symbol synonyms, then save. + normalized_gene_data = normalize_gene_symbols_in_index(gene_data) + normalized_gene_data.to_csv(out_gene_data_file) + + # 2. Load the previously extracted clinical CSV. + selected_clinical_df = pd.read_csv(out_clinical_data_file) + # If we had a single-row trait, rename row 0 to the trait name (example usage). + selected_clinical_df = selected_clinical_df.rename(index={0: trait}) + + # Combine these as our final clinical data; in this dataset, we only have trait info (if any). + combined_clinical_df = selected_clinical_df + + # Link the clinical and genetic data by matching sample IDs in columns. + linked_data = geo_link_clinical_genetic_data(combined_clinical_df, normalized_gene_data) + + # 3. Handle missing values in the linked data (drop incomplete rows/columns, then impute). + processed_data = handle_missing_values(linked_data, trait) + + # 4. Check trait bias and remove any biased demographic features (if any). + trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + + # 5. Final validation and metadata saving. + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=processed_data, + note="Completed trait-based preprocessing." + ) + + # 6. If final dataset is usable, save. Otherwise, skip. + if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Metabolic_Rate/code/GSE41168.py b/p1/preprocess/Metabolic_Rate/code/GSE41168.py new file mode 100644 index 0000000000000000000000000000000000000000..146af15c11508a4ce7cd89bc8a1939d0556294a4 --- /dev/null +++ b/p1/preprocess/Metabolic_Rate/code/GSE41168.py @@ -0,0 +1,93 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Metabolic_Rate" +cohort = "GSE41168" + +# Input paths +in_trait_dir = "../DATA/GEO/Metabolic_Rate" +in_cohort_dir = "../DATA/GEO/Metabolic_Rate/GSE41168" + +# Output paths +out_data_file = "./output/preprocess/1/Metabolic_Rate/GSE41168.csv" +out_gene_data_file = "./output/preprocess/1/Metabolic_Rate/gene_data/GSE41168.csv" +out_clinical_data_file = "./output/preprocess/1/Metabolic_Rate/clinical_data/GSE41168.csv" +json_path = "./output/preprocess/1/Metabolic_Rate/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +import pandas as pd +from typing import Optional, Callable + +# 1. Gene expression data availability +is_gene_available = True # Based on the study design mentioning gene expression profiles +# 2. Variable availability +trait_row = None # No row key found for 'Metabolic_Rate' (trait data not present) +age_row = None # No row key for 'age' +gender_row = None # Although row 3 shows "gender: Female," it's constant across all samples; considered not available + +# 2.2 Data type conversion (functions are defined but not used since all rows are None) +def convert_trait(value: str) -> Optional[float]: + # Example: parse string after colon + parts = value.split(':') + if len(parts) > 1: + val = parts[1].strip().lower() + # Here we could convert to float, but no actual data is available + return None + return None + +def convert_age(value: str) -> Optional[float]: + # Example: parse string after colon + parts = value.split(':') + if len(parts) > 1: + val = parts[1].strip() + # Attempt conversion to float + try: + return float(val) + except ValueError: + return None + return None + +def convert_gender(value: str) -> Optional[int]: + # Example: parse string after colon + parts = value.split(':') + if len(parts) > 1: + val = parts[1].strip().lower() + if val == "male": + return 1 + elif val == "female": + return 0 + return None + +# 3. Initial filtering and metadata saving +is_trait_available = (trait_row is not None) # False since trait_row is None + +is_usable = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4. Clinical feature extraction (skip because trait_row is None) +if trait_row is not None: + # If we had trait data, we would extract and save clinical features here. + pass \ No newline at end of file diff --git a/p1/preprocess/Metabolic_Rate/code/GSE61225.py b/p1/preprocess/Metabolic_Rate/code/GSE61225.py new file mode 100644 index 0000000000000000000000000000000000000000..d458bf809ca4f8fdc17bf3684037932be503ea36 --- /dev/null +++ b/p1/preprocess/Metabolic_Rate/code/GSE61225.py @@ -0,0 +1,217 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Metabolic_Rate" +cohort = "GSE61225" + +# Input paths +in_trait_dir = "../DATA/GEO/Metabolic_Rate" +in_cohort_dir = "../DATA/GEO/Metabolic_Rate/GSE61225" + +# Output paths +out_data_file = "./output/preprocess/1/Metabolic_Rate/GSE61225.csv" +out_gene_data_file = "./output/preprocess/1/Metabolic_Rate/gene_data/GSE61225.csv" +out_clinical_data_file = "./output/preprocess/1/Metabolic_Rate/clinical_data/GSE61225.csv" +json_path = "./output/preprocess/1/Metabolic_Rate/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +import re + +# Step 1: Gene Expression Data Availability +# Based on the background info stating "Gene expression in whole blood RNA was evaluated using Illumina HumanHT-12v3..." +is_gene_available = True + +# Step 2: Variable Availability and Data Type Conversion + +# 2.1 Identify rows for trait, age, gender +# Looking at the sample characteristics: +# Row 4 -> "metabolic equivalents: ..." +# Row 5 -> "gender: female/male" +# Row 6 -> "age: " +trait_row = 4 # Metabolic_Rate corresponds to "metabolic equivalents" +age_row = 6 # Age is in row 6 +gender_row = 5 # Gender is in row 5 + +# 2.2 Define conversion functions + +def convert_trait(value: str): + """ + Convert the 'metabolic equivalents: ' string to a float. + Unknown or unparseable values return None. + """ + try: + # Extract text after the colon + val_str = value.split(":", 1)[1].strip() + return float(val_str) + except: + return None + +def convert_age(value: str): + """ + Convert the 'age: ' string to a float. + Unknown or unparseable values return None. + """ + try: + val_str = value.split(":", 1)[1].strip() + return float(val_str) + except: + return None + +def convert_gender(value: str): + """ + Convert 'gender: female' to 0, 'gender: male' to 1, otherwise None. + """ + try: + val_str = value.split(":", 1)[1].strip().lower() + if val_str == 'female': + return 0 + elif val_str == 'male': + return 1 + else: + return None + except: + return None + +# Determine if trait data is available +is_trait_available = (trait_row is not None) + +# Step 3: Save Metadata (initial filtering) +# Use validate_and_save_cohort_info with is_final=False +is_usable = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# Step 4: Clinical Feature Extraction (only if trait_row is not None) +if trait_row is not None: + # Suppose clinical_data is already obtained from the previous step as "clinical_data" + # but here we just assume it's in the variable name `clinical_data` + selected_features = geo_select_clinical_features( + clinical_df=clinical_data, + trait=trait, + trait_row=trait_row, + convert_trait=convert_trait, + age_row=age_row, + convert_age=convert_age, + gender_row=gender_row, + convert_gender=convert_gender + ) + + # Observe the output + preview = preview_df(selected_features) + print("Selected Clinical Features Preview:") + print(preview) + + # Save to CSV + selected_features.to_csv(out_clinical_data_file, index=False) +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# The ILMN_xxxx are Illumina probe IDs, so they are not standard human gene symbols. +# Hence, they require mapping to gene symbols. + +requires_gene_mapping = True +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP: Gene Identifier Mapping + +# 1. Identify the columns in 'gene_annotation' that match the probe IDs in 'gene_data' and store gene symbols. +# From the preview, 'ID' matches "ILMN_xxxx" in gene_data, and 'ILMN_Gene' stores the gene symbol. +probe_col = "ID" +symbol_col = "ILMN_Gene" + +# 2. Create the gene mapping dataframe +mapping_df = get_gene_mapping(annotation=gene_annotation, prob_col=probe_col, gene_col=symbol_col) + +# 3. Convert probe-level data to gene-level data +gene_data = apply_gene_mapping(expression_df=gene_data, mapping_df=mapping_df) + +# (Optional) Print the shape of the mapped gene_data for confirmation +print("Mapped gene_data shape:", gene_data.shape) +import os +import pandas as pd + +# STEP 7: Data Normalization and Linking + +# First, check if the clinical CSV file exists. If it does not, we cannot proceed with trait-based linking. +if not os.path.exists(out_clinical_data_file): + # No trait data file => dataset is not usable for trait analysis + df_null = pd.DataFrame() + is_biased = True # Arbitrary boolean to satisfy function requirement + validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=is_biased, + df=df_null, + note="No trait data file found; dataset not usable for trait analysis." + ) + +else: + # 1. Normalize the mapped gene expression data using known gene symbol synonyms, then save. + normalized_gene_data = normalize_gene_symbols_in_index(gene_data) + normalized_gene_data.to_csv(out_gene_data_file) + + # 2. Load the previously extracted clinical CSV. + selected_clinical_df = pd.read_csv(out_clinical_data_file) + # If we had a single-row trait, rename row 0 to the trait name (example usage). + selected_clinical_df = selected_clinical_df.rename(index={0: trait}) + + # Combine these as our final clinical data; in this dataset, we only have trait info (if any). + combined_clinical_df = selected_clinical_df + + # Link the clinical and genetic data by matching sample IDs in columns. + linked_data = geo_link_clinical_genetic_data(combined_clinical_df, normalized_gene_data) + + # 3. Handle missing values in the linked data (drop incomplete rows/columns, then impute). + processed_data = handle_missing_values(linked_data, trait) + + # 4. Check trait bias and remove any biased demographic features (if any). + trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + + # 5. Final validation and metadata saving. + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=processed_data, + note="Completed trait-based preprocessing." + ) + + # 6. If final dataset is usable, save. Otherwise, skip. + if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Metabolic_Rate/code/GSE89231.py b/p1/preprocess/Metabolic_Rate/code/GSE89231.py new file mode 100644 index 0000000000000000000000000000000000000000..375826da31eff1ed0611ffa687a59c098719bd56 --- /dev/null +++ b/p1/preprocess/Metabolic_Rate/code/GSE89231.py @@ -0,0 +1,182 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Metabolic_Rate" +cohort = "GSE89231" + +# Input paths +in_trait_dir = "../DATA/GEO/Metabolic_Rate" +in_cohort_dir = "../DATA/GEO/Metabolic_Rate/GSE89231" + +# Output paths +out_data_file = "./output/preprocess/1/Metabolic_Rate/GSE89231.csv" +out_gene_data_file = "./output/preprocess/1/Metabolic_Rate/gene_data/GSE89231.csv" +out_clinical_data_file = "./output/preprocess/1/Metabolic_Rate/clinical_data/GSE89231.csv" +json_path = "./output/preprocess/1/Metabolic_Rate/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1. Gene Expression Data Availability +is_gene_available = True # From the description: "Global gene expression data" + +# 2. Variable Availability +# Checking the sample characteristics dictionary, we only see cell line information. +# No mention of Metabolic_Rate (trait), age, or gender is given or can be inferred. +trait_row = None +age_row = None +gender_row = None + +# 2.2 Data Type Conversion Functions +def convert_trait(x: str) -> float: + """ + Convert the trait value (after the colon) to a floating number. + Unknown or invalid values -> None. + """ + parts = x.split(":") + val = parts[1].strip() if len(parts) > 1 else None + if not val or val.lower() in ["none", "na", "nan", "unknown"]: + return None + try: + return float(val) + except ValueError: + return None + +def convert_age(x: str) -> float: + """ + Convert the age value (after the colon) to a float. + Unknown or invalid values -> None. + """ + parts = x.split(":") + val = parts[1].strip() if len(parts) > 1 else None + if not val or val.lower() in ["none", "na", "nan", "unknown"]: + return None + try: + return float(val) + except ValueError: + return None + +def convert_gender(x: str) -> int: + """ + Convert the gender value (after the colon) to binary (female -> 0, male -> 1). + Unknown or invalid values -> None. + """ + parts = x.split(":") + val = parts[1].strip().lower() if len(parts) > 1 else None + if not val or val in ["none", "na", "nan", "unknown"]: + return None + if "female" in val: + return 0 + if "male" in val: + return 1 + return None + +# 3. Save Metadata with Initial Filtering +is_trait_available = (trait_row is not None) +is_usable = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4. Clinical Feature Extraction +# Only proceed if trait_row is not None (trait data is available). +# Here, trait_row is None, so skip. +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# These identifiers appear to be Affymetrix probe set IDs and not standard human gene symbols. +requires_gene_mapping = True +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP: Gene Identifier Mapping +# 1. Identify the columns in the gene annotation that correspond to the probe IDs (same identifiers as gene_data.index) +# and the gene symbols (the 'Gene Symbol' column). +mapping_df = get_gene_mapping(gene_annotation, prob_col="ID", gene_col="Gene Symbol") + +# 2. Convert probe-level measurements to gene expression data by applying the gene mapping. +gene_data = apply_gene_mapping(gene_data, mapping_df) +import os +import pandas as pd + +# STEP 7: Data Normalization and Linking + +# First, check if the clinical CSV file exists. If it does not, we cannot proceed with trait-based linking. +if not os.path.exists(out_clinical_data_file): + # No trait data file => dataset is not usable for trait analysis + df_null = pd.DataFrame() + is_biased = True # Arbitrary boolean to satisfy function requirement + validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=is_biased, + df=df_null, + note="No trait data file found; dataset not usable for trait analysis." + ) + +else: + # 1. Normalize the mapped gene expression data using known gene symbol synonyms, then save. + normalized_gene_data = normalize_gene_symbols_in_index(gene_data) + normalized_gene_data.to_csv(out_gene_data_file) + + # 2. Load the previously extracted clinical CSV. + selected_clinical_df = pd.read_csv(out_clinical_data_file) + # If we had a single-row trait, rename row 0 to the trait name (example usage). + selected_clinical_df = selected_clinical_df.rename(index={0: trait}) + + # Combine these as our final clinical data; in this dataset, we only have trait info (if any). + combined_clinical_df = selected_clinical_df + + # Link the clinical and genetic data by matching sample IDs in columns. + linked_data = geo_link_clinical_genetic_data(combined_clinical_df, normalized_gene_data) + + # 3. Handle missing values in the linked data (drop incomplete rows/columns, then impute). + processed_data = handle_missing_values(linked_data, trait) + + # 4. Check trait bias and remove any biased demographic features (if any). + trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + + # 5. Final validation and metadata saving. + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=processed_data, + note="Completed trait-based preprocessing." + ) + + # 6. If final dataset is usable, save. Otherwise, skip. + if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Metabolic_Rate/code/TCGA.py b/p1/preprocess/Metabolic_Rate/code/TCGA.py new file mode 100644 index 0000000000000000000000000000000000000000..8b86727e9eced4947b9786b317d3d4afc5a04b16 --- /dev/null +++ b/p1/preprocess/Metabolic_Rate/code/TCGA.py @@ -0,0 +1,70 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Metabolic_Rate" + +# Input paths +tcga_root_dir = "../DATA/TCGA" + +# Output paths +out_data_file = "./output/preprocess/1/Metabolic_Rate/TCGA.csv" +out_gene_data_file = "./output/preprocess/1/Metabolic_Rate/gene_data/TCGA.csv" +out_clinical_data_file = "./output/preprocess/1/Metabolic_Rate/clinical_data/TCGA.csv" +json_path = "./output/preprocess/1/Metabolic_Rate/cohort_info.json" + +import os +import pandas as pd + +# List of subdirectories provided in the instructions: +subdirectories = [ + 'CrawlData.ipynb', '.DS_Store', 'TCGA_lower_grade_glioma_and_glioblastoma_(GBMLGG)', + 'TCGA_Uterine_Carcinosarcoma_(UCS)', 'TCGA_Thyroid_Cancer_(THCA)', 'TCGA_Thymoma_(THYM)', + 'TCGA_Testicular_Cancer_(TGCT)', 'TCGA_Stomach_Cancer_(STAD)', 'TCGA_Sarcoma_(SARC)', + 'TCGA_Rectal_Cancer_(READ)', 'TCGA_Prostate_Cancer_(PRAD)', 'TCGA_Pheochromocytoma_Paraganglioma_(PCPG)', + 'TCGA_Pancreatic_Cancer_(PAAD)', 'TCGA_Ovarian_Cancer_(OV)', 'TCGA_Ocular_melanomas_(UVM)', + 'TCGA_Mesothelioma_(MESO)', 'TCGA_Melanoma_(SKCM)', 'TCGA_Lung_Squamous_Cell_Carcinoma_(LUSC)', + 'TCGA_Lung_Cancer_(LUNG)', 'TCGA_Lung_Adenocarcinoma_(LUAD)', 'TCGA_Lower_Grade_Glioma_(LGG)', + 'TCGA_Liver_Cancer_(LIHC)', 'TCGA_Large_Bcell_Lymphoma_(DLBC)', 'TCGA_Kidney_Papillary_Cell_Carcinoma_(KIRP)', + 'TCGA_Kidney_Clear_Cell_Carcinoma_(KIRC)', 'TCGA_Kidney_Chromophobe_(KICH)', 'TCGA_Head_and_Neck_Cancer_(HNSC)', + 'TCGA_Glioblastoma_(GBM)', 'TCGA_Esophageal_Cancer_(ESCA)', 'TCGA_Endometrioid_Cancer_(UCEC)', + 'TCGA_Colon_and_Rectal_Cancer_(COADREAD)', 'TCGA_Colon_Cancer_(COAD)', 'TCGA_Cervical_Cancer_(CESC)', + 'TCGA_Breast_Cancer_(BRCA)', 'TCGA_Bladder_Cancer_(BLCA)', 'TCGA_Bile_Duct_Cancer_(CHOL)', + 'TCGA_Adrenocortical_Cancer_(ACC)', 'TCGA_Acute_Myeloid_Leukemia_(LAML)' +] + +# Synonyms or keywords related to "Metabolic_Rate" +metab_synonyms = ["metab", "energy", "metabolic"] + +selected_subdirectory = None +for subdir in subdirectories: + subdir_lower = subdir.lower() + if any(syn in subdir_lower for syn in metab_synonyms): + selected_subdirectory = subdir + break + +if not selected_subdirectory: + # If no matching directory is found, mark dataset as unavailable + is_final = False + is_gene_available = False + is_trait_available = False + _ = validate_and_save_cohort_info( + is_final=is_final, + cohort="TCGA", + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available + ) + print(f"No suitable directory found for '{trait}'. Skipped this trait.") +else: + # Step 2: Identify clinicalMatrix file and PANCAN file + cohort_dir = os.path.join(tcga_root_dir, selected_subdirectory) + clinical_file_path, genetic_file_path = tcga_get_relevant_filepaths(cohort_dir) + + # Step 3: Load both files as dataframes + clinical_df = pd.read_csv(clinical_file_path, index_col=0, sep='\t') + genetic_df = pd.read_csv(genetic_file_path, index_col=0, sep='\t') + + # Step 4: Print the column names of the clinical data + print("Clinical data columns:") + print(list(clinical_df.columns)) \ No newline at end of file diff --git a/p1/preprocess/Metabolic_Rate/cohort_info.json b/p1/preprocess/Metabolic_Rate/cohort_info.json new file mode 100644 index 0000000000000000000000000000000000000000..29f603d092c9c54fbe25ca51879b06b25cef11a0 --- /dev/null +++ b/p1/preprocess/Metabolic_Rate/cohort_info.json @@ -0,0 +1 @@ +{"GSE89231": {"is_usable": false, "is_gene_available": false, "is_trait_available": false, "is_available": false, "is_biased": null, "has_age": null, "has_gender": null, "sample_size": null, "note": "No trait data file found; dataset not usable for trait analysis."}, "GSE61225": {"is_usable": true, "is_gene_available": true, "is_trait_available": true, "is_available": true, "is_biased": false, "has_age": false, "has_gender": false, "sample_size": 74, "note": "Completed trait-based preprocessing."}, "GSE41168": {"is_usable": false, "is_gene_available": true, "is_trait_available": false, "is_available": false, "is_biased": null, "has_age": null, "has_gender": null, "sample_size": null, "note": null}, "GSE40873": {"is_usable": false, "is_gene_available": false, "is_trait_available": false, "is_available": false, "is_biased": null, "has_age": null, "has_gender": null, "sample_size": null, "note": "No trait data file found; dataset not usable for trait analysis."}, "GSE40589": {"is_usable": false, "is_gene_available": false, "is_trait_available": false, "is_available": false, "is_biased": null, "has_age": null, "has_gender": null, "sample_size": null, "note": "No trait data file found; dataset not usable for trait analysis."}, "GSE26440": {"is_usable": false, "is_gene_available": false, "is_trait_available": false, "is_available": false, "is_biased": null, "has_age": null, "has_gender": null, "sample_size": null, "note": "No trait data file found; dataset not usable for trait analysis."}, "GSE23025": {"is_usable": false, "is_gene_available": false, "is_trait_available": false, "is_available": false, "is_biased": null, "has_age": null, "has_gender": null, "sample_size": null, "note": "No trait data file found; dataset not usable for trait analysis."}, "GSE151683": {"is_usable": false, "is_gene_available": false, "is_trait_available": false, "is_available": false, "is_biased": null, "has_age": null, "has_gender": null, "sample_size": null, "note": "No trait data file found; dataset not usable for trait analysis."}, "GSE106800": {"is_usable": false, "is_gene_available": false, "is_trait_available": false, "is_available": false, "is_biased": null, "has_age": null, "has_gender": null, "sample_size": null, "note": "No trait data file found; dataset not usable for trait analysis."}, "GSE101492": {"is_usable": true, "is_gene_available": true, "is_trait_available": true, "is_available": true, "is_biased": false, "has_age": false, "has_gender": false, "sample_size": 80, "note": "Completed trait-based preprocessing."}, "TCGA": {"is_usable": false, "is_gene_available": false, "is_trait_available": false, "is_available": false, "is_biased": null, "has_age": null, "has_gender": null, "sample_size": null, "note": null}} \ No newline at end of file diff --git a/p1/preprocess/Migraine/clinical_data/GSE67311.csv b/p1/preprocess/Migraine/clinical_data/GSE67311.csv new file mode 100644 index 0000000000000000000000000000000000000000..ef7bf1bf5002b546e705c8534b32ea1daa665328 --- /dev/null +++ b/p1/preprocess/Migraine/clinical_data/GSE67311.csv @@ -0,0 +1,2 @@ +GSM1644447,GSM1644448,GSM1644449,GSM1644450,GSM1644451,GSM1644452,GSM1644453,GSM1644454,GSM1644455,GSM1644456,GSM1644457,GSM1644458,GSM1644459,GSM1644460,GSM1644461,GSM1644462,GSM1644463,GSM1644464,GSM1644465,GSM1644466,GSM1644467,GSM1644468,GSM1644469,GSM1644470,GSM1644471,GSM1644472,GSM1644473,GSM1644474,GSM1644475,GSM1644476,GSM1644477,GSM1644478,GSM1644479,GSM1644480,GSM1644481,GSM1644482,GSM1644483,GSM1644484,GSM1644485,GSM1644486,GSM1644487,GSM1644488,GSM1644489,GSM1644490,GSM1644491,GSM1644492,GSM1644493,GSM1644494,GSM1644495,GSM1644496,GSM1644497,GSM1644498,GSM1644499,GSM1644500,GSM1644501,GSM1644502,GSM1644503,GSM1644504,GSM1644505,GSM1644506,GSM1644507,GSM1644508,GSM1644509,GSM1644510,GSM1644511,GSM1644512,GSM1644513,GSM1644514,GSM1644515,GSM1644516,GSM1644517,GSM1644518,GSM1644519,GSM1644520,GSM1644521,GSM1644522,GSM1644523,GSM1644524,GSM1644525,GSM1644526,GSM1644527,GSM1644528,GSM1644529,GSM1644530,GSM1644531,GSM1644532,GSM1644533,GSM1644534,GSM1644535,GSM1644536,GSM1644537,GSM1644538,GSM1644539,GSM1644540,GSM1644541,GSM1644542,GSM1644543,GSM1644544,GSM1644545,GSM1644546,GSM1644547,GSM1644548,GSM1644549,GSM1644550,GSM1644551,GSM1644552,GSM1644553,GSM1644554,GSM1644555,GSM1644556,GSM1644557,GSM1644558,GSM1644559,GSM1644560,GSM1644561,GSM1644562,GSM1644563,GSM1644564,GSM1644565,GSM1644566,GSM1644567,GSM1644568,GSM1644569,GSM1644570,GSM1644571,GSM1644572,GSM1644573,GSM1644574,GSM1644575,GSM1644576,GSM1644577,GSM1644578,GSM1644579,GSM1644580,GSM1644581,GSM1644582,GSM1644583,GSM1644584,GSM1644585,GSM1644586,GSM1644587,GSM1644588 +0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,1.0,1.0,0.0,1.0,0.0,0.0,0.0,1.0,0.0,1.0,0.0,0.0,0.0,0.0,0.0,0.0,1.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,,0.0,,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,1.0,0.0,0.0,1.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,,0.0,0.0,0.0,0.0,0.0,0.0,0.0,1.0,0.0,1.0,0.0,1.0,0.0,1.0,1.0,1.0,1.0,1.0,0.0,0.0,1.0,,1.0,1.0,1.0,1.0,0.0,1.0,,1.0,,1.0,1.0,0.0,0.0,,0.0,1.0,0.0,1.0,0.0,0.0,0.0,,0.0,1.0,0.0,0.0,1.0,0.0,0.0,1.0,0.0,1.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,1.0,0.0,1.0,1.0,0.0,1.0,1.0,,1.0,1.0,0.0 diff --git a/p1/preprocess/Migraine/code/GSE67311.py b/p1/preprocess/Migraine/code/GSE67311.py new file mode 100644 index 0000000000000000000000000000000000000000..9d38bedcebc1c950add628f104948efdc19bea01 --- /dev/null +++ b/p1/preprocess/Migraine/code/GSE67311.py @@ -0,0 +1,184 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Migraine" +cohort = "GSE67311" + +# Input paths +in_trait_dir = "../DATA/GEO/Migraine" +in_cohort_dir = "../DATA/GEO/Migraine/GSE67311" + +# Output paths +out_data_file = "./output/preprocess/1/Migraine/GSE67311.csv" +out_gene_data_file = "./output/preprocess/1/Migraine/gene_data/GSE67311.csv" +out_clinical_data_file = "./output/preprocess/1/Migraine/clinical_data/GSE67311.csv" +json_path = "./output/preprocess/1/Migraine/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1. Gene Expression Data Availability +is_gene_available = True # Based on the background info (Affymetrix arrays, RMA normalization) +# 2.1 Identify trait_row, age_row, and gender_row +# After examining the sample characteristics and the background, we see: +# - For Migraine, row 4 has values: 'migraine: No', 'migraine: Yes', 'migraine: -' +# -> Multiple unique values, so trait data is available at key = 4. +# - No row indicates age data. +# - No row indicates gender data. +trait_row = 4 +age_row = None +gender_row = None + +# 2.2 Data Type Conversion +def convert_trait(x: str): + # Extract the substring after the colon + val = x.split(":", 1)[-1].strip().lower() if isinstance(x, str) else "" + # Convert to binary + if val == "yes": + return 1 + elif val == "no": + return 0 + else: + return None + +def convert_age(x: str): + # Not available in this dataset + return None + +def convert_gender(x: str): + # Not available in this dataset + return None + +# 3. Save Metadata (initial filtering) +is_trait_available = (trait_row is not None) +is_usable = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4. Clinical Feature Extraction +if trait_row is not None: + selected_clinical_df = geo_select_clinical_features( + clinical_data, # Assuming 'clinical_data' DataFrame is available + trait=trait, + trait_row=trait_row, + convert_trait=convert_trait, + age_row=age_row, + convert_age=convert_age, + gender_row=gender_row, + convert_gender=convert_gender + ) + # Preview and save + print("Preview of selected clinical features:") + preview_result = preview_df(selected_clinical_df) + print(preview_result) + selected_clinical_df.to_csv(out_clinical_data_file, index=False) +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# After reviewing the given identifiers (e.g., '7892501'), they appear to be probe IDs or other IDs, not standard human gene symbols. +# Therefore, they likely need to be mapped to gene symbols. +print("requires_gene_mapping = True") +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP6: Gene Identifier Mapping + +# 1. Determine the columns in the annotation DataFrame that store the probe IDs (matching gene_data.index) +# and the gene symbols. +# From the preview, "ID" holds the probe identifiers and "gene_assignment" contains gene symbols. +prob_col = "ID" +gene_col = "gene_assignment" + +# 2. Get a gene mapping DataFrame for converting from probe IDs to gene symbols +mapping_df = get_gene_mapping(gene_annotation, prob_col=prob_col, gene_col=gene_col) + +# 3. Convert probe-level measurements to gene expression data using the many-to-many mapping rules +gene_data = apply_gene_mapping(gene_data, mapping_df) + +# For verification, print the shape of the resulting DataFrame +print("Mapped gene_data shape:", gene_data.shape) +import os +import pandas as pd + +# STEP 7: Data Normalization and Linking + +# First, check if the clinical CSV file exists. If it does not, we cannot proceed with trait-based linking. +if not os.path.exists(out_clinical_data_file): + # No trait data file => dataset is not usable for trait analysis + df_null = pd.DataFrame() + is_biased = True # Arbitrary boolean to satisfy function requirement + validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=is_biased, + df=df_null, + note="No trait data file found; dataset not usable for trait analysis." + ) + +else: + # 1. Normalize the mapped gene expression data using known gene symbol synonyms, then save. + normalized_gene_data = normalize_gene_symbols_in_index(gene_data) + normalized_gene_data.to_csv(out_gene_data_file) + + # 2. Load the previously extracted clinical CSV. + selected_clinical_df = pd.read_csv(out_clinical_data_file) + # If we had a single-row trait, rename row 0 to the trait name (example usage). + selected_clinical_df = selected_clinical_df.rename(index={0: trait}) + + # Combine these as our final clinical data; in this dataset, we only have trait info (if any). + combined_clinical_df = selected_clinical_df + + # Link the clinical and genetic data by matching sample IDs in columns. + linked_data = geo_link_clinical_genetic_data(combined_clinical_df, normalized_gene_data) + + # 3. Handle missing values in the linked data (drop incomplete rows/columns, then impute). + processed_data = handle_missing_values(linked_data, trait) + + # 4. Check trait bias and remove any biased demographic features (if any). + trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + + # 5. Final validation and metadata saving. + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=processed_data, + note="Completed trait-based preprocessing." + ) + + # 6. If final dataset is usable, save. Otherwise, skip. + if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Migraine/code/TCGA.py b/p1/preprocess/Migraine/code/TCGA.py new file mode 100644 index 0000000000000000000000000000000000000000..552c6b7b35c09b3a3ebb848d5914119dcb61591f --- /dev/null +++ b/p1/preprocess/Migraine/code/TCGA.py @@ -0,0 +1,70 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Migraine" + +# Input paths +tcga_root_dir = "../DATA/TCGA" + +# Output paths +out_data_file = "./output/preprocess/1/Migraine/TCGA.csv" +out_gene_data_file = "./output/preprocess/1/Migraine/gene_data/TCGA.csv" +out_clinical_data_file = "./output/preprocess/1/Migraine/clinical_data/TCGA.csv" +json_path = "./output/preprocess/1/Migraine/cohort_info.json" + +import os +import pandas as pd + +# List of subdirectories provided in the instructions: +subdirectories = [ + 'CrawlData.ipynb', '.DS_Store', 'TCGA_lower_grade_glioma_and_glioblastoma_(GBMLGG)', + 'TCGA_Uterine_Carcinosarcoma_(UCS)', 'TCGA_Thyroid_Cancer_(THCA)', 'TCGA_Thymoma_(THYM)', + 'TCGA_Testicular_Cancer_(TGCT)', 'TCGA_Stomach_Cancer_(STAD)', 'TCGA_Sarcoma_(SARC)', + 'TCGA_Rectal_Cancer_(READ)', 'TCGA_Prostate_Cancer_(PRAD)', 'TCGA_Pheochromocytoma_Paraganglioma_(PCPG)', + 'TCGA_Pancreatic_Cancer_(PAAD)', 'TCGA_Ovarian_Cancer_(OV)', 'TCGA_Ocular_melanomas_(UVM)', + 'TCGA_Mesothelioma_(MESO)', 'TCGA_Melanoma_(SKCM)', 'TCGA_Lung_Squamous_Cell_Carcinoma_(LUSC)', + 'TCGA_Lung_Cancer_(LUNG)', 'TCGA_Lung_Adenocarcinoma_(LUAD)', 'TCGA_Lower_Grade_Glioma_(LGG)', + 'TCGA_Liver_Cancer_(LIHC)', 'TCGA_Large_Bcell_Lymphoma_(DLBC)', 'TCGA_Kidney_Papillary_Cell_Carcinoma_(KIRP)', + 'TCGA_Kidney_Clear_Cell_Carcinoma_(KIRC)', 'TCGA_Kidney_Chromophobe_(KICH)', 'TCGA_Head_and_Neck_Cancer_(HNSC)', + 'TCGA_Glioblastoma_(GBM)', 'TCGA_Esophageal_Cancer_(ESCA)', 'TCGA_Endometrioid_Cancer_(UCEC)', + 'TCGA_Colon_and_Rectal_Cancer_(COADREAD)', 'TCGA_Colon_Cancer_(COAD)', 'TCGA_Cervical_Cancer_(CESC)', + 'TCGA_Breast_Cancer_(BRCA)', 'TCGA_Bladder_Cancer_(BLCA)', 'TCGA_Bile_Duct_Cancer_(CHOL)', + 'TCGA_Adrenocortical_Cancer_(ACC)', 'TCGA_Acute_Myeloid_Leukemia_(LAML)' +] + +# Synonyms or keywords related to "Migraine" +migraine_synonyms = ["migraine", "headache"] + +selected_subdirectory = None +for subdir in subdirectories: + subdir_lower = subdir.lower() + if any(syn in subdir_lower for syn in migraine_synonyms): + selected_subdirectory = subdir + break + +if not selected_subdirectory: + # If no matching directory is found, mark dataset as unavailable + is_final = False + is_gene_available = False + is_trait_available = False + _ = validate_and_save_cohort_info( + is_final=is_final, + cohort="TCGA", + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available + ) + print(f"No suitable directory found for '{trait}'. Skipped this trait.") +else: + # Step 2: Identify clinicalMatrix file and PANCAN file + cohort_dir = os.path.join(tcga_root_dir, selected_subdirectory) + clinical_file_path, genetic_file_path = tcga_get_relevant_filepaths(cohort_dir) + + # Step 3: Load both files as dataframes + clinical_df = pd.read_csv(clinical_file_path, index_col=0, sep='\t') + genetic_df = pd.read_csv(genetic_file_path, index_col=0, sep='\t') + + # Step 4: Print the column names of the clinical data + print("Clinical data columns:") + print(list(clinical_df.columns)) \ No newline at end of file diff --git a/p1/preprocess/Migraine/cohort_info.json b/p1/preprocess/Migraine/cohort_info.json new file mode 100644 index 0000000000000000000000000000000000000000..0a72e629a3998d724040774d97ab60c46ac05e0c --- /dev/null +++ b/p1/preprocess/Migraine/cohort_info.json @@ -0,0 +1 @@ +{"GSE67311": {"is_usable": true, "is_gene_available": true, "is_trait_available": true, "is_available": true, "is_biased": false, "has_age": false, "has_gender": false, "sample_size": 133, "note": "Completed trait-based preprocessing."}, "TCGA": {"is_usable": false, "is_gene_available": false, "is_trait_available": false, "is_available": false, "is_biased": null, "has_age": null, "has_gender": null, "sample_size": null, "note": null}} \ No newline at end of file diff --git a/p1/preprocess/Mitochondrial_Disorders/GSE42986.csv b/p1/preprocess/Mitochondrial_Disorders/GSE42986.csv new file mode 100644 index 0000000000000000000000000000000000000000..5058d968f30cd77abd378560a9b83016c0d3e0a4 --- /dev/null +++ b/p1/preprocess/Mitochondrial_Disorders/GSE42986.csv @@ -0,0 +1,44 @@ 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KS4B,ANKS6,ANKUB1,ANKZF1,ANLN,ANO1,ANO10,ANO2,ANO3,ANO4,ANO5,ANO6,ANO7,ANO8,ANO9,ANOS1,ANP32A,ANP32A-IT1,ANP32B,ANP32CP,ANP32D,ANP32E,ANPEP,ANTKMT,ANTXR1,ANTXR2,ANTXRL,ANXA1,ANXA10,ANXA11,ANXA13,ANXA2,ANXA2P1,ANXA2P2,ANXA2P3,ANXA2R,ANXA3,ANXA4,ANXA5,ANXA6,ANXA7,ANXA8,ANXA8L1,ANXA9,AOAH,AOC2,AOC3,AOC4P,AOPEP,AOX1,AOX2P,AP1AR,AP1B1,AP1G1,AP1G2,AP1M1,AP1M2,AP1S1,AP1S2,AP1S3,AP2A1,AP2A2,AP2B1,AP2M1,AP2S1,AP3B1,AP3B2,AP3D1,AP3M1,AP3M2,AP3S1,AP3S2,AP4B1,AP4E1,AP4M1,AP4S1,AP5M1,AP5S1,AP5Z1,APAF1,APBA1,APBA2,APBA3,APBB1,APBB1IP,APBB2,APBB3,APC,APC2,APCDD1,APCDD1L,APCS,APEH,APEX1,APEX2,APH1A,APH1B,API5,APIP,APLF,APLN,APLNR,APLP1,APLP2,APMAP,APOA1,APOA2,APOA4,APOA5,APOB,APOBEC1,APOBEC2,APOBEC3A,APOBEC3B,APOBEC3C,APOBEC3D,APOBEC3F,APOBEC3G,APOBEC3H,APOBEC4,APOBR,APOC1,APOC1P1,APOC2,APOC3,APOC4,APOD,APOE,APOF,APOH,APOL1,APOL2,APOL3,APOL4,APOL5,APOL6,APOLD1,APOO,APOOL,APP,APPBP2,APPL1,APPL2,APRG1,APRT,APTX,AQP1,AQP10,AQP11,AQP12A,AQP12B,AQP2,AQP3,AQP4,AQP4-AS1,AQP5,AQP6,AQP7,AQP7P1,AQP8,AQP9,AQR,AR,ARAF,ARAP1,ARAP2,ARAP3,ARB2A,ARB2BP,ARC,ARCN1,AREG,AREL1,ARF1,ARF3,ARF4,ARF5,ARF6,ARFGAP1,ARFGAP2,ARFGAP3,ARFGEF1,ARFGEF2,ARFGEF3,ARFIP1,ARFIP2,ARFRP1,ARG1,ARG2,ARGFX,ARGFXP2,ARGLU1,ARHGAP1,ARHGAP10,ARHGAP11A,ARHGAP11B,ARHGAP12,ARHGAP15,ARHGAP17,ARHGAP18,ARHGAP19,ARHGAP20,ARHGAP21,ARHGAP22,ARHGAP23,ARHGAP24,ARHGAP25,ARHGAP26,ARHGAP27,ARHGAP28,ARHGAP29,ARHGAP30,ARHGAP31,ARHGAP32,ARHGAP33,ARHGAP35,ARHGAP36,ARHGAP39,ARHGAP4,ARHGAP40,ARHGAP42,ARHGAP44,ARHGAP45,ARHGAP5,ARHGAP5-AS1,ARHGAP6,ARHGAP8,ARHGAP9,ARHGDIA,ARHGDIB,ARHGDIG,ARHGEF1,ARHGEF10,ARHGEF10L,ARHGEF11,ARHGEF12,ARHGEF15,ARHGEF16,ARHGEF17,ARHGEF18,ARHGEF19,ARHGEF2,ARHGEF25,ARHGEF26,ARHGEF28,ARHGEF3,ARHGEF33,ARHGEF35,ARHGEF37,ARHGEF38,ARHGEF39,ARHGEF4,ARHGEF40,ARHGEF5,ARHGEF6,ARHGEF7,ARHGEF9,ARID1A,ARID1B,ARID2,ARID3A,ARID3B,ARID3C,ARID4A,ARID4B,ARID5A,ARID5B,ARIH1,ARIH2,ARIH2OS,ARK2C,ARK2N,ARL1,ARL10,ARL11,ARL13A,ARL13B,ARL14,ARL14EP,ARL15,ARL16,ARL2,ARL2-SNX15,ARL2BP,ARL3,ARL4A,ARL4C,ARL4D,ARL5A,ARL5B,ARL5C,ARL6,ARL6IP1,ARL6IP4,ARL6IP5,ARL6IP6,ARL8A,ARL8B,ARL9,ARLN,ARMC1,ARMC10,ARMC12,ARMC2,ARMC3,ARMC5,ARMC6,ARMC7,ARMC8,ARMC9,ARMCX1,ARMCX2,ARMCX3,ARMCX4,ARMCX5,ARMCX5-GPRASP2,ARMCX6,ARMH1,ARMH3,ARMH4,ARMS2,ARMT1,ARNT,ARNT2,ARPC1A,ARPC1B,ARPC2,ARPC3,ARPC4,ARPC5,ARPC5L,ARPIN-AP3S2,ARPP19,ARPP21,ARR3,ARRB1,ARRB2,ARRDC1,ARRDC1-AS1,ARRDC2,ARRDC3,ARRDC4,ARRDC5,ARSA,ARSB,ARSD,ARSF,ARSG,ARSH,ARSI,ARSJ,ARSK,ARSL,ART1,ART3,ART4,ART5,ARTN,ARV1,ARVCF,ARX,AS3MT,ASAH1,ASAH2,ASAH2B,ASAP1,ASAP1-IT1,ASAP2,ASAP3,ASB1,ASB10,ASB11,ASB12,ASB13,ASB14,ASB15,ASB16,ASB16-AS1,ASB17,ASB18,ASB2,ASB3,ASB4,ASB5,ASB6,ASB7,ASB8,ASB9,ASB9P1,ASCC1,ASCC2,ASCC3,ASCL1,ASCL2,ASCL3,ASCL4,ASF1A,ASF1B,ASGR1,ASGR2,ASH1L,ASH2L,ASIC1,ASIC2,ASIC3,ASIC4,ASIC5,ASIP,ASL,ASNS,ASNSD1,ASPA,ASPDH,ASPG,ASPH,ASPHD1,ASPHD2,ASPM,ASPN,ASPRV1,ASPSCR1,ASRGL1,ASS1,ASTE1,ASTL,ASTN1,ASTN2,ASXL1,ASXL2,ASXL3,ASZ1,ATAD1,ATAD2,ATAD2B,ATAD3A,ATAD3B,ATAD3C,ATAD5,ATCAY,ATE1,ATF1,ATF2,ATF3,ATF4,ATF5,ATF6,ATF7,ATF7IP,ATF7IP2,ATG10,ATG101,ATG12,ATG13,ATG14,ATG16L1,ATG16L2,ATG2A,ATG2B,ATG3,ATG4A,ATG4B,ATG4C,ATG4D,ATG5,ATG7,ATG9A,ATG9B,ATIC,ATL1,ATL2,ATL3,ATM,ATMIN,ATN1,ATOH1,ATOH7,ATOH8,ATOSA,ATOSB,ATOX1,ATP10A,ATP10B,ATP10D,ATP11A,ATP11AUN,ATP11B,ATP11C,ATP12A,ATP13A1,ATP13A2,ATP13A3,ATP13A4,ATP13A5,ATP1A1,ATP1A1-AS1,ATP1A2,ATP1A3,ATP1A4,ATP1B1,ATP1B2,ATP1B3,ATP1B4,ATP23,ATP2A1,ATP2A2,ATP2A3,ATP2B1,ATP2B2,ATP2B3,ATP2B4,ATP2C1,ATP2C2,ATP4A,ATP4B,ATP5F1A,ATP5F1B,ATP5F1C,ATP5F1D,ATP5F1E,ATP5F1EP2,ATP5IF1,ATP5MC1,ATP5MC2,ATP5MC3,ATP5ME,ATP5MF,ATP5MG,ATP5MGL,ATP5MJ,ATP5MK,ATP5PB,ATP5PD,ATP5PF,ATP5PO,ATP6AP1,ATP6AP1L,ATP6AP2,ATP6V0A1,ATP6V0A2,ATP6V0A4,ATP6V0B,ATP6V0C,ATP6V0D1,ATP6V0D2,ATP6V0E1,ATP6V0E2,ATP6V1A,ATP6V1B1,ATP6V1B2,ATP6V1C1,ATP6V1C2,ATP6V1D,ATP6V1E1,ATP6V1E2,ATP6V1F,ATP6V1G1,ATP6V1G3,ATP6V1H,ATP7A,ATP7B,ATP8A1,ATP8A2,ATP8B1,ATP8B2,ATP8B3,ATP8B4,ATP8B5P,ATP9A,ATP9B,ATPAF1,ATPAF2,ATPSCKMT,ATR,ATRAID,ATRIP,ATRN,ATRNL1,ATRX,ATXN1,ATXN10,ATXN1L,ATXN2,ATXN2L,ATXN3,ATXN3L,ATXN7,ATXN7L1,ATXN7L2,ATXN7L3,ATXN7L3B,ATXN8OS,AUH,AUNIP,AUP1,AURKA,AURKAIP1,AURKAP1,AURKB,AURKC,AUTS2,AVEN,AVIL,AVL9,AVP,AVPI1,AVPR1A,AVPR1B,AVPR2,AWAT1,AWAT2,AXDND1,AXIN1,AXIN2,AXL,AZGP1,AZGP1P1,AZI2,AZIN1,AZU1,B2M,B3GALNT1,B3GALNT2,B3GALT1,B3GALT2,B3GALT5,B3GALT5-AS1,B3GALT6,B3GAT1,B3GAT2,B3GAT3,B3GLCT,B3GNT2,B3GNT3,B3GNT4,B3GNT5,B3GNT6,B3GNT7,B3GNT8,B3GNT9,B3GNTL1,B4GALNT1,B4GALNT2,B4GALNT3,B4GALNT4,B4GALT1,B4GALT2,B4GALT3,B4GALT4,B4GALT5,B4GALT6,B4GALT7,B4GAT1,B9D1,B9D2,BAALC,BAALC-AS2,BAAT,BABAM1,BABAM2,BACE1,BACE2,BACH1,BACH2,BAD,BAG1,BAG2,BAG3,BAG4,BAG5,BAGE,BAGE2,BAGE3,BAGE4,BAGE5,BAHCC1,BAHD1,BAIAP2,BAIAP2L1,BAIAP2L2,BAIAP3,BAK1,BAMBI,BANF1,BANF2,BANK1,BANP,BAP1,BARD1,BARHL1,BARHL2,BARX1,BARX2,BASP1,BASP1P1,BATF,BATF2,BATF3,BAX,BAZ1A,BAZ1B,BAZ2A,BAZ2B,BBC3,BBIP1,BBLN,BBOF1,BBOX1,BBS1,BBS10,BBS12,BBS2,BBS4,BBS5,BBS7,BBS9,BBX,BCAM,BCAN,BCAP29,BCAP31,BCAR1,BCAR3,BCAR4,BCAS1,BCAS2,BCAS3,BCAS4,BCAT1,BCAT2,BCCIP,BCDIN3D,BCHE,BCKDHA,BCKDHB,BCKDK,BCL10,BCL11A,BCL11B,BCL2,BCL2A1,BCL2L1,BCL2L10,BCL2L11,BCL2L12,BCL2L13,BCL2L14,BCL2L15,BCL2L2,BCL3,BCL6,BCL6B,BCL7A,BCL7B,BCL7C,BCL9,BCL9L,BCLAF1,BCO1,BCO2,BCOR,BCORL1,BCORP1,BCR,BCRP2,BCRP3,BCS1L,BDH1,BDH2,BDKRB1,BDKRB2,BDNF,BDNF-AS,BDP1,BEAN1,BECN1,BEGAIN,BEND2,BEND3,BEND4,BEND5,BEND6,BEND7,BEST1,BEST2,BEST3,BEST4,BET1,BET1L,BEX1,BEX2,BEX3,BEX4,BEX5,BFAR,BFSP1,BFSP2,BGLAP,BGN,BHLHA15,BHLHA9,BHLHE22,BHLHE23,BHLHE40,BHLHE41,BHMT,BHMT2,BICC1,BICD1,BICD2,BICDL1,BICDL2,BICDL3P,BICRA,BICRAL,BID,BIK,BIN1,BIN2,BIN3,BIRC2,BIRC3,BIRC5,BIRC6,BIRC7,BIRC8,BIVM,BLCAP,BLID,BLK,BLM,BLMH,BLNK,BLOC1S1,BLOC1S2,BLOC1S3,BLOC1S4,BLOC1S5,BLOC1S6,BLTP1,BLTP2,BLTP3A,BLTP3B,BLVRA,BLVRB,BLZF1,BMAL1,BMAL2,BMERB1,BMF,BMI1,BMP1,BMP10,BMP15,BMP2,BMP2K,BMP3,BMP4,BMP5,BMP6,BMP7,BMP8A,BMP8B,BMPER,BMPR1A,BMPR1B,BMPR2,BMS1,BMS1P4,BMX,BNC1,BNC2,BNIP1,BNIP2,BNIP3,BNIP3L,BNIP5,BNIPL,BOC,BOD1,BOD1L1,BOD1L2,BOK,BOK-AS1,BOLA1,BOLA3,BOLL,BOP1,BORA,BORCS5,BORCS6,BORCS7,BPESC1,BPGM,BPHL,BPI,BPIFA1,BPIFA2,BPIFA3,BPIFA4P,BPIFB1,BPIFB2,BPIFB3,BPIFB4,BPIFB6,BPIFC,BPNT1,BPNT2,BPTF,BRAF,BRAP,BRAT1,BRCA1,BRCA2,BRCC3,BRD1,BRD10,BRD2,BRD3,BRD3OS,BRD4,BRD7,BRD7P3,BRD8,BRD9,BRDT,BRF1,BRF2,BRI3,BRI3BP,BRICD5,BRINP2,BRINP3,BRIP1,BRIX1,BRK1,BRME1,BRMS1,BRMS1L,BROX,BRPF1,BRPF3,BRS3,BRSK1,BRSK2,BRWD1,BRWD1-AS2,BRWD3,BSCL2,BSDC1,BSG,BSN,BSND,BSPH1,BSPRY,BST1,BST2,BSX,BTAF1,BTBD1,BTBD10,BTBD16,BTBD17,BTBD18,BTBD19,BTBD2,BTBD3,BTBD6,BTBD7,BTBD8,BTBD9,BTC,BTD,BTF3,BTF3L4,BTF3P11,BTG1,BTG2,BTG3,BTG4,BTK,BTLA,BTN1A1,BTN2A1,BTN2A2,BTN2A3P,BTN3A1,BTN3A2,BTN3A3,BTNL3,BTNL8,BTNL9,BTRC,BUB1,BUB1B,BUB3,BUD13,BUD23,BUD31,BYSL,BZW1,BZW2,C10orf105,C10orf120,C10orf53,C10orf55,C10orf62,C10orf67,C10orf71,C10orf88,C10orf90,C10orf95,C11orf16,C11orf21,C11orf24,C11orf40,C11orf42,C11orf54,C11orf58,C11orf65,C11orf68,C11orf71,C11orf86,C11orf87,C11orf91,C11orf96,C11orf98,C12orf42,C12orf43,C12orf50,C12orf54,C12orf56,C12orf57,C12orf60,C12orf71,C12orf75,C12orf76,C14orf119,C14orf132,C14orf180,C14orf39,C14orf93,C15orf32,C15orf39,C15orf40,C15orf48,C15orf61,C15orf62,C16orf46,C16orf54,C16orf74,C16orf78,C16orf82,C16orf86,C16orf87,C16orf89,C16orf90,C16orf92,C17orf100,C17orf107,C17orf50,C17orf58,C17orf67,C17orf75,C17orf78,C17orf99,C18orf21,C18orf32,C18orf54,C19orf12,C19orf18,C19orf25,C19orf33,C19orf38,C19orf44,C19orf47,C19orf48P,C19orf53,C19orf73,C1D,C1GALT1,C1GALT1C1,C1QA,C1QB,C1QBP,C1QC,C1QL1,C1QL2,C1QL3,C1QL4,C1QTNF1,C1QTNF12,C1QTNF2,C1QTNF3,C1QTNF4,C1QTNF5,C1QTNF6,C1QTNF7,C1QTNF8,C1QTNF9,C1QTNF9B,C1R,C1RL,C1S,C1orf105,C1orf115,C1orf116,C1orf122,C1orf141,C1orf146,C1orf159,C1orf162,C1orf174,C1orf185,C1orf198,C1orf21,C1orf210,C1orf216,C1orf220,C1orf226,C1orf35,C1orf43,C1orf50,C1orf52,C1orf53,C1orf54,C1orf56,C1orf74,C1orf87,C1orf94,C2,C20orf141,C20orf144,C20orf173,C20orf202,C20orf203,C20orf204,C20orf96,C21orf58,C21orf91,C22orf15,C22orf23,C22orf31,C22orf39,C22orf42,C22orf46P,C2CD2,C2CD2L,C2CD3,C2CD4A,C2CD4B,C2CD4C,C2CD4D,C2CD5,C2CD6,C2orf15,C2orf42,C2orf49,C2orf66,C2orf68,C2orf69,C2orf72,C2orf74,C2orf76,C2orf78,C2orf80,C2orf81,C2orf88,C3,C3AR1,C3P1,C3orf18,C3orf20,C3orf22,C3orf33,C3orf36,C3orf38,C3orf49,C3orf52,C3orf62,C3orf70,C4BPA,C4BPB,C4orf17,C4orf33,C4orf36,C4orf46,C4orf51,C5,C5AR1,C5AR2,C5orf15,C5orf22,C5orf24,C5orf34,C5orf46,C5orf47,C5orf52,C5orf58,C6,C6orf118,C6orf120,C6orf132,C6orf141,C6orf147,C6orf163,C6orf52,C6orf58,C6orf62,C6orf89,C7,C7orf25,C7orf33,C7orf57,C8A,C8B,C8G,C8orf33,C8orf34,C8orf44,C8orf48,C8orf58,C8orf74,C8orf76,C8orf82,C9,C9orf152,C9orf153,C9orf163,C9orf40,C9orf43,C9orf50,C9orf57,C9orf72,C9orf78,C9orf85,CA1,CA10,CA11,CA12,CA13,CA14,CA2,CA3,CA4,CA5A,CA5B,CA5BP1,CA6,CA7,CA8,CA9,CAAP1,CAB39,CAB39L,CABCOCO1,CABIN1,CABLES1,CABLES2,CABP1,CABP2,CABP4,CABP5,CABP7,CABS1,CABYR,CACFD1,CACHD1,CACNA1A,CACNA1B,CACNA1C,CACNA1D,CACNA1E,CACNA1F,CACNA1G,CACNA1H,CACNA1I,CACNA1S,CACNA2D1,CACNA2D2,CACNA2D3,CACNA2D4,CACNB1,CACNB2,CACNB3,CACNB4,CACNG1,CACNG2,CACNG3,CACNG4,CACNG5,CACNG6,CACNG7,CACNG8,CACTIN,CACUL1,CACYBP,CAD,CADM1,CADM2,CADM3,CADM4,CADPS,CADPS2,CAGE1,CALB1,CALB2,CALCA,CALCB,CALCOCO1,CALCOCO2,CALCR,CALCRL,CALD1,CALHM1,CALHM2,CALHM3,CALHM4,CALHM5,CALHM6,CALM1,CALM2,CALM3,CALML3,CALML4,CALML5,CALML6,CALN1,CALR,CALR3,CALU,CALY,CAMK1,CAMK1D,CAMK1G,CAMK2A,CAMK2B,CAMK2D,CAMK2G,CAMK2N1,CAMK2N2,CAMK4,CAMKK1,CAMKK2,CAMKMT,CAMKV,CAMLG,CAMP,CAMSAP1,CAMSAP2,CAMSAP3,CAMTA1,CAMTA2,CAND1,CAND2,CANT1,CANX,CAP1,CAP2,CAPG,CAPN1,CAPN10,CAPN11,CAPN12,CAPN13,CAPN14,CAPN15,CAPN2,CAPN3,CAPN5,CAPN6,CAPN7,CAPN8,CAPN9,CAPNS1,CAPNS2,CAPRIN1,CAPRIN2,CAPS,CAPS2,CAPSL,CAPZA1,CAPZA2,CAPZA3,CAPZB,CARD10,CARD11,CARD14,CARD16,CARD17P,CARD18,CARD19,CARD6,CARD8,CARD9,CARF,CARHSP1,CARINH,CARM1,CARMIL1,CARMIL2,CARMIL3,CARNMT1,CARNS1,CARS1,CARS2,CARTPT,CASC2,CASC3,CASD1,CASK,CASKIN1,CASKIN2,CASP1,CASP10,CASP12,CASP14,CASP2,CASP3,CASP4,CASP5,CASP6,CASP7,CASP8,CASP8AP2,CASP9,CASQ1,CASQ2,CASR,CASS4,CAST,CASTOR1,CASTOR3P,CASZ1,CAT,CATIP,CATSPER1,CATSPER2,CATSPER2P1,CATSPER3,CATSPER4,CATSPERB,CATSPERD,CATSPERE,CATSPERG,CATSPERZ,CAV1,CAV2,CAV3,CAVIN1,CAVIN2,CAVIN3,CAVIN4,CBARP,CBFA2T2,CBFA2T3,CBFB,CBL,CBLB,CBLC,CBLL1,CBLL2,CBLN1,CBLN2,CBLN3,CBLN4,CBR1,CBR3,CBR4,CBS,CBX1,CBX2,CBX3,CBX4,CBX5,CBX6,CBX7,CBX8,CBY1,CBY2,CBY3,CC2D1A,CC2D1B,CC2D2A,CC2D2B,CCAR1,C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FAM98C,FAM99B,FAM9A,FAM9B,FAM9C,FAN1,FANCA,FANCB,FANCC,FANCD2,FANCD2OS,FANCE,FANCF,FANCG,FANCI,FANCL,FANCM,FANK1,FAP,FAR1,FAR2,FARP1,FARP2,FARS2,FARSA,FARSB,FAS,FAS-AS1,FASLG,FASN,FASTK,FASTKD1,FASTKD2,FASTKD3,FASTKD5,FAT1,FAT2,FAT3,FAT4,FATE1,FAU,FAXC,FAXDC2,FBF1,FBH1,FBL,FBLIM1,FBLL1,FBLN1,FBLN2,FBLN5,FBLN7,FBN1,FBN2,FBN3,FBP1,FBP2,FBRS,FBRSL1,FBXL12,FBXL13,FBXL14,FBXL15,FBXL16,FBXL17,FBXL18,FBXL19,FBXL19-AS1,FBXL2,FBXL20,FBXL21P,FBXL22,FBXL3,FBXL4,FBXL5,FBXL6,FBXL7,FBXL8,FBXL9P,FBXO10,FBXO11,FBXO15,FBXO16,FBXO17,FBXO2,FBXO21,FBXO22,FBXO24,FBXO25,FBXO27,FBXO28,FBXO3,FBXO30,FBXO31,FBXO32,FBXO33,FBXO34,FBXO36,FBXO38,FBXO39,FBXO4,FBXO40,FBXO41,FBXO42,FBXO43,FBXO44,FBXO45,FBXO46,FBXO47,FBXO48,FBXO5,FBXO6,FBXO7,FBXO8,FBXO9,FBXW10,FBXW10B,FBXW11,FBXW12,FBXW2,FBXW4,FBXW4P1,FBXW5,FBXW7,FBXW8,FBXW9,FCAMR,FCAR,FCER1A,FCER1G,FCER2,FCF1,FCGBP,FCGR1A,FCGR2A,FCGR2B,FCGR3A,FCGRT,FCHO1,FCHO2,FCHSD1,FCHSD2,FCMR,FCN1,FCN2,FCN3,FCRL1,FCRL2,FCRL3,FCRL4,FCRL5,FCRL6,FCRLA,FCRLB,FCSK,FDCSP,FDFT1,FDPS,FDPSP2,FDX1,FDX2,FDXACB1,FDXR,FECH,FEM1A,FEM1B,FEM1C,FEN1,FER,FER1L4,FER1L5,FER1L6,FERD3L,FERMT1,FERMT2,FERMT3,FERRY3,FES,FETUB,FEV,FEZ1,FEZ2,FEZF1,FEZF2,FFAR1,FFAR2,FFAR3,FFAR4,FGA,FGB,FGD1,FGD2,FGD3,FGD4,FGD5,FGD6,FGF1,FGF10,FGF11,FGF12,FGF13,FGF14,FGF16,FGF17,FGF18,FGF19,FGF2,FGF20,FGF21,FGF22,FGF23,FGF3,FGF4,FGF5,FGF6,FGF7,FGF8,FGF9,FGFBP1,FGFBP2,FGFBP3,FGFR1,FGFR1OP2,FGFR2,FGFR3,FGFR4,FGFRL1,FGG,FGGY,FGL1,FGL2,FGR,FH,FHAD1,FHDC1,FHIP1A,FHIP1B,FHIP2A,FHIP2B,FHIT,FHL1,FHL2,FHL3,FHL5,FHOD1,FHOD3,FIBCD1,FIBIN,FIBP,FICD,FIG4,FIGLA,FIGN,FIGNL1,FIGNL2,FILIP1,FILIP1L,FIP1L1,FIRRM,FIS1,FITM1,FITM2,FIZ1,FJX1,FKBP10,FKBP11,FKBP14,FKBP15,FKBP1A,FKBP1AP1,FKBP1B,FKBP2,FKBP3,FKBP4,FKBP5,FKBP6,FKBP7,FKBP8,FKBP9,FKBP9P1,FKRP,FKSG29,FKTN,FLACC1,FLAD1,FLCN,FLG,FLG2,FLI1,FLII,FLJ12825,FLJ13224,FLJ16779,FLJ30679,FLJ36000,FLJ37201,FLJ40194,FLJ42393,FLNA,FLNB,FLNC,FLOT2,FLRT1,FLRT2,FLRT3,FLT1,FLT3,FLT3LG,FLT4,FLVCR1,FLVCR1-DT,FLVCR2,FLYWCH1,FLYWCH2,FMC1-LUC7L2,FMN1,FMN2,FMNL1,FMNL2,FMNL3,FMO1,FMO2,FMO3,FMO4,FMO5,FMO6P,FMO9P,FMOD,FMR1,FMR1-AS1,FMR1NB,FN1,FN3K,FN3KRP,FNBP1,FNBP1L,FNBP4,FNDC1,FNDC11,FNDC3A,FNDC3B,FNDC4,FNDC5,FNDC7,FNDC8,FNDC9,FNIP1,FNIP2,FNTA,FNTB,FOCAD,FOLH1,FOLH1B,FOLR1,FOLR2,FOLR3,FOS,FOSB,FOSL1,FOSL2,FOXA1,FOXA2,FOXA3,FOXB1,FOXB2,FOXC1,FOXC2,FOXD1,FOXD2,FOXD3,FOXD4,FOXD4L1,FOXE1,FOXE3,FOXF1,FOXF2,FOXG1,FOXH1,FOXI1,FOXI2,FOXI3,FOXJ1,FOXJ2,FOXJ3,FOXK1,FOXK2,FOXL1,FOXL2,FOXL2NB,FOXM1,FOXN1,FOXN2,FOXN3,FOXN3-AS2,FOXN4,FOXO1,FOXO3,FOXO3B,FOXO4,FOXP1,FOXP2,FOXP3,FOXP4,FOXQ1,FOXR1,FOXR2,FOXRED1,FOXRED2,FOXS1,FPGS,FPGT,FPR1,FPR2,FPR3,FRA10AC1,FRAS1,FRAT1,FRAT2,FREM1,FREM2,FREM3,FREY1,FRG1,FRG1BP,FRG2B,FRK,FRMD1,FRMD3,FRMD4A,FRMD4B,FRMD5,FRMD6,FRMD7,FRMD8,FRMPD1,FRMPD2,FRMPD2B,FRMPD4,FRRS1,FRRS1L,FRS2,FRS3,FRY,FRYL,FRZB,FSAF1,FSCB,FSCN1,FSCN2,FSCN3,FSD1,FSD1L,FSD2,FSHB,FSHR,FSIP1,FSIP2,FST,FSTL1,FSTL3,FSTL4,FSTL5,FTCD,FTH1,FTH1P3,FTHL17,FTL,FTLP10,FTMT,FTO,FTSJ1,FTSJ3,FTX,FUBP1,FUBP3,FUCA1,FUCA2,FUNDC1,FUNDC2,FUNDC2P2,FUOM,FURIN,FUS,FUT1,FUT10,FUT11,FUT2,FUT3,FUT4,FUT5,FUT6,FUT7,FUT8,FUT9,FUZ,FXN,FXR1,FXR2,FXYD1,FXYD2,FXYD3,FXYD4,FXYD5,FXYD6,FXYD7,FYB1,FYB2,FYCO1,FYN,FYTTD1,FZD1,FZD10,FZD2,FZD3,FZD4,FZD5,FZD6,FZD7,FZD8,FZD9,FZR1,G0S2,G2E3,G3BP1,G3BP2,G6PC1,G6PC2,G6PC3,G6PD,GAA,GAB1,GAB2,GAB3,GAB4,GABARAP,GABARAPL1,GABARAPL2,GABARAPL3,GABBR2,GABPA,GABPB1,GABPB2,GABRA1,GABRA2,GABRA3,GABRA4,GABRA5,GABRA6,GABRB1,GABRB2,GABRB3,GABRD,GABRE,GABRG1,GABRG2,GABRG3,GABRP,GABRQ,GABRR1,GABRR2,GABRR3,GAD1,GAD2,GADD45A,GADD45B,GADD45G,GADD45GIP1,GADL1,GAGE1,GAGE10,GAK,GAL,GAL3ST1,GAL3ST2,GAL3ST3,GAL3ST4,GALC,GALE,GALK1,GALK2,GALM,GALNS,GALNT1,GALNT10,GALNT11,GALNT12,GALNT13,GALNT14,GALNT15,GALNT16,GALNT17,GALNT18,GALNT2,GALNT3,GALNT4,GALNT5,GALNT6,GALNT7,GALNT8,GALNT9,GALNTL5,GALNTL6,GALP,GALR1,GALR2,GALR3,GALT,GAMT,GAN,GANAB,GANC,GAP43,GAPDH,GAPDHS,GAPT,GAPVD1,GAR1,GAREM1,GAREM2,GARIN1A,GARIN1B,GARIN2,GARIN3,GARIN4,GARIN5A,GARIN5B,GARIN6,GARNL3,GARRE1,GART,GAS1,GAS2,GAS2L1,GAS2L2,GAS2L3,GAS5,GAS6,GAS7,GAS8,GAS8-AS1,GASK1A,GASK1B,GAST,GATA1,GATA2,GATA3,GATA4,GATA5,GATA6,GATAD1,GATAD2A,GATAD2B,GATB,GATC,GATD1,GATD3,GATM,GBA1LP,GBA2,GBA3,GBE1,GBF1,GBGT1,GBP1,GBP1P1,GBP2,GBP3,GBP4,GBP5,GBP6,GBP7,GBX1,GBX2,GC,GCA,GCAT,GCC1,GCC2,GCDH,GCFC2,GCG,GCGR,GCH1,GCHFR,GCK,GCKR,GCLC,GCLM,GCM1,GCM2,GCN1,GCNA,GCNT1,GCNT2,GCNT3,GCNT4,GCNT7,GCOM1,GCSAM,GCSAML,GCSH,GDA,GDAP1,GDAP1L1,GDAP2,GDE1,GDF1,GDF10,GDF11,GDF15,GDF2,GDF3,GDF5,GDF6,GDF7,GDF9,GDI1,GDI2,GDNF,GDPD1,GDPD2,GDPD3,GDPD4,GDPD5,GDPGP1,GEM,GEMIN4,GEMIN5,GEMIN6,GEMIN7,GEMIN8,GEMIN8P4,GEN1,GET1,GET3,GET4,GFAP,GFER,GFI1,GFI1B,GFM1,GFM2,GFOD1,GFOD2,GFOD3P,GFPT1,GFPT2,GFRA1,GFRA2,GFRA3,GFRA4,GFRAL,GFUS,GGA1,GGA2,GGA3,GGACT,GGCT,GGCX,GGH,GGN,GGNBP1,GGNBP2,GGPS1,GGT1,GGT3P,GGT5,GGT6,GGT7,GGT8P,GGTA1,GGTLC1,GGTLC2,GH1,GH2,GHDC,GHITM,GHR,GHRH,GHRHR,GHRL,GHRLOS,GHSR,GID4,GID8,GIGYF1,GIGYF2,GIMAP1,GIMAP2,GIMAP4,GIMAP5,GIMAP6,GIMAP7,GIMAP8,GIN1,GINM1,GINS1,GINS2,GINS3,GINS4,GIP,GIPC1,GIPC2,GIPC3,GIPR,GIT1,GIT2,GJA1,GJA10,GJA3,GJA4,GJA5,GJA8,GJA9,GJA9-MYCBP,GJB1,GJB2,GJB3,GJB4,GJB5,GJB6,GJB7,GJC1,GJC2,GJC3,GJD2,GJD3,GJD4,GK,GK2,GK3,GK5,GKAP1,GKN1,GKN2,GLA,GLB1,GLB1L,GLB1L2,GLB1L3,GLCCI1,GLCE,GLDC,GLDN,GLE1,GLG1,GLI1,GLI2,GLI3,GLI4,GLIPR1,GLIPR1L1,GLIPR1L2,GLIPR2,GLIS1,GLIS2,GLIS3,GLIS3-AS1,GLMN,GLMP,GLO1,GLOD4,GLOD5,GLP1R,GLP2R,GLRA1,GLRA2,GLRA3,GLRA4,GLRB,GLRX,GLRX2,GLRX3,GLRX5,GLS,GLS2,GLT1D1,GLT6D1,GLT8D1,GLT8D2,GLTP,GLTPD2,GLUD1,GLUD2,GLUL,GLYAT,GLYATL1,GLYATL2,GLYATL3,GLYCAM1,GLYCTK,GLYR1,GM2A,GMCL1,GMCL2,GMDS,GMEB1,GMEB2,GMFB,GMFG,GMIP,GML,GMNC,GMNN,GMPPA,GMPPB,GMPR,GMPR2,GMPS,GNA11,GNA12,GNA13,GNA14,GNA15,GNAI1,GNAI2,GNAI3,GNAL,GNAO1,GNAQ,GNAS,GNAS-AS1,GNAT1,GNAT2,GNAT3,GNAZ,GNB1,GNB1L,GNB2,GNB3,GNB4,GNB5,GNE,GNG10,GNG11,GNG12,GNG13,GNG2,GNG3,GNG4,GNG5,GNG7,GNG8,GNGT1,GNGT2,GNL2,GNL3,GNL3L,GNLY,GNMT,GNPAT,GNPDA1,GNPDA2,GNPNAT1,GNPTAB,GNPTG,GNRH1,GNRH2,GNRHR,GNRHR2,GNS,GOLGA1,GOLGA2,GOLGA2P5,GOLGA3,GOLGA4,GOLGA5,GOLGA6A,GOLGA6B,GOLGA6C,GOLGA6D,GOLGA6L1,GOLGA6L10,GOLGA6L5P,GOLGA6L6,GOLGA7,GOLGA7B,GOLGA8A,GOLGA8B,GOLGA8CP,GOLGA8DP,GOLGA8EP,GOLGA8IP,GOLGB1,GOLIM4,GOLM1,GOLM2,GOLPH3,GOLPH3L,GOLT1A,GOLT1B,GON4L,GON7,GOPC,GORAB,GORASP1,GORASP2,GOSR1,GOSR2,GOT1,GOT1L1,GOT2,GP1BA,GP1BB,GP2,GP5,GP6,GP9,GPA33,GPAA1,GPALPP1,GPAM,GPAT2,GPAT3,GPAT4,GPATCH1,GPATCH11,GPATCH2,GPATCH2L,GPATCH3,GPATCH4,GPATCH8,GPBAR1,GPBP1,GPBP1L1,GPC1,GPC2,GPC3,GPC4,GPC5,GPC6,GPCPD1,GPD1,GPD1L,GPD2,GPER1,GPHA2,GPHB5,GPHN,GPI,GPIHBP1,GPKOW,GPLD1,GPM6A,GPM6B,GPN1,GPN2,GPN3,GPNMB,GPR101,GPR107,GPR108,GPR119,GPR12,GPR132,GPR135,GPR137,GPR137B,GPR137C,GPR139,GPR141,GPR142,GPR143,GPR146,GPR148,GPR149,GPR15,GPR150,GPR151,GPR152,GPR153,GPR155,GPR156,GPR157,GPR158,GPR15LG,GPR160,GPR161,GPR162,GPR17,GPR171,GPR173,GPR174,GPR176,GPR179,GPR18,GPR180,GPR182,GPR183,GPR19,GPR20,GPR21,GPR22,GPR25,GPR26,GPR27,GPR3,GPR31,GPR32,GPR33,GPR34,GPR35,GPR37,GPR37L1,GPR39,GPR4,GPR45,GPR50,GPR52,GPR55,GPR6,GPR61,GPR62,GPR63,GPR65,GPR68,GPR75,GPR75-ASB3,GPR78,GPR82,GPR83,GPR84,GPR85,GPR87,GPR88,GPR89B,GPRASP1,GPRASP2,GPRC5A,GPRC5B,GPRC5C,GPRC5D,GPRC6A,GPRIN1,GPRIN2,GPRIN3,GPS1,GPS2,GPSM1,GPSM2,GPT,GPT2,GPX1,GPX2,GPX3,GPX4,GPX6,GPX7,GPX8,GRAMD1A,GRAMD1B,GRAMD1C,GRAMD2A,GRAMD2B,GRAMD4,GRAP,GRAP2,GRB10,GRB14,GRB2,GRB7,GREB1,GREB1L,GREM1,GREM2,GRHL1,GRHL2,GRHL3,GRHPR,GRIA1,GRIA2,GRIA3,GRIA4,GRID1,GRID2,GRID2IP,GRIK1,GRIK1-AS1,GRIK1-AS2,GRIK2,GRIK3,GRIK4,GRIK5,GRIN1,GRIN2A,GRIN2B,GRIN2C,GRIN2D,GRIN3A,GRIN3B,GRINA,GRIP1,GRIP2,GRIPAP1,GRK1,GRK2,GRK3,GRK4,GRK5,GRK6,GRK7,GRM1,GRM2,GRM3,GRM4,GRM5,GRM6,GRM7,GRM8,GRN,GRP,GRPEL1,GRPEL2,GRPR,GRSF1,GRTP1,GRWD1,GRXCR1,GRXCR2,GSAP,GSC,GSC2,GSDMA,GSDMB,GSDMC,GSDMD,GSDME,GSE1,GSG1,GSG1L,GSK3A,GSK3B,GSKIP,GSN,GSPT1,GSPT2,GSR,GSS,GSTA1,GSTA2,GSTA3,GSTA4,GSTA5,GSTA7P,GSTCD,GSTK1,GSTM1,GSTM2,GSTM2P1,GSTM3,GSTM4,GSTM5,GSTO1,GSTO2,GSTP1,GSTT1,GSTT4,GSTTP2,GSTZ1,GSX1,GSX2,GTDC1,GTF2A1,GTF2A1L,GTF2A2,GTF2B,GTF2E1,GTF2E2,GTF2F1,GTF2F2,GTF2H1,GTF2H2C,GTF2H2C_2,GTF2H3,GTF2H5,GTF2I,GTF2IP1,GTF2IRD1,GTF2IRD2,GTF2IRD2B,GTF2IRD2P1,GTF3A,GTF3C1,GTF3C2,GTF3C3,GTF3C4,GTF3C5,GTF3C6,GTPBP1,GTPBP10,GTPBP2,GTPBP3,GTPBP4,GTPBP8,GTSE1,GTSF1,GTSF1L,GUCA1A,GUCA1B,GUCA1C,GUCA2A,GUCA2B,GUCD1,GUCY1A1,GUCY1A2,GUCY1B1,GUCY1B2,GUCY2C,GUCY2D,GUCY2EP,GUCY2F,GUCY2GP,GUF1,GUK1,GULP1,GUSB,GUSBP1,GUSBP2,GUSBP4,GVINP1,GXYLT1,GXYLT2,GYG1,GYG2,GYG2P1,GYPA,GYPB,GYPC,GYPE,GYS1,GYS2,GZF1,GZMA,GZMB,GZMH,GZMK,GZMM,H1-0,H1-1,H1-10,H1-10-AS1,H1-2,H1-3,H1-4,H1-5,H1-6,H1-7,H1-8,H1-9P,H19,H2AC1,H2AC11,H2AC12,H2AC14,H2AC15,H2AC16,H2AC17,H2AC20,H2AC21,H2AC25,H2AC4,H2AC6,H2AC7,H2AC8,H2AJ,H2AX,H2AZ1,H2AZ2,H2BC1,H2BC10,H2BC11,H2BC12,H2BC13,H2BC14,H2BC15,H2BC17,H2BC18,H2BC20P,H2BC21,H2BC26,H2BC3,H2BC4,H2BC5,H2BC6,H2BC7,H2BC8,H2BC9,H2BW1,H2BW2,H2BW4P,H3-3A,H3-3B,H3-4,H3-5,H3C1,H3C10,H3C11,H3C12,H3C2,H3C3,H3C4,H3C6,H3C7,H3C8,H4C1,H4C11,H4C12,H4C13,H4C16,H4C2,H4C3,H4C4,H4C5,H4C6,H4C7,H4C8,H4C9,H6PD,HAAO,HABP2,HABP4,HACD1,HACD2,HACD3,HACD4,HACE1,HACL1,HADH,HADHA,HADHB,HAGH,HAGHL,HAL,HAMP,HAND1,HAND2,HAND2-AS1,HAO1,HAO2,HAP1,HAPLN1,HAPLN2,HAPLN3,HAPLN4,HAPSTR1,HAR1A,HAR1B,HARBI1,HARS1,HARS2,HAS1,HAS2,HAS2-AS1,HAS3,HASPIN,HAT1,HAUS1,HAUS2,HAUS3,HAUS4,HAUS5,HAUS6,HAUS7,HAUS8,HAVCR1,HAVCR2,HAX1,HBB,HBBP1,HBD,HBE1,HBEGF,HBM,HBP1,HBQ1,HBS1L,HBZ,HCAR1,HCAR2,HCAR3,HCCAT5,HCCS,HCFC1,HCFC1R1,HCFC2,HCG11,HCK,HCLS1,HCN1,HCN2,HCN3,HCN4,HCRT,HCRTR1,HCRTR2,HCST,HDAC1,HDAC10,HDAC11,HDAC2,HDAC3,HDAC4,HDAC5,HDAC6,HDAC7,HDAC8,HDAC9,HDC,HDDC2,HDDC3,HDGF,HDGFL1,HDGFL2,HDGFL3,HDHD2,HDHD3,HDHD5,HDHD5-AS1,HDLBP,HDX,HEATR1,HEATR3,HEATR4,HEATR5A,HEATR5B,HEATR6,HEATR9,HEBP1,HEBP2,HECA,HECTD1,HECTD2,HECTD3,HECTD4,HECW1,HECW2,HEG1,HELB,HELLS,HELQ,HELT,HELZ,HELZ2,HEMGN,HEMK1,HENMT1,HEPACAM,HEPACAM2,HEPH,HEPHL1,HEPN1,HERC1,HERC2,HERC2P2,HERC2P3,HERC2P9,HERC3,HERC4,HERC5,HERC6,HERPUD1,HERPUD2,HES1,HES2,HES3,HES4,HES5,HES6,HES7,HESX1,HEXA,HEXA-AS1,HEXB,HEXD,HEXIM1,HEXIM2,HEY1,HEY2,HEYL,HFE,HFM1,HGD,HGF,HGFAC,HGS,HGSNAT,HHAT,HHATL,HHEX,HHIP,HHIPL1,HHIPL2,HHLA1,HHLA2,HIBADH,HIBCH,HIC1,HIC2,HID1,HIF1A,HIF1AN,HIF3A,HIGD1A,HIGD1B,HIGD1C,HIGD2A,HIGD2B,HIKESHI,HILPDA,HINFP,HINT1,HINT2,HINT3,HIP1,HIP1R,HIPK1,HIPK2,HIPK3,HIPK4,HIRA,HIRIP3,HIVEP1,HIVEP2,HIVEP3,HJURP,HJV,HK1,HK2,HK3,HKDC1,HLA-A,HLA-B,HLA-C,HLA-DRA,HLA-DRB1,HLA-DRB3,HLA-DRB5,HLA-DRB6,HLA-G,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TK,MATN1,MATN2,MATN3,MATN4,MATR3,MAU2,MAVS,MAX,MAZ,MB,MB21D2,MBD1,MBD2,MBD3,MBD3L1,MBD4,MBD5,MBD6,MBIP,MBL1P,MBL2,MBLAC1,MBLAC2,MBNL1,MBNL2,MBNL3,MBOAT1,MBOAT2,MBOAT4,MBOAT7,MBP,MBTD1,MBTPS1,MBTPS2,MC1R,MC2R,MC3R,MC4R,MC5R,MCAM,MCAT,MCC,MCCC1,MCCC2,MCEE,MCEMP1,MCF2,MCF2L,MCF2L2,MCFD2,MCHR1,MCHR2,MCL1,MCM10,MCM2,MCM3,MCM3AP,MCM3AP-AS1,MCM4,MCM5,MCM6,MCM7,MCM8,MCM9,MCMBP,MCMDC2,MCOLN1,MCOLN2,MCOLN3,MCPH1,MCRIP1,MCRIP2,MCRS1,MCTP1,MCTP2,MCTS1,MCTS2,MCU,MCUB,MCUR1,MDFI,MDFIC,MDGA1,MDGA2,MDH1,MDH1B,MDH2,MDK,MDM1,MDM2,MDM4,MDN1,MDP1,MDS2,ME1,ME2,ME3,MEA1,MEAF6,MEAK7,MECOM,MECP2,MECR,MED1,MED10,MED11,MED12,MED12L,MED13,MED13L,MED14,MED15,MED16,MED17,MED18,MED19,MED20,MED21,MED22,MED23,MED24,MED25,MED26,MED27,MED28,MED29,MED30,MED31,MED4,MED6,MED7,MED8,MED9,MEDAG,MEF2A,MEF2B,MEF2C,MEF2D,MEFV,MEG3,MEG8,MEGF10,MEGF11,MEGF6,MEGF8,MEGF9,MEI1,MEIG1,MEIOB,MEIOC,MEIS1,MEIS2,MEIS3,MEIS3P1,MELK,MELTF,MEMO1,MEN1,MEOX1,MEOX2,MEP1A,MEP1B,MEPCE,MEPE,MERTK,MESD,MESP1,MESP2,MEST,MESTIT1,MET,METAP1,METAP1D,METAP2,METRN,METRNL,METTL1,METTL13,METTL14,METTL15,METTL16,METTL17,METTL18,METTL21A,METTL21C,METTL22,METTL23,METTL24,METTL25,METTL25B,METTL26,METTL27,METTL2A,METTL2B,METTL3,METTL4,METTL5,METTL6,METTL8,METTL9,MEX3A,MEX3B,MEX3C,MEX3D,MFAP1,MFAP2,MFAP3,MFAP3L,MFAP4,MFAP5,MFF,MFGE8,MFHAS1,MFN1,MFN2,MFNG,MFRP,MFSD1,MFSD10,MFSD11,MFSD12,MFSD13A,MFSD14A,MFSD14B,MFSD2A,MFSD2B,MFSD3,MFSD4A,MFSD4B,MFSD5,MFSD6,MFSD6L,MFSD8,MFSD9,MGA,MGAM,MGARP,MGAT1,MGAT2,MGAT3,MGAT4A,MGAT4B,MGAT4C,MGAT5,MGAT5B,MGC12916,MGC15885,MGC16025,MGC16275,MGC27382,MGC2889,MGLL,MGME1,MGMT,MGP,MGRN1,MGST1,MGST2,MGST3,MIA,MIA2,MIA3,MIAT,MIB1,MIB2,MICAL1,MICAL2,MICAL3,MICALL1,MICALL2,MICOS10,MICOS13,MICU1,MICU2,MICU3,MID1,MID1IP1,MID2,MIDEAS,MIDN,MIEF1,MIEF2,MIEN1,MIER1,MIER2,MIER3,MIF,MIF4GD,MIGA1,MIGA2,MIIP,MILR1,MIMT1,MINAR1,MINDY1,MINDY2,MINDY3,MINDY4,MINK1,MINPP1,MIOS,MIOX,MIP,MIPEP,MIPOL1,MIR1-1,MIR1-1HG,MIR1-2,MIR100,MIR100HG,MIR101-1,MIR101-2,MIR103A1,MIR103A2,MIR105-1,MIR105-2,MIR106A,MIR106B,MIR107,MIR10A,MIR10B,MIR1181,MIR1197,MIR1204,MIR122,MIR1225,MIR1227,MIR1234,MIR124-2,MIR124-3,MIR1247,MIR1250,MIR1251,MIR1257,MIR125A,MIR125B1,MIR125B2,MIR126,MIR127,MIR128-1,MIR128-2,MIR1281,MIR1282,MIR1284,MIR1287,MIR129-1,MIR129-2,MIR1292,MIR1298,MIR1307,MIR130A,MIR130B,MIR132,MIR133A1,MIR133A2,MIR133B,MIR134,MIR135A1,MIR135A2,MIR136,MIR137,MIR138-1,MIR138-2,MIR139,MIR140,MIR141,MIR142,MIR143,MIR144,MIR145,MIR146A,MIR147A,MIR147B,MIR148A,MIR148B,MIR149,MIR150,MIR152,MIR153-1,MIR153-2,MIR1538,MIR1539,MIR154,MIR155,MIR155HG,MIR15A,MIR15B,MIR16-1,MIR16-2,MIR17,MIR17HG,MIR181A1,MIR181A2,MIR181B1,MIR181B2,MIR181C,MIR182,MIR183,MIR184,MIR185,MIR186,MIR187,MIR188,MIR18A,MIR18B,MIR1909,MIR190A,MIR191,MIR1915HG,MIR192,MIR193A,MIR194-1,MIR194-2,MIR195,MIR196A1,MIR196A2,MIR197,MIR198,MIR199A1,MIR199A2,MIR199B,MIR19A,MIR19B1,MIR19B2,MIR200A,MIR200B,MIR200C,MIR202,MIR203A,MIR204,MIR205,MIR206,MIR208A,MIR20A,MIR21,MIR210,MIR211,MIR2110,MIR212,MIR214,MIR215,MIR216A,MIR216B,MIR217,MIR218-1,MIR218-2,MIR219A2,MIR22,MIR221,MIR222,MIR223,MIR22HG,MIR23A,MIR23B,MIR24-1,MIR24-2,MIR25,MIR26A1,MIR26A2,MIR26B,MIR27A,MIR27B,MIR2861,MIR296,MIR299,MIR29A,MIR29B1,MIR29B2,MIR29C,MIR301A,MIR302A,MIR302B,MIR3074,MIR30A,MIR30B,MIR30C1,MIR30C2,MIR30D,MIR30E,MIR31,MIR3120,MIR3128,MIR3130-2,MIR3150B,MIR3167,MIR3188,MIR3189,MIR32,MIR320A,MIR320C1,MIR323A,MIR323B,MIR326,MIR330,MIR331,MIR339,MIR33A,MIR34A,MIR34B,MIR34C,MIR3605,MIR3610,MIR3615,MIR3618,MIR3620,MIR3621,MIR3652,MIR3655,MIR365A,MIR3661,MIR3665,MIR3667HG,MIR3684,MIR3714,MIR374B,MIR374C,MIR375,MIR376C,MIR377,MIR378C,MIR382,MIR3917,MIR3936,MIR3939,MIR3940,MIR3943,MIR3944,MIR410,MIR412,MIR423,MIR425,MIR4260,MIR4263,MIR4287,MIR4289,MIR429,MIR4292,MIR4294,MIR4321,MIR4325,MIR451A,MIR455,MIR485,MIR487A,MIR487B,MIR492,MIR494,MIR495,MIR503,MIR504,MIR516B1,MIR516B2,MIR543,MIR551B,MIR555,MIR564,MIR567,MIR573,MIR589,MIR598,MIR600HG,MIR601,MIR604,MIR621,MIR622,MIR626,MIR628,MIR630,MIR632,MIR635,MIR636,MIR637,MIR639,MIR646HG,MIR658,MIR659,MIR663B,MIR664A,MIR670,MIR671,MIR675,MIR7-1,MIR7-2,MIR7-3,MIR7-3HG,MIR711,MIR718,MIR744,MIR765,MIR766,MIR770,MIR873,MIR874,MIR890,MIR892A,MIR892B,MIR9-1,MIR9-1HG,MIR9-2,MIR9-3,MIR92A1,MIR92A2,MIR93,MIR935,MIR936,MIR939,MIR940,MIR943,MIR96,MIR98,MIR99A,MIR99AHG,MIR99B,MIRLET7A1,MIRLET7A2,MIRLET7A3,MIRLET7B,MIRLET7C,MIRLET7D,MIRLET7E,MIRLET7F1,MIRLET7F2,MIRLET7G,MIRLET7I,MIS12,MIS18A,MIS18BP1,MISP,MITD1,MITF,MIX23,MIXL1,MKI67,MKKS,MKLN1,MKNK1,MKNK2,MKRN1,MKRN2,MKRN3,MKRN7P,MKRN9P,MKS1,MKX,MLANA,MLC1,MLEC,MLF1,MLF2,MLH1,MLH3,MLIP,MLKL,MLLT1,MLLT10,MLLT11,MLLT3,MLLT6,MLN,MLNR,MLPH,MLST8,MLX,MLXIP,MLXIPL,MLYCD,MMAA,MMAB,MMACHC,MMADHC,MMD,MMD2,MME,MMEL1,MMGT1,MMP1,MMP10,MMP11,MMP12,MMP13,MMP14,MMP15,MMP16,MMP17,MMP19,MMP2,MMP20,MMP21,MMP24,MMP25,MMP26,MMP27,MMP28,MMP3,MMP7,MMP8,MMP9,MMRN1,MMRN2,MMS19,MMS22L,MMUT,MN1,MNAT1,MND1,MNDA,MNS1,MNT,MNX1,MOAP1,MOB1A,MOB1B,MOB2,MOB3A,MOB3B,MOB3C,MOB4,MOBP,MOCOS,MOCS1,MOCS2,MOCS3,MOGAT1,MOGAT2,MOGAT3,MOGS,MOK,MON1A,MON1B,MON2,MORC1,MORC2,MORC2-AS1,MORC3,MORC4,MORF4,MORF4L1,MORF4L2,MORN1,MORN2,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,NIPAL3,NIPAL4,NIPBL,NIPSNAP1,NIPSNAP2,NIPSNAP3A,NIPSNAP3B,NISCH,NIT1,NIT2,NKAIN1,NKAIN2,NKAIN3,NKAIN4,NKAP,NKAPD1,NKAPL,NKAPP1,NKD1,NKD2,NKG7,NKIRAS1,NKIRAS2,NKPD1,NKRF,NKTR,NKX1-2,NKX2-1,NKX2-2,NKX2-3,NKX2-4,NKX2-5,NKX2-6,NKX2-8,NKX3-1,NKX3-2,NKX6-1,NKX6-2,NKX6-3,NLE1,NLGN1,NLGN2,NLGN3,NLGN4X,NLGN4Y,NLK,NLN,NLRC3,NLRC4,NLRC5,NLRP1,NLRP10,NLRP11,NLRP12,NLRP13,NLRP14,NLRP2,NLRP3,NLRP4,NLRP5,NLRP6,NLRP7,NLRP8,NLRP9,NLRX1,NMB,NMBR,NMD3,NME1,NME1-NME2,NME2,NME3,NME4,NME5,NME6,NME7,NME8,NME9,NMI,NMNAT1,NMNAT2,NMNAT3,NMRAL1,NMRK1,NMRK2,NMS,NMT1,NMT2,NMU,NMUR1,NMUR2,NNAT,NNMT,NNT,NOA1,NOB1,NOBOX,NOC2L,NOC3L,NOC4L,NOCT,NOD1,NOD2,NODAL,NOG,NOL10,NOL11,NOL12,NOL3,NOL4,NOL4L,NOL6,NOL7,NOL8,NOL9,NOLC1,NOM1,NONO,NOP10,NOP14,NOP14-AS1,NOP16,NOP2,NOP53,NOP56,NOP58,NOP9,NOPCHAP1,NOS1,NOS1AP,NOS2,NOS3,NOSIP,NOSTRIN,NOTCH1,NOTCH2,NOTCH2NLB,NOTCH3,NOTO,NOTUM,NOVA1,NOVA2,NOX1,NOX3,NOX4,NOX5,NOXA1,NOXO1,NOXRED1,NPAP1,NPAS1,NPAS2,NPAS3,NPAS4,NPAT,NPB,NPBWR1,NPBWR2,NPC1,NPC1L1,NPC2,NPDC1,NPEPL1,NPEPPS,NPFF,NPFFR1,NPFFR2,NPHP1,NPHP3,NPHP3-AS1,NPHP4,NPHS1,NPHS2,NPIPB3,NPL,NPLOC4,NPM1,NPM2,NPM3,NPNT,NPPA,NPPB,NPPC,NPR1,NPR2,NPR3,NPRL2,NPRL3,NPS,NPSR1,NPTN,NPTX1,NPTX2,NPTXR,NPVF,NPW,NPY,NPY1R,NPY2R,NPY4R2,NPY5R,NPY6R,NQO1,NQO2,NR0B1,NR0B2,NR1D1,NR1D2,NR1H2,NR1H3,NR1H4,NR1I2,NR1I3,NR2C1,NR2C2,NR2C2AP,NR2E1,NR2E3,NR2F1,NR2F2,NR2F6,NR3C1,NR3C2,NR4A1,NR4A2,NR4A3,NR5A1,NR5A2,NR6A1,NRAD1,NRADDP,NRAP,NRARP,NRAS,NRBF2,NRBP1,NRBP2,NRCAM,NRDC,NRDE2,NREP,NRF1,NRG1,NRG2,NRG3,NRG4,NRGN,NRIP1,NRIP2,NRIP3,NRK,NRL,NRN1,NRN1L,NRP1,NRP2,NRROS,NRSN1,NRSN2,NRTN,NRXN1,NRXN2,NRXN3,NSA2,NSD1,NSD2,NSD3,NSDHL,NSF,NSFL1C,NSG1,NSG2,NSL1,NSMAF,NSMCE1,NSMCE2,NSMCE3,NSMCE4A,NSMF,NSRP1,NSUN2,NSUN3,NSUN4,NSUN5,NSUN5P1,NSUN5P2,NSUN6,NSUN7,NT5C,NT5C1A,NT5C1B,NT5C2,NT5C3A,NT5C3B,NT5DC1,NT5DC2,NT5DC3,NT5E,NT5M,NTAN1,NTAQ1,NTF3,NTF4,NTHL1,NTM,NTMT1,NTMT2,NTN1,NTN3,NTN4,NTN5,NTNG1,NTNG2,NTPCR,NTRK1,NTRK2,NTRK3,NTS,NTSR1,NTSR2,NUAK1,NUAK2,NUB1,NUBP1,NUBP2,NUBPL,NUCB1,NUCB2,NUCKS1,NUDC,NUDCD1,NUDCD2,NUDCD3,NUDT1,NUDT10,NUDT11,NUDT12,NUDT13,NUDT14,NUDT15,NUDT16,NUDT16L1,NUDT16L2P,NUDT17,NUDT18,NUDT19,NUDT2,NUDT21,NUDT22,NUDT3,NUDT4,NUDT5,NUDT6,NUDT7,NUDT8,NUDT9,NUDT9P1,NUF2,NUFIP1,NUFIP2,NUGGC,NUMA1,NUMB,NUMBL,NUP107,NUP133,NUP153,NUP155,NUP160,NUP188,NUP205,NUP210,NUP210L,NUP210P1,NUP214,NUP35,NUP37,NUP42,NUP43,NUP50,NUP54,NUP58,NUP62,NUP62CL,NUP85,NUP88,NUP93,NUP98,NUPR1,NUS1,NUSAP1,NUTF2,NUTM1,NUTM2A,NUTM2D,NUTM2F,NUTM2G,NVL,NWD1,NWD2,NXF1,NXF2,NXF2B,NXF3,NXF4,NXF5,NXN,NXNL1,NXNL2,NXPE1,NXPE2,NXPE3,NXPE4,NXPH1,NXPH2,NXPH3,NXPH4,NXT1,NXT2,NYAP1,NYAP2,NYNRIN,NYX,OAF,OARD1,OAS1,OAS2,OAS3,OASL,OAT,OAZ1,OAZ2,OAZ3,OBI1,OBP2A,OBP2B,OBSCN,OBSL1,OC90,OCA2,OCEL1,OCIAD1,OCIAD2,OCLN,OCM,OCM2,OCRL,OCSTAMP,ODAD1,ODAD2,ODAD3,ODAD4,ODAM,ODAPH,ODC1,ODF1,ODF2,ODF2L,ODF4,ODR4,OFD1,OGA,OGDH,OGDHL,OGFOD1,OGFOD2,OGFOD3,OGFR,OGFRL1,OGG1,OGN,OGT,OIP5,OIT3,OLA1,OLAH,OLFM1,OLFM2,OLFM3,OLFM4,OLFML1,OLFML2A,OLFML2B,OLFML3,OLIG1,OLIG2,OLIG3,OLMALINC,OLR1,OMA1,OMD,OMG,OMP,ONECUT1,ONECUT2,ONECUT3,OOEP,OOSP2,OPA1,OPA3,OPALIN,OPCML,OPHN1,OPLAH,OPN1LW,OPN1MW,OPN1MW2,OPN1SW,OPN3,OPN4,OPN5,OPRD1,OPRK1,OPRL1,OPRM1,OPRPN,OPTC,OPTN,OR10A2,OR10A3,OR10A4,OR10A5,OR10A6,OR10A7,OR10AD1,OR10AG1,OR10G2,OR10G3,OR10G4,OR10G7,OR10G8,OR10G9,OR10H1,OR10H2,OR10H3,OR10H4,OR10H5,OR10J1,OR10J3,OR10J5,OR10K1,OR10K2,OR10P1,OR10Q1,OR10R2,OR10S1,OR10T2,OR10V1,OR10W1,OR10X1,OR10Z1,OR11G2,OR11H4,OR11H6,OR11L1,OR13A1,OR13C2,OR13C3,OR13C4,OR13C5,OR13C8,OR13C9,OR13D1,OR13F1,OR13H1,OR13J1,OR14A16,OR14C36,OR14I1,OR1A1,OR1A2,OR1B1,OR1C1,OR1D2,OR1D5,OR1E1,OR1E2,OR1F1,OR1F2P,OR1G1,OR1I1,OR1J1,OR1J2,OR1J4,OR1K1,OR1L1,OR1L3,OR1L4,OR1L6,OR1L8,OR1M1,OR1N1,OR1N2,OR1Q1,OR1S1,OR1S2,OR2A12,OR2A14,OR2A2,OR2A25,OR2A4,OR2A5,OR2A7,OR2AE1,OR2AG1,OR2AG2,OR2AK2,OR2AT4,OR2B11,OR2B2,OR2B6,OR2C1,OR2C3,OR2D2,OR2D3,OR2F1,OR2F2,OR2G2,OR2G3,OR2G6,OR2K2,OR2L13,OR2L1P,OR2L2,OR2L3,OR2L8,OR2M1P,OR2M2,OR2M3,OR2M4,OR2M5,OR2M7,OR2S2,OR2T1,OR2T10,OR2T11,OR2T12,OR2T27,OR2T29,OR2T3,OR2T33,OR2T34,OR2T4,OR2T5,OR2T6,OR2T8,OR2V2,OR2W3,OR2W5P,OR2Y1,OR2Z1,OR3A1,OR3A2,OR3A3,OR3A4P,OR4A15,OR4A16,OR4A47,OR4A5,OR4B1,OR4C11,OR4C12,OR4C13,OR4C15,OR4C16,OR4C3,OR4C45,OR4C46,OR4C6,OR4D1,OR4D10,OR4D11,OR4D2,OR4D5,OR4D6,OR4D9,OR4E2,OR4F13P,OR4F15,OR4F6,OR4K1,OR4K13,OR4K14,OR4K15,OR4K17,OR4K2,OR4K5,OR4L1,OR4M2,OR4N2,OR4N3P,OR4N4,OR4N5,OR4P4,OR4Q3,OR4S1,OR4S2,OR4X1,OR4X2,OR51A2,OR51A4,OR51A7,OR51B2,OR51B4,OR51B5,OR51B6,OR51D1,OR51E1,OR51E2,OR51F1,OR51F2,OR51G1,OR51G2,OR51I1,OR51I2,OR51L1,OR51M1,OR51Q1,OR51S1,OR51T1,OR51V1,OR52A1,OR52A4P,OR52A5,OR52B2,OR52B4,OR52B6,OR52D1,OR52E2,OR52E4,OR52E6,OR52E8,OR52H1,OR52I1,OR52I2,OR52J3,OR52K1,OR52K2,OR52L1,OR52M1,OR52N1,OR52N2,OR52N4,OR52N5,OR52R1,OR52W1,OR56A1,OR56A3,OR56A4,OR56A5,OR56B1,OR56B4,OR5A1,OR5A2,OR5AC2,OR5AK2,OR5AK4P,OR5AN1,OR5AP2,OR5AR1,OR5AS1,OR5AU1,OR5B12,OR5B17,OR5B2,OR5B21,OR5B3,OR5C1,OR5D13,OR5D14,OR5D16,OR5D18,OR5E1P,OR5F1,OR5H1,OR5H14,OR5H15,OR5H2,OR5H6,OR5I1,OR5J2,OR5K1,OR5K2,OR5K3,OR5K4,OR5L1,OR5L2,OR5M1,OR5M10,OR5M11,OR5M3,OR5M8,OR5M9,OR5P2,OR5P3,OR5T1,OR5T2,OR5T3,OR5W2,OR6A2,OR6B1,OR6B2,OR6B3,OR6C1,OR6C2,OR6C3,OR6C4,OR6C6,O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diff --git a/p1/preprocess/Mitochondrial_Disorders/clinical_data/GSE30933.csv b/p1/preprocess/Mitochondrial_Disorders/clinical_data/GSE30933.csv new file mode 100644 index 0000000000000000000000000000000000000000..25b3914d3828473d144b9e7e582431de3956defa --- /dev/null +++ b/p1/preprocess/Mitochondrial_Disorders/clinical_data/GSE30933.csv @@ -0,0 +1,2 @@ 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+0.0,1.0,1.0,0.0,0.0,0.0,0.0,1.0,1.0,0.0,0.0,0.0,1.0,0.0,0.0,0.0,1.0,1.0,0.0,0.0,1.0,1.0,0.0,0.0,1.0,0.0,0.0,1.0,0.0,0.0,1.0,1.0,0.0,0.0,1.0,1.0,0.0,0.0,0.0,0.0,1.0,0.0,0.0,0.0,1.0,1.0,0.0,0.0,0.0,0.0,1.0,0.0,0.0 diff --git a/p1/preprocess/Mitochondrial_Disorders/code/GSE22651.py b/p1/preprocess/Mitochondrial_Disorders/code/GSE22651.py new file mode 100644 index 0000000000000000000000000000000000000000..429edb88a36bf216b2b4040c486caadfa4475a79 --- /dev/null +++ b/p1/preprocess/Mitochondrial_Disorders/code/GSE22651.py @@ -0,0 +1,172 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Mitochondrial_Disorders" +cohort = "GSE22651" + +# Input paths +in_trait_dir = "../DATA/GEO/Mitochondrial_Disorders" +in_cohort_dir = "../DATA/GEO/Mitochondrial_Disorders/GSE22651" + +# Output paths +out_data_file = "./output/preprocess/1/Mitochondrial_Disorders/GSE22651.csv" +out_gene_data_file = "./output/preprocess/1/Mitochondrial_Disorders/gene_data/GSE22651.csv" +out_clinical_data_file = "./output/preprocess/1/Mitochondrial_Disorders/clinical_data/GSE22651.csv" +json_path = "./output/preprocess/1/Mitochondrial_Disorders/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1) Gene Expression Data Availability +is_gene_available = True + +# 2) Variable Availability and Data Type Conversion + +# No row mentions "Mitochondrial_Disorders" or a clearly indicated Friedreich’s ataxia label, +# so we still set trait_row to None. +trait_row = None + +# From the dictionary, we only see "age: 47 years" in row 0 (a single unique age), so not useful for analysis. +age_row = None + +# Although we see 'gender: male' in row 0 and 'gender: female' in row 1, +# each row individually has only one unique gender value. Per the step instructions, +# that makes it effectively unavailable. Hence, gender_row = None. +gender_row = None + +def convert_trait(value: str) -> Optional[int]: + """ + Convert the trait string to a chosen data type for analysis. + Since trait data is not available (trait_row=None), return None. + """ + return None + +def convert_age(value: str) -> Optional[float]: + """ + Convert the age string to float if applicable. Since we do not have a valid age row, return None. + """ + return None + +def convert_gender(value: str) -> Optional[int]: + """ + Convert gender string to 0 (female) or 1 (male). If unknown, return None. + Since gender_row is None, this will not be used. + """ + return None + +# 3) Save Metadata (Initial Filtering) +# Trait data availability is determined by trait_row. +is_trait_available = (trait_row is not None) +is_usable = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4) Clinical Feature Extraction +# Skip because trait_row is None (no explicit trait data). +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# These are Illumina probe identifiers (e.g., "ILMN_1651199") and not standard human gene symbols. +# Therefore, we need to perform gene mapping. + +print("requires_gene_mapping = True") +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP: Gene Identifier Mapping + +# 1. Determine the columns in 'gene_annotation' for gene ID and gene symbol +# From the preview, the 'ID' column contains probe identifiers like "ILMN_1725881", +# and the 'Symbol' column typically holds the gene symbol. + +mapping_df = get_gene_mapping(gene_annotation, prob_col='ID', gene_col='Symbol') + +# 2. Convert probe-level measurements to gene expression data using the mapping, +# distributing expression equally among multiple mapped genes and summing up values per gene. + +gene_data = apply_gene_mapping(gene_data, mapping_df) +import os +import pandas as pd + +# STEP 7: Data Normalization and Linking + +# First, check if the clinical CSV file exists. If it does not, we cannot proceed with trait-based linking. +if not os.path.exists(out_clinical_data_file): + # No trait data file => dataset is not usable for trait analysis + df_null = pd.DataFrame() + is_biased = True # Arbitrary boolean to satisfy function requirement + validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=is_biased, + df=df_null, + note="No trait data file found; dataset not usable for trait analysis." + ) + +else: + # 1. Normalize the mapped gene expression data using known gene symbol synonyms, then save. + normalized_gene_data = normalize_gene_symbols_in_index(gene_data) + normalized_gene_data.to_csv(out_gene_data_file) + + # 2. Load the previously extracted clinical CSV. + selected_clinical_df = pd.read_csv(out_clinical_data_file) + # If we had a single-row trait, rename row 0 to the trait name (example usage). + selected_clinical_df = selected_clinical_df.rename(index={0: trait}) + + # Combine these as our final clinical data; in this dataset, we only have trait info (if any). + combined_clinical_df = selected_clinical_df + + # Link the clinical and genetic data by matching sample IDs in columns. + linked_data = geo_link_clinical_genetic_data(combined_clinical_df, normalized_gene_data) + + # 3. Handle missing values in the linked data (drop incomplete rows/columns, then impute). + processed_data = handle_missing_values(linked_data, trait) + + # 4. Check trait bias and remove any biased demographic features (if any). + trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + + # 5. Final validation and metadata saving. + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=processed_data, + note="Completed trait-based preprocessing." + ) + + # 6. If final dataset is usable, save. Otherwise, skip. + if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Mitochondrial_Disorders/code/GSE30933.py b/p1/preprocess/Mitochondrial_Disorders/code/GSE30933.py new file mode 100644 index 0000000000000000000000000000000000000000..e8debd4fc94ff2ecc10c2e13ffdd6387109e314f --- /dev/null +++ b/p1/preprocess/Mitochondrial_Disorders/code/GSE30933.py @@ -0,0 +1,184 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Mitochondrial_Disorders" +cohort = "GSE30933" + +# Input paths +in_trait_dir = "../DATA/GEO/Mitochondrial_Disorders" +in_cohort_dir = "../DATA/GEO/Mitochondrial_Disorders/GSE30933" + +# Output paths +out_data_file = "./output/preprocess/1/Mitochondrial_Disorders/GSE30933.csv" +out_gene_data_file = "./output/preprocess/1/Mitochondrial_Disorders/gene_data/GSE30933.csv" +out_clinical_data_file = "./output/preprocess/1/Mitochondrial_Disorders/clinical_data/GSE30933.csv" +json_path = "./output/preprocess/1/Mitochondrial_Disorders/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1. Gene Expression Data Availability +# Based on the background info "A Gene Expression Phenotype..." this dataset is indeed measuring gene expression. +is_gene_available = True + +# 2. Variable Availability and Data Type Conversion +# From the sample characteristics dictionary, row 0 corresponds to "disease status: Carrier/FRDA/Normal" +# This is our trait variable. There is no mention of age or gender in the dictionary. +trait_row = 0 +age_row = None +gender_row = None + +# Define the conversion functions. +def convert_trait(value: str) -> Optional[int]: + """ + Convert the 'disease status' field to a binary variable: + FRDA = 1, otherwise (Carrier, Normal) = 0. + Unknown or unexpected -> None + """ + # Each field looks like "disease status: FRDA", so split by ':' and take the second part + parts = value.split(':') + if len(parts) < 2: + return None + status = parts[1].strip().lower() + if status == "frda": + return 1 + elif status in ["carrier", "normal"]: + return 0 + return None + +def convert_age(value: str) -> Optional[float]: + """No age data available, always return None.""" + return None + +def convert_gender(value: str) -> Optional[int]: + """No gender data available, always return None.""" + return None + +# 3. Save Metadata (initial filtering) +is_trait_available = trait_row is not None +is_usable = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4. Clinical Feature Extraction (only if trait data is available) +if trait_row is not None: + # Suppose 'clinical_data' is our DataFrame of clinical characteristics + # (it is assumed to be available from previous steps) + selected_clinical_df = geo_select_clinical_features( + clinical_data, + trait=trait, + trait_row=trait_row, + convert_trait=convert_trait, + age_row=age_row, + convert_age=convert_age, + gender_row=gender_row, + convert_gender=convert_gender + ) + + # Preview the extracted DataFrame + preview_dict = preview_df(selected_clinical_df) + print("Preview of selected clinical features:", preview_dict) + + # Save the clinical data + selected_clinical_df.to_csv(out_clinical_data_file, index=False) +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# Observing the gene IDs in the provided list, they appear to be Illumina probe IDs (ILMN_xxxxx). +# These are not human gene symbols and, thus, require mapping to gene symbols. + +requires_gene_mapping = True +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP: Gene Identifier Mapping +# 1. Identify columns for gene identifiers and gene symbols in the annotation dataframe +mapping_df = get_gene_mapping(gene_annotation, prob_col="ID", gene_col="SYMBOL") + +# 2. Apply gene mapping to convert probe-level expression data to gene-level expression data +gene_data = apply_gene_mapping(gene_data, mapping_df) +import os +import pandas as pd + +# STEP 7: Data Normalization and Linking + +# First, check if the clinical CSV file exists. If it does not, we cannot proceed with trait-based linking. +if not os.path.exists(out_clinical_data_file): + # No trait data file => dataset is not usable for trait analysis + df_null = pd.DataFrame() + is_biased = True # Arbitrary boolean to satisfy function requirement + validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=is_biased, + df=df_null, + note="No trait data file found; dataset not usable for trait analysis." + ) + +else: + # 1. Normalize the mapped gene expression data using known gene symbol synonyms, then save. + normalized_gene_data = normalize_gene_symbols_in_index(gene_data) + normalized_gene_data.to_csv(out_gene_data_file) + + # 2. Load the previously extracted clinical CSV. + selected_clinical_df = pd.read_csv(out_clinical_data_file) + # If we had a single-row trait, rename row 0 to the trait name (example usage). + selected_clinical_df = selected_clinical_df.rename(index={0: trait}) + + # Combine these as our final clinical data; in this dataset, we only have trait info (if any). + combined_clinical_df = selected_clinical_df + + # Link the clinical and genetic data by matching sample IDs in columns. + linked_data = geo_link_clinical_genetic_data(combined_clinical_df, normalized_gene_data) + + # 3. Handle missing values in the linked data (drop incomplete rows/columns, then impute). + processed_data = handle_missing_values(linked_data, trait) + + # 4. Check trait bias and remove any biased demographic features (if any). + trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + + # 5. Final validation and metadata saving. + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=processed_data, + note="Completed trait-based preprocessing." + ) + + # 6. If final dataset is usable, save. Otherwise, skip. + if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Mitochondrial_Disorders/code/GSE42986.py b/p1/preprocess/Mitochondrial_Disorders/code/GSE42986.py new file mode 100644 index 0000000000000000000000000000000000000000..4c9dfb546dcfd07be6780cd85ec178cf1ec370b1 --- /dev/null +++ b/p1/preprocess/Mitochondrial_Disorders/code/GSE42986.py @@ -0,0 +1,214 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Mitochondrial_Disorders" +cohort = "GSE42986" + +# Input paths +in_trait_dir = "../DATA/GEO/Mitochondrial_Disorders" +in_cohort_dir = "../DATA/GEO/Mitochondrial_Disorders/GSE42986" + +# Output paths +out_data_file = "./output/preprocess/1/Mitochondrial_Disorders/GSE42986.csv" +out_gene_data_file = "./output/preprocess/1/Mitochondrial_Disorders/gene_data/GSE42986.csv" +out_clinical_data_file = "./output/preprocess/1/Mitochondrial_Disorders/clinical_data/GSE42986.csv" +json_path = "./output/preprocess/1/Mitochondrial_Disorders/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1) Gene Expression Data Availability +is_gene_available = True # Based on the Affymetrix platform mention (Human Exon 1.0 ST arrays), this is gene expression data. + +# 2) Variable Availability +# From the sample characteristics dictionary: +# - trait (Mitochondrial_Disorders) can be inferred from row 4 ("informatic analysis group"), +# which has "Control Group", "Mito Disease Group", etc. +# - age is in row 3 ("age (years): ...") +# - gender is in row 2 ("gender: F" / "gender: M") + +trait_row = 4 +age_row = 3 +gender_row = 2 + +# 2.2 Data Type Conversion Functions + +def convert_trait(x: str): + """ + Convert the string from row 4 into a binary variable: 0 for 'Control Group', 1 for 'Mito Disease Group'. + Excluded or unknown will be mapped to None. + """ + parts = x.split(":", 1) + if len(parts) < 2: + return None + val = parts[1].strip().lower() + if "control group" in val: + return 0 + elif "mito disease group" in val: + return 1 + else: + return None + +def convert_age(x: str): + """ + Convert the string from row 3 into a continuous variable (float). + 'not obtained' or invalid entries are mapped to None. + """ + parts = x.split(":", 1) + if len(parts) < 2: + return None + val = parts[1].strip().lower() + if "not obtained" in val: + return None + try: + return float(val) + except ValueError: + return None + +def convert_gender(x: str): + """ + Convert the string from row 2 into a binary variable: 0 for female (F), 1 for male (M). + Unknown entries will be mapped to None. + """ + parts = x.split(":", 1) + if len(parts) < 2: + return None + val = parts[1].strip().upper() + if val == "F": + return 0 + elif val == "M": + return 1 + else: + return None + +# 3) Initial Filtering and Saving Metadata +is_trait_available = (trait_row is not None) +is_usable = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4) Clinical Feature Extraction (only if trait data is available) +if trait_row is not None: + df_clinical_selected = geo_select_clinical_features( + clinical_df=clinical_data, # Provided from a previous step + trait=trait, + trait_row=trait_row, + convert_trait=convert_trait, + age_row=age_row, + convert_age=convert_age, + gender_row=gender_row, + convert_gender=convert_gender + ) + # Preview the extracted clinical features + clinical_preview = preview_df(df_clinical_selected) + # Save the clinical data to CSV + df_clinical_selected.to_csv(out_clinical_data_file, index=False) +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +# These appear to be Affymetrix probe IDs (e.g., "10000_at"), not official gene symbols. +# Therefore, they require mapping to gene symbols. +print("requires_gene_mapping = True") +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP: Gene Identifier Mapping + +# 1 & 2. Determine the appropriate columns for probe IDs ("ID") and gene symbols ("Symbol"), +# and create a mapping dataframe. +mapping_df = get_gene_mapping( + annotation=gene_annotation, + prob_col="ID", + gene_col="Symbol" +) + +# 3. Convert probe-level measurements to gene-level measurements. +gene_data = apply_gene_mapping( + expression_df=gene_data, + mapping_df=mapping_df +) +import os +import pandas as pd + +# STEP 7: Data Normalization and Linking + +# First, check if the clinical CSV file exists. If it does not, we cannot proceed with trait-based linking. +if not os.path.exists(out_clinical_data_file): + # No trait data file => dataset is not usable for trait analysis + df_null = pd.DataFrame() + is_biased = True # Arbitrary boolean to satisfy function requirement + validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=is_biased, + df=df_null, + note="No trait data file found; dataset not usable for trait analysis." + ) + +else: + # 1. Normalize the mapped gene expression data using known gene symbol synonyms, then save. + normalized_gene_data = normalize_gene_symbols_in_index(gene_data) + normalized_gene_data.to_csv(out_gene_data_file) + + # 2. Load the previously extracted clinical CSV. + selected_clinical_df = pd.read_csv(out_clinical_data_file) + # If we had a single-row trait, rename row 0 to the trait name (example usage). + selected_clinical_df = selected_clinical_df.rename(index={0: trait}) + + # Combine these as our final clinical data; in this dataset, we only have trait info (if any). + combined_clinical_df = selected_clinical_df + + # Link the clinical and genetic data by matching sample IDs in columns. + linked_data = geo_link_clinical_genetic_data(combined_clinical_df, normalized_gene_data) + + # 3. Handle missing values in the linked data (drop incomplete rows/columns, then impute). + processed_data = handle_missing_values(linked_data, trait) + + # 4. Check trait bias and remove any biased demographic features (if any). + trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + + # 5. Final validation and metadata saving. + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=processed_data, + note="Completed trait-based preprocessing." + ) + + # 6. If final dataset is usable, save. Otherwise, skip. + if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Mitochondrial_Disorders/code/GSE65399.py b/p1/preprocess/Mitochondrial_Disorders/code/GSE65399.py new file mode 100644 index 0000000000000000000000000000000000000000..9d1574c9b98ebb7b973655f3904043b25634a421 --- /dev/null +++ b/p1/preprocess/Mitochondrial_Disorders/code/GSE65399.py @@ -0,0 +1,184 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Mitochondrial_Disorders" +cohort = "GSE65399" + +# Input paths +in_trait_dir = "../DATA/GEO/Mitochondrial_Disorders" +in_cohort_dir = "../DATA/GEO/Mitochondrial_Disorders/GSE65399" + +# Output paths +out_data_file = "./output/preprocess/1/Mitochondrial_Disorders/GSE65399.csv" +out_gene_data_file = "./output/preprocess/1/Mitochondrial_Disorders/gene_data/GSE65399.csv" +out_clinical_data_file = "./output/preprocess/1/Mitochondrial_Disorders/clinical_data/GSE65399.csv" +json_path = "./output/preprocess/1/Mitochondrial_Disorders/cohort_info.json" + +# STEP1 +from tools.preprocess import * +# 1. Identify the paths to the SOFT file and the matrix file +soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir) + +# 2. Read the matrix file to obtain background information and sample characteristics data +background_prefixes = ['!Series_title', '!Series_summary', '!Series_overall_design'] +clinical_prefixes = ['!Sample_geo_accession', '!Sample_characteristics_ch1'] +background_info, clinical_data = get_background_and_clinical_data(matrix_file, background_prefixes, clinical_prefixes) + +# 3. Obtain the sample characteristics dictionary from the clinical dataframe +sample_characteristics_dict = get_unique_values_by_row(clinical_data) + +# 4. Explicitly print out all the background information and the sample characteristics dictionary +print("Background Information:") +print(background_info) +print("Sample Characteristics Dictionary:") +print(sample_characteristics_dict) +# 1. Gene expression data availability +is_gene_available = True # Based on "Gene expression profiles ... using the Illumina HT12v4 Gene Expression BeadArray." + +# 2. Variable Availability & Data Type Conversion + +# Observing the sample characteristics: +# Key 0 -> "differentiation or tissue type: ..." +# Key 1 -> "time point: d24", "time point: 20wk", ... +# There's no indication of a disease/trait status in these fields. Hence, trait is not available. +trait_row = None + +# Key 1 appears to represent "time point" in weeks or days, which we can interpret as an 'age' measure. +age_row = 1 + +# There's no mention or indication of gender data. +gender_row = None + +# Define the conversion functions +def convert_trait(x: str) -> int: + # No actual trait data found, return None for all. + return None + +def convert_age(x: str) -> float: + # Expected format: "time point: 20wk", or "time point: d24", etc. + # We'll parse the substring after the colon. + if not isinstance(x, str): + return None + parts = x.split(":") + if len(parts) < 2: + return None + val = parts[1].strip().lower() # e.g. "20wk", "d24" + if val.endswith("wk"): + # remove 'wk' and convert to float + num_str = val.replace("wk", "") + try: + return float(num_str) + except ValueError: + return None + elif val.startswith("d"): + # remove 'd' and convert days to weeks + num_str = val.replace("d", "") + try: + days = float(num_str) + return days / 7.0 + except ValueError: + return None + return None + +def convert_gender(x: str) -> int: + # No gender data found, return None for all. + return None + +# 3. Initial filtering & saving metadata +is_trait_available = (trait_row is not None) +is_usable = validate_and_save_cohort_info( + is_final=False, + cohort=cohort, + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available +) + +# 4. Clinical Feature Extraction +# Only proceed if trait_row is not None. Here, trait_row is None, so we skip. +# STEP3 +# 1. Use the get_genetic_data function from the library to get the gene_data from the matrix_file previously defined. +gene_data = get_genetic_data(matrix_file) + +# 2. Print the first 20 row IDs (gene or probe identifiers) for future observation. +print(gene_data.index[:20]) +print("The gene identifiers in this dataset are Illumina probe IDs, which are not standard human gene symbols and require mapping.") +print("requires_gene_mapping = True") +# STEP5 +# 1. Use the 'get_gene_annotation' function from the library to get gene annotation data from the SOFT file. +gene_annotation = get_gene_annotation(soft_file) + +# 2. Use the 'preview_df' function from the library to preview the data and print out the results. +print("Gene annotation preview:") +print(preview_df(gene_annotation)) +# STEP: Gene Identifier Mapping + +# 1. Identify the relevant columns in the annotation dataframe for probe IDs and gene symbols. +# Here, "ID" matches the Illumina probe IDs (e.g., ILMN_1343048) and "Symbol" holds the gene symbol information. +mapping_df = get_gene_mapping(gene_annotation, prob_col="ID", gene_col="Symbol") + +# 2. Apply the gene mapping to convert from probe-level expression to gene-level expression. +gene_data = apply_gene_mapping(gene_data, mapping_df) + +# (Optional) Display the resulting gene_data shape and a quick index sample for verification. +print("Gene data shape after mapping:", gene_data.shape) +print("First few gene symbols after mapping:", gene_data.index[:10].tolist()) +import os +import pandas as pd + +# STEP 7: Data Normalization and Linking + +# First, check if the clinical CSV file exists. If it does not, we cannot proceed with trait-based linking. +if not os.path.exists(out_clinical_data_file): + # No trait data file => dataset is not usable for trait analysis + df_null = pd.DataFrame() + is_biased = True # Arbitrary boolean to satisfy function requirement + validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=False, + is_biased=is_biased, + df=df_null, + note="No trait data file found; dataset not usable for trait analysis." + ) + +else: + # 1. Normalize the mapped gene expression data using known gene symbol synonyms, then save. + normalized_gene_data = normalize_gene_symbols_in_index(gene_data) + normalized_gene_data.to_csv(out_gene_data_file) + + # 2. Load the previously extracted clinical CSV. + selected_clinical_df = pd.read_csv(out_clinical_data_file) + # If we had a single-row trait, rename row 0 to the trait name (example usage). + selected_clinical_df = selected_clinical_df.rename(index={0: trait}) + + # Combine these as our final clinical data; in this dataset, we only have trait info (if any). + combined_clinical_df = selected_clinical_df + + # Link the clinical and genetic data by matching sample IDs in columns. + linked_data = geo_link_clinical_genetic_data(combined_clinical_df, normalized_gene_data) + + # 3. Handle missing values in the linked data (drop incomplete rows/columns, then impute). + processed_data = handle_missing_values(linked_data, trait) + + # 4. Check trait bias and remove any biased demographic features (if any). + trait_biased, processed_data = judge_and_remove_biased_features(processed_data, trait) + + # 5. Final validation and metadata saving. + is_usable = validate_and_save_cohort_info( + is_final=True, + cohort=cohort, + info_path=json_path, + is_gene_available=True, + is_trait_available=True, + is_biased=trait_biased, + df=processed_data, + note="Completed trait-based preprocessing." + ) + + # 6. If final dataset is usable, save. Otherwise, skip. + if is_usable: + processed_data.to_csv(out_data_file) \ No newline at end of file diff --git a/p1/preprocess/Mitochondrial_Disorders/code/TCGA.py b/p1/preprocess/Mitochondrial_Disorders/code/TCGA.py new file mode 100644 index 0000000000000000000000000000000000000000..b73bef28c20e7d1af2a21d1b33e78dff535420eb --- /dev/null +++ b/p1/preprocess/Mitochondrial_Disorders/code/TCGA.py @@ -0,0 +1,70 @@ +# Path Configuration +from tools.preprocess import * + +# Processing context +trait = "Mitochondrial_Disorders" + +# Input paths +tcga_root_dir = "../DATA/TCGA" + +# Output paths +out_data_file = "./output/preprocess/1/Mitochondrial_Disorders/TCGA.csv" +out_gene_data_file = "./output/preprocess/1/Mitochondrial_Disorders/gene_data/TCGA.csv" +out_clinical_data_file = "./output/preprocess/1/Mitochondrial_Disorders/clinical_data/TCGA.csv" +json_path = "./output/preprocess/1/Mitochondrial_Disorders/cohort_info.json" + +import os +import pandas as pd + +# List of subdirectories provided in the instructions: +subdirectories = [ + 'CrawlData.ipynb', '.DS_Store', 'TCGA_lower_grade_glioma_and_glioblastoma_(GBMLGG)', + 'TCGA_Uterine_Carcinosarcoma_(UCS)', 'TCGA_Thyroid_Cancer_(THCA)', 'TCGA_Thymoma_(THYM)', + 'TCGA_Testicular_Cancer_(TGCT)', 'TCGA_Stomach_Cancer_(STAD)', 'TCGA_Sarcoma_(SARC)', + 'TCGA_Rectal_Cancer_(READ)', 'TCGA_Prostate_Cancer_(PRAD)', 'TCGA_Pheochromocytoma_Paraganglioma_(PCPG)', + 'TCGA_Pancreatic_Cancer_(PAAD)', 'TCGA_Ovarian_Cancer_(OV)', 'TCGA_Ocular_melanomas_(UVM)', + 'TCGA_Mesothelioma_(MESO)', 'TCGA_Melanoma_(SKCM)', 'TCGA_Lung_Squamous_Cell_Carcinoma_(LUSC)', + 'TCGA_Lung_Cancer_(LUNG)', 'TCGA_Lung_Adenocarcinoma_(LUAD)', 'TCGA_Lower_Grade_Glioma_(LGG)', + 'TCGA_Liver_Cancer_(LIHC)', 'TCGA_Large_Bcell_Lymphoma_(DLBC)', 'TCGA_Kidney_Papillary_Cell_Carcinoma_(KIRP)', + 'TCGA_Kidney_Clear_Cell_Carcinoma_(KIRC)', 'TCGA_Kidney_Chromophobe_(KICH)', 'TCGA_Head_and_Neck_Cancer_(HNSC)', + 'TCGA_Glioblastoma_(GBM)', 'TCGA_Esophageal_Cancer_(ESCA)', 'TCGA_Endometrioid_Cancer_(UCEC)', + 'TCGA_Colon_and_Rectal_Cancer_(COADREAD)', 'TCGA_Colon_Cancer_(COAD)', 'TCGA_Cervical_Cancer_(CESC)', + 'TCGA_Breast_Cancer_(BRCA)', 'TCGA_Bladder_Cancer_(BLCA)', 'TCGA_Bile_Duct_Cancer_(CHOL)', + 'TCGA_Adrenocortical_Cancer_(ACC)', 'TCGA_Acute_Myeloid_Leukemia_(LAML)' +] + +# Keywords/synonyms related to "Mitochondrial_Disorders" +mito_synonyms = ["mito", "mitochondrial", "mitochondria", "mitochondrial_disorder"] + +selected_subdirectory = None +for subdir in subdirectories: + subdir_lower = subdir.lower() + if any(syn in subdir_lower for syn in mito_synonyms): + selected_subdirectory = subdir + break + +if not selected_subdirectory: + # If no matching directory is found, mark dataset as unavailable + is_final = False + is_gene_available = False + is_trait_available = False + _ = validate_and_save_cohort_info( + is_final=is_final, + cohort="TCGA", + info_path=json_path, + is_gene_available=is_gene_available, + is_trait_available=is_trait_available + ) + print(f"No suitable directory found for '{trait}'. Skipped this trait.") +else: + # Step 2: Identify clinicalMatrix file and PANCAN file + cohort_dir = os.path.join(tcga_root_dir, selected_subdirectory) + clinical_file_path, genetic_file_path = tcga_get_relevant_filepaths(cohort_dir) + + # Step 3: Load both files as dataframes + clinical_df = pd.read_csv(clinical_file_path, index_col=0, sep='\t') + genetic_df = pd.read_csv(genetic_file_path, index_col=0, sep='\t') + + # Step 4: Print the column names of the clinical data + print("Clinical data columns:") + print(list(clinical_df.columns)) \ No newline at end of file diff --git a/p1/preprocess/Mitochondrial_Disorders/cohort_info.json b/p1/preprocess/Mitochondrial_Disorders/cohort_info.json new file mode 100644 index 0000000000000000000000000000000000000000..8e272d4dd4304ff276085d9a08def062f1b2e57d --- /dev/null +++ b/p1/preprocess/Mitochondrial_Disorders/cohort_info.json @@ -0,0 +1 @@ +{"GSE65399": {"is_usable": false, "is_gene_available": false, "is_trait_available": false, "is_available": false, "is_biased": null, "has_age": null, "has_gender": null, "sample_size": null, "note": "No trait data file found; dataset not usable for trait analysis."}, "GSE42986": {"is_usable": true, "is_gene_available": true, "is_trait_available": true, "is_available": true, "is_biased": false, "has_age": false, "has_gender": false, "sample_size": 43, "note": "Completed trait-based preprocessing."}, "GSE30933": {"is_usable": true, "is_gene_available": true, "is_trait_available": true, "is_available": true, "is_biased": false, "has_age": false, "has_gender": false, "sample_size": 108, "note": "Completed trait-based preprocessing."}, "GSE22651": {"is_usable": false, "is_gene_available": false, "is_trait_available": false, "is_available": false, "is_biased": null, "has_age": null, "has_gender": null, "sample_size": null, "note": "No trait data file found; dataset not usable for trait analysis."}, "TCGA": {"is_usable": false, "is_gene_available": false, "is_trait_available": false, "is_available": false, "is_biased": null, "has_age": null, "has_gender": null, "sample_size": null, "note": null}} \ No newline at end of file diff --git a/p1/preprocess/Multiple_Endocrine_Neoplasia_Type_2/clinical_data/GSE19987.csv b/p1/preprocess/Multiple_Endocrine_Neoplasia_Type_2/clinical_data/GSE19987.csv new file mode 100644 index 0000000000000000000000000000000000000000..eedd2a3de037380b11b787ebd4a978f08528bcc6 --- /dev/null +++ b/p1/preprocess/Multiple_Endocrine_Neoplasia_Type_2/clinical_data/GSE19987.csv @@ -0,0 +1,2 @@ +GSM62248,GSM62249,GSM62250,GSM62251,GSM62252,GSM62253,GSM62254,GSM62255,GSM62256,GSM62257,GSM62258,GSM62259,GSM62260,GSM62261,GSM62262,GSM62263,GSM62264,GSM62265,GSM62266,GSM62267,GSM62268,GSM62269,GSM62270,GSM62271,GSM62272,GSM62273,GSM62274,GSM62275,GSM62276,GSM62277,GSM62278,GSM62279,GSM62280,GSM62281,GSM62282,GSM62283,GSM62284,GSM62285,GSM62286,GSM62287,GSM62288,GSM62289,GSM62290,GSM62291,GSM62292,GSM62293,GSM62294,GSM62295,GSM62296,GSM62297,GSM62298,GSM62299,GSM62300,GSM62301,GSM62302,GSM62303,GSM62304,GSM62305,GSM62306,GSM62307,GSM62308,GSM62309,GSM62310,GSM62311,GSM62312,GSM62313,GSM62314,GSM62315,GSM62316,GSM62317,GSM62318,GSM62319,GSM62320,GSM62321,GSM62322 +1.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,1.0,0.0,0.0,1.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,1.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,1.0,0.0,0.0,0.0,1.0,0.0,0.0,0.0,0.0,1.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,1.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,0.0,1.0,0.0,0.0,0.0,0.0,0.0,0.0 diff --git a/p1/preprocess/Multiple_Endocrine_Neoplasia_Type_2/cohort_info.json b/p1/preprocess/Multiple_Endocrine_Neoplasia_Type_2/cohort_info.json new file mode 100644 index 0000000000000000000000000000000000000000..3571841c6185d71c7aad716b85762b0379683499 --- /dev/null +++ b/p1/preprocess/Multiple_Endocrine_Neoplasia_Type_2/cohort_info.json @@ -0,0 +1 @@ +{"GSE19987": {"is_usable": true, "is_gene_available": true, "is_trait_available": true, "is_available": true, "is_biased": false, "has_age": false, "has_gender": false, "sample_size": 75, "note": "Completed trait-based preprocessing."}} \ No newline at end of file diff --git a/p1/preprocess/lower_grade_glioma_and_glioblastoma/gene_data/GSE35158.csv b/p1/preprocess/lower_grade_glioma_and_glioblastoma/gene_data/GSE35158.csv new file mode 100644 index 0000000000000000000000000000000000000000..0ca0aeec0dc555b922b313218865c864891b9871 --- /dev/null +++ b/p1/preprocess/lower_grade_glioma_and_glioblastoma/gene_data/GSE35158.csv @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:31a56ec47b70442242e56b75ae792ea031c6450fbee615649d0a1b1550930085 +size 12648522