# Path Configuration
from tools.preprocess import *

# Processing context
trait = "Endometrioid_Cancer"
cohort = "GSE68600"

# Input paths
in_trait_dir = "../DATA/GEO/Endometrioid_Cancer"
in_cohort_dir = "../DATA/GEO/Endometrioid_Cancer/GSE68600"

# Output paths
out_data_file = "./output/preprocess/3/Endometrioid_Cancer/GSE68600.csv"
out_gene_data_file = "./output/preprocess/3/Endometrioid_Cancer/gene_data/GSE68600.csv"
out_clinical_data_file = "./output/preprocess/3/Endometrioid_Cancer/clinical_data/GSE68600.csv"
json_path = "./output/preprocess/3/Endometrioid_Cancer/cohort_info.json"

# Get paths to the SOFT and matrix files
soft_file, matrix_file = geo_get_relevant_filepaths(in_cohort_dir)

# Get background info and clinical data from matrix file
background_info, clinical_data = get_background_and_clinical_data(matrix_file)

# Get unique values for each feature (row) in clinical data 
unique_values_dict = get_unique_values_by_row(clinical_data)

# Print background info
print("=== Dataset Background Information ===")
print(background_info)
print("\n=== Sample Characteristics ===")
print(json.dumps(unique_values_dict, indent=2))
# Gene expression data availability - Yes, as indicated by !Series_title about gene expression data and Affymetrix array
is_gene_available = True

# Variable availability
# Trait - Row 4 contains histological types. Endometrioid type indicates trait presence
trait_row = 4

# Age - Not available in sample characteristics 
age_row = None 

# Gender - Row 0 contains gender info but all samples are female (F), so not useful
gender_row = None

def convert_trait(value):
    """Convert histology type to binary for endometrioid cancer"""
    if pd.isna(value) or not isinstance(value, str):
        return None
    value = value.lower().split(": ")[-1]
    # Positive if endometrioid is mentioned in histology
    if "endometrioid" in value:
        return 1
    # Other histology types are negative
    return 0

# Age conversion function not needed since age data unavailable
convert_age = None

# Gender conversion function not needed since all samples are female
convert_gender = None

# Save metadata - is_trait_available determined by trait_row being not None
is_trait_available = trait_row is not None
validate_and_save_cohort_info(
    is_final=False,
    cohort=cohort,
    info_path=json_path,
    is_gene_available=is_gene_available,
    is_trait_available=is_trait_available
)

# Extract clinical features since trait data is available
clinical_df = geo_select_clinical_features(
    clinical_data,
    trait=trait,
    trait_row=trait_row,
    convert_trait=convert_trait,
    age_row=age_row,
    convert_age=convert_age,
    gender_row=gender_row,
    convert_gender=convert_gender
)

# Preview extracted clinical data
preview_dict = preview_df(clinical_df)
print("Preview of clinical data:")
print(preview_dict)

# Save clinical data
clinical_df.to_csv(out_clinical_data_file)
# Extract gene expression data from matrix file
genetic_df = get_genetic_data(matrix_file)

# Print DataFrame shape and first 20 row IDs
print("DataFrame shape:", genetic_df.shape)
print("\nFirst 20 row IDs:")
print(genetic_df.index[:20])

print("\nPreview of first few rows and columns:")
print(genetic_df.head().iloc[:, :5])
# The identifiers like 'A28102_at', 'AB000114_at' etc. appear to be Affymetrix probe IDs
# rather than human gene symbols. These will need to be mapped to standard gene symbols.
requires_gene_mapping = True
# Extract gene annotation data, excluding control probe lines
gene_metadata = get_gene_annotation(soft_file) 

# Preview filtered annotation data
print("Column names:")
print(gene_metadata.columns)
print("\nPreview of gene annotation data:")
print(preview_df(gene_metadata))
# Get gene mapping dataframe from annotation data
# 'ID' stores probe IDs matching gene expression data
# 'Gene Symbol' stores corresponding gene symbols
mapping_df = get_gene_mapping(gene_metadata, prob_col='ID', gene_col='Gene Symbol')

# Apply gene mapping to convert probe-level data to gene expression data
gene_data = apply_gene_mapping(genetic_df, mapping_df)

# Print shape of gene expression data after mapping
print("Gene expression data shape after mapping:", gene_data.shape)
print("\nPreview of first few rows and columns:")
print(gene_data.iloc[:5, :5])
# 1. Normalize gene symbols and save
gene_data = normalize_gene_symbols_in_index(gene_data)
os.makedirs(os.path.dirname(out_gene_data_file), exist_ok=True)
gene_data.to_csv(out_gene_data_file)

# 2. Link clinical and genetic data
clinical_df = pd.read_csv(out_clinical_data_file, index_col=0)
linked_data = geo_link_clinical_genetic_data(clinical_df, gene_data)

# 3. Handle missing values 
linked_data = handle_missing_values(linked_data, trait)

# 4. Check for biased features
trait_biased, linked_data = judge_and_remove_biased_features(linked_data, trait)

# 5. Final validation and metadata saving
is_usable = validate_and_save_cohort_info(
    is_final=True, 
    cohort=cohort,
    info_path=json_path,
    is_gene_available=True,
    is_trait_available=True, 
    is_biased=trait_biased,
    df=linked_data,
    note="Study comparing ERα-chromatin interactions in endometrial tumors from patients with/without tamoxifen treatment history"
)

# 6. Save linked data if usable
if is_usable:
    os.makedirs(os.path.dirname(out_data_file), exist_ok=True)
    linked_data.to_csv(out_data_file)