# Path Configuration
from tools.preprocess import *

# Processing context
trait = "Intellectual_Disability"
cohort = "GSE89594"

# Input paths
in_trait_dir = "../DATA/GEO/Intellectual_Disability"
in_cohort_dir = "../DATA/GEO/Intellectual_Disability/GSE89594"

# Output paths
out_data_file = "./output/preprocess/3/Intellectual_Disability/GSE89594.csv"
out_gene_data_file = "./output/preprocess/3/Intellectual_Disability/gene_data/GSE89594.csv"
out_clinical_data_file = "./output/preprocess/3/Intellectual_Disability/clinical_data/GSE89594.csv"
json_path = "./output/preprocess/3/Intellectual_Disability/cohort_info.json"

# Get file paths
soft_file_path, matrix_file_path = geo_get_relevant_filepaths(in_cohort_dir)

# Get background info and clinical data
background_info, clinical_data = get_background_and_clinical_data(matrix_file_path)

# Get unique values for each clinical feature 
unique_values_dict = get_unique_values_by_row(clinical_data)

# Print background information
print("Background Information:")
print(background_info)
print("\nSample Characteristics:")
print(json.dumps(unique_values_dict, indent=2))
# 1. Gene Expression Data Availability
# Based on background info, this is a gene expression study using peripheral blood
is_gene_available = True

# 2.1 Data Availability
trait_row = 0  # "diagnosis" in row 0 contains trait info
age_row = 2    # "age" in row 2
gender_row = 3  # "gender" in row 3

# 2.2 Data Type Conversion Functions
def convert_trait(x):
    """Convert trait status to binary"""
    if not isinstance(x, str):
        return None
    value = x.split(': ')[-1].lower()
    # Williams Syndrome is intellectual disability
    if 'williams syndrome' in value or 'ws' in value:
        return 1
    elif 'control' in value:
        return 0
    # ASD samples counted as None since not relevant
    return None

def convert_age(x):
    """Convert age to continuous values"""
    if not isinstance(x, str):
        return None
    value = x.split(': ')[-1].lower()
    try:
        # Extract numeric value before 'y'
        return float(value.replace('y',''))
    except:
        return None

def convert_gender(x):
    """Convert gender to binary (0=female, 1=male)"""
    if not isinstance(x, str):
        return None
    value = x.split(': ')[-1].lower()
    if 'female' in value:
        return 0
    elif 'male' in value:
        return 1
    return None

# 3. Save Metadata  
is_trait_available = trait_row is not None
validate_and_save_cohort_info(is_final=False, 
                            cohort=cohort,
                            info_path=json_path,
                            is_gene_available=is_gene_available,
                            is_trait_available=is_trait_available)

# 4. Clinical Feature Extraction
if trait_row is not None:
    clinical_features = geo_select_clinical_features(
        clinical_df=clinical_data,
        trait=trait,
        trait_row=trait_row,
        convert_trait=convert_trait,
        age_row=age_row,
        convert_age=convert_age, 
        gender_row=gender_row,
        convert_gender=convert_gender
    )
    
    # Preview the processed clinical data
    preview = preview_df(clinical_features)
    print("Preview of processed clinical data:")
    print(preview)
    
    # Save clinical features
    clinical_features.to_csv(out_clinical_data_file)
# Extract gene expression data from the matrix file
genetic_data = get_genetic_data(matrix_file_path)

# Print first 20 row IDs
print("First 20 row IDs:")
print(genetic_data.index[:20].tolist())
# Based on the provided sample row IDs which are just sequential numbers, 
# we need to map these identifiers to proper gene symbols
requires_gene_mapping = True
# Extract gene annotation data from SOFT file
gene_metadata = get_gene_annotation(soft_file_path)

# Display information about the annotation data
print("Column names:")
print(gene_metadata.columns.tolist())

# Look at general data statistics 
print("\nData shape:", gene_metadata.shape)

# Display non-NaN value counts for key gene identifier columns
print("\nNumber of non-NaN values in key columns:")
for col in ['ID', 'GENE_SYMBOL']:
    print(f"{col}: {gene_metadata[col].notna().sum()}")

# Preview rows with actual gene information
print("\nPreview of rows with gene information:")
gene_rows = gene_metadata[gene_metadata['GENE_SYMBOL'].notna()].head()
print(json.dumps(preview_df(gene_rows), indent=2))
# 1. Identify mapping columns
# ID in expression data corresponds to ID in annotation
# GENE_SYMBOL contains gene symbols for mapping
mapping_df = get_gene_mapping(gene_metadata, prob_col='ID', gene_col='GENE_SYMBOL')

# 2. Apply mapping and aggregate to get gene expression data 
gene_data = apply_gene_mapping(genetic_data, mapping_df)

# Preview the processed data
print("\nPreview of mapped gene expression data:")
print(f"Shape: {gene_data.shape}")
print("\nFirst few gene symbols:")
print(gene_data.index[:10].tolist())
# 1. Normalize gene symbols
gene_data = normalize_gene_symbols_in_index(gene_data)
gene_data.to_csv(out_gene_data_file)

# Get clinical features 
clinical_features = geo_select_clinical_features(
    clinical_data,
    trait=trait,
    trait_row=trait_row,
    convert_trait=convert_trait,
    age_row=age_row,
    convert_age=convert_age,
    gender_row=gender_row,
    convert_gender=convert_gender
)

# 2. Link clinical and genetic data
linked_data = geo_link_clinical_genetic_data(clinical_features, gene_data)

# 3. Handle missing values
linked_data = handle_missing_values(linked_data, trait)

# Early exit if trait values are all NaN
if linked_data[trait].isna().all():
    is_biased = True
    linked_data = None
else:
    # 4. Judge whether features are biased and remove biased demographic features
    is_biased, linked_data = judge_and_remove_biased_features(linked_data, trait)

# 5. Final validation and save metadata
note = "Dataset contains gene expression data from pediatric AML samples, focusing on Down syndrome cases versus other AML types."
is_usable = validate_and_save_cohort_info(
    is_final=True,
    cohort=cohort,
    info_path=json_path,
    is_gene_available=True,
    is_trait_available=True,
    is_biased=is_biased,
    df=linked_data,
    note=note
)

# 6. Save the linked data only if it's usable
if is_usable:
    os.makedirs(os.path.dirname(out_data_file), exist_ok=True)
    linked_data.to_csv(out_data_file)