# Path Configuration from tools.preprocess import * # Processing context trait = "Stomach_Cancer" cohort = "GSE208099" # Input paths in_trait_dir = "../DATA/GEO/Stomach_Cancer" in_cohort_dir = "../DATA/GEO/Stomach_Cancer/GSE208099" # Output paths out_data_file = "./output/preprocess/3/Stomach_Cancer/GSE208099.csv" out_gene_data_file = "./output/preprocess/3/Stomach_Cancer/gene_data/GSE208099.csv" out_clinical_data_file = "./output/preprocess/3/Stomach_Cancer/clinical_data/GSE208099.csv" json_path = "./output/preprocess/3/Stomach_Cancer/cohort_info.json" # Get file paths soft_file_path, matrix_file_path = geo_get_relevant_filepaths(in_cohort_dir) # Get background info and clinical data background_info, clinical_data = get_background_and_clinical_data(matrix_file_path) print("Background Information:") print(background_info) print("\nSample Characteristics:") # Get dictionary of unique values per row unique_values_dict = get_unique_values_by_row(clinical_data) for row, values in unique_values_dict.items(): print(f"\n{row}:") print(values) # 1. Gene Expression Data Availability is_gene_available = True # Based on background info mentioning "gene expression analysis" # 2.1 Data Availability trait_row = 1 # From tissue field - we can infer cancer status age_row = None # Age not available gender_row = 0 # Gender explicitly recorded # 2.2 Data Type Conversion Functions def convert_trait(x): """Convert tissue type to binary cancer status""" if not isinstance(x, str): return None x = x.lower().split(': ')[-1] if 'adenocarcinoma' in x: return 1 # Cancer elif 'normal' in x: return 0 # Non-cancer return None def convert_gender(x): """Convert gender to binary (0=female, 1=male)""" if not isinstance(x, str): return None x = x.lower().split(': ')[-1] if x == 'f': return 0 elif x == 'm': return 1 return None # 3. Save initial metadata validate_and_save_cohort_info( is_final=False, cohort=cohort, info_path=json_path, is_gene_available=is_gene_available, is_trait_available=(trait_row is not None) ) # 4. Extract clinical features clinical_df = geo_select_clinical_features( clinical_df=clinical_data, trait=trait, trait_row=trait_row, convert_trait=convert_trait, gender_row=gender_row, convert_gender=convert_gender ) # Preview results print(preview_df(clinical_df)) # Save clinical data os.makedirs(os.path.dirname(out_clinical_data_file), exist_ok=True) clinical_df.to_csv(out_clinical_data_file) # Get gene expression data from matrix file genetic_data = get_genetic_data(matrix_file_path) # Examine data structure print("Data structure and head:") print(genetic_data.head()) print("\nShape:", genetic_data.shape) print("\nFirst 20 row IDs (gene/probe identifiers):") print(list(genetic_data.index)[:20]) # Get a few column names to verify sample IDs print("\nFirst 5 column names:") print(list(genetic_data.columns)[:5]) # Based on the prefix 'A_19_P' in row IDs, these are Agilent microarray probe IDs # They need to be mapped to human gene symbols for analysis requires_gene_mapping = True # Extract gene annotation data gene_annotation = get_gene_annotation(soft_file_path) # Preview column names and values from annotation dataframe print("Gene annotation DataFrame preview:") print(preview_df(gene_annotation)) # 1 & 2. Create mapping dataframe from gene annotation # 'ID' contains probe IDs matching gene expression data # 'GENE_SYMBOL' contains the human gene symbols mapping_df = get_gene_mapping(gene_annotation, 'ID', 'GENE_SYMBOL') # 3. Apply gene mapping to convert probe data to gene expression data gene_data = apply_gene_mapping(genetic_data, mapping_df) # Preview result print("\nGene expression data shape after mapping:", gene_data.shape) print("\nFirst few gene symbols:") print(list(gene_data.index)[:10]) # Save gene expression data os.makedirs(os.path.dirname(out_gene_data_file), exist_ok=True) gene_data.to_csv(out_gene_data_file) # 1. Normalize gene symbols in gene expression data gene_data = normalize_gene_symbols_in_index(gene_data) os.makedirs(os.path.dirname(out_gene_data_file), exist_ok=True) gene_data.to_csv(out_gene_data_file) print("\nGene data shape (normalized gene-level):", gene_data.shape) # Load clinical data previously processed selected_clinical_df = pd.read_csv(out_clinical_data_file, index_col=0) print("\nClinical data shape:", selected_clinical_df.shape) # 2. Link clinical and genetic data using normalized gene-level data linked_data = geo_link_clinical_genetic_data(selected_clinical_df, gene_data) print("\nLinked data shape:", linked_data.shape) # 3. Handle missing values systematically if trait in linked_data.columns: linked_data = handle_missing_values(linked_data, trait) # 4. Check for bias in trait and demographic features trait_biased, linked_data = judge_and_remove_biased_features(linked_data, trait) # 5. Final validation and information saving note = "Data was successfully preprocessed from probe-level to gene-level expression using gene symbol normalization with NCBI Gene database." is_usable = validate_and_save_cohort_info( is_final=True, cohort=cohort, info_path=json_path, is_gene_available=True, is_trait_available=True, is_biased=trait_biased, df=linked_data, note=note ) # 6. Save linked data only if usable and not biased if is_usable and not trait_biased: os.makedirs(os.path.dirname(out_data_file), exist_ok=True) linked_data.to_csv(out_data_file)