text_1: New drugs for tuberculosis often do not penetrate the necrotic portion of a tuberculosis lesion in high concentrations.
text_2: Using a matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging suite in a biosafety containment facility, we show that the key sterilizing drugs rifampicin and pyrazinamide efficiently penetrate the sites of TB infection in lung lesions.