Dataset Card for BioGRID-Conv

Dataset Description

BioGRID-Conv is a conversational dataset for protein interaction network prediction tasks, derived from the BioGRID 4.4.246 database. The dataset transforms protein interaction data into structured conversation formats suitable for training language models on biological network prediction and completion tasks, enhanced with comprehensive protein sequence and structural information from UniProt and AlphaFold databases.

This dataset was created by Proximile LLC, a company specializing in secure, on-premise AI solutions for small and medium-sized businesses.

Dataset Summary

This dataset contains conversational examples for training models to predict, complete, and analyze protein interaction networks using both interaction data and detailed protein characteristics. Each conversation follows a structured format with system instructions, user queries about protein networks (including sequence and structural context), and detailed network predictions as responses.

The dataset is built from BioGRID 4.4.246, which contains 2,851,050 protein and genetic interactions from 86,799 publications across major model organism species. Each protein is enriched with:

• UniProt sequence data: Protein sequences, functional annotations, organism information
• AlphaFold structural data: 3D structure availability, confidence scores, coverage information
• Integrated biological context: Combining interaction, sequence, and structural information for enhanced predictions

Supported Tasks

• Sequence-Aware Network Prediction: Given proteins with sequence/structure info, predict all likely interactions
• Structure-Guided Network Completion: Complete partial networks using structural compatibility
• New Protein Integration: Predict interactions for new proteins based on sequence similarity and structural features
• Context-Rich Network Generation: Generate networks with detailed protein characteristics and constraints
• Multi-Modal Biological Reasoning: Combine interaction patterns with sequence and structural data

Languages

English (scientific/biological terminology)

Dataset Structure

Data Instances

Each instance contains a conversation with three components, now enhanced with protein sequence and structural context:

{
  "updated": [
    {
      "role": "system",
      "content": "You are a protein interaction prediction system. Given a list of proteins with their sequence and structural information, predict all likely interactions between them based on biological knowledge, sequence similarity, and structural compatibility."
    },
    {
      "role": "user", 
      "content": "Predict the protein interaction network for these proteins:\n\nPROTEIN: CCKLR-17D1\n  UniProt ID: P12345\n  Full Name: Cholecystokinin-like receptor\n  Organism: Drosophila melanogaster\n  Sequence Length: 389 amino acids\n  Sequence: MSLVLVLLLVLVLVLVLVLVLVLVLVLVLVLVLVLVLVLVLVLVLVLVLV...\n  AlphaFold Structure: Available\n  Structure Confidence: High\n  Function: G-protein coupled receptor involved in...\n\nPROTEIN: CG8507\n  UniProt ID: Q67890\n  Sequence Length: 256 amino acids\n  AlphaFold Structure: Not available\n  Function: Putative kinase involved in signal transduction..."
    },
    {
      "role": "assistant",
      "content": "PROTEINS: CCKLR-17D1, CG8507, CG9928, Dsk, Ptr\n\nTWO-HYBRID INTERACTIONS:\n  CG8507--Dsk\n  CG9928--Dsk\n  Dsk--Ptr\n\nPHENOTYPIC SUPPRESSION INTERACTIONS:\n  CCKLR-17D1--Dsk\n\nNETWORK SUMMARY: 5 proteins, 4 interactions\n\nPROTEIN DETAILS:\n  CCKLR-17D1 | 389aa | AlphaFold(High) | Drosophila\n  CG8507 | 256aa | Drosophila\n  CG9928 | 198aa | AlphaFold(Medium) | Drosophila\n  Dsk | 445aa | AlphaFold(High) | Drosophila\n  Ptr | 312aa | AlphaFold(Low) | Drosophila"
    }
  ]
}

Data Fields

• role: String indicating the conversation participant ("system", "user", "assistant")
• content: String containing the message content
  • System messages define the task type and model behavior, emphasizing sequence/structure integration
  • User messages contain protein lists with detailed biological context, partial networks, or generation constraints
  • Assistant messages contain structured network predictions with protein summaries

Enhanced Network Format

Networks are represented in a standardized text format with integrated protein information:

PROTEINS: [comma-separated list]

[INTERACTION_TYPE] INTERACTIONS:
  protein1--protein2
  protein3--protein4

NETWORK SUMMARY: X proteins, Y interactions

PROTEIN DETAILS:
  protein1 | [length]aa | AlphaFold([confidence]) | [organism]
  protein2 | [length]aa | [organism]

Protein Enrichment Details

Each protein includes when available:

• UniProt ID and cross-references
• Complete amino acid sequences (truncated for display)
• Sequence lengths and composition
• AlphaFold structure availability and confidence scores
• Functional annotations and GO terms
• Organism and taxonomic information
• Structural coverage and model quality metrics

Interaction types include:

• TWO-HYBRID INTERACTIONS
• AFFINITY CAPTURE-MS INTERACTIONS
• AFFINITY CAPTURE-WESTERN INTERACTIONS
• PHENOTYPIC ENHANCEMENT/SUPPRESSION INTERACTIONS
• CO-FRACTIONATION INTERACTIONS
• BIOCHEMICAL ACTIVITY INTERACTIONS
• And others from BioGRID evidence codes

Dataset Creation

Source Data

The dataset is derived from multiple high-quality biological databases:

1. BioGRID 4.4.246: Comprehensive database of protein, genetic and chemical interactions curated from biomedical literature
2. UniProt: Protein sequence database providing functional annotations, sequences, and cross-references
3. AlphaFold: AI-predicted protein structure database from DeepMind/EMBL-EBI

Data Processing Pipeline

The enhanced dataset creation process includes:

1. BioGRID Processing: Neighborhood extraction around high-degree proteins (3-15 connections) to create manageable training examples

2. Protein Data Enrichment:

   • UniProt API Integration: Fetch protein sequences, functional annotations, organism information
   • AlphaFold API Integration: Retrieve structure availability, confidence scores, coverage data
   • Thread-Safe Caching: Persistent file-based caching with file locking to prevent data corruption during parallel processing
   • Multiprocessing: Parallel enrichment of protein data with automatic cache management

3. Enhanced Conversation Generation: Four types of conversational tasks generated from each neighborhood:

   • Complete network prediction from protein lists with sequence context
   • New protein integration using sequence similarity and structural compatibility
   • Partial network completion with structural guidance
   • Property-constrained network generation with biological context

4. Format Standardization: All interactions and protein data converted to consistent text representation with standardized naming and comprehensive biological context

Processing Performance

• Multiprocessing: Utilizes all available CPU cores for parallel processing of neighborhoods, protein enrichment, and conversation generation
• Intelligent Caching: Thread-safe persistent caching reduces API calls by >90% on subsequent runs
• Scalable Architecture: Configurable protein processing limits (default: 5,000 proteins) for memory management
• Batch Processing: Efficient batching of API requests with rate limiting to respect service limits

Data Splits

The dataset contains conversations generated from protein neighborhoods, randomly shuffled. Users should create their own train/validation/test splits as needed for their specific use case.

Considerations for Using the Data

Recommended Use Cases

• Training language models for sequence-aware biological network prediction
• Fine-tuning protein interaction prediction systems with structural context
• Developing conversation agents for integrated bioinformatics analysis
• Benchmarking multi-modal network completion algorithms
• Educational tools combining protein structure, sequence, and interaction analysis
• Research in protein function prediction using multiple data modalities

Enhanced Capabilities

• Multi-Modal Learning: Combines interaction patterns with sequence and structural information
• Biological Realism: Predictions grounded in actual protein characteristics rather than just names
• Structural Awareness: Integration of AlphaFold confidence scores for structure-guided predictions
• Functional Context: UniProt functional annotations provide biological reasoning for interactions
• Scalable Processing: Efficient parallel processing with persistent caching for large-scale analysis

Limitations

• Coverage: Limited to proteins present in BioGRID 4.4.246, UniProt, and AlphaFold databases
• API Dependencies: Protein enrichment requires internet access for initial data fetching (cached thereafter)
• Bias: May reflect publication bias in biomedical literature and structural prediction limitations
• Static Data: Represents knowledge as of BioGRID 4.4.246 (June 2025) and doesn't include more recent discoveries
• Structure Quality: AlphaFold predictions vary in confidence; low-confidence regions may affect structure-based predictions
• Processing Time: Initial dataset generation with protein enrichment requires significant computational time

Ethical Considerations

• Data derived from publicly available scientific literature and databases
• No personal or sensitive information included
• Should be used to advance scientific understanding of biological systems
• Results should be validated experimentally before clinical or therapeutic applications

Additional Information

Licensing

The dataset inherits licensing from source databases:

• BioGRID: Free for academic and non-commercial use
• UniProt: Creative Commons Attribution (CC BY 4.0) License
• AlphaFold: Creative Commons Attribution (CC BY 4.0) License

Commercial users should review individual database terms of use.

Dataset Files

The dataset generation produces multiple output files:

• processed_dataset.json: Main conversational dataset
• processed_dataset_protein_data.json: Enriched protein information for reference
• protein_cache/: Directory containing cached API responses for efficient reprocessing

Citation

@misc{biogrid-conv-2025,
  title={BioGRID-Conv: A Conversational Dataset for Protein Interaction Network Prediction with Sequence and Structural Context},
  author={Proximile LLC},
  year={2025},
  url={https://huggingface.co/datasets/Proximile/BioGRID-Conv}
}

@article{biogrid2023,
  title={The BioGRID database: A comprehensive biomedical resource of curated protein, genetic, and chemical interactions},
  journal={Protein Science},
  year={2023},
  publisher={Wiley Online Library}
}

@article{uniprot2023,
  title={UniProt: the Universal Protein Knowledgebase in 2023},
  journal={Nucleic Acids Research},
  year={2023}
}

@article{alphafold2021,
  title={Highly accurate protein structure prediction with AlphaFold},
  journal={Nature},
  year={2021}
}

Technical Specifications

• Python Dependencies: pandas, numpy, networkx, requests, multiprocessing
• API Integration: UniProt REST API, AlphaFold API
• Caching System: Thread-safe file-based caching with fcntl locking
• Parallel Processing: Multiprocessing with configurable worker counts
• Memory Management: Batched processing with configurable limits

Dataset Version

Version 2.0 based on BioGRID 4.4.246 with integrated UniProt and AlphaFold data enrichment

Contact

For questions about this dataset, please refer to the HuggingFace dataset repository, visit proximile.llc, or consult the original database documentation.

About Proximile LLC

Proximile LLC provides secure, cost-effective, and private AI solutions tailored to small and medium-sized businesses. We specialize in:

• On-premise AI inference solutions that ensure unparalleled privacy
• Cost-effective hardware configurations including specialized AI hardware setups
• Secure Local AI applications including chatbots, RAG systems, and custom AI tools
• Specialized services for compliance & governance, knowledge management, and IT automation
• Bioinformatics AI Solutions for research institutions and biotechnology companies

Visit proximile.llc to learn more about our secure, local AI solutions for your business and research needs.

Funding

Original BioGRID data curation supported by the National Institutes of Health, Canadian Institutes of Health Research, and other funding bodies listed on the BioGRID website. UniProt supported by the National Institutes of Health and European Molecular Biology Laboratory. AlphaFold developed by DeepMind in partnership with EMBL-EBI. Dataset creation and curation by Proximile LLC.