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skip113
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bmc6M

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The schematic representation shows the conformational switch of a protein.There is common sequence motif in different membrane pore-forming proteins (PFTs). Pathogen utilizes this conformational switch strategy to evade the host cell membranes. The common motif would be useful for designing appropriate drug candidates, antibody/small molecule/peptide blocker to inhibit various pathogenic infections. The membrane-associated structures were obtained using OPM server (http://opm.phar.umich.edu/).
PMC7363679_42003_2020_1115_Fig2_HTML
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Follow-up images of the wound at two weeks postoperative for stitch removal
PMC10979719_cureus-0016-00000055137-i05
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Microstructure (a,b) and phase composition (c) of high-entropy CrMoNbWV alloy powder after MA and SPS (at temperatures of 1200, 1300, 1400 °C).
PMC7866258_materials-14-00621-g001
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
(a) Drug-induced Sweet Syndrome demonstrating tense vesicles and bullae with surrounding erythema over nose. (b) Tense inflammatory vesicles and bullae over central back. (c) Hemorrhagic bullae of distal finger pads and lateral fingers. (d) Erythema, edema, and focal bullous change overlying the cartilaginous portions of both ears. The non-cartilagenous lobes demonstrated no inflammatory changes. (e) Bullous lesions and erosions on the hard palate.
PMC2989706_AD2010-176749p001
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Flow chart describing the synthesis process for niobium oxide nanostructures by hydrothermal treatment.
PMC5480349_Beilstein_J_Nanotechnol-08-1205-g017
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Myocardial bridging (MB) demonstrated by invasive coronary angiography during diastole (a) and systole (b). There was a significant narrowing on the anterior descending coronary artery during systole (white arrows). The MB on the diagonal branch (red arrows) or any other branch was rare and not analyzed in this study. The length from the beginning to the end of the coronary artery narrowing was measured as MB length (c)
PMC8375129_12872_2021_2204_Fig1_HTML
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Subcutaneous morphine-induced MMP-9 increase is enriched in mu opioid receptor (MOR)-expressing DRG neurons. (A, B) Double staining showing colocalization of MMP-9 and MOR in DRG neurons of saline (A) and morphine (B) treated mice (s.c., 10 mg/kg). Scale, 50 μm. (C, D) Percentage of MMP-9+ (C) and MOR + (D) neurons in DRGs 2 h after saline and morphine injection. (E) Percentage of MMP-9 and MOR double-positive neurons in DRGs 2 h after saline and morphine injection. (F) Percentage of MMP-9-positive neurons expressing MOR and percentage of MOR-positive neurons expressing MMP-9 in DRGs 2 h after saline and morphine injection. S, saline; M, morphine. *P < 0.05, compared to saline, Student's t-test, n = 4 mice.
PMC3353172_1744-8069-8-19-3
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
a-c) Average fluorescence intensities of nuclear and cytoplasmic areas of cells seeded on substrates of 1.5 or 30 kPa stiffness and immunostained for importin α3 (imp α3) importin α1 (imp α1), and importin β1 (imp β1). N= 90 cells from 3 independent experiments. The effect of substrate stiffness tested significant for importin α3 (p=7.2e-8) and importin α1 (p=1.7e-5), but not for importin β1 (p=0.4971). p-values from Two-way ANOVA d-e) Corresponding example images showing the nucleus (Hoechst) and the distribution of the different importins. f) Corresponding quantification of nuclear to cytoplasmic ratio of importin localization. N= 91,98, 91, 98, 90, 90 cells (from left to right) from 3 independent experiments. p-values from independent two-tailed Mann-Whitney tests. g) N/C ratios of L_NLS-41 kDa or BFP constructs in cells seeded on 1.5 kPa gels before, during, and after nuclear deformation with AFM. h) L_NLS-41 kDa ratios normalized by BFP ratios, from panel g) paired measures. i,j) from g, corresponding paired dot plots of the time points right before and after force application. k) from g, corresponding % change in N/C ratios right after force application for both constructs. In g,h,i,j,k N= 15 cells from 3 independent experiments, p-values were calculated with a two-tailed paired t-test. l) N/C ratios of H_NLS-27 kDa construct in cells seeded on 1.5 kPa gels before, during, and after nuclear deformation with AFM. m) from l, corresponding paired dot plots of the time points right before and after force application. In l, m, N= 15 cells from 3 independent experiments. p-values were calculated with a two-tailed paired t-test. n) Corresponding images of constructs before and during force application, dotted line marks nucleus outline. o) N/C ratios of the L_NLS-41 kDa construct in cells co-transfected with DN-KASH and seeded on 1.5 or 30 kPa gels before, during, and after nuclear deformation with AFM. Data are mean ±SEM. p,q)from o, corresponding paired dot plots of the time points right before and after force application. In o,p,q, N= 15 cells from 3 independent experiments. p-values were calculated with a two-tailed paired t-test, traces of all cells are shown in Extended Data Fig. 8. r) Corresponding images of constructs before and during force application, dotted line marks nucleus outline. Scale bars, 20 μm. Note: in AFM experiments, non-mechanosensitive constructs (BFP and H_NLS) still show a small increase with force, likely due to lensing effects caused by changes in cell shape during indentation. This increase (~6% for BFP, ~2% for H_NLS) is much smaller than that of the mechanosensitive construct (L_NLS 41 kDa, ~14%), see panel k. Panel h in fact shows the response of the L_NLS construct after factoring out the response of BFP. Data are mean ±SEM in all panels. Source numerical data are available in source data.
PMC7614780_EMS179322-f009
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
(a) Vibratome sections of primary mouse RPE cells Efemp1WT/WT:C5+/+, Efemp1R345W/R345W:C5+/+, and Efemp1R345W/R345W:C5-/- cultured on transwells for two weeks and immunostained with antibodies for EFEMP1, C3/C3b/iC3b/C3d and C3dg, MAC. Note positive C3 staining at the bottom of the insert in Efemp1R345W/R345W:C5+/+ cultures and throughout the insert pores in Efemp1R345W/R345W:C5-/- cultures. This is due to the orientation of the sections. (b) Flat mounts of the bottom side of the inserts were immunostained with antibodies for ELN, TIMP-3, EFEMP1, CFH. EFEMP1 and CFH colocalize in the deposits. Scale bars a-i: 25 μm, j-aa: 100 μm.
PMC8128922_41598_2021_89978_Fig4_HTML
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Block diagram of signal conditioning circuit.
PMC6164658_sensors-18-02911-g004
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Aβ affects the nuclear localization of HLH-30 by activating mTOR. (A) mRNA expression of genes related to mTOR (strains GM101 and CL2122). (B) Representative Western blot showing phospho-RSKS-1 and total RSKS-1 in the CL2122 and GMC101 strains, three biological replicates. (C) Quantified Western blot gel intensities, as determined by ImageJ software. (D) GFP fluorescence images of HLH-30 entering the nucleus (n = 10 for each group, repeated three times). The dose of rapamycin was 100 μM. (E) Fluorescence signal ratio quantification. RPM, rapamycin. The HLH-30::GFP accumulation in JIN1821 was defined as 100% for quantitative comparisons. (F) Representative Western blot showing the content of HLH-30::GFP in the nucleus. Each experiment was repeated three times. (G) Quantified Western blot gel intensities, as determined by ImageJ software. **p < 0.01; ***p < 0.001. Detailed genotypic descriptions of strains CL2122, GMC101, and JIN1821 are listed in Supplementary Material.
PMC11392864_fphar-15-1433030-g001
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Anti-influenza effects of lycorine on the survival rate and lung index, pathological damage, and viral titers in lungs of mice. (A) In vivo schedule. The BALB/c mice (n = 11) underwent i.p. injection with 5 mg kg−1 day−1 of lycorine at 24 h before infection with 2LD50 virus and administered for 16 consecutive days. The mice from each group were randomly selected and euthanized on day 5 or 14. (B) Lycorine treatment increased the survival rate of the H5N1-infected mice. (C) Lycorine attenuated the lung indices, which were stimulated by H5N1 infection. Lung index = lung weight (g)/body weight (g) × 100. (D) Representative H&E staining images of lung and trachea tissues are shown as 400× magnification. (E) Viral titers in lung tissues of mice as determined by TCID50 assay. Data are mean ± s.d. (n = 5). *p < 0.05; **p < 0.01; ***p < 0.001.
PMC9343726_fmicb-13-862205-g005
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Sirt1‐immunoreaction was increased in Bmal1‐/‐ mice(A) Representative photomicrographs of Sirt1‐immunoreaction in DG (B) Quantification of Sirt1 immunoreaction (Ir). Sirt1‐Ir was significantly higher in Bmal1‐/‐. Values are shown as mean + SEM, **: P <0.01, scale bar = 50μm.
PMC4505169_aging-07-0435-g007
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
T therapy reduced adipogenic machinery and enhanced Myogenic gene machinery. T activate the MSCs via PAX7 to promote the commitment of MSCs to the myogenic lineage and subsequently activate MYF5 a key transcription factor for myogenic stem cells commitment to myoblast lineage. This in turn might increase the expression of its downstream effector myoblast determination protein 1 (MYOD1), a transcription factor promoting myoblast proliferation and FOLLISTATIN leading to myotube formation. On the other hand, Estradiol formed from T by the action of CYP19A1 activates the formation of PRDM16, which not only acts as a bi-directional switch between adipogenesis and myogenesis but also causes thermic browning which along with unknown downstream targets might enhance myogenesis. Thus, adipogenic gene machinery of PPARγ, and ADIPISIN are decreased significantly leading to reduction in fat mass by T therapy.
PMC11402695_fendo-15-1426175-g005
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Schematic representation of the heart with CS constraint references displayed. The blue and red color indicate poorly oxygenated and well‐oxygenated blood flow, respectively. The selected constraints are underlined. Abbreviations: IVC, inferior vena cava; SVC, superior vena cava. The base of the heart is defined as the union of the right atrium and ascending aorta. The anatomical definition is given in Supplementary information IX B.
PMC10092491_MP-50-397-g001
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Experimental optimization of the printing parameters for achieving uniform and long lines with sub-100 μm width comprising highly viscous Ag nanoinks.
PMC6266122_materials-11-02142-g003
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Chromosome staining by click raction.(a) Schematic of the click reaction for staining chromosomal DNA at the individual chromosome level. EdU was introduced into chromosomal DNA as a labeling tag for a click reaction. Alexa488-azide (green) or Alexa594-azide (red) reacted with EdU to stain the individual chromosome. (b) Chromosomes were stained with Alexa488-azide (green) or Alexa594-azide (red). The inset panel is at higher magnification. Observed by fluorescence microscopy.
PMC5020420_srep33217-f1
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Surface location of nerve block.
PMC8709754_JHE2021-7245566p006
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Transcription of centromeric repeats in response to genotoxic stress requires a wildtype p53 context.(A) Transcription of minor satellite repeats in WT (NIH3T3; p53+/+) or null (p53−/−) p53 contexts following ETOP treatment at the indicated time points, was analyzed as in Fig. 4A. *p < 0.05, **p < 0.01, ***p < 0.001 (unpaired t-test). (B) Enrichment of endogenous p53 at the p53 responsive element found at p21CIP promoter (p21-p53RE) or at minor satellite repeats in NIH/3T3 cells after a 4 hr treatment with ETOP, Nutlin-3 (N3) or both (N3 + E), analyzed by ChIP-qPCR. Data are normalized to the input then to the control condition. *p < 0.05, **p < 0.01 (unpaired t-test). (C) CENP-A localization in p53−/− fibroblasts treated with ETOP for 24 hr, analyzed as in Fig. 1A. Percentage of cells with delocalized CENP-A in p53−/− cells compared to p53+/+ cells are represented in the adjacent graph (n > 300 cells). Arrows point to the presence of micronuclei. ***p < 0.001 (chi-square test). (D) Percentage of cells with micronuclei in p53−/− cells compared to p53+/+ cells (n > 300 cells). ***p < 0.001 (chi-square test). CTRL: untreated cells; E: ETOP; N3: Nutlin-3a. Error bars represent S.E.M of at least three independent experiments. Images represent one focal plan. Scale bar, 10 μm.
PMC5301216_srep42520-f5
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Preoperative picture of the verrucous squamous cell carcinoma developing over a longstanding lesion of hypertrophic lichen planus.
PMC4006557_CRIDM2014-205638p002
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Chiral-Graphene-Metal geometry for hybrid Surface Waves.
PMC6303341_41598_2018_36241_Fig1_HTML
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
H&E staining for the PCL electrospun meshes implanted after 7 days. (A) Reconstruction of the full membrane; (B,C) Interior membrane sections; (D,E) End-limits of the meshes.
PMC9415937_polymers-14-03397-g003
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
AFM height and stiffness data for Rhodococcus wratislaviensis.AFM height (a–b) and stiffness (e) images of Rhodococcus wratislaviensis in their physiological (MM) medium. Height (c–d) and stiffness (f–g) profiles along dashed lines in figures (a) or (e), respectively, are plotted.
PMC3625152_ponep0061663pg001
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Chest X-ray imageScattered calcified lesions in the left lower lung.
PMC9904801_cureus-0015-00000033493-i01
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Architecture of YOLO-based object detection model.
PMC10065285_ponep0283801pg002
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Instrumental set-up for the (a) solid-liquid, (b) ultrasound, (c) microwave, and (d) infrared methods.
PMC6315536_antioxidants-07-00174-g001
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Harmful effects of different types aflatoxins contaminated edible oil.
PMC9573432_molecules-27-06141-g001
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Assessing the quality of RNA detection results.Graphic representations of the intensities of all detected spots relative to a chosen upper bound intensity and lower bound intensity. By comparing the results of different bounds, the user can determine an optimal minimum brightness for detecting gene transcripts. (A, B) Upper bound intensity of 0.07 and lower bound of 0.06. (C, D) Upper bound of 0.24 and lower bound of 0.23. B and D are the zoom-in of boxed subregion in A and C. The scale bar in A and C are 50 μm. (E) RNA detection by FISH-quant with the same data source in B or D. Detected dots with min_intensity = 200, quality score>60 (green circles). (F) Paired RNA detection are performed among 5 coverslips using SMART-Q or RNAscope. Paired t test. N = 12, P = 0.8030.
PMC7190163_ponep0228760pg002
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Transmission electron microscopy images of negatively stained Hafnia paralvei LY-23 cell infecting Hafnia phage Ca (A) and Hafnia phage Ca (B). Hafnia phage Ca particles possessed an icosahedral head with a diameter of 55 nm and a short tail with a length of 4.35–45.92 nm. The tail packaging tightness of Hafnia phage Ca is variable, presenting dot-like (blue arrow), bun-like (green arrow), cone-like with appendages (purple arrow), table tennis racket handle-like (yellow arrow), or ponytail-like (red arrow) shape. Each bun-like tail contains a visible tip sting and may be the tightened state of cone-like tails with appendages. Negative staining and electron microscopy observation, though, were redone four times in different laboratories and consistent results were obtained. The possibility cannot be ruled out that some differences might be artifacts. Cryo-EM observation could be a useful research direction for the phage in the future. The invading phages on the cell surface and the virus particles packaged in the cell are clearly visible (A). The cell wall in two places (see the enlarged photos at the bottom left for details) was invaginated due to the penetration of Hafnia phage Ca with the cone-like tails.
PMC8861465_fmicb-12-754331-g002
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Oncolytic virotherapy strategies for metastatic cancer treatmenta. Immunostimulatory factors (GM-CSF, IL-12) that are expressed in infected tumor cells recruit immune cells that induce tumor cell apoptosis. b. Anti-metastatic factors that are expressed in infected tumor cells block the metastasis pathway of the tumor cell and kill the tumor via their oncolytic capacity. c. Oncolytic virus infected stem cells replicate within the stem cells, which then migrate toward tumor lesions and release oncolytic viruses that infect tumor cells and induce oncolysis.
PMC5295462_oncotarget-07-58684-g002
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Device fabrication of the single-shot subpicosecond pulse laser spectrometer. This device consists of 3 important components: an ultrabroadband white-light femtosecond laser source for illumination, a wavelength-separated reflective diffraction grating device (600 lines/mm with blazed wavelength at 300 nm), and a CCD detector with 2,048 pixels for recording spectral intensity.
PMC10426273_researchp0210pfigp003
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Pterichis hirtziana Kolan., Szlach. & S. Nowak, sp. nov.(A) Floral bract. (B) Dorsal sepal. (C) Lateral sepal. (D) Petal. (E) Lip. Scale bars = 1 mm. Drawn by S. Nowak from van der Werff & Palacios 8956 (QCNE).
PMC7879944_peerj-09-10807-g023
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
(a) Scheme of the in situ fabrication of the Ru@Cu–TiO2/Cu. Characterization of Ti@Cu@Cu(OH)2 and Ru@Cu–TiO2/Cu: (b) HRSEM and (c) TEM images of TiO2@CuO@Cu(OH)2 NRs grown on a Cu current collector (CM). The solid lines in (c) outline the different surface layers. (d) SEM and (e) TEM images of the Ru@Cu–TiO2/Cu sample. (f) HRTEM image of the Ru@Cu–TiO2/Cu surface showing Ru nanocrystals along with Cu nanocrystals. (g–l) HAADF STEM image and corresponding EDS elemental maps for Ti, O, Cu, and Ru acquired on a small surface region of the Ru@Cu–TiO2/Cu sample. The EDS maps confirm that the elongated features visible in STEM image are Ru-rich, thus correspond to the Ru nanocrystals identified by HRTEM, viewed side-on. These Ru nanocrystals are placed on top of the Cu-rich nanocrystalline core covered with some Ti and O, corresponding to the TiO2 layer. In panel (l), the arrows indicate additional concentrated Cu species, found on surface of one of the Ru nanocrystals, and distinct from the subsurface Cu core nanocrystals. (m) HRSTEM image revealing crystalline features of Ru nanocrystals, and (n) fast Fourier transform from one of these nanocrystals. Additional high contrast features are observed on surfaces of Ru nanocrystals and on other surfaces corresponding to the TiO2 layer (arrowed). According to the corresponding (p) Cu EDS map, such features are enriched in Cu only. Closer view of these clusters containing Cu atoms without any crystalline arrangement is presented in Figure S16. These observations indicate that Ru nanocrystals, as well as other surfaces, are covered by very small/thin Cu clusters, as depicted in the model structure in (o).
PMC10557145_ja3c06726_0001
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
(a) Preparation of WS2@MWCNTs composite nanoparticles by in situ growth method, and (b) chemical schematic diagram of A-WS2@MWCNTs prepared by in situ grafting.
PMC8538699_materials-14-06195-g001
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
A flow diagram of study participants
PMC6239277_bsr-38-bsr20180464-g1
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Effects of C. albicans strains isolated from T2DM patients on the induction of insulin resistance.In both CD fed and HFD fed mice. a fasted blood glucose, b Body weight, c HbA1c, d HOMA-IR, e insulin level, f ITT on the 29th day, g OGTT, and h AOC on the 36th day of treatment, i representative images of H & E-stain, oil-red stain of liver tissue, and j representative images of H & E-stain of adipose tissue, Scale bars = 100 μm (n = 3 mice per group). Data are presented as the mean ± standard error of the mean (SEM); n = 9 mice per group. Statistical analysis was done using one-way ANOVA followed by the Tukey post hoc test. *P < 0.05; **P < 0.01 vs CD-WT group, and #P < 0.05; ##P < 0.01 vs HFD-WT group.
PMC9974954_42003_2023_4591_Fig3_HTML
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Immunofluorescent labelling of cell wall glycan epitopes in transverse sections from leaves and stems from 4 miscanthus genotypes. Immunolabeling studies were preceded by microscopic inspection of sections stained with toluidine blue to characterise their histological complexity. Monoclonal antibodies (mAbs) used in the study are directed at xyloglucan, MLG, xylan and pectin epitopes. More information on these and other mAbs is provided in Table 1 and in Additional file 1. A base treatment (BT) with 0.1 M KOH, which removed ester-linked substituents from the cell wall, was used together with CCRC-M144 and CCRC-M38. The non-base-treated control of CCRC-M155, as well as the complete immunolabeling study (total of 22 glycan-directed mAbs and 8 genotypes), is available in Additional file 2. ae, abaxial surface epidermis; bs, bundle sheath; on, organelle; gt, parenchymatous ground tissue; is, intercellular space; mf, mesophyll cells; mx, metaxylem; ph, phloem; px, protoxylem; sf, sclerenchyma fibres; st, stomatal complex; xp, xylem parenchyma. Scale bars: 100 µm
PMC6463665_13068_2019_1426_Fig4_HTML
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Self-assembly of branched nanocrystals via depletion interactions: (a) Non-assembled CdSe/CdS octapods. (b) Self-assembled structure induced by the addition of oleic acid. Republished with permission of the Royal Society of Chemistry, from [81]; Permission conveyed through Copyright Clearance Center.
PMC7517482_entropy-22-00877-g006
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Nucleic acid nanoparticles siAKR1C3@PPA complex targeted inhibits proliferation of CRPC cells. The NH2 group of PAMAM was covalently bound to the -NHS group of PEG. The sulfhydryl group of Apt-PSMA can continue to react with MAL of PEG.PAMAM and Aptamer were respectively attached to dual-function PEG via stable covalent bonds, resulting in the PPA that was still positively charged and can self-assemble with siRNA.Aptamer-PSMA acts as a target to guide siAKR1C3@PPA into PSMA-positive prostate cancer cells and specifically down regulate AKR1C3. Regulating Cell Cycle-related protein expression and inhibiting cell proliferation by decreasing AKR1C3 protein expression. Black arrow indicates negative regulation.
PMC9800608_fonc-12-1069033-g008
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
CRLM has significant amounts of microbiota, which promotes disease progression.A Overview of the fraction of microbiota from the tumor samples by 16 s rDNA sequencing. Patients are grouped into separate plots based on sample location and with or without liver metastasis (CRC-C, CRLM-C, and CRLM-L). Each color represents a kind of microbiota, and the length of each color represents the abundance of the microbiota in each kind of tumor sample in general. B The numbers of microbiota from the tumors (n = 20) in CRC-C, CRLM-C, and CRLM-L were collected and detected by qPCR. Error bars indicate SEM. Statistics were determined using a t-test with significance indicated (ns, not significant. **p < 0.01). C Representative whole-body bioluminescence images (up) of mice orthotopically xenografted after intravenous injection with MC38-luc+ cells and representative images of liver metastasis (down) from control, antibiotics-treated group (Abx) and E.coli-treated group mice (n = 10). D Representative co-immunofluorescence images of CD206(M2 marker) and DAPI (nuclear counterstain) in tumors from mice in (C). Scale bars, 100 μm. Objective, 10x. E Representative immunofluorescence images of mCherry(E.coli marker) in para tumor (PT) and liver tumor (T) from mice gavaged with mCherry-E.coli at day 21. Scale bars, 100 μm. Objective, 5x. F Representative co-immunofluorescence images of staining for mCherry(E.coli marker), CD206(M2 marker), Foxp3(Treg marker), and DAPI(nuclear counterstain) in tumors from mice in liver metastasis from control, low dose and high dose E.coli-treated group mice (n = 10) at day 21. Scale bars, 100 μm Objective, 10x and 5x. G Representative co-immunofluorescence images of staining for CD206(M2 marker) and DAPI (nuclear counterstain) liver metastasis from control, antibiotics-treated, and E.coli(iv) injected group mice (n = 10) at day 21. Scale bars, 100 μm. Objective, 10x. H Representative co-immunofluorescence images of staining for iNOS(M1 marker) and DAPI(nuclear counterstain) liver metastasis from control, antibiotics-treated, and E.coli(iv) injected group mice (n = 10) at day 21. Scale bars, 100 μm. Objective, 10x.
PMC11281901_41388_2024_3080_Fig1_HTML
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Cell properties during simulations and representative outputs. Four different properties of nodes and cells are indicated by colour mapping. All panels depict different features for the same simulated P3D SAM during a single time step. (a) Boundary nodes are shown in blue. Once the direction for FBoundary is determined as in §4.1, each boundary node is pulled in that direction with magnitude FBoundary/(num Boundary Nodes). All non-boundary wall-nodes are white, and cytoplasm nodes are not rendered. (b) Cells expanding out-of-plane (white) are chosen stochastically at simulation initiation and at the end of every cell cycle (details in §3.5). All other cells expand in-plane (red). (c) Cell growth directions are shown. Cells whose nodes are green (red) are preferentially expanding anticlinally (periclinally). Cells preferentially expanding out-of-plane or out-of-boundary grow with uniform mechanical properties along their wall (white). (d) Cell progress CPi (electronic supplementary material, section S1B) is shown for each cell. Cells with smaller CPi have recently finished a cell cycle, whereas cells with larger CPi are about to finish a cell cycle. (e) Visualization of all wall nodes in representative model simulations of both 2D and P3D simulations are visualized at t=40 h. Cell lines that were in L1 and L2 at time t=0 are shown with green wall nodes; all others are shown in white.
PMC10244971_rsif20230173f03
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Generation of mutations in Drosophila Atg9.(A) Schematic view of Atg9Gal4KO and Atg9d51mutations relative to the Atg9 transcripts. For the Atg9Gal4KO mutation, the complete Atg9 open reading frame was replaced with a Gal4 knock-in cassette. For Atg9d51 mutation, the 52–102 bp after the Atg9 start codon was replaced with the attPX-3-frameStop-floxed 3xP3-RFP cassette. (B) RT-PCR analysis of Atg9 mRNA expression level in control, mutant and Atg9 genomic rescue adult flies. Atg9 mRNA levels were undetectable in the Atg9 mutant. (C) Western blots show the endogenous Atg9 protein in control and Atg9 genomic rescue flies but fail to detect the protein in mutants. (D) LysoTracker Green staining reveals that starvation-induced autophagy is strongly reduced in Atg9 mutant fat bodies, compared with controls. Scale bar: 5 μm. (E) Quantification of data shown in (D). n ≥ 10, data are mean ±s.e.m. *p<0.05, **p<0.01. ns, not statistically significant. (F) Western blots show markedly increased Ref(2)P and ubiquitinated protein levels in Atg9 mutants. (G) Immunostaining of Drosophila thoracic muscles with anti-Ub (FK2) and anti-Ref(2)p antibodies showed an accumulation and colocalization of polyubiquitin protein aggregates and Ref(2)p (arrowheads) in Atg9 mutant flies. Scale bar: 10 μm. Df refers to Df(2R)Exel7142, which removes Atg9 and flanking genes.10.7554/eLife.29338.004Figure 1—source data 1.Quantification of lysotracker dots.
PMC5690286_elife-29338-fig1
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Experimental evolution design. V. cholerae A1552 and MO10 strains were grown overnight in LB broth. Genomic DNA from both strains was extracted and sequenced by Pac Bio. After dilution, the bacteria were grown to an optical density at 600 nm (OD600 nm) of 0.3 (ODay). Aliquots were spotted in technical triplicates on soft LB-agar supplemented or not with PmB. After incubation, samples of the different strains were taken from the edge of different protuberances of the flower-like patterns and streaked on LB agar. A colony from each of the different WT strains and their putative variants were grown overnight in LB broth and conserved at –80°C. The motility of the WT strains and variants was compared on soft agar plates supplemented with PmB. Motility diameters of the variants were measured and compared to those of their respective WT strains. The genomic DNA of selected hypermotile variants was extracted and sequenced by MiSeq.
PMC9454949_fmicb-13-932165-g002
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Patient with type 1 Brugada pattern, before and after presentation.
PMC8315878_CRP2021-5541385p001
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Proposed model of STING-dependent regulation of microglial function in genome instability. Persistent DNA damage, such as that observed in ATM deficiency, leads to formation of micronuclei in proliferating microglia. Activation of the cGAS-STING pathway culminates in IRF3 activation and production of type I interferons and chemokines, including CCL5 and CXCL10. Secreted inflammatory mediators likely mediate a transition from homeostatic functions of microglia to chronic inflammation and chemotaxis to reactive microglia via autocrine and/or paracrine signalling, promoting chronic inflammation and chemotaxis. Blockade of STING rescues this process and has potential to alleviate perturbations to CNS homeostasis in genome instability.
PMC10853792_gkad1184fig7
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Relative deficits in passive shoulder flexion (A) and abduction (B) for latissimus dorsi donor site[24], pectoralis major donor site[45], and spinal accessory nerve (CNXI) sacrifice[43]. CNXI: spinal accessory nerve.
PMC8323836_nihms-1683328-f0006
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
SEM images; (a) LPR; (b) 10% PBS addition; (c) 20% PBS addition; (d) 30% PBS addition; and (e) 40% PBS addition.
PMC10094893_ijms-24-06418-g007
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Ktrans and kGad (A) and test-retest kw (B) maps from subjects with (top row) and without (bottom row) WMH. (C) FLAIR, Ktrans, kGad, and kw maps (first visit) in normalized MNI space from the same subject as shown in the top row. Ktrans, kGad, and kw maps were overlaid on the FLAIR image. One WMH lesion was indicated by a red arrow and a dashed red circle and WMH penumbra was indicated by an orange circle on each image.
PMC7733970_fnins-14-571480-g0008
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Coordination environment around the Tl atom in the title compound, showing an empty space on one side of the atom Tl1. Displacement ellipsoids are drawn at the 50% probability level. [Symmetry codes: (i) 1/2-x, -1/2+y, 3/2-z; (ii) -1+x, y, z; (iii) -x, -y, 1-z.]
PMC2967867_e-65-00m17-fig1
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Geometric representation of Case 1.
PMC10791672_41598_2024_51706_Fig1_HTML
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
(A, B) Biofilm inhibition percentage of Se-BiO-CuO MMNPs against E. coli biofilm-forming bacterial strain and (C, D) anti-biofilm images of untreated and treated E. coli cells (p < 0.05).
PMC11037251_fcimb-14-1301351-g011
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Diagnostic nasal endoscopy showed a greyish mass filling the whole of the right nasal
PMC7007993_ijo-32-053-g001
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
The experimental design of the creation of a Leaky Gut Chip for validating probiotic effects. (a) The workflow illustrating the growth of intestinal epithelial Caco-2 cells in a Gut Chip that employs a physiological oxygen gradient and host-probiotics co-cultures with either LGG or VSL#3 cells. The “−O2” and “+O2” mean the absence and the presence of oxygen in the culture medium, respectively. Bidirectional grey arrows in the middle schematic show cyclic mechanical deformations in a Gut Chip. V, cyclic vacuum suctions. A dashed line in the right schematic shows the location of a porous basement membrane in a Gut Chip. Blue and pink arrows display the direction of the culture medium in a Gut Chip. (b) An experimental timeline to induce a leaky epithelial barrier and cellular inflammation followed by probiotic amelioration. Epithelial Caco-2 cells are cultured in a Gut Chip under flow and motions for 5–6 days at a high serum condition (20% (v/v) FBS), then switched to a low-serum (5% FBS) culture medium that contains a cytokine cocktail (TNF-α and IL-1β) for 3 days. Probiotic co-cultures were performed after epithelial cells were stabilized to an oxygen gradient and an antibiotic-free condition. During the probiotic co-cultures, cytokine treatment was discontinued. AOI, anoxic–oxic interface; Abx, antibiotics. “D” indicates the day of cultures. We set D0 as the first day of cytokine treatment. D-1 and D-6 denote the pre-culture of Caco-2 cells in a Gut Chip on 1 and 6 days before the cytokine treatment, respectively. The pink and green boxes indicate the period of a cytokine challenge and a probiotic co-culture, respectively.
PMC9805460_41598_2022_27300_Fig1_HTML
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
A axial CT scan showing a pantaloon urinary bladder herniation (red arrow) with a calculus (blue arrow).
PMC9729010_cureus-0014-00000031208-i01
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Effect of isovitexin treatment on S. aureus-induced pneumonia in mice.(A) Effect of isovitexin treatment on the survival of mice (n = 10) infected with a lethal dose of S. aureus. Mock vs. isovitexin, **p < 0.01; log-rank test. (B) Effect of isovitexin treatment (100 mg/kg) on the bacterial load in lungs of mice (n = 5). **p < 0.01, ***p < 0.001; Mann‒Whitney test, twotailed. The horizontal bars represent the means. (C) Histopathology of lungs (H&E-stained tissues) of mice treated or not treated with isovitexin (100 mg/kg). The animal data were obtained from two separate experiments.
PMC9668100_jmb-32-10-1284-f5
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Resistant and susceptible strains of E. coli on agarose pads containing an inhibitory concentration of tetracycline.(A) Phase contrast images and associated fluorescence overlays. RFP expressing cells contain a tetracycline resistance gene and GFP expressing cells do not. The magenta and green cell outlines in the fluorescence overlay represent the resistant and susceptible cells, respectively. Region of interest boxes show the areas represented in (B). (B) Representative examples of antibiotic resistant and susceptible cells tracked over time. (C) RFP and GFP fluorescence tracked for individual cells over time. (D) GFP fluorescence versus RFP fluorescence for single cells plotted against growth rate. Fluorescence values are the averages over all the frames for that cell. For growth rate calculations, only cells that were present at t = 150 min were tracked, which is a time point mid-to-late in the movie. The analysis omits those cells that enter the field of view after t = 150 min since the growth rates become noisier with less data. Three resistant cell outliers with growth rates of ~1.4 1/hr are omitted from this view.
PMC8797229_pcbip1009797pg002
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Bioactivity-guided molecular network using LC-MS/MS and bioactivity data. Bioactive mass peaks are highlighted by size, and the most significant are presented as octagonal shapes (p < 0.01 and correlation > 0.95). The color within the nodes corresponds to their relative quantity in the analyzed fractions: green, active fraction #192; yellow, non-active fraction #237; orange, non-active fraction #207; all from Synechoccocales.
PMC8003170_marinedrugs-19-00161-g004
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Distinguishing VSV inclusion bodies from individual VSV RNPs in living cells.A549 cells were infected with VSV-PeGFP (MOI = 3) and imaged by fluorescence microscopy at 100 frames/s between 4 and 5.5 hpi, a single frame of which is shown (A). (B) The same image as panel A after increasing the minimum fluorescence intensity threshold above that of individual VSV RNPs. (C) Magnified image of panel A. (D) Magnified image of panel B. Arrow indicates example of VSV inclusion body chosen for analysis. (E) Approximate diameters of fluorescently labeled VSV inclusion bodies (n = 97) in control cells and in cells treated with 0.25 μM latrunculin A (n = 113) were determined by quantifying pixel fluorescence intensities using ImageJ as described in Materials and Methods. The size distributions of the two inclusion body populations were not significantly different (2.03 ± 0.59 μm in controls versus 1.96 ± 0.35 in treated, p > 0.05 by Student’s t-test).
PMC10939199_ponep0290672pg001
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Effect of nutritional interventions in plaque accumulation in the aorta. The effect of different food and extracts in reducing plaque are listed for the aortic arch/sinus, the brachiocephalic artery, the descending aorta, and the entire aorta. Foods that showed no effect and the ones that increased plaque are also listed. Studies using both males (M) and females (F) are also identified.
PMC7400924_nutrients-12-02069-g003
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Neutralizing AREG antibodies revert osteoclast differentiation induced by NSCLC-exosomes. (a) TRAP staining of RAW 264.7 cells incubated with: CRL-2868 exosomes, Rec-AREG, CRL-2868 exosomes plus AREG neutralizing antibodies, for 6 days, stained for TRAP and compared with untreated cells (Ctrl). Scale bar 10 µm. (b) Evaluation by real Time PCR analysis of mRNA expression of TRAP and MMP9 in RAW 264.7 cells treated, for 6 days, with: CRL-2868 exosomes, Rec-AREG, AREG neutralizing antibodies, RANKL, CRL-2868 exosomes plus AREG neutralizing antibodies. (c) MMP9 protein levels assessed by ELISA, in RAW 264.7 cells treated, for 6 days, with: CRL-2868 exosomes, Rec-AREG, AREG neutralizing antibodies, RANKL, CRL-2868 exosomes plus AREG neutralizing antibodies. Values are the mean ± SD of three independent experiments *p ≤ 0.05, **p ≤ 0.01.
PMC5466625_41598_2017_3460_Fig7_HTML
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
In general, it is impossible for all the cosines of the angular lengths of all three sides of the spherical triangle △(X=0,X=1,Y=0) to be rational, and the internal angle γ to be a rational multiple of π. That is, the finiteness conditions for Hilbert states cannot be satisfied for a counterfactual measurement X = 1, Y = 0 on a particular particle pair, when it is satisfied for a realizable measurement X = 0, Y = 0 on the same particle pair. Because of this, statistical independence and factorization are violated in the invariant set model. However, precisely because the counterfactual state violates the finiteness conditions and therefore does not correspond to a state of physical reality on IU, the invariant set model does satisfy ‘Statistical Independence on IU’ and ‘Factorization on IU’. Hence whether invariant set theory is local or non-local depends critically on whether locality should be expressible entirely in terms of changes to quantities defined in space–time, or should have unrestricted access to counterfactual states in potentially non-iontic parts of state space.
PMC7209156_rspa20190350-g6
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Molecular structure of compound 14 showing 50% displacement ellipsoids.
PMC10666145_ao3c06461_0008
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
AFM imaging of DNA loop, GTF, nucleosome and HMGA1 positioning on the IL2RA gene promoter.(A) AFM imaging in liquid of DNA loop positioning along the 1290 bp IL2RA fragment (upper right panel) and the 898 bp IL2RA fragment (lower right panel) and statistical analysis (left panel) of their positioning given by the loop middle point (green vertical bars) from N = 21 AFM images. (B) AFM imaging of TBP-TFIIB positioning along the 563 bp IL2RA fragment (right panels) and statistical analysis (left panel) of their positioning (orange vertical bars) from N = 51 AFM images. (C) AFM imaging of mononucleosome positioning along the 898 bp IL2RA fragment (right panels) and statistical analysis (left panel) of dyad positioning (blue vertical bars) from N = 100 AFM images. (D) AFM imaging of HMGA1 positioning along the 898 bp IL2RA fragment (right panels) and statistical analysis (left panel) of dyad positioning (blue vertical bars) from N = 73 molecules.
PMC3076448_ponep0018811pg003
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Test of the impact of genomic events on cancer task specialization.a) Alignment of vectors with the Pareto front in degree (0°: perfectly aligned, 90°: completely orthogonal) and (b) length of the vector. P values correspond to two-sided Wilcoxon tests between observed and shuffled vector distributions. Source data
PMC10101853_41588_2023_1321_Fig14_ESM
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Magnetic resonance imaging (MRI) of both the ankles. Arrows show cystic lesions in both the tibia and talus. Degenerative change is also seen in right cuneiform.
PMC4202255_CRIM2014-931278p002
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Crystal orientation distribution diagrams of (a) AA2219 alloy and (b) 5 wt.% nanosized TiB2/AA2219 composite.
PMC7579223_materials-13-04250-g006
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
(a) Gastroscopy showed a round tumor at the anterior wall of the gastric body. (b) The resected tumor was 2 cm in diameter.
PMC2946604_CRM2010-348761p001
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Power-knowledge grid of identified stakeholder groups. The stakeholder division over the power axis is based on the TSW organization within the UNFPA EmONC development approach and further explained/substantiated by the arrows within the figure. The level of relevant knowledge attributed to each stakeholder group is based on the survey results (Supplementary Table 2).
PMC9889992_fpubh-10-1051522-g0003
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Traffic Flow Prediction Model Based on TCN Attention.
PMC10422360_sensors-23-06683-g001
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Nerve regeneration assessments of the prevascularized TENGs after nerve bridging. At 12 w after nerve defect bridging, the prevascularization for TENGs reflected a stronger ability to repair nerve defects in regenerated nerve tissue structures, electrical signal conductions, and reinnervation of target organs. (A) A schematic illustration of the prevascularized TENGs and the functional evaluations at 12 w after nerve bridging. (B) The transmission electron microscope pictures of distal regenerated nerves, and histograms of the thickness of myelin sheaths and the number of myelin sheath layers (n ​= ​5). Scale bar, 5 ​μm, 0.25 ​μm, and 0.05 ​μm respectively. ∗, each group vs. control group. ∗p ​< ​0.05; ∗∗p ​< ​0.01. (C) The electrophysiological images in waveforms and the histograms of CMAPs amplitudes recorded at the proximal end of TENGs (n ​= ​5). “a” meant the records at distal ends. “b” meant the records at proximal ends. ∗, each group vs. control group. ∗p ​< ​0.05; ∗∗p ​< ​0.01. (D) The gross view of dissected gastrocnemius and anterior tibialis on the surgical and contralateral sides. The histograms of the muscle wet weight ratios (%) (n ​= ​5). Scale bar, 1000 ​μm ∗, each group vs. control group. ∗p ​< ​0.05; ∗∗∗p ​< ​0.001.
PMC10339252_gr8
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Procedure description for the k-th subject used in training and test datasets.
PMC11359939_sensors-24-05428-g002
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Perspective view of the title molecule with numbering of the atoms. Non H-atoms represented as displacement ellipsoids are plotted at the 30% probability level, while H atoms are shown as small spheres of arbitrary radius.
PMC3238971_e-67-o3321-fig1
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Knockdown of chemerin suppressed intimal hyperplasia of carotid arteries in vivo.(A) The increased mRNA and protein levels of chemerin induced by angioplasty in carotid arteries were significantly reduced by local perfusion of lentivirus (n = 3), while serum chemerin levels showed no significant changes after lentivirus perfusion (n = 6). * p<0.01. (B, C) Neointimal hyperplasia of carotid induced by angioplasty and the increased ratio of neointimal area to medial area were inhibited by knockdown of chemerin at day 14 post- angioplasty. * p<0.01, n = 6. (D, E) VSMCs proliferation in carotid arteries induced by angioplasty and the increased BrdU-positive VSMCs were inhibited by knockdown of chemerin at day 14 post-angioplasty. * p<0.01, n = 6.
PMC5085037_ponep0165305pg003
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Superoxide and antioxidant processing in the nucleus. Molecular oxygen and H2O2 may enter the nucleus through the nuclear pores. The Fe–S clusters of nuclear proteins are oxidized by superoxide (O2·−). The oxidation of protein [4Fe–4S] clusters by molecular O2 produces Fe (II) and O2·−. Superoxide may also be generated by RBOH enzymes in the nucleus. These could be delivered to the nuclear membrane by vesicle transport from the plasma membrane. The nuclear antioxidant system includes ascorbate (AsA), GSH catalase 2 (CAT2), ascorbate peroxidase (APX1), iron superoxide dismutase 1 (FSD1), and glutathione peroxidase 8 (GPX8). 2-OGDD, Fe2+/2-oxoglutarate-dependent dioxygenases.
PMC11317529_erae090_fig2
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
The risk factors for AA formation and the classification of aortic aneurysm and aortic dissection
PMC9898314_41392_2023_1325_Fig1_HTML
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
A) An illustration showing the impedimetric sensor attached to a plant leaf. B) Schematic illustration of the fabrication process of the MNs‐equipped electrodes: I) A master mold is fabricated using TPP laser lithography on a Silicon die. II) After development, the mold is coated with a 5 µm layer of Parylene C via chemical vapor deposition (CVD). III) Uncured PDMS (1:10, curing agent to monomer) is cast on top of the master mold and is cured at 90 °C for 45 min. IV) The SU‐8/polyimide varnish is cast onto the PDMS imprinted mold and is outgassed and appropriately cured. V) The mold is submerged in chloroform and is sonicated at a frequency of 45 kHz for 1 min. VI) The MNs are demolded and layers of 10 nm of titanium followed by 150 nm of gold are sputtered on the MNs. C) Image of polyimide (left) and SU‐8 (right) MNs after demolding. D) SEM images of polyimide MNs at two magnification levels.
PMC8373106_ADVS-8-2101261-g008
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
A gray-black mass compatible with bezoar was seen in the stomach via upper gastroendoscopy.
PMC11346831_medi-103-e39227-g002
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Crystal structure of the Y307F zHDAC10Δ–AAT complex.(a) The catalytic PDAC domain and the smaller, noncatalytic ΨDAC domain assemble with butterfly-like architecture. The unique 310-helix ηA2 (P23ACE) found in the L1 loop of HDAC10 orthologues is purple. Percentages indicate sequence identity/similarity between zebrafish and human HDAC10 domains. The inhibitor AAT (stick figure, C=yellow, N=blue) binds to the PDAC domain. (b) Superposition of the PDAC domain (blue) with the ΨDAC domain (green). Selected secondary structure elements are labelled: helix ηA2 is purple; helices αF, αG and the loop connecting helices αH1 and αH2 mediate domain–domain interactions; helices αB2 and αB3, as well as loops L1–L5, comprise and flank the active site of PDAC but are absent in ΨDAC (for clarity, only loops L2, L3 and L5 are labelled). Zn2+ is a blue sphere. (c) Stereo view image showing the superposition of zHDAC10 PDAC domain (blue), zHDAC6 CD1 (wheat, PDB 5EEF) and zHDAC6 CD2 (light blue, PDB 5EFH). The 310-helix ηA2 inserted in loop L1 (purple) is unique to zHDAC10 and serves to constrict the PDAC active site. (d) Stereo view image showing the simulated annealing omit map of AAT bound in the PDAC active site contoured at 3σ. Selected active site residues are indicated; hydrogen bonds are indicated by dashed red lines and metal coordination interactions are represented by solid black lines. Zn2+ is a blue sphere.
PMC5454378_ncomms15368-f3
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Example test case from the “seen” data data source (Dataset 1). The performance of the top algorithms #53 and #38 is shown in green and blue, respectively. Ground truth annotations are shown in red (a: axial view, b: coronal view).
PMC8183044_nihpp-rs571332v1-f0006
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Simple pendulum representation
PMC9649460_11229_2022_3949_Fig1_HTML
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Ablation efficiency of DDP/PLGA-30% Fe3O4 in excised bovine liver.(A) Thermal images of excised bovine liver containing 50 μl and 100 μl DDP/PLGA-30% Fe3O4 under AMF for 3 min, with excised bovine liver containing 100 μl DDP/PLGA as a control. (B) The corresponding time-temperature curve of the excised bovine liver exposed to AMF for 3 min. (C) The distance–temperature curve of the excised bovine liver containing 100 μl DDP/PLGA-30% Fe3O4 after 3 min exposure to AMF. (D) The corresponding ablation volume of the excised bovine liver containing 50 μl and 100 μl DDP/PLGA-30% Fe3O4 followed by exposure to AMF for 1 min, 3 min, and 5 min, respectively. (E) The corresponding macroscopic photos. (F) US images of excised bovine liver before and after injection with 100 μl DDP/PLGA-30% Fe3O4 followed by exposure to AMF for 3 min.
PMC5417648_ponep0177049pg004
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Electroencephalography of an absence seizure with eyelid myoclonia in Jeavons syndrome. (A) The absence seizure was triggered by eye closure at X. Note the paroxysm of generalized, fast polyspike activity (X–Y). This seizure was semiologically characterized by eyelid myoclonus, hyperextension of the neck, and unresponsiveness. (B) Interictal generalized polyspike-wave discharges during sleep recorded from the same patient.
PMC5622315_fneur-08-00499-g007
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
(a) Bacterial inhibition kinetics of Pd-Ag/rGO on E. coli K-12 in the presence of light (Blue line) and in the dark (Red line). (b) Bacterial inhibition performance of Pd-Ag/rGO on E. coli K-12 with light.
PMC7911859_biomolecules-11-00190-g009
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Figure illustrates the homogenization procedure for solid tissue specimens.
PMC11265891_tjb-48-03-203f2
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Mechanisms of anti-HIV activity by drug class
PMC5530928_40794_2016_42_Fig1_HTML
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Possible model of Ap functions in the consolidation and maintenance of LTM.(A) The Ap/Chi complex induces gene expression. Subsequently, proteins required for maintaining consolidated LTM are produced in MB α/β neurons. (B) In l-LNvs, Ap may inhibit GABA responses via Rdl in a Chi-independent manner. (C) Intercellular communication from l-LNvs to MB neurons plays a crucial role in forming stable LTM. Non-MB neurons may mediate intercellular communication between l-LNvs and MB neurons. Ap, Apterous; Chi, Chip; GABA, gamma-aminobutyric acid; l-LNv, large ventral–lateral clock neuron; LTM, long-term memory; MB, mushroom body.
PMC8641882_pbiop3001459pg010
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Microscopic examination of head of epididymis minced solution in rats stained with Eosin & Nigrosin stain: (a) normal sperm head and tail and (b) abnormal sperm head and tail, (c) abnormal sperm head and tail, (d) amorphous head, (e) abnormal head, (f) double head, (g) coiled tail, and (h) double tail.
PMC9363220_BMRI2022-1546734p006
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Geometrical comparison. (a) VDI 0 (b) VDI 27.
PMC8150364_materials-14-02497-g020
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
3D visual model of the renal collecting system, renal calculi, and grid patch. (A–C), Front, side, and back views of the 3D visual model of the renal collecting system (red), kidney calculi (green), and grid patch (gold). The figure was created with Mimics 19.0 (https://www.materialise.com).
PMC8055667_41598_2021_87972_Fig2_HTML
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
DNA damage response. DNA damage is caused by endogenous agent oxygen species (ROS) or exogenous agents such as UV light, ionizing radiation (IR) and chemotherapy agents. DNA damage response (DDR) is induced to deal with the lesions, including signal transduction, transcriptional regulation, cell-cycle checkpoints, induction of apoptosis, multiple DNA repair pathways as well as damage tolerance processes. DNA repair pathways include nuclear and mitochondrial DNA repair pathways. Direct repair, BER, MMR and recombinational repair (HR and NHEJ) are existence in both nuclear and mitochondrial repair systems. NER has been reported only appearance in nucleus, and the existence of TLS pathway in mitochondria is unknown. NDNA, nuclear DNA; MtDNA, mitochondrial DNA; BER, base excision repair; HR, homologous recombination repair; NHEJ, non-homologous end joining; MMR, mismatch repair; TLS, translesion synthesis; NER, nucleotide excision repair.
PMC7898236_fphar-11-629266-g001
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Biopsy specimen of Ewing sarcoma in left femur at diagnosis. Panel (A) shows CD3 staining. By IHC staining, (B) TIM-3 and (C) Galectin-9 are both strongly positive on non-tumor cells only and location of checkpoint receptor/ligand congregating at tumor border, (D) PD-1 and (E) PD-L1 are partially positive on non-tumor cells only, (F) LAG-3 is negative on both tumor and non-tumor cells, and (G) MHC class II is positive on tumor cells only.
PMC9351179_nihms-1818222-f0002
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Blueprint of information architecture and a data sharing model for open patient data 161 ecosystems.
PMC10456860_fpain-04-1208513-g001
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Capillaroscopic evaluation in BMS patients after laser application.
PMC7559391_dentistry-08-00099-g004
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Secretion of LpAsp and LpCht proteins. a Visualization of L. passim expressing GFP, LpAsp-GFP or LpCht-GFP fusion proteins using both visible differential interference contrast (DIC) and fluorescence (Fluorescence) microscopy. Merged images are also presented (Merge). Note that fluorescence signals for LpAsp-GFP and LpCht-GFP were enhanced through longer exposure times compared to GFP alone. Scale bar: 1 μm. b Schematic representation of LpAsp and LpCht proteins. The positions of amino acids encoding SP and molecular functional domain are shown together with those of potential N-glycosylation sites (Y). The total number of AAs in each protein is given at the right. The size of protein is not in scale. c Quantification of intracellular GFP, LpAsp-GFP, and LpCht-GFP via WB analysis of cell lysates, with WT L. passim used as a control. Extracellular protein levels were determined through IP of equal volumes of conditioned medium, followed by WB. Arrowheads indicate two cleaved LpCht-GFP proteins. SDS-PAGE gels stained with InstantBlue displaying the cell lysates and concentrated conditioned medium are presented at the bottom. Equal protein amounts were loaded for the WB or IP analysis. Molecular weights (kDa) of the protein markers are indicated on the left. AA, Amino acid; GFP, green fluorescent protein; IP, immunoprecipitation; LpAsp-GFP, L. passim aspartyl protease–GFP fusion protein; L. passim LpCht-GFP, chitinase-GFP fusion protein; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; SP, signal peptide; WB, western blot; WT, wild type
PMC10859015_13071_2024_6126_Fig2_HTML
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Flowchart of included children with clubfoot
PMC8161945_12891_2021_4323_Fig1_HTML
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Parkin promotes BRCA1 degradation after mitochondrial damage via the ubiquitin–proteasome pathway and is co-localized in nucleus. (a) MCF7 and MCF7-Parkin cells were treated with CCCP at the indicated concentrations for 24 h, and then BRCA1 protein level was assessed by Western blotting. (b) Protein levels of BRCA1 as assessed by Western blotting. MCF7 and MCF7-Parkin cells were treated with 10 μM CCCP for the indicated times. Representative images from seven independent experiments are shown. TOM20 and COX IV were assessed to verify the occurrence of mitophagy. Because the overexpression of Parkin accelerates mitophagy, the degradation of TOM20 and COX IV in MCF7-Parkin cells after CCCP treatment was faster than that in MCF7 cells. (c) Densitometry analysis of BRCA1 in (b). Each band intensity was standardized against β-actin, and relative band intensities were calculated. n = 7, bar = means ± S.E., *p < 0.05 versus MCF7 at the same time point. (d) MCF7-Parkin cells were separated into the cytoplasmic, nuclear, and mitochondrial fractions after treatment with 0 or 10 µM CCCP and 0 or 10 µM MG132 for 24 h. Each fraction was subjected to Western blotting. Lamin A/C, α-tubulin, and COX IV were used as markers of the nuclear, cytoplasmic, and mitochondrial fractions, respectively. (e) Subcellular localizations of BRCA1 and FLAG-Parkin in MCF7-Parkin cells were assessed by immunofluorescence. Cells were treated with a vehicle, 10 μM CCCP, or 10 µM CCCP + 10 μM MG132 for 5 h. The treatments are indicated on the left side, and the detected proteins are indicated at the top. In the merged panels, BRCA1, FLAG-Parkin, and the nuclei are shown in green, magenta, and blue, respectively. The small boxed images are enlarged in the right panels. Scale bar = 10 μm.
PMC8062582_41598_2021_87698_Fig2_HTML
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Saccular otoliths are not tethered correctly in the homozygous ext2 mutant ear at 5 dpf. (A–C”’) Live DIC images of embryos at 5 dpf, before (left-hand column) and after (right-hand column) tapping. In phenotypically wild-type sibling embryos (A,A’), saccular otoliths (green arrowheads) remain in place after tapping (in this example, one of the utricular otoliths in (A’) has become slightly displaced). In ext2 mutant embryos (B–D’), the saccular otolith in one or both ears becomes displaced (magenta arrowheads show new position after tapping). (A–C’) are dorsal views with anterior to the left; (C”,C”’) are lateral views of the embryo shown in (C,C’). Note also the swollen ear morphology in the ext2 mutants [(B,C), black arrowheads]. In all panels, anterior is to the left. Scale bar in (A), 100 μm (applies to all panels).
PMC9458858_fcell-10-959624-g005
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Cryolesion (diameter = 0.80 cm) at 1 minute and 30 seconds into the freeze cycle. Note the hyperechoic rim with posterior acoustic shadowing emanating from the tip of the probe.
PMC3350006_CRIMpANESTHESIOLOGY2011-691478p004
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
Flow chart of study population. Study population stratified on patients with and without their left ventricular ejection fraction measured
PMC2576460_1471-2261-8-28-1
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar
KCNC3R423H causes dominant electrophysiological and trafficking effects on KCNC3WT.(A) Representative currents evoked by a step from −70 mV to +70 mV in CHO cells expressing KCNC3WT-Clover or KCNC3R423H-Clover, and in those transfected with both constructs KCNC3WT-Clover:KCNC3R423H-mRuby2 in a 1:1 ratio. (B) Mean current densities recorded in CHO cells expressing either wild-type KCNC3WT-Clover (n = 7) or KCNC3R423H-Clover (n = 5) and in those expressing both KCNC3WT-Clover: KCNC3R423H-mRuby2 (1:1) constructs (n = 6). Current density was calculated by dividing the peak current evoked by a step from −70 to +70 mV by cell capacitance. Values are shown as mean±SEM, and significance was tested using a one-way ANOVA. (C) Current-voltage relations for cells in the three conditions shown in (A) and (B). Confocal fluorescence microscopy of cells expressing KCNC3WT-Clover (D) or KCNC3R423H-mRuby2 (G) individually, with no channel bleed-through (E,F). (H-S) Confocal fluorescence microscopy of cells co-expressing KCNC3WT-Clover and KCNC3R423H-mRuby2 at ratios of 1:1 to 6:1 (KCNC3WT:KCNC3R423H) showing co-localization and intracellular retention of both proteins, even at the highest concentration of KCNC3WT. The total amount of DNA used in the co-transfection experiments was kept constant across ratios by adding control plasmid pcDNA 3.1.
PMC5414954_ponep0173565pg005
hf://datasets/skip113/bmc6M@6e4197c66051f5bc6df5c97702ac633bc1669704/data/000001.tar