Popular Vote (popV) model for automated cell type annotation of single-cell RNA-seq data. We provide here pretrained models for plug-in use in your own analysis. Follow our tutorial to learn how to use the model for cell type annotation.

Model description

Tabula Sapiens is a benchmark, first-draft human cell atlas of over 1.1M cells from 28 organs of 24 normal human subjects. This work is the product of the Tabula Sapiens Consortium. Taking the organs from the same individual controls for genetic background, age, environment, and epigenetic effects, and allows detailed analysis and comparison of cell types that are shared between tissues.

Link to CELLxGENE: Link to the data in the CELLxGENE browser for interactive exploration of the data and download of the source data.

Training Code URL: Not provided by uploader.

Metrics

We provide here accuracies for each of the experts and the ensemble model. The validation set accuracies are computed on a 10% random subset of the data that was not used for training.

Cell Type N cells celltypist knn bbknn knn harmony knn on scvi onclass scanvi svm xgboost Consensus Prediction
basal cell 3375 0.86 0.80 0.88 0.73 0.00 0.68 0.87 0.86 0.93
bladder urothelial cell 2721 0.99 0.91 0.91 0.66 0.00 0.55 1.00 0.99 0.99
luminal epithelial cell of mammary gland 1512 0.99 0.18 0.97 0.67 0.00 0.60 0.99 0.98 1.00
enterocyte of epithelium proper of duodenum 1378 0.92 0.62 0.80 0.47 0.00 0.27 0.91 0.91 0.94
stratified squamous epithelial cell 1184 0.82 0.81 0.90 0.79 0.00 0.67 0.84 0.84 0.89
acinar cell of salivary gland 1178 0.94 0.85 0.90 0.62 0.00 0.38 0.96 0.95 0.97
pulmonary alveolar type 2 cell 1183 0.95 0.97 0.96 0.77 0.00 0.64 0.97 0.96 0.97
conjunctival epithelial cell 1136 0.88 0.75 0.82 0.48 0.00 0.10 0.95 0.92 0.94
luminal cell of prostate epithelium 1088 0.93 0.80 0.92 0.72 0.00 0.47 0.96 0.94 0.96
kidney epithelial cell 939 0.99 0.81 0.92 0.51 0.00 0.22 1.00 1.00 1.00
hepatocyte 739 1.00 1.00 0.97 0.64 0.00 0.33 1.00 1.00 1.00
enterocyte of epithelium proper of ileum 678 0.98 0.26 0.83 0.35 0.00 0.06 0.98 0.98 0.98
pancreatic acinar cell 530 0.93 0.67 0.94 0.59 0.00 0.19 0.98 0.97 0.97
enterocyte of epithelium of large intestine 475 0.81 0.38 0.56 0.41 0.00 0.04 0.82 0.87 0.83
corneal epithelial cell 422 0.92 0.00 0.94 0.71 0.00 0.60 0.96 0.95 0.97
duct epithelial cell 370 0.80 0.58 0.79 0.45 0.00 0.43 0.83 0.80 0.86
basal cell of prostate epithelium 325 0.74 0.02 0.86 0.41 0.00 0.24 0.89 0.82 0.87
pulmonary alveolar type 1 cell 296 0.97 0.96 0.96 0.81 0.00 0.86 0.97 0.96 0.98
pancreatic ductal cell 263 0.89 0.14 0.87 0.64 0.00 0.56 0.94 0.94 0.95
small intestine goblet cell 202 0.89 0.77 0.91 0.71 0.00 0.63 0.92 0.91 0.93
paneth cell of epithelium of small intestine 187 0.67 0.57 0.70 0.31 0.00 0.32 0.68 0.74 0.79
club cell 189 0.72 0.77 0.83 0.56 0.00 0.64 0.79 0.77 0.86
intestinal crypt stem cell of colon 170 0.78 0.53 0.69 0.46 0.00 0.10 0.78 0.80 0.85
progenitor cell of mammary luminal epithelium 182 0.99 0.00 0.98 0.98 0.00 0.96 0.96 0.95 0.99
epithelial cell 158 0.98 0.91 0.87 0.38 0.00 0.21 0.99 0.97 0.97
large intestine goblet cell 149 0.86 0.66 0.75 0.52 0.00 0.51 0.93 0.87 0.91
enterocyte of epithelium proper of small intestine 148 0.41 0.00 0.24 0.17 0.00 0.32 0.71 0.73 0.70
epithelial cell of uterus 141 0.96 0.47 0.79 0.37 0.00 0.25 0.99 0.95 0.98
acinar cell 140 0.86 0.56 0.52 0.68 0.00 0.68 0.93 0.92 0.94
lung ciliated cell 111 0.77 0.58 0.81 0.79 0.00 0.75 0.95 0.96 0.94
respiratory goblet cell 109 0.60 0.49 0.74 0.47 0.00 0.37 0.66 0.70 0.78
salivary gland cell 79 0.70 0.10 0.89 0.46 0.00 0.60 0.80 0.71 0.89
ciliated columnar cell of tracheobronchial tree 60 0.00 0.38 0.74 0.71 0.00 0.41 0.94 0.90 0.92
intrahepatic cholangiocyte 55 0.99 0.20 0.72 0.40 0.00 0.40 1.00 0.95 0.95
blood vessel smooth muscle cell 65 0.99 0.73 0.91 0.82 0.00 0.75 0.99 0.98 0.99
intestinal crypt stem cell of small intestine 43 0.00 0.21 0.33 0.16 0.00 0.18 0.35 0.36 0.40
myoepithelial cell 40 0.65 0.33 0.84 0.57 0.00 0.63 0.58 0.48 0.83
intestinal tuft cell 38 0.75 0.80 0.92 0.71 0.00 0.76 0.99 0.96 0.87
enterocyte of epithelium proper of jejunum 49 0.00 0.00 0.35 0.14 0.00 0.05 0.53 0.46 0.56
serous cell of epithelium of trachea 27 0.31 0.00 0.19 0.36 0.00 0.26 0.45 0.43 0.75
mesothelial cell 31 0.95 0.79 0.63 0.82 0.00 0.54 0.98 0.94 0.98
ciliated epithelial cell 27 0.91 0.00 0.46 0.72 0.00 0.57 1.00 0.93 0.93
mucus secreting cell 33 0.65 0.14 0.68 0.28 0.00 0.45 0.62 0.77 0.81
supporting cell of vestibular epithelium 27 0.90 0.00 0.71 0.78 0.00 0.54 1.00 0.96 0.98
enteroendocrine cell of small intestine 32 0.52 0.47 0.79 0.56 0.00 0.65 0.83 0.74 0.79
transit amplifying cell of colon 20 0.35 0.22 0.29 0.25 0.00 0.21 0.48 0.54 0.65
ovarian surface epithelial cell 14 0.90 0.12 0.67 0.56 0.00 0.31 1.00 0.86 0.96
tracheal goblet cell 24 0.00 0.19 0.65 0.59 0.00 0.41 0.81 0.63 0.79
paneth cell of colon 17 0.00 0.10 0.58 0.43 0.00 0.32 0.76 0.30 0.67
glandular epithelial cell 14 0.75 0.17 0.32 0.61 0.00 0.14 0.93 0.71 0.88
tuft cell of colon 19 0.00 0.09 0.76 0.27 0.00 0.61 0.88 0.92 0.67
ionocyte 10 0.80 0.53 0.67 0.67 0.00 0.50 0.83 0.71 0.86
neuro-medullary thymic epithelial cell 9 0.48 0.32 0.76 0.57 0.00 0.50 0.75 0.69 0.59
sebum secreting cell 0 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
type B pancreatic cell 15 0.00 0.00 0.90 0.82 0.00 0.54 0.97 0.88 0.97
myo-medullary thymic epithelial cell 4 0.00 0.00 0.00 1.00 0.00 0.05 1.00 1.00 1.00
interstitial cell of Cajal 7 0.00 0.00 0.92 0.62 0.00 0.31 0.93 0.92 1.00
retinal pigment epithelial cell 9 1.00 0.88 0.80 1.00 0.00 0.78 1.00 1.00 1.00
peritubular myoid cell 0 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
pancreatic A cell 10 0.00 0.00 0.90 0.43 0.00 0.80 1.00 0.89 1.00
vestibular dark cell 4 0.00 0.00 0.00 0.00 0.00 0.11 1.00 0.80 0.86
type L enteroendocrine cell 8 0.00 0.00 0.71 0.20 0.00 0.71 1.00 0.86 0.77
medullary thymic epithelial cell 4 0.00 0.00 0.00 0.00 0.00 0.05 0.89 0.75 0.67
transit amplifying cell of small intestine 4 0.00 0.00 0.40 0.00 0.00 0.08 0.00 0.00 0.00
taste receptor cell 4 0.00 0.00 0.00 0.00 0.00 0.10 0.00 0.22 0.40
enterochromaffin-like cell 5 0.00 0.00 0.57 0.20 0.00 0.60 0.75 0.80 0.57
pulmonary ionocyte 1 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
enteroendocrine cell 1 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
serous cell of epithelium of bronchus 1 0.00 0.00 0.00 0.00 0.00 0.06 1.00 1.00 1.00
pancreatic D cell 1 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
keratocyte 0 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
pancreatic PP cell 0 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
epithelial fate stem cell 1 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00

The train accuracies are computed on the training data.

Cell Type N cells celltypist knn bbknn knn harmony knn on scvi onclass scanvi svm xgboost Consensus Prediction
basal cell 30141 0.86 0.75 0.94 0.83 0.00 0.85 0.87 0.86 0.94
bladder urothelial cell 24511 0.99 0.88 0.94 0.79 0.00 0.97 1.00 0.99 1.00
luminal epithelial cell of mammary gland 13912 0.99 0.00 0.98 0.82 0.00 0.99 0.99 0.98 1.00
enterocyte of epithelium proper of duodenum 12117 0.92 0.08 0.88 0.70 0.00 0.94 0.92 0.92 0.97
stratified squamous epithelial cell 11133 0.83 0.78 0.93 0.86 0.00 0.82 0.85 0.84 0.90
acinar cell of salivary gland 11058 0.95 0.70 0.94 0.79 0.00 0.96 0.96 0.96 0.98
pulmonary alveolar type 2 cell 10411 0.96 0.96 0.98 0.83 0.00 0.97 0.98 0.97 0.98
conjunctival epithelial cell 9930 0.89 0.58 0.89 0.68 0.00 0.95 0.95 0.93 0.97
luminal cell of prostate epithelium 9564 0.92 0.39 0.96 0.81 0.00 0.96 0.96 0.94 0.97
kidney epithelial cell 8326 0.99 0.03 0.96 0.78 0.00 0.99 1.00 1.00 1.00
hepatocyte 6672 1.00 0.99 0.98 0.86 0.00 0.99 1.00 1.00 1.00
enterocyte of epithelium proper of ileum 5799 0.98 0.05 0.89 0.61 0.00 0.98 0.98 0.98 0.99
pancreatic acinar cell 4952 0.94 0.07 0.97 0.79 0.00 0.98 0.99 0.97 0.99
enterocyte of epithelium of large intestine 4092 0.78 0.27 0.79 0.62 0.00 0.87 0.84 0.88 0.86
corneal epithelial cell 3758 0.92 0.00 0.95 0.81 0.00 0.93 0.96 0.94 0.96
duct epithelial cell 3519 0.80 0.44 0.90 0.72 0.00 0.87 0.85 0.84 0.91
basal cell of prostate epithelium 3170 0.77 0.00 0.94 0.71 0.00 0.86 0.87 0.83 0.92
pulmonary alveolar type 1 cell 2820 0.97 0.95 0.98 0.88 0.00 0.94 0.98 0.96 0.98
pancreatic ductal cell 2456 0.90 0.03 0.94 0.80 0.00 0.96 0.97 0.95 0.97
small intestine goblet cell 1842 0.89 0.49 0.94 0.81 0.00 0.91 0.94 0.93 0.96
paneth cell of epithelium of small intestine 1666 0.65 0.36 0.80 0.56 0.00 0.68 0.76 0.77 0.85
club cell 1558 0.72 0.74 0.92 0.69 0.00 0.86 0.82 0.79 0.91
intestinal crypt stem cell of colon 1562 0.79 0.27 0.83 0.63 0.00 0.85 0.86 0.88 0.92
progenitor cell of mammary luminal epithelium 1512 0.98 0.00 0.99 0.98 0.00 0.95 0.95 0.94 0.99
epithelial cell 1476 0.95 0.41 0.82 0.61 0.00 0.96 0.98 0.97 0.98
large intestine goblet cell 1408 0.87 0.18 0.84 0.69 0.00 0.91 0.95 0.91 0.94
enterocyte of epithelium proper of small intestine 1267 0.42 0.00 0.74 0.43 0.00 0.85 0.74 0.78 0.87
epithelial cell of uterus 1274 0.98 0.12 0.90 0.55 0.00 0.94 0.99 0.96 0.99
acinar cell 1202 0.82 0.17 0.80 0.80 0.00 0.93 0.95 0.94 0.96
lung ciliated cell 1098 0.76 0.07 0.92 0.90 0.00 0.96 0.98 0.97 0.98
respiratory goblet cell 931 0.64 0.42 0.89 0.70 0.00 0.74 0.66 0.67 0.88
salivary gland cell 771 0.69 0.02 0.95 0.78 0.00 0.90 0.81 0.78 0.94
ciliated columnar cell of tracheobronchial tree 653 0.00 0.18 0.85 0.86 0.00 0.83 0.96 0.92 0.98
intrahepatic cholangiocyte 602 0.97 0.16 0.97 0.65 0.00 0.91 0.99 0.96 0.99
blood vessel smooth muscle cell 588 0.98 0.33 0.95 0.86 0.00 0.97 1.00 1.00 1.00
intestinal crypt stem cell of small intestine 544 0.01 0.13 0.48 0.30 0.00 0.39 0.60 0.62 0.71
myoepithelial cell 446 0.77 0.14 0.94 0.84 0.00 0.75 0.71 0.57 0.92
intestinal tuft cell 410 0.77 0.82 0.97 0.86 0.00 0.95 0.99 0.98 0.96
enterocyte of epithelium proper of jejunum 356 0.00 0.00 0.66 0.31 0.00 0.95 0.57 0.51 0.95
serous cell of epithelium of trachea 299 0.44 0.00 0.88 0.63 0.00 0.49 0.55 0.62 0.80
mesothelial cell 294 0.89 0.67 0.86 0.82 0.00 0.82 0.97 0.97 0.96
ciliated epithelial cell 293 0.95 0.00 0.79 0.78 0.00 0.87 0.99 0.98 0.99
mucus secreting cell 237 0.58 0.04 0.88 0.69 0.00 0.78 0.69 0.78 0.88
supporting cell of vestibular epithelium 240 0.87 0.00 0.69 0.82 0.00 0.95 1.00 0.97 1.00
enteroendocrine cell of small intestine 232 0.59 0.55 0.92 0.79 0.00 0.81 0.95 0.90 0.91
transit amplifying cell of colon 217 0.41 0.15 0.59 0.54 0.00 0.58 0.68 0.73 0.85
ovarian surface epithelial cell 216 0.90 0.06 0.85 0.73 0.00 0.90 1.00 0.97 0.99
tracheal goblet cell 203 0.00 0.17 0.90 0.71 0.00 0.73 0.86 0.71 0.92
paneth cell of colon 200 0.00 0.14 0.74 0.64 0.00 0.68 0.77 0.44 0.90
glandular epithelial cell 201 0.84 0.05 0.77 0.64 0.00 0.74 0.99 0.89 0.99
tuft cell of colon 159 0.00 0.03 0.94 0.64 0.00 0.96 0.98 0.93 0.97
ionocyte 150 0.71 0.34 0.83 0.73 0.00 0.72 0.87 0.83 0.88
neuro-medullary thymic epithelial cell 115 0.49 0.04 0.90 0.81 0.00 0.79 0.94 0.92 0.91
sebum secreting cell 106 0.76 0.00 0.91 0.34 0.00 0.84 0.97 0.83 0.97
type B pancreatic cell 87 0.00 0.00 0.93 0.91 0.00 0.80 1.00 1.00 0.99
myo-medullary thymic epithelial cell 73 0.00 0.00 0.95 0.87 0.00 0.67 1.00 0.99 0.99
interstitial cell of Cajal 63 0.00 0.00 1.00 0.88 0.00 0.71 0.98 0.99 1.00
retinal pigment epithelial cell 58 0.98 0.03 1.00 0.94 0.00 0.95 0.99 0.99 1.00
peritubular myoid cell 61 0.00 0.00 0.74 0.60 0.00 0.98 0.99 0.91 0.97
pancreatic A cell 38 0.00 0.00 0.96 0.88 0.00 0.88 1.00 1.00 1.00
vestibular dark cell 41 0.00 0.00 0.70 0.27 0.00 0.20 1.00 0.88 0.97
type L enteroendocrine cell 36 0.00 0.00 0.83 0.44 0.00 0.83 0.99 0.96 0.94
medullary thymic epithelial cell 31 0.00 0.00 0.85 0.40 0.00 0.43 0.91 0.87 0.98
transit amplifying cell of small intestine 30 0.00 0.00 0.45 0.06 0.00 0.28 0.75 0.83 0.91
taste receptor cell 28 0.00 0.00 0.53 0.63 0.00 0.36 0.81 0.47 0.89
enterochromaffin-like cell 22 0.00 0.00 0.84 0.67 0.00 0.86 0.98 1.00 0.98
pulmonary ionocyte 24 0.00 0.00 0.89 0.59 0.00 0.51 1.00 0.81 0.98
enteroendocrine cell 24 0.00 0.00 0.88 0.73 0.00 0.62 0.92 0.94 0.92
serous cell of epithelium of bronchus 14 0.00 0.00 0.73 0.53 0.00 0.42 0.97 1.00 0.92
pancreatic D cell 8 0.00 0.00 0.57 0.86 0.00 0.67 1.00 1.00 1.00
keratocyte 7 0.00 0.00 0.25 0.00 0.00 0.54 0.82 0.33 0.44
pancreatic PP cell 2 0.00 0.00 0.00 0.00 0.00 0.80 1.00 1.00 0.67
epithelial fate stem cell 1 0.00 0.00 0.00 0.00 0.00 0.14 0.50 1.00 0.00

References

Tabula Sapiens reveals transcription factor expression, senescence effects, and sex-specific features in cell types from 28 human organs and tissues, The Tabula Sapiens Consortium; bioRxiv, doi: https://doi.org/10.1101/2024.12.03.626516

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