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} | ['gnd:4057696-6', 'gnd:4432839-4'] | ['Stoffübertragung', 'Bodenphysik'] | ['hor'] | ['Technische Informationsbibliothek (TIB)'] | [['(ppn)725367784', '(firstid)GBV:725367784', '(doi)10.25673/754']] | ['Universitäts- und Landesbibliothek Sachsen-Anhalt'] | [['(classificationName=ddc-dbn)510', '(classificationName=bk, id=106409069)48.32 - Bodenkunde, Bodenbewertung', '(classificationName=linseach:mapping)hor']] | ['Quantification of soil structural heterogeneity and its impact on flow and transport'] | [['Flow and transport in the subsurface are determined by the structural heterogeneity of natural porous media. Connectivity is known to be an important facet of structural heterogeneity that has to be captured adequately in order to facilitate or improve model predictions on diverse physical processes in porous formations. This thesis presents some novel insights into the approaches to quantify structural connectivity and the complex relationship between structural and functional connectivity in the realm of vadose zone hydrology. Functional connectivity is expressed as effective hydraulic behavior with respect to stationary flow, solute transport and hydraulic non-equilibrium during infiltration. Numerical simulations demonstrate that a good reproduction of structural connectivity also entails a good reproduction of functional connectivity metrics. However, structural connectivity affect upscaled flow, transport and hydraulic non-equilibrium behavior differently. Thus, functional connectivity has to be considered as a process and state-dependent concept.', 'connectivity metrics; stochastic reconstruction; stationary flow; solute transport; hydraulic nonequilibrium; upscaling', 'Fluss und Transport in natürlichen porösen Medien werden durch die Strukturheterogenität des Untergrunds bestimmt. Konnektivität ist ein wichtiger Aspekt jener Strukturheterogenität, der richtig erfasst werden muss, um Modelvorhersagen zu verschiedenen physikalischen Prozessen zu ermöglichen oder zu verbessern. Die vorliegende Arbeit präsentiert neue Erkenntnisse über die quantitative Beschreibbarkeit von Strukturkonnektivität und die komplexe Beziehung zwischen struktureller und funktionaler Konnektivität in Bezug auf die Hydrologie der ungesättigten Zone. Funktionale Konnektivität beschreibt dabei das effektive hydraulische Verhalten in Bezug auf stationären Fluss, Stofftransport und hydraulisches Ungleichgewicht. Numerische Simulationen zeigen, dass eine gute Reproduktion von Strukturkonnektivität auch zu einer guten Reproduktionen funktionaler Konnektivität führt. Strukturkonnektivität beeinflusst jedoch effektiven Fluss, Transport und hydraulisches Ungleichgewicht auf unterschiedliche Weise. Funktionale Konnektivität muss daher als ein prozess- und zustandsabhängiges Konzept aufgefasst werden.', 'Konnektivitätsmaße; stochastische Rekonstruktion; stationärer Fluss; Stofftransport; hydraulisches Ungleichgewicht; Upscaling']] | ['2012'] | ['http://id.loc.gov/vocabulary/iso639-1/de'] | ['commercial licence'] | [[{'@id': 'gnd:4057696-6'}, {'@id': 'gnd:4432839-4'}]] | ['gnd:1205349006', 'gnd:4057696-6', 'gnd:4432839-4', 'https://www.tib.eu/de/suchen/id/TIBKAT%3A725367784'] | ['Schlüter, Steffen', 'Stoffübertragung', 'Bodenphysik'] | 10 |
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The work aims, on both theoretical-analytical and methodical-empirical level, to create a first approach to the scientific development of the hitherto little-explored area of the game picture book game. This includes a definitional clarification as well as a detailed investigation of the various manifestations of this genre. The work focuses on those picture books that prompt to motor activities such as drawing, turning, flipping, or playing, and offer exercise- and gaming effects and thereby support, accompany and expand the narrative story or the theme of the picture book. For the selected and in the work described and analyzed game picture books differentiated criteria were developed. The analysis of the different manifestations of the game picture books works out the characteristic features, different mechanisms and peculiarities of this sort of book, and relates them to their aesthetic learning potential for children. 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Die Arbeit verfolgt das Ziel, sowohl auf theoretisch-analytischer als auch auf methodisch-empirischer Ebene einen ersten Ansatz zur wissenschaftlichen Erschließung des bislang wenig erforschten Bereichs des Spielbilderbuchs zu erstellen. Das schließt eine definitorische Klärung ebenso ein wie eine differenzierte Untersuchung der unterschiedlichen Erscheinungsformen dieser Gattung. Die Arbeit konzentriert sich auf solche Bilderbücher, die zu motorischen Tätigkeiten wie Ziehen, Drehen, Umklappen oder Spielen auffordern und Bewegungs- und Spieleffekte anbieten und dabei die narrative Geschichte oder das Thema des Bilderbuches unterstützen, begleiten und erweitern. Für die ausgewählten und in der Arbeit beschriebenen und analysierten Spielbilderbücher wurden differenzierte Kriterien entwickelt. Die Analyse der unterschiedlichen Erscheinungsformen der Spielbilderbücher arbeitet die charakteristischen Merkmale, unterschiedlichen Mechaniken und Besonderheiten des Spielbilderbuches heraus und bezieht sie auf ihr ästhetisches Lernpotenzial für Kinder. Die gewonnenen Erkenntnisse der Arbeit zeigen, dass ein Spielbilderbuch nicht lediglich eine Zwischenform zwischen Bilderbuch und Spielzeug ist, sondern einen komplexen Literaturgegenstand darstellt, der sich zwischen Kunst, Buch und Spielzeug, zwischen Erzählungen, Sachthemen, didaktischen und pädagogischen Ansprüchen bewegt und entfaltet. Hinter den unterschiedlichen Erscheinungsformen des Spielbilderbuchs lässt sich ein beachtliches Potenzial an bildnerischen, plastischen und kinetischen Gestaltungsformen erkennen, das vielfältige ästhetische Erfahrungs- und Lernmöglichkeiten bietet. <dt.>']] | ['2012'] | ['http://id.loc.gov/vocabulary/iso639-1/de'] | ['commercial licence'] | [[{'@id': 'gnd:4006604-6'}, {'@id': 'gnd:4031081-4'}, {'@id': 'gnd:4395663-4'}, {'@id': 'gnd:4056458-7'}, {'@id': 'gnd:4345598-0'}]] | ['gnd:1025065751', 'gnd:120856808', 'gnd:4006604-6', 'gnd:4031081-4', 'gnd:4056458-7', 'gnd:4345598-0', 'gnd:4395663-4', 'https://www.tib.eu/de/suchen/id/TIBKAT%3A725644133'] | ['Al Chammas, Tamara', 'Thiele, Jens', 'Bilderbuch', 'Kleinkind', 'Spracherwerb', 'Spielbilderbuch', 'Pop-up-Technik'] | 13 |
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The work aims, on both theoretical-analytical and methodical-empirical level, to create a first approach to the scientific development of the hitherto little-explored area of the game picture book game. This includes a definitional clarification as well as a detailed investigation of the various manifestations of this genre. The work focuses on those picture books that prompt to motor activities such as drawing, turning, flipping, or playing, and offer exercise- and gaming effects and thereby support, accompany and expand the narrative story or the theme of the picture book. For the selected and in the work described and analyzed game picture books differentiated criteria were developed. The analysis of the different manifestations of the game picture books works out the characteristic features, different mechanisms and peculiarities of this sort of book, and relates them to their aesthetic learning potential for children. 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"In plant seeds, the micronutrients Fe and Zn are required to sustain the early development of seedlings. A large part of the Fe and Zn in the seeds derives from retranslocation from senescing leaves or stems. Nitrogen nutrition has been reported to play an important role in micronutrient retranslocation to grains by stimulating the synthesis of Fe chelators like nicotianamine and phytosiderophores. The present study assessed the effect of different N fertilizer forms on leaf senescence and on micronutrient accumulation in wheat grains (Triticum aestivum cv. Akteur) under field conditions in two consecutive years. Flag leaf senescence was delayed by N fertilization, and in both field experiments the supply of ammonium after anthesis resulted in higher Fe and Zn contents in the grains. Nicotianamine levels increased in leaves and leaf exudates, whereas phytosiderophore concentrations were less responsive to N treatments. To enhance Fe and Zn mobilization, citric acid was sprayed onto senescing leaves of wheat. In N-fertilized plants citric acid application did not increase Fe and Zn accumulation in grains. However, in non-fertilized plants citric acid application increased Fe and Zn remobilization and led to significantly elevated Fe contents in the grains. The results of the present study indicate that N fertilization can improve the fortification of wheat grains with Fe and Zn, in particular when the different effects of fertilizer N forms are respected, while citric acid application could not be proven efficient to increase Fe and Zn accumulation in grains.",
"Triticum aestivum; nicotianamine; phytosiderophore; senescence; chelator; citric acid; micronutrients; nitrate; ammonium; urea",
"Triticum aestivum; Ncotianamin; Phytosiderophore; Seneszenz; Chelatoren; Zitronens\u00e4ure; Mikron\u00e4hrstoffe; Nitrat; Ammonium; Harnstoff",
"In Getreidesamen werden die Mikron\u00e4hrstoffe Fe und Zn f\u00fcr die fr\u00fche Entwicklung des Keimlings ben\u00f6tigt. Ein gro\u00dfer Teil dieser Mikron\u00e4hrstoffe stammt aus der Retranslokation von Fe und Zn aus seneszierenden Bl\u00e4ttern. F\u00fcr die Retranslokation von Fe und Zn spielt die Stickstoffern\u00e4hrung eine wichtige Rolle, da Stickstoff (N) die Synthese von N-haltigen Schwermetall-Chelatoren wie Nicotianamin und Phytosiderophoren stimuliert. Die vorliegende Arbeit hatte zum Ziel, den Einfluss unterschiedlicher N-D\u00fcngemittelformen auf die Blattseneszenz und Mikron\u00e4hrstoff-anreicherung in Weizenk\u00f6rnern (Triticum aestivum cv. Akteur) unter Feldbedingungen zu untersuchen. Es zeigte sich, dass die Seneszenz der Fahnenbl\u00e4tter durch N-D\u00fcngung verz\u00f6gert wurde und dass v.a. eine D\u00fcngung mit Ammonium nach der Bl\u00fcte zu erh\u00f6hten Fe- und Zn-Gehalten in den K\u00f6rnern f\u00fchrte. Stickstoffapplikation f\u00fchrte auch zu erh\u00f6hten Nicotianamingehalten in Bl\u00e4ttern und Blattexudaten, wogegen die Phytosiderophorkonzentrationen weit weniger beeinflusst wurden. Um die Fe- und Zn-Retranslokation zu erh\u00f6hen, wurde in einem weiteren Versuch Zitronens\u00e4ure auf seneszierende Bl\u00e4tter von Freilandweizen gespr\u00fcht. In N-ged\u00fcngten Pflanzen ver\u00e4nderte die Blattapplikation von Zitronens\u00e4ure allerdings nicht die Fe- und Zn-Anreicherung in den Samen, wogegen in unged\u00fcngten Pflanzen die Fe- und Zn-Gehalte in den Samen signifikant anstiegen. Die Ergebnisse dieser Arbeit zeigen, dass N-D\u00fcngung die Fe- und Zn-Anreicherung in Weizensamen verbessern kann und zwar besonders dann, wenn unterschiedliche Effekte der N-D\u00fcngemittelform ber\u00fccksichtigt werden, w\u00e4hrend f\u00fcr eine Blattapplikation von Zitronens\u00e4ure keine positive Wirkung auf die Retranslokation von Fe oder Zn nachgewiesen werden konnte."
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} | [] | [] | ['inf'] | ['gnd:110026063'] | [['(firstid)HBZ:CT004000968', '(ppn)780183738']] | [''] | [['(classificationName=ddc)005', '(classificationName=ddc-dbn)004', '(classificationName=linseach:mapping)inf']] | ['Multi-Algorithmen-Rendering : Darstellung heterogener 3-D-Szenen in Echtzeit : Real-time 3D rendering of heterogeneous scenes'] | [['Many large virtual 3D scenes are not structured evenly, for example because they exhibit a highly varying density distribution of their polygons. For such heterogeneous data, there is no single algorithm that constantly performs well with any type of scene and that is able to render the scene at each position fast with the same high image quality. For a small set of scenes, this situation can be improved, if an experienced user is able to manually assign different rendering algorithms to particular parts of the scene. We introduce the Multi- Algorithm-Rendering method which automatically deploys different rendering algorithms simultaneously for a broad range of scene types. The method divides the scene into suitable subregions and measures the behavior of the different algorithms for each region in a preprocessing step. During runtime, this data is utilized to compute an estimate for the quality and running time of the available rendering algorithms from the observers point of view. By solving an optimizing problem embedded in a control cycle, the image quality can be optimized by the appropriate assignment of algorithms to regions, while keeping the achieved frame rate almost constant. In an experimental evaluation, we compare our methods running time and image quality with that of standard rendering algorithms.', 'Viele große virtuelle 3-D-Szenen sind nicht gleichmäßig strukturiert, z. B. weil sie eine stark schwankende Dichteverteilung der Geometrie aufweisen. Für solche ungleichmäßig strukturierten Szenen gibt es keinen Algorithmus, der an allen Betrachterpositionen gut funktioniert, also für beliebige Positionen ein Bild mit guter Qualität in annehmbarer Zeit darstellt. Für eine kleine Teilmenge dieser Szenen kann die Situation verbessert werden, indem ein erfahrener Benutzer per Hand für einzelne Bereiche einer Szene entscheidet, mit welchem Algorithmus diese dargestellt werden. In dieser Arbeit wird ein Verfahren vorgestellt, welches automatisch unterschiedliche Algorithmen gleichmäßig strukturierten Bereichen einer Szene zuordnet. Die Methode teilt dafür die Szene in brauchbare Bereiche auf und misst das Verhalten unterschiedlicher Algorithmen auf diesen Bereichen in einem Vorberechnungsschritt. Zur Laufzeit werden diese Daten dann genutzt, um eine Vorhersage für die Laufzeit und den entstehenden Bildfehler der einzelnen Algorithmen auf den Bereichen der Szene abhängig von der Position des Betrachters zu erhalten. Mittels Lösens eines in einen Regelkreis eingebetteten Optimierungsproblems kann dann die Bildqualität durch eine geschickte Zuordnung von Algorithmen zu Bereichen bei nahezu konstanter Bildrate optimiert werden. In einer experimentellen Evaluierung werden sowohl Laufzeit als auch Bildqualität unserer Methode mit denen von Standardverfahren verglichen.']] | ['2014'] | ['http://id.loc.gov/vocabulary/iso639-1/de'] | ['commercial licence'] | [''] | ['gnd:110026063', 'gnd:4219666-8', 'https://www.tib.eu/de/suchen/id/TIBKAT%3A780183738'] | ['Meyer auf der Heide, Friedhelm', 'Rendering'] | 11 |
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"Due to growing individual transport in industrial countries and associated increase in pollutant emissions, legislature toughens emission standards regularly. Automakers are therefor challenged to develop ever more efficient low-emission vehicles to take account of the public climate debate and effects of pollutants on humans and nature. Conventional exhaust gas measurement systems are limited when it comes to measuring gases other than O2, CO2, CO, NOX and total-HC or when high measurement frequencies are of the essence to resolve transient processes. Additional specialized single component measurement systems increase the already high complexity of conventional exhaust emission measurement devices. Topic of this work was the study of all aspects of a mobile, versatile and dynamic exhaust gas measurement system, which integrates the multi component measurement systems mass spectrometer and Fourier-Transform infrared spectrometer. Analyzers were specifically modified and optimized for this application, a common sampling system was designed, and a corresponding software structure was realized. Methods were researched for the optimal adjustment of the system to different applications and for increasing system stability as well as minimizing calibration effort by measuring combustion products like CO2 and H2O and other components with both analyzers in parallel. Additionally Argon is used as some kind of internal standard to adjust and stabilize the mass spectrometer. Measurements in the laboratory and on gasoline and diesel engines show, that limited and non-limited as well as other exhaust gas components can be detected in concentrations down to 1 ppm and with up to 20 Hz. As exemplary applications the measurement of all main exhaust gas components, the differentiation of hydro carbons, the behavior of nitrogen oxides and ammonia on a SCR catalytic converter, the detection of hydrogen in gasoline engine exhaust and the determination of exhaust mass flow rate using Helium as tracer gas are demonstrated. This work shows the advantages of the hybrid measurement system over conventional exhaust emission measurement devices. Higher measurement frequency and much broader spectrum of detectable gas components open a very wide field of application. Lower calibration effort and compact design allow an efficient operation on a regular basis. It can be advantageous deployed to solve current and future problems in exhaust gas measurement as well as outside this field of application.",
"Um der Zunahme von Schadstoffemissionen durch den stetig wachsenden motorisierten Individualverkehr in Industrienationen entgegenzuwirken, werden seitens der Gesetzgeber Emissionsgrenzwerte regelm\u00e4\u00dfig versch\u00e4rft. Automobilhersteller sind daher gefordert, immer effizientere und schadstoff\u00e4rmere Fahrzeuge zu entwickeln. Konventionelle Abgasmesstechniken sto\u00dfen bei aktuellen Fragestellungen an ihre Grenze, wenn neben O2, CO2, CO, NOX und Gesamt-HC weitere Stoffe gemessen, oder mit einer hohen Messfrequenz transiente Vorg\u00e4nge abgebildet werden m\u00fcssen. Durch den Einsatz zus\u00e4tzlicher, spezieller Einkomponenten-Messsysteme wird die bereits hohe Komplexit\u00e4t der konventionellen Abgasmessanlagen weiter gesteigert. Gegenstand dieser Arbeit war die Untersuchung ger\u00e4tetechnischer und methodischer Aspekte eines mobilen, universellen und dynamischen Abgasmesssystems, das die bei- den an Pr\u00fcfst\u00e4nden etablierten Mehrkomponenten-Messsysteme Massenspektrometer und Fourier-Transform-Infrarot-Spektrometer integriert. Dazu wurden Analysatoren speziell f\u00fcr diese Anwendung modifiziert und optimiert, eine gemeinsame Probenahme ausgelegt und eine entsprechende Softwarestruktur realisiert. Es sind Methoden untersucht worden, mit denen das hybride Messsystem optimal f\u00fcr unterschiedliche Anwendungen einzustellen ist, durch parallele Messungen der Verbrennungsprodukte CO2 und H2O sowie weiterer Stoffe die Systemstabilit\u00e4t gesteigert und der Kalibrieraufwand minimiert wird. Argon kann dabei als eine Art interner Standard zur Einstellung und Stabilisierung des Massenspektrometers genutzt werden. Messungen im Labor sowie an Otto- und Dieselmotoren zeigen, dass limitierte und nicht- limitierte Schadstoffe sowie weitere Abgaskomponenten ab Konzentrationen von ca. 1 ppm mit bis zu 20 Hz gemessen werden k\u00f6nnen. Als beispielhafte Anwendungen werden die Messung der Abgas-Hauptkomponenten, die Differenzierung von Kohlenwasserstoffen, das Verhalten von Stickoxiden und Ammoniak am SCR-Katalysator, der Nachweis von Wasserstoff im Ottoabgas sowie die Bestimmung des Abgasmassenstroms mittels Helium als Tracergas aufgezeigt. Mit dieser Arbeit wird gezeigt, dass das hybride Abgasmesssystem viele Vorz\u00fcge gegen\u00fcber der konventionellen Abgasmesstechnik hat. Die h\u00f6here Messfrequenz sowie das viel breitere Spektrum messbarer Stoffe er\u00f6ffnen einen sehr gro\u00dfen Einsatzbereich, und der geringere Kalibrieraufwand sowie die kompakte Bauweise erlauben einen effizienten Routinebetrieb. Es kann f\u00fcr aktuelle und zuk\u00fcnftige Fragestellungen der Abgasmesstechnik, aber auch au\u00dferhalb dieses Anwendungsgebiets gewinnbringend eingesetzt werden."
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Automakers are therefor challenged to develop ever more efficient low-emission vehicles to take account of the public climate debate and effects of pollutants on humans and nature. Conventional exhaust gas measurement systems are limited when it comes to measuring gases other than O2, CO2, CO, NOX and total-HC or when high measurement frequencies are of the essence to resolve transient processes. Additional specialized single component measurement systems increase the already high complexity of conventional exhaust emission measurement devices. Topic of this work was the study of all aspects of a mobile, versatile and dynamic exhaust gas measurement system, which integrates the multi component measurement systems mass spectrometer and Fourier-Transform infrared spectrometer. Analyzers were specifically modified and optimized for this application, a common sampling system was designed, and a corresponding software structure was realized. Methods were researched for the optimal adjustment of the system to different applications and for increasing system stability as well as minimizing calibration effort by measuring combustion products like CO2 and H2O and other components with both analyzers in parallel. Additionally Argon is used as some kind of internal standard to adjust and stabilize the mass spectrometer. Measurements in the laboratory and on gasoline and diesel engines show, that limited and non-limited as well as other exhaust gas components can be detected in concentrations down to 1 ppm and with up to 20 Hz. As exemplary applications the measurement of all main exhaust gas components, the differentiation of hydro carbons, the behavior of nitrogen oxides and ammonia on a SCR catalytic converter, the detection of hydrogen in gasoline engine exhaust and the determination of exhaust mass flow rate using Helium as tracer gas are demonstrated. This work shows the advantages of the hybrid measurement system over conventional exhaust emission measurement devices. Higher measurement frequency and much broader spectrum of detectable gas components open a very wide field of application. Lower calibration effort and compact design allow an efficient operation on a regular basis. It can be advantageous deployed to solve current and future problems in exhaust gas measurement as well as outside this field of application.', 'Um der Zunahme von Schadstoffemissionen durch den stetig wachsenden motorisierten Individualverkehr in Industrienationen entgegenzuwirken, werden seitens der Gesetzgeber Emissionsgrenzwerte regelmäßig verschärft. Automobilhersteller sind daher gefordert, immer effizientere und schadstoffärmere Fahrzeuge zu entwickeln. Konventionelle Abgasmesstechniken stoßen bei aktuellen Fragestellungen an ihre Grenze, wenn neben O2, CO2, CO, NOX und Gesamt-HC weitere Stoffe gemessen, oder mit einer hohen Messfrequenz transiente Vorgänge abgebildet werden müssen. Durch den Einsatz zusätzlicher, spezieller Einkomponenten-Messsysteme wird die bereits hohe Komplexität der konventionellen Abgasmessanlagen weiter gesteigert. Gegenstand dieser Arbeit war die Untersuchung gerätetechnischer und methodischer Aspekte eines mobilen, universellen und dynamischen Abgasmesssystems, das die bei- den an Prüfständen etablierten Mehrkomponenten-Messsysteme Massenspektrometer und Fourier-Transform-Infrarot-Spektrometer integriert. Dazu wurden Analysatoren speziell für diese Anwendung modifiziert und optimiert, eine gemeinsame Probenahme ausgelegt und eine entsprechende Softwarestruktur realisiert. Es sind Methoden untersucht worden, mit denen das hybride Messsystem optimal für unterschiedliche Anwendungen einzustellen ist, durch parallele Messungen der Verbrennungsprodukte CO2 und H2O sowie weiterer Stoffe die Systemstabilität gesteigert und der Kalibrieraufwand minimiert wird. Argon kann dabei als eine Art interner Standard zur Einstellung und Stabilisierung des Massenspektrometers genutzt werden. Messungen im Labor sowie an Otto- und Dieselmotoren zeigen, dass limitierte und nicht- limitierte Schadstoffe sowie weitere Abgaskomponenten ab Konzentrationen von ca. 1 ppm mit bis zu 20 Hz gemessen werden können. Als beispielhafte Anwendungen werden die Messung der Abgas-Hauptkomponenten, die Differenzierung von Kohlenwasserstoffen, das Verhalten von Stickoxiden und Ammoniak am SCR-Katalysator, der Nachweis von Wasserstoff im Ottoabgas sowie die Bestimmung des Abgasmassenstroms mittels Helium als Tracergas aufgezeigt. Mit dieser Arbeit wird gezeigt, dass das hybride Abgasmesssystem viele Vorzüge gegenüber der konventionellen Abgasmesstechnik hat. Die höhere Messfrequenz sowie das viel breitere Spektrum messbarer Stoffe eröffnen einen sehr großen Einsatzbereich, und der geringere Kalibrieraufwand sowie die kompakte Bauweise erlauben einen effizienten Routinebetrieb. Es kann für aktuelle und zukünftige Fragestellungen der Abgasmesstechnik, aber auch außerhalb dieses Anwendungsgebiets gewinnbringend eingesetzt werden.']] | ['2013'] | ['http://id.loc.gov/vocabulary/iso639-1/de'] | ['open access'] | [[{'@id': 'gnd:4511937-5'}, {'@id': 'gnd:4169043-6'}, {'@id': 'gnd:4161702-2'}, {'@id': 'gnd:4000123-4'}]] | ['gnd:10195311-2', 'gnd:1161951148', 'gnd:137795831', 'gnd:4000123-4', 'gnd:4161702-2', 'gnd:4169043-6', 'gnd:4511937-5', 'https://www.tib.eu/de/suchen/id/TIBKAT%3A781214912'] | ['Technische Universität Hamburg-Harburg, Institut für Messtechnik', 'Matz, Gerhard', 'Thiemann, Wolfgang', 'Abgas', 'Infrarotspektrometer', 'Massenspektrometer', 'Online-Ressource'] | 15 |
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"title": "Impact of gallium on the defect landscape of sulfur-based chalcopyrite solar cells",
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"Die Leerlaufspannung von Solarzellen basierend auf Cu(In,Ga)S2 wird durch Rekombinationsverluste \u00fcber Grenzfl\u00e4chendefekte limitiert. Erh\u00f6hte Werte f\u00fcr die Leerlaufspannung lassen sich durch erh\u00f6hte Ga-Konzentrationen erzielen. Dabei ist \u00fcber den Einfluss von Ga auf die Energieb\u00e4nder in Schwefel-basierten Chalcopyriten bisher wenig bekannt. In dieser Arbeit werden Cu(In,Ga)S2 Solarzellen mit unterschiedlichen Ga-Tiefenprofilen in der Absorberschicht mit Hinblick auf den Einfluss von Ga auf Solarzellenparameter und Defekteigenschaften mit Hilfe elektro-optischer Methoden untersucht. Die ermittelten Zusammenh\u00e4nge geben Aufschluss auf den Einfluss von Ga auf die Energieb\u00e4nder in Cu(In,Ga)S2 Solarzellen. Dar\u00fcber hinaus werden die Eigenschaften der mittels Admittanzspektroskopie nachgewiesenen Signaturen mit den Charakteristika der in Cu(In,Ga)Se2 Solarzellen auftretenden Signaturen N1 und N2 verglichen. Auf diese Weise k\u00f6nnen die beobachteten Signaturen weiter klassifiziert werden. <dt.>"
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} | ['gnd:4138579-2', 'gnd:4006896-1', 'gnd:4416206-6', 'gnd:7651795-0', 'gnd:4626532-6', 'gnd:4027110-9', 'gnd:4159584-1'] | ['Biorhythmus', 'Insekten', 'Metabolit', 'Herbivoren', 'Abwehrreaktion', 'Nicotiana attenuata', 'Mathematische Modellierung'] | ['bio'] | ['Technische Informationsbibliothek (TIB)'] | [['(firstid)GBV:782418570', '(ppn)782418570']] | [''] | [['(classificationName=bk, id=10640976X)42.41 - Pflanzenphysiologie', '(classificationName=ddc-dbn)590', '(classificationName=linseach:mapping)bio', '(classificationName=ddc-dbn)570', '(classificationName=ddc)571.9637423952']] | ['Integrative statistical methods for decoding molecular responses to insect herbivory in Nicotiana attenuata'] | ['This work focuses on the development of statistical methods to select features (genes and metabolites) exhibiting induced local and systemic defense responses to insect attack in Nicotiana attenuata along with the extraction of additional information regarding their timing of action. To characterize the dynamics of activation in time and space of herbivory-induced responses, I designed a framework by combining methods previously developed for feature selection and extraction to identify activated network motifs. These motifs are the set of features that are differentially perturbed in local and systemic tissues in response to herbivory. ...'] | ['2013'] | ['http://id.loc.gov/vocabulary/iso639-1/en'] | ['open access'] | [[{'@id': 'gnd:4138579-2'}, {'@id': 'gnd:4006896-1'}, {'@id': 'gnd:4416206-6'}, {'@id': 'gnd:7651795-0'}, {'@id': 'gnd:4626532-6'}, {'@id': 'gnd:4027110-9'}, {'@id': 'gnd:4159584-1'}]] | ['gnd:1047266288', 'gnd:36164-1', 'gnd:4006896-1', 'gnd:4027110-9', 'gnd:4138579-2', 'gnd:4159584-1', 'gnd:4416206-6', 'gnd:4626532-6', 'gnd:7651795-0', 'https://www.tib.eu/de/suchen/id/TIBKAT%3A782418570'] | ['Gulati, Jyotasana', 'Friedrich-Schiller-Universität Jena', 'Biorhythmus', 'Insekten', 'Metabolit', 'Herbivoren', 'Abwehrreaktion', 'Nicotiana attenuata', 'Mathematische Modellierung'] | 3 |
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In particular for systems biology studies, e.g. to describe the interplay of metabolic reactions and their dynamics, a reliable metabolomic analysis under physiological or in vivo conditions is required. While genomic, proteomic, and transcriptomic data can be obtained with high accuracy nowadays, the determination of metabolic profiles and fluxes still proves to be complicated. On the one hand, this is due to the fast turnover rates, the interaction between various metabolic pathways, and the chemical diversity of metabolites. On the other hand, the compartmentalization of metabolism in mammalian cells poses a huge challenge because of the dynamic range of metabolite concentration within the cytosol and the mitochondria. To overcome these limitations of metabolomic analysis, attempts have been made in this work to achieve a fast and efficient separation of the cytosolic and mitochondrial compartments by using new technological approaches in both macro and microscales. Due to its significant value, the well-known industrial cell line CHO-K1 was used as a model organism within this research project. To investigate the isolation of mitochondria in macroscale, different cell disruption methods were modified and tested. The efficiency and sensitivity of each were determined by the measure of mitochondrial yield and the integrity of isolated mitochondria. Here, the highest yields of intact mitochondria were obtained using ultrasound for cell disruption and subsequent differential centrifugation. In addition to the large-scale procedures, a microfluidic device for metabolic analysis, providing a complete sample preparation (including cell disruption) within seconds, was developed in project cooperation. In this work, a prototype of the Lab-on-a-Chip (LoaC) was successfully integrated into a bioreactor and operated for a period of 18 h. Continuous sampling was carried out by applying overpressure onto the bioreactor. Besides dynamic pulse experiments, this microchip-bioreactor system implemented an efficient separation of cells and extracellular media. Moreover, a selective cell lysis using digitonin and a subsequent separation of released cytosol from the remaining cellular and mitochondrial compartments was realised on-chip. Thus, this integrated microfluidic system provides high potential to overcome the limitations of analyses concerning compartmentalized metabolism. In the last part of this work, CHO-K1 cells were cultivated in a controlled small-scale bioreactor in order to perform systems biology studies. Therefore, detailed analyses of extracellular metabolites using LC-MS were performed under batch and continuous cultivation conditions. In addition, the mitochondrial structure and distribution of mitochondrial proteins were studied using confocal and high resolution STED microscopy. Furthermore, the relative level of enzymes involved in metabolic reactions was measured intracellularly by using the in-cell ELISA technology. To investigate the dependence of metabolic reactions on different cell cycle phases, the cultivation of synchronized cells was performed. For this purpose, cells from heterogeneous populations were enriched in the G1- and S-phase, respectively, using the centrifugal elutriation method. The synchrony during batch cultivation was determined flow cytometrically by analysing the DNA content and the cell size distribution. In addition, the cell count was observed continuously due to the effective integration of an at-line microscope. Metabolomic and microscopic studies of synchronized cells were performed during cultivation and the resultant data were compared to that obtained during cultivation of heterogeneous cell populations. The cell cycle dependency of metabolism was shown by the increased consumption rates of glucose and glutamine within the S-phase and the shifted increased lactate production during G1-phase, amongst others.', 'Metabolomische Analysen bilden einen entscheidenden Schritt zum Verständnis zellulärer Funktionen auf genomischer Ebene. Verlässliche metabolomische Analysen unter physiologischen oder in vivo Bedingungen werden insbesondere für systembiologische Untersuchungen zur Beschreibung der Interaktionen von Stoffwechselreaktionen und ihrer Dynamik benötigt. Während genomische, proteomische und transkriptomische Daten heutzutage mit hoher Präzision generiert werden können, gestaltet sich die Bestimmung von Metabolitenprofilen nach wie vor schwierig. Dies liegt einerseits an den schnellen Umsatzraten, den Wechselwirkungen zwischen verschiedenen Stoffwechselwegen und der chemischen Diversität von Metaboliten. Auf der anderen Seite bildet die Kompartimentierung des Stoffwechsels tierischer Zellen eine große Herausforderung, da hierdurch eine dynamische Spannbreite von Metabolitkonzentrationen innerhalb des Zytosols und der Mitochondrien vorliegt. Zur Bewältigung der Schwierigkeiten der Zellkompartimentierung wurde in der vorliegenden Arbeit der Versuch unternommen, die schnelle und effiziente Separation zytosolischer und mitochondrialer Kompartimente von Säugetierzellen durch neue technologische Ansätze sowohl im Makromaßstab als auch im Mikromaßstab, auf einem Lab-on-a-Chip (LoaC), zu erzielen. Wegen ihrer beachtlichen industriellen Bedeutung wurde innerhalb dieser Forschungsarbeit die Suspensionszelllinie CHO-K1 als Modell-organismus verwendet. Für die Mitochondrienisolation im Makromaßstab wurden verschiedene Zellaufschlussverfahren modifiziert und getestet. Die Effizienz und Sensitivität einer jeden Methode wurden durch die Messung der Mitochondrienausbeute und der Integrität isolierter Mitochondrien bestimmt. Hierbei konnten die höchsten Ausbeuten intakter Mitochondrien für einen Zellaufschluss mittels Ultraschall und anschließender differentieller Zentrifugation erzielt werden. Parallel zu den makroskopischen Methoden wurde als Kooperationsprojekt ein Mikrochipsystem entwickelt, das innerhalb weniger Sekunden eine vollständige Probenverarbeitung (einschließlich Zellaufschluss) für metabolische Analysen ermöglichen sollte. Der Prototyp des LoaC wurde innerhalb dieser Arbeit erfolgreich in einen Bioreaktor integriert und für eine Gesamtdauer von 18 h betrieben. Eine kontinuierliche Probenahme konnte hierbei mittels Überdruck realisiert werden. Neben dynamischen Pulsexperimenten wurde durch dieses Mikrochip-Bioreaktor-System eine effiziente Separation der Zellen vom extrazellulären Medium erzielt. Durch eine vorangestellte Zelllyse mittels Digitonin und die anschließende Abtrennung des freigesetzten Zytosols von den verbleibenden zellulären und mitochondrialen Kompartimenten, erwies das integrierte Mikrochipsystem großes Potential, um die Limitierungen zur Analyse des kompartimentierten Metabolismus zu überwinden. Im letzten Teil der Arbeit wurden die CHO-K1 Zellen für systembiologische Untersuchungen in einem geregelten Kleinmaßstab-bioreaktor kultiviert. Hierfür wurden neben Batch-Verfahren ebenfalls kontinuierliche Kultivierungen von Mischpopulationen durchgeführt und detaillierte extrazelluläre Metabolitanalysen mittels LC-MS vorgenommen. Darüber hinaus wurden durch konfokale und hochauflösende STED-Mikroskopie die Mitochondrienstrukturen und die Verteilung mitochondrialer Proteine in Abhängigkeit von den Kultivierungsbedingungen analysiert. Zudem konnte der relative Gehalt stoffwechselrelavanter Enzyme intrazellulär durch Einsatz der ELISA-Technologie bestimmt werden. Zur Untersuchung der Abhängigkeit meta-bolischer Reaktionen vom Zellzyklus wurden Kultivierungen synchronisierter Zellen erfolgreich durchgeführt. Hierzu wurden die Zellen im Vorfeld aus heterogenen Populationen mittels Elutriationszentrifugation in der G1- bzw. der S-Phase angereichert. Die Synchronität über den Zeitraum einer Batch-Kultivierung konnte mittels durchflusszytometrischer Analysen zur Bestimmung des DNA-Gehalts und des Zelldurchmessers sowie durch die erfolgreiche Applikation eines at-line Mikroskops zur kontinuierlichen Bestimmung der Zellzahl verfolgt werden. Metabolische und mikroskopische Untersuchungen synchronisierten Zellen wurden parallel dazu durchgeführt und mit den Daten der Kultivierungen von Mischpopulation verglichen. Unter anderem durch deutlich erhöhte Verbrauchsraten der Hauptsubstrate Glukose und L-Glutamin in der S-Phase und eine versetzt eintretende erhöhte Bildung von Laktat während der G1-Phase, konnte eine Zellzyklusabhängigkeit des Metabolismus gezeigt werden.']] | ['2014'] | ['http://id.loc.gov/vocabulary/iso639-1/de'] | ['open access'] | [[{'@id': 'gnd:4272013-8'}, {'@id': 'gnd:4803438-1'}, {'@id': 'gnd:4057699-1'}, {'@id': 'gnd:4124939-2'}, {'@id': 'gnd:4511937-5'}, {'@id': 'gnd:4464875-3'}]] | ['gnd:10193909-7', 'gnd:1138128279', 'gnd:12031830X', 'gnd:2067664-5', 'gnd:4057699-1', 'gnd:4124939-2', 'gnd:4272013-8', 'gnd:4464875-3', 'gnd:4511937-5', 'gnd:4803438-1', 'https://www.tib.eu/de/suchen/id/TIBKAT%3A782853390'] | ['Technische Universität Hamburg-Harburg, Institut für Bioprozess- und Biosystemtechnik', 'Zeng, An-Ping', 'Liese, Andreas', 'Technische Universität Hamburg-Harburg', 'Stoffwechsel', 'Mitochondrium', 'Zellaufschluss', 'Systemintegration', 'Online-Ressource', 'Mikrofluidik'] | 4 |
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